LOCUS       Exported                4394 bp ds-DNA     circular UNK 22-JUN-2022
DEFINITION  natural circular DNA
ACCESSION   <unknown
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Mar 13, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     ncRNA           complement(967..1070)
                     /label=RNAI
                     /note="pLannotate"
                     /note="/database=infernal"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=102.94117647058823"
                     /note="/other=ncRNA"
     rep_origin      1166..1540
                     /label=R6K gamma ori
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=99.734"
                     /note="/match_length=96.90721649484536"
                     /note="/other=rep_origin"
     oriT            complement(1573..1680)
                     /direction=LEFT
                     /label=oriT
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=oriT"
     promoter        1941..1974
                     /label=J23119 promoter
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=97.143"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     primer_bind     1996..2013
                     /label=Primer 1
     CDS             2073..2774
                     /label=superfolder GFP
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=98.459"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     primer_bind     complement(2779..2797)
                     /label=Primer 2
     CDS             2864..3558
                     /label=mScarlet
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     terminator      3563..3633
                     /label=rrnB T1 terminator
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
     terminator      complement(3650..3676)
                     /label=T7Te terminator
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
     CDS             3694..3854
                     /label=lacZ
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=5.2682926829268295"
                     /note="/other=CDS"
     misc_feature    complement(3855..3953)
                     /label=bom
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=71.94244604316546"
                     /note="/other=misc feature"
     CDS             complement(4056..4090)
                     /label=rop
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=19.047619047619047"
                     /note="/other=CDS"
     promoter        4186..4289
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             join(4291..4394,1..756)
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
ORIGIN
        1 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
       61 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
      121 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
      181 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
      241 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
      301 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
      361 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
      421 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
      481 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
      541 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
      601 cggtgagcgt ggctctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
      661 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
      721 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
      781 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
      841 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
      901 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
      961 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     1021 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     1081 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     1141 tctgctaatc ctgttaccag ccggttgtca gccgttaagt gttcctgtgt cactcaaaat
     1201 tgctttgaga ggctctaagg gcttctcagt gcgttacatc cctggcttgt tgtccacaac
     1261 cgttaaacct taaaagcttt aaaagcctta tatattcttt tttttcttat aaaacttaaa
     1321 accttagagg ctatttaagt tgctgattta tattaatttt attgttcaaa catgagagct
     1381 tagtacgtga aacatgagag cttagtacgt tagccatgag agcttagtac gttagccatg
     1441 agggtttagt tcgttaaaca tgagagctta gtacgttaaa cttgagagct tagtacgtga
     1501 aacatgagag cttagtacgt actatcaaca ggttgaactg cccatgttct ttcctgcgtt
     1561 atcagagctt atcggccagc ctcgcagagc aggattcccg ttgagcaccg ccaggtgcga
     1621 ataagggaca gtgaagaagg aacacccgct cgcgggtggg cctacttcac ctatcctgcc
     1681 cggctgacgc cgttggatac accaaggaaa gtctacacga accctttggc aaaatcctgt
     1741 atatcgtgcg aaaaaggatg gatataccga aaaaatcgct ataatgaccc cgaagcaggg
     1801 ttatgcagcg gaaagtatac cttaaatgtt ctttcctgcg ttatcccctg attctgtgga
     1861 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     1921 cagcgagtca gtgagcgagg ttgacagcta gctcagtcct aggtataata ctagcgtcga
     1981 cgtggagata taccaaacga gagacccatc gcggcaactc aagaaacggc aggttcggat
     2041 cttagctact agagaaagag gagaaatact agatgcgtaa aggcgaagag ctgttcactg
     2101 gtgtcgtccc tattctggtg gaactggatg gtgatgtcaa cggtcataag ttttccgtgc
     2161 gtggcgaggg tgaaggtgac gcaactaatg gtaaactgac gctgaagttc atctgtacta
     2221 ctggtaaact gccggttcct tggccgactc tggtaacgac gctgacttat ggtgttcagt
     2281 gctttgctcg ttatccggac catatgaagc agcatgactt cttcaagtcc gccatgccgg
     2341 aaggctatgt gcaggaacgc acgatttgac ggcacgtaca aaacgcgtgc ggaagtgaaa
     2401 tttgaaggcg ataccctggt aaaccgcatt gagctgaaag gcattgactt taaagaggac
     2461 ggcaatatcc tgggccataa gctggaatac aattttaaca gccacaatgt ttacatcacc
     2521 gccgataaac aaaaaaatgg cattaaagcg aattttaaaa ttcgccacaa cgtggaggat
     2581 ggcagcgtgc agctggctga tcactaccag caaaacactc caatcggtga tggtcctgtt
     2641 ctgctgccag acaatcacta tctgagcacg caaagcgttc tgtctaaaga tccgaacgag
     2701 aaacgcgatc atatggttct gctggagttc gtaaccgcag cgggcatcac gcatggtatg
     2761 gatgaactgt acaaatgacc aggcatggtc tcctatggat ccgaattcga gcgaaggaga
     2821 tatacatccc tttaagcagg tgtttcaatt aaggaggttt tttatggtga gcaagggcga
     2881 ggcagtgatc aaggagttca tgcggttcaa ggtgcacatg gagggctcca tgaacggcca
     2941 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
     3001 gctgaaggtg accaagggtg gccccctgcc cttctcctgg gacatcctgt cccctcagtt
     3061 catgtacggc tccagggcct tcaccaagca ccccgccgac atccccgact actataagca
     3121 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgccgt
     3181 gaccgtgacc caggacacct ccctggagga cggcaccctg atctacaagg tgaagctccg
     3241 cggcaccaac ttccctcctg acggccccgt aatgcagaag aagacaatgg gctgggaagc
     3301 gtccaccgag cggttgtacc ccgaggacgg cgtgctgaag ggcgacatta agatggccct
     3361 gcgcctgaag gacggcggcc gctacctggc ggacttcaag accacctaca aggccaagaa
     3421 gcccgtgcag atgcccggcg cctacaacgt cgaccgcaag ttggacatca cctcccacaa
     3481 cgaggactac accgtggtgg aacagtacga acgctccgag ggccgccact ccaccggcgg
     3541 catggacgag ctgtacaagt gacaaataaa acgaaaggct cagtcgaaag actgggcctt
     3601 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc
     3661 gggtgggcct ttctgcgcac ctgcatgcta tgatcactgg ccgtcgtttt acaacgtcgt
     3721 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     3781 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     3841 aatggcgaat ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     3901 cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     3961 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     4021 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     4081 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     4141 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     4201 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     4261 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     4321 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     4381 gtaaaagatg ctga
//
