LOCUS       pcDNA3_myc_arres        8057 bp    DNA        circular     09-MAR-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8057)
  AUTHORS   Heegwang Roh
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jul 18, 2022 from SnapGene Viewer 5.1.3.1
            https://www.snapgene.com
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     source          join(1..2228,3591..8057)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /locus_tag="source:synthetic DNA construct"
                     /label="source:synthetic DNA construct"
                     /ApEinfo_label="source:synthetic DNA construct"
                     /ApEinfo_fwdcolor="#00DF87"
                     /ApEinfo_revcolor="#00DF87"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /ApEinfo_hidden
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /locus_tag="CMV enhancer"
                     /label="CMV enhancer"
                     /ApEinfo_label="CMV enhancer"
                     /ApEinfo_fwdcolor="#5ac3fa"
                     /ApEinfo_revcolor="#5ac3fa"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /locus_tag="CMV promoter"
                     /label="CMV promoter"
                     /ApEinfo_label="CMV promoter"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     promoter        863..881
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /locus_tag="T7 promoter"
                     /label="T7 promoter"
                     /ApEinfo_label="T7 promoter"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             906..935
                     /codon_start="1"
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /translation="EQKLISEEDL"
                     /locus_tag="Myc"
                     /label="Myc"
                     /ApEinfo_label="Myc"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    972..2198
                     /locus_tag="Arrestin"
                     /label="Arrestin"
                     /ApEinfo_label="Arrestin"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2199..2228
                     /locus_tag="10aa linker"
                     /label="10aa linker"
                     /ApEinfo_label="10aa linker"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     promoter        complement(3609..3627)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /locus_tag="SP6 promoter"
                     /label="SP6 promoter"
                     /ApEinfo_label="SP6 promoter"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     polyA_signal    3653..3877
                     /note="bovine growth hormone polyadenylation signal"
                     /locus_tag="bGH poly(A) signal"
                     /label="bGH poly(A) signal"
                     /ApEinfo_label="bGH poly(A) signal"
                     /ApEinfo_fwdcolor="#ff3eee"
                     /ApEinfo_revcolor="#ff3eee"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     rep_origin      3923..4351
                     /direction="RIGHT"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /locus_tag="f1 ori"
                     /label="f1 ori"
                     /ApEinfo_label="f1 ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     promoter        4365..4695
                     /note="SV40 enhancer and early promoter"
                     /locus_tag="SV40 promoter"
                     /label="SV40 promoter"
                     /ApEinfo_label="SV40 promoter"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     rep_origin      4546..4681
                     /note="SV40 origin of replication"
                     /locus_tag="SV40 ori"
                     /label="SV40 ori"
                     /ApEinfo_label="SV40 ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             4762..5556
                     /codon_start="1"
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
                     /locus_tag="NeoR/KanR"
                     /label="NeoR/KanR"
                     /ApEinfo_label="NeoR/KanR"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     polyA_signal    5730..5851
                     /note="SV40 polyadenylation signal"
                     /locus_tag="SV40 poly(A) signal"
                     /label="SV40 poly(A) signal"
                     /ApEinfo_label="SV40 poly(A) signal"
                     /ApEinfo_fwdcolor="#ff3eee"
                     /ApEinfo_revcolor="#ff3eee"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(5900..5916)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /locus_tag="M13 rev"
                     /label="M13 rev"
                     /ApEinfo_label="M13 rev"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     protein_bind    5924..5940
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /locus_tag="lac operator"
                     /label="lac operator"
                     /ApEinfo_label="lac operator"
                     /ApEinfo_fwdcolor="#00D95A"
                     /ApEinfo_revcolor="#00D95A"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     promoter        complement(5948..5978)
                     /note="promoter for the E. coli lac operon"
                     /locus_tag="lac promoter"
                     /label="lac promoter"
                     /ApEinfo_label="lac promoter"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     protein_bind    5993..6014
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /locus_tag="CAP binding site"
                     /label="CAP binding site"
                     /ApEinfo_label="CAP binding site"
                     /ApEinfo_fwdcolor="#008065"
                     /ApEinfo_revcolor="#008065"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     rep_origin      complement(6302..6890)
                     /direction="LEFT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /locus_tag="ori"
                     /label="ori"
                     /ApEinfo_label="ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             complement(7061..7921)
                     /codon_start="1"
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     promoter        complement(7922..8026)
                     /gene="bla"
                     /locus_tag="AmpR promoter"
                     /label="AmpR promoter"
                     /ApEinfo_label="AmpR promoter"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2229..3590
                     /locus_tag="LOV-Turbo, human codon optimized"
                     /label="LOV-Turbo, human codon optimized"
                     /ApEinfo_label="LOV-Turbo, human codon optimized"
                     /ApEinfo_fwdcolor="#fefb00"
                     /ApEinfo_revcolor="#ffd478"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    3318..3320
                     /locus_tag="I231V"
                     /label="I231V"
                     /ApEinfo_label="I231V"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2379..2381
                     /locus_tag="T52S"
                     /label="T52S"
                     /ApEinfo_label="T52S"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2442..2444
                     /locus_tag="L73Q"
                     /label="L73Q"
                     /ApEinfo_label="L73Q"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2454..2870
                     /locus_tag="LOV"
                     /label="LOV"
                     /ApEinfo_label="LOV"
                     /ApEinfo_fwdcolor="#0096ff"
                     /ApEinfo_revcolor="#0432ff"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    3066..3068
                     /locus_tag="I147T"
                     /label="I147T"
                     /ApEinfo_label="I147T"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3123..3125
                     /locus_tag="A166V"
                     /label="A166V"
                     /ApEinfo_label="A166V"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3483..3485
                     /locus_tag="F286L"
                     /label="F286L"
                     /ApEinfo_label="F286L"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttgcggcc
      901 gcatggaaca aaagcttatt tctgaagagg acttgggctc gggcgggagt accggtggta
      961 gtgggagcgg tggtgaaaaa cccgggacca gggtcttcaa gaagtcgagc cctaactgca
     1021 agctcaccgt gtacttgggc aagcgtgact ttgtggatca cttggacaaa gtggatcctg
     1081 tcgatggtgt ggtgcttgtg gatcctgact acttgaagga ccggaaagtg tttgtgaccc
     1141 tcacctgtgc cttccgctat ggccgagaag acctggatgt actgggcctg tctttccgca
     1201 aagatctgtt catcgccacc taccaggcct tcccccccat gcccaaccca cctcggcccc
     1261 ccacccgcct acaggaccga ctgctgaaga agttgggcca gcacgcccac cccttttttt
     1321 tcacaatacc ccagaatttg ccttgctccg tcacactgca gccaggaccg gaggacacag
     1381 ggaaggcctg tggagtagac tttgagattc gagccttctg tgccaaatct atagaagaaa
     1441 aaagccacaa aaggaactcc gtgcggctta tcatcagaaa ggtacagttt gctcctgaga
     1501 cacccggccc ccagccatca gctgaaacca cacgccactt cctcatgtct gaccggaggt
     1561 ccctgcacct agaggcttcc ctggacaaag agctgtacta ccatggggaa cccctcaatg
     1621 tcaacgtcca cgtcaccaac aattctgcca aaaccgtcaa gaagatcaga gtgtctgtga
     1681 gacagtatgc cgacatttgc ctcttcagca ccgcgcagta caagtgtcct gtggctcagc
     1741 ttgaacaaga tgaccaggtg tctcccagtt ccacattctg caaggtgtac accataaccc
     1801 cgctgctcag tgacaaccga gagaagcgtg gccttgccct tgatgggcaa ctcaagcacg
     1861 aagacaccaa cctggcttcc agcaccattg tgaaggaggg agccaacaag gaggtgctgg
     1921 gaatcctagt atcctacagg gtcaaggtga agctggtggt gtctcgaggc ggggatgtct
     1981 ccgtggagct acctttcgtc ctaatgcacc ccaagcccca cgaccacatc acccttcccc
     2041 gaccccagtc agccccccgg gaaatagaca tccctgtgga taccaacctc attgaattcg
     2101 ataccaacta tgccacagac gacgacatcg tgtttgagga ctttgcgagg cttcggctga
     2161 aggggatgaa ggatgacgac tgtgatgacc agttctgcgg tggaagtgga tcaggcagcg
     2221 gtggatctAA AGACAACACA GTGCCTCTGA AGCTGATCGC CCTCCTGGCC AATGGAGAGT
     2281 TCCACAGCGG CGAGCAGCTG GGCGAAACCC TGGGCATGAG CAGAGCCGCT ATCAACAAGC
     2341 ACATCCAGAC CCTTCGCGAC TGGGGCGTCG ACGTGTTCAG CGTGCCCGGC AAGGGCTACA
     2401 GCCTGCCTGA GCCTATCCCC CTGCTGAACG CCAAGCAGAT TCAGGGCCAG CTGGAATTTA
     2461 GAGCCACCAC CCTGGAAAGA ATCGAGAAGT CTTTTGTGAT CACCGACCCC AGACTGCCTG
     2521 ATAATCCTAT CATCTTCGTG TCTGATAGCT TCCTGCAGCT CACCGAGTAT TCTCGGGAAG
     2581 AGATCCTCGG AAGAAATTGC AGATTCCTGC AGGGCCCAGA GACAGATAGA GCCACAGTGC
     2641 GGAAGATCAG AGATGCCATC GACAACCAGA CAGAGGTGAC CGTGCAGCTG ATCAACTACA
     2701 CCAAGTCCGG CAAAAAGTTC TGGAACGTTT TCCATCTCCA ACCTATGAGA GACTACAAGG
     2761 GCGACGTGCA GTACTTCATC GGAGTTCAGC TGGACGGCAC CGAGAGACTG CACGGCGCCG
     2821 CAGAGCGGGA AGCTGTGTGC CTGGTCAAGA AGACCGCCTT CCAGATCGCC GTGGCTGTGC
     2881 TGCCCGTGGT GGACAGCACA AACCAGTACC TGCTGGACAG AATCGGCGAG CTGAAGTCCG
     2941 GCGACGCTTG TATCGCCGAA TACCAGCAGG CCGGCAGAGG CAGCAGAGGC CGGAAATGGT
     3001 TCAGCCCCTT CGGCGCCAAC CTGTACCTGA GCATGTTTTG GCGGCTGAAA AGAGGCCCTG
     3061 CCGCCACAGG CCTGGGCCCC GTGATAGGAA TCGTGATGGC CGAGGCTCTG AGGAAGCTGG
     3121 GTGTGGACAA AGTGCGGGTG AAGTGGCCTA ACGACCTGTA CCTGCAGGAC AGAAAGCTGG
     3181 CCGGCATCCT GGTGGAACTG GCTGGCATCA CCGGCGACGC CGCTCAGATT GTGATCGGCG
     3241 CTGGCATCAA TGTGGCCATG AGACGCGTGG AAGAGAGCGT GGTCAACCAG GGCTGGATCA
     3301 CACTGCAAGA GGCCGGTGTG AACCTGGATA GGAACACCCT GGCCGCCACC CTGATCAGAG
     3361 AGCTGAGAGC CGCTCTGGAA TTGTTTGAGC AGGAGGGCCT GGCCCCTTAC CTGCCACGGT
     3421 GGGAGAAACT GGATAACTTC ATCAACAGGC CTGTGAAGCT GATCATCGGC GATAAGGAAA
     3481 TCCTGGGAAT CAGCCGGGGC ATCGACAAGC AGGGAGCCCT GCTGCTGGAA CAAGATGGAG
     3541 TGATTAAGCC TTGGATGGGC GGAGAAATCA GCCTGCGGTC CGCCGAGAAG taatctagag
     3601 ggccctattc tatagtgtca cctaaatgct agagctcgct gatcagcctc gactgtgcct
     3661 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     3721 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     3781 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     3841 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     3901 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     3961 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     4021 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggc
     4081 atccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     4141 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     4201 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     4261 tcggtctatt cttttgattt ataagggatt ttggggattt cggcctattg gttaaaaaat
     4321 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt
     4381 gtggaaagtc cccaggctcc ccaggcaggc agaagtatgc aaagcatgca tctcaattag
     4441 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     4501 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     4561 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     4621 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     4681 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag
     4741 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
     4801 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     4861 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     4921 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg
     4981 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     5041 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     5101 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     5161 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     5221 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     5281 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     5341 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     5401 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     5461 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     5521 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga
     5581 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg
     5641 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     5701 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca
     5761 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     5821 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
     5881 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     5941 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     6001 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     6061 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     6121 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     6181 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     6241 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     6301 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     6361 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     6421 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     6481 gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     6541 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     6601 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     6661 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     6721 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     6781 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     6841 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     6901 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     6961 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     7021 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     7081 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     7141 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     7201 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     7261 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     7321 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     7381 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     7441 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     7501 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     7561 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     7621 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     7681 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     7741 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     7801 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     7861 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     7921 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     7981 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     8041 aagtgccacc tgacgtc    
//
