LOCUS       pEK8                    5218 bp    DNA     circular UNA 11-JAN-2021
DEFINITION  A new nucleotide sequence entered manually.
ACCESSION   urn.local...d-cvr4q51
VERSION     urn.local...d-cvr4q51
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        complement(469..497)
                     /note="Geneious type: Promoter"
     misc_feature    complement(690..996)
                     /note="Geneious type: Origin of Replication"
                     /standard_name="f1 origin"
     misc_feature    complement(1014..1174)
                     /note="Geneious type: Reporter"
                     /standard_name="lacZ_a reporter"
     promoter        1181..1199
                     /note="Geneious type: Promoter"
     gene            1300..3165
                     /standard_name="AAV-2_rep"
     promoter        3370..3466
     promoter        complement(3584..3603)
                     /note="Geneious type: Promoter"
     promoter        complement(3673..3702)
                     /note="Geneious type: Promoter"
     misc_feature    complement(4011..4630)
                     /note="Geneious type: Origin of Replication"
                     /standard_name="pBR322 origin"
     misc_feature    complement(4785..427)
                     /note="Geneious type: Marker"
                     /standard_name="amp marker"
ORIGIN      
        1 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
       61 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
      121 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
      181 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
      241 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
      301 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
      361 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
      421 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
      481 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
      541 cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
      601 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
      661 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
      721 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
      781 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
      841 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
      901 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
      961 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc
     1021 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     1081 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     1141 ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact cactataggg
     1201 cgaattgggt accgggcccc ccctcgatcg aggtcgacgg tatcggggga gctcgcaggg
     1261 tctccatttt gaagcgggag gtttgaacgc gcagccgcca tgccggggtt ttacgagatt
     1321 gtgattaagg tccccagcga ccttgacgag catctgcccg gcatttctga cagctttgtg
     1381 aactgggtgg ccgagaagga atgggagttg ccgccagatt ctgacatgga tctgaatctg
     1441 attgagcagg cacccctgac cgtggccgag aagctgcagc gcgactttct gacggaatgg
     1501 cgccgtgtga gtaaggcccc ggaggctctt ttctttgtgc aatttgagaa gggagagagc
     1561 tacttccaca tgcacgtgct cgtggaaacc accggggtga aatccatggt tttgggacgt
     1621 ttcctgagtc agattcgcga aaaactgatt cagagaattt accgcgggat cgagccgact
     1681 ttgccaaact ggttcgcggt cacaaagacc agaaatggcg ccggaggcgg gaacaaggtg
     1741 gtggatgagt gctacatccc caattacttg ctccccaaaa cccagcctga gctccagtgg
     1801 gcgtggacta atatggaaca gtatttaagc gcctgtttga atctcacgga gcgtaaacgg
     1861 ttggtggcgc agcatctgac gcacgtgtcg cagacgcagg agcagaacaa agagaatcag
     1921 aatcccaatt ctgatgcgcc ggtgatcaga tcaaaaactt cagccaggta catggagctg
     1981 gtcgggtggc tcgtggacaa ggggattacc tcggagaagc agtggatcca ggaggaccag
     2041 gcctcataca tctccttcaa tgcggcctcc aactcgcggt cccaaatcaa ggctgccttg
     2101 gacaatgcgg gaaagattat gagcctgact aaaaccgccc ccgactacct ggtgggccag
     2161 cagcccgtgg aggacatttc cagcaatcgg atttataaaa ttttggaact aaacgggtac
     2221 gatccccaat atgcggcttc cgtctttctg ggatgggcca cgaaaaagtt cggcaagagg
     2281 aacaccatct ggctgtttgg gcctgcaact accgggaaga ccaacatcgc ggaggccata
     2341 gcccacactg tgcccttcta cgggtgcgta aactggacca atgagaactt tcccttcaac
     2401 gactgtgtcg acaagatggt gatctggtgg gaggagggga agatgaccgc caaggtcgtg
     2461 gagtcggcca aagccattct cggaggaagc aaggtgcgcg tggaccagaa atgcaagtcc
     2521 tcggcccaga tagacccgac tcccgtgatc gtcacctcca acaccaacat gtgcgccgtg
     2581 attgacggga actcaacgac cttcgaacac cagcagccgt tgcaagaccg gatgttcaaa
     2641 tttgaactca cccgccgtct ggatcatgac tttgggaagg tcaccaagca ggaagtcaaa
     2701 gactttttcc ggtgggcaaa ggatcacgtg gttgaggtgg agcatgaatt ctacgtcaaa
     2761 aagggtggag ccaagaaaag acccgccccc agtgacgcag atataagtga gcccaaacgg
     2821 gtgcgcgagt cagttgcgca gccatcgacg tcagacgcgg aagcttcgat caactacgca
     2881 gacaggtacc aaaacaaatg ttctcgtcac gtgggcatga atctgatgct gtttccctgc
     2941 agacaatgcg agagaatgaa tcagaattca aatatctgct tcactcacgg acagaaagac
     3001 tgtttagagt gctttcccgt gtcagaatct caacccgttt ctgtcgtcaa aaaggcgtat
     3061 cagaaactgt gctacattca tcatatcatg ggaaaggtgc cagacgcttg cactgcctgc
     3121 gatctggtca atgtggattt ggatgactgc atctttgaac aataaatgat ttaaatcagg
     3181 tatggctgcc gatggttatc ttccagattg gctcgaggac actctctctg aaggaataag
     3241 acagtggtgg aagctcaaac ctggcccacc atgataataa ttgcctgtta atcaataaac
     3301 cggttgattc gtttcagttg aactttggtc tctgcgaagg gcgaattcgt ttaaacctgc
     3361 aggactagag gtcctgtatt agaggtcacg tgagtgtttt gcgacatttt gcgacaccat
     3421 gtggtcacgc tgggtattta agcccgagtg agcacgcagg gtctccattt tgaagcggga
     3481 ggtttgaacg cgcagccgcc aagccgaatt ctgcagatat ccatcacact ggcggccgct
     3541 cgactagagc ggccgccacc gcggtggagc tccagctttt gttcccttta gtgagggtta
     3601 attgcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     3661 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     3721 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     3781 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     3841 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     3901 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     3961 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     4021 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     4081 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     4141 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     4201 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     4261 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     4321 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     4381 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     4441 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     4501 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     4561 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     4621 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     4681 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     4741 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     4801 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     4861 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     4921 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     4981 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     5041 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     5101 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     5161 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcg
//
