LOCUS       Exported                5513 bp ds-DNA     circular SYN 14-NOV-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5513)
  AUTHORS   Heegwang Roh
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jul 18, 2022 from SnapGene Viewer 5.1.3.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5513
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        181..484
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        485..688
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        733..751
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    771..1892
                     /label=CCR6
     CDS             1947..2456
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="VFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRI
                     VLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVT
                     PNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWR
                     LCERILA"
     CDS             2457..2498
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     polyA_signal    2541..2748
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2777..2917
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      2991..3446
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3728..3832
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3833..4693
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4864..5452
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
      181 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      241 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      301 atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      361 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      421 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      481 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      541 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      601 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      661 acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
      721 gcttatcgaa attaatacga ctcactatag ggagacccaa gcttgccacc atgagcggag
      781 agagcatgaa cttcagtgat gtgttcgatt ccagtgagga ctacttcgtg tccgtcaata
      841 catcctacta cagcgtagat tctgagatgc tgctttgctc cctgcaggag gtgcgccagt
      901 tctcccgact cttcgttccc atcgcgtatt cactcatctg cgtctttggc ctcctgggga
      961 atatcttggt agtcatcaca tttgcttttt ataaaaaggc cagatccatg acagacgtct
     1021 acctgcttaa tatggccatt gcagacatcc tgttcgtcct gacgctccca ttctgggctg
     1081 tgagtcacgc taccggggcc tgggtgttca gtaatgcaac ctgcaagctg ttgaagggaa
     1141 tttatgccat caactttaat tgcggtatgc tgctgctgac ttgcataagt atggacagat
     1201 acatcgccat agtacaggca acaaaatcct tcagactgag gtctcgaacg ctccctaggt
     1261 ctaaaatcat ttgtcttgtt gtatggggct tgagcgtgat tatttccagc agtactttcg
     1321 tgtttaacca gaagtacaac acgcagggca gcgatgtgtg tgagcccaag taccagacgg
     1381 tatctgagcc tatcaggtgg aagctgttga tgctgggttt ggaattgctg ttcggctttt
     1441 ttatcccact catgtttatg attttttgtt ataccttcat cgtgaagacc ctggtccagg
     1501 cacaaaactc taagcgacac aaggccatcc gggtaatcat cgctgttgtg ctggtatttc
     1561 tggcttgcca gatccctcat aatatggtcc ttctggtaac ggcagccaac ctgggcaaaa
     1621 tgaatcgctc atgtcagtca gaaaagctta tcgggtacac caaaacggtt acggaggttc
     1681 tggcgttcct gcattgttgc cttaaccctg tgctgtacgc atttataggt cagaagtttc
     1741 gaaactactt cctgaagatt ctcaaagacc tctggtgtgt aagaagaaag tataaaagta
     1801 gtggattttc atgcgcagga cgctactccg aaaacatttc cagacagacc agtgagacgg
     1861 ccgataacga taacgcctcc tcctttacca tgactagtga gctggccgag aagctggccg
     1921 gcctggacat caacggtgga gctagcgtct tcacactcga agatttcgtt ggggactggc
     1981 gacagacagc cggctacaac ctggaccaag tccttgaaca gggaggtgtg tccagtttgt
     2041 ttcagaatct cggggtgtcc gtaactccga tccaaaggat tgtcctgagc ggtgaaaatg
     2101 ggctgaagat cgacatccat gtcatcatcc cgtatgaagg tctgagcggc gaccaaatgg
     2161 gccagatcga aaaaattttt aaggtggtgt accctgtgga tgatcatcac tttaaggtga
     2221 tcctgcacta tggcacactg gtaatcgacg gggttacgcc gaacatgatc gactatttcg
     2281 gacggccgta tgaaggcatc gccgtgttcg acggcaaaaa gatcactgta acagggaccc
     2341 tgtggaacgg caacaaaatt atcgacgagc gcctgatcaa ccccgacggc tccctgctgt
     2401 tccgagtaac catcaacgga gtgaccggct ggcggctgtg cgaacgcatt ctggcgggca
     2461 agccgatccc taaccctctg ctgggcctgg acagcactta gtaagaattc ctcgagctag
     2521 agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     2581 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     2641 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     2701 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggagg taaccacgtg
     2761 cggaccgagc ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct
     2821 cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg
     2881 gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc
     2941 cttacgcatc tgtgcggtat tcacaccgca tacgtcaaag caaccatagt acgcgccctg
     3001 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     3061 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     3121 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     3181 gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg
     3241 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     3301 ccaaactgga acaacactca accctatctc gggctattct tttgatttat aagggatttt
     3361 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     3421 taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc
     3481 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     3541 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     3601 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
     3661 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     3721 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     3781 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     3841 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     3901 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     3961 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     4021 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     4081 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     4141 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     4201 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     4261 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     4321 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     4381 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     4441 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     4501 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtggaa gccgcggtat
     4561 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     4621 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     4681 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     4741 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     4801 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     4861 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     4921 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     4981 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     5041 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     5101 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     5161 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     5221 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     5281 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     5341 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     5401 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     5461 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgt
//
