LOCUS       AAV_pSyn_mito_LO        5189 bp    DNA        linear       09-MAR-2023
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    730..2091
                     /locus_tag="LOV-Turbo (codon not optimized)"
                     /label="LOV-Turbo (codon not optimized)"
                     /ApEinfo_label="LOV-Turbo (codon not optimized)"
                     /ApEinfo_fwdcolor="#fefb00"
                     /ApEinfo_revcolor="#ffd478"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1..130
                     /locus_tag="ITR"
                     /label="ITR"
                     /ApEinfo_label="ITR"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    151..628
                     /locus_tag="synapsin promoter"
                     /label="synapsin promoter"
                     /ApEinfo_label="synapsin promoter"
                     /ApEinfo_fwdcolor="#4eff51"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2453..2593
                     /locus_tag="ITR(1)"
                     /label="ITR(1)"
                     /ApEinfo_label="ITR"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2193..2424
                     /locus_tag="PolyA"
                     /label="PolyA"
                     /ApEinfo_label="PolyA"
                     /ApEinfo_fwdcolor="#ff2549"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    649..651
                     /locus_tag="Ig k-chain signal sequence"
                     /label="Ig k-chain signal sequence"
                     /ApEinfo_label="Ig k-chain signal sequence"
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(2140..2142)
                     /locus_tag="double stop"
                     /label="double stop"
                     /ApEinfo_label="double stop"
                     /ApEinfo_fwdcolor="#800080"
                     /ApEinfo_revcolor="#800080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2092..2097
                     /locus_tag="NheI"
                     /label="NheI"
                     /ApEinfo_label="NheI"
                     /ApEinfo_fwdcolor="#ff8040"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    637..642
                     /locus_tag="HindIII"
                     /label="HindIII"
                     /ApEinfo_label="HindIII"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    652..720
                     /locus_tag="Mito2"
                     /label="Mito2"
                     /ApEinfo_label="Mito2"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    723..728
                     /locus_tag="BamHI"
                     /label="BamHI"
                     /ApEinfo_label="BamHI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2098..2139
                     /locus_tag="V5 tag"
                     /label="V5 tag"
                     /ApEinfo_label="V5 tag"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             955..1371
                     /locus_tag="hLOV1"
                     /label="hLOV1"
                     /ApEinfo_label="hLOV1"
                     /ApEinfo_fwdcolor="#83c5fa"
                     /ApEinfo_revcolor="#319df7"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    880..882
                     /locus_tag="T52S"
                     /label="T52S"
                     /ApEinfo_label="T52S"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    943..945
                     /locus_tag="L73Q"
                     /label="L73Q"
                     /ApEinfo_label="L73Q"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1567..1569
                     /locus_tag="I147T"
                     /label="I147T"
                     /ApEinfo_label="I147T"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1624..1626
                     /locus_tag="A166V"
                     /label="A166V"
                     /ApEinfo_label="A166V"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1819..1821
                     /locus_tag="I231V"
                     /label="I231V"
                     /ApEinfo_label="I231V"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1984..1986
                     /locus_tag="F286L"
                     /label="F286L"
                     /ApEinfo_label="F286L"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtTGTACA gtgtctagac tgcagagggc cctgcgtatg
      181 agtgcaagtg ggttttagga ccaggatgag gcggggtggg ggtgcctacc tgacgaccga
      241 ccccgaccca ctggacaagc acccaacccc cattccccaa attgcgcatc ccctatcaga
      301 gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt cagcaccgcg
      361 gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc
      421 gctgacgtca ctcgccggtc ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg
      481 cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg
      541 cgaccatctg cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg
      601 aggagtcgtg tcgtgcctga gagcgcagtc gagaagaagc ttGCCACCat gctggccacc
      661 cgcgtgttca gcctggtggg caagcgcgcc atcagcacca gcgtgtgcgt gcgcgcccac
      721 aaggatccaa aagacaatac tgtgcctctg aagctgatcg ctctcctggc taatggcgag
      781 ttccatagtg gcgaacagct gggagaaacc ctgggcatgt ccagggccgc tatcaacaag
      841 cacattcaga ctctgcgcga ctggggcgtg gacgtgttcT ccgtgcccgg aaagggctac
      901 tctctgcccg agcctatccc gctgctgaac gctaaacaga ttcAgggaCA GCTGGAGTTT
      961 cggGCAACCA CACTGGAACG GATCGAGAAA agcTTCGTGA TTACTGATCC GAGACTGCCT
     1021 GACAACCCAA TCATTTTTgt gAGCGATTCC TTCCTGCAGC TGACAGAATA TTCTCGGGAA
     1081 GAGATCCTGG GGCGCAATTG CCGTTTTCTG CAGGGACCCG AGACAGACCG TGCCACTGTT
     1141 CGGAAAATCA GAGATGCTAT TGACAACCAG ACTGAAGTGA CCGTTCAGCT GATCAATTAT
     1201 ACCAAGAGCG GCAAGAAGTT CTGGAACGTG TTCCACCTGC AGCCGATGCG CGATTATAAG
     1261 GGCGACGTCC AGTACTTCAT TGGCGTGCAG CTGGATGGCA CCGAACGTCT TCATGGCGCC
     1321 GCTGAGCGTG AGGCGGTCTG CCTGgtcAAA AAGACAGCCT TTCAGATTGC Tgtggcagtc
     1381 ctgcctgtgg tcgactccac caatcagtac ctgctggatc gaatcggcga gctgaagagt
     1441 ggggatgctt gcattgcaga atatcagcag gcagggagag gaagcagagg gaggaaatgg
     1501 ttctctcctt ttggagctaa cctgtacctg agtatgtttt ggcgcctgaa gcggggacca
     1561 gcagcaaCcg gcctgggccc ggtcatcgga attgtcatgg cagaagcgct gcgaaagctg
     1621 ggagTagaca aggtgcgagt caaatggccc aatgacctgt atctgcagga tagaaagctg
     1681 gcaggcatcc tggtggagct ggccggaata acaggcgatg ctgcacagat cgtcattggc
     1741 gccgggatta acgtggctat gaggcgcgtg gaggaaagcg tggtcaatca gggctggatc
     1801 acactgcagg aagcagggGt taacctggac aggaatactc tggccgctac gctgatccga
     1861 gagctgcggg cagccctgga actgttcgag caggaaggcc tggctccata tctgccacgg
     1921 tgggagaagc tggataactt catcaataga cccgtgaagc tgatcattgg ggacaaagag
     1981 attCtcggga ttagccgggg gattgaCaaa cagggagccc tgctgctgga acaggacgga
     2041 gttatcaaac cctggatggg cggagaaatc agtctgcggt ctgccgaaaa ggctagcggc
     2101 aagcccatcc ccaaccccct gctgggcctg gacagcaccT AATGACTCGA GCATGCATCT
     2161 AGAGGGCCCT ATTCTATAGT GTCACCTAAA TGCTAGAGCT CGCTGATCAG CCTCGACTGT
     2221 GCCTTCTAGT TGCCAGCCAT CTGTTGTTTG CCCCTCCCCC GTGCCTTCCT TGACCCTGGA
     2281 AGGTGCCACT CCCACTGTCC TTTCCTAATA AAATGAGGAA ATTGCATCGC ATTGTCTGAG
     2341 TAGGTGTCAT TCTATTCTGG GGGGTGGGGT GGGGCAGGAC AGCAAGGGGG AGGATTGGGA
     2401 AGACAATAGC AGGCATGCTG GGGAggtaac cacgtgcgga ccgagcggcc gcaggaaccc
     2461 ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga
     2521 ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc
     2581 agctgcctgc aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtattcac
     2641 accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg
     2701 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     2761 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     2821 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     2881 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     2941 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     3001 tatctcgggc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     3061 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat
     3121 tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     3181 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     3241 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     3301 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     3361 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     3421 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     3481 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     3541 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     3601 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     3661 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     3721 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     3781 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     3841 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     3901 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     3961 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     4021 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     4081 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     4141 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     4201 taaatctgga gccggtgagc gtggaagccg cggtatcatt gcagcactgg ggccagatgg
     4261 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     4321 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     4381 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     4441 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     4501 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     4561 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     4621 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     4681 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     4741 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     4801 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     4861 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     4921 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     4981 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     5041 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     5101 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     5161 ggccttttgc tggccttttg ctcacatgt   
//
