LOCUS       pCBL101       10631 bp DNA     circular SYN 27-MAY-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 10, 2023 from SnapGene Viewer 6.2.1
            https://www.snapgene.com
COMMENT     ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..10631
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_signal     1..25
                     /label=RB
                     /note="/vntifkey=87"
     primer_bind     333..377
                     /label=PZmUbi-F2
     primer_bind     358..377
                     /label=PzmUbi-F1
     promoter        392..2382
                     /label=PZmUbi
                     /label=UBI1ZM PRO
                     /note="Maize ubiquitin promoter"
                     /db_xref="SVFEATURE:F000004"
                     /db_xref="SVROLE:promoter"
     5'UTR           1288..1369
                     /label=UBI1ZM 5UTR (PHI)
                     /note="/db_xref=""SVFEATURE:F000015"
                     /db_xref="SVROLE:five_Prime_UTR"
     intron          1370..2382
                     /label=UBI1ZM INTRON1 (PHI)
                     /note="/db_xref=""SVFEATURE:F000030"
                     /db_xref="SVROLE:intron"
     primer_bind     complement(2377..2394)
                     /label=PZmUbi-F2
     primer_bind     2405..2426
                     /label=nptII-seq-F1
     misc_feature    2411..2458
                     /label=FRT1
                     /note="/db_xref=""SVFEATURE:F000005"
                     /db_xref="SVROLE:sequence_feature"
     CDS             2478..3272
                     /codon_start=1
                     /label=NPTII
                     /note="/db_xref=""SVFEATURE:F000457"
                     /db_xref="SVROLE:CDS"
                     /translation="MVEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     terminator      3291..3600
                     /label=PINII TERM
                     /note="Potato PINII terminator"
                     /db_xref="SVFEATURE:F000002"
                     /db_xref="SVROLE:terminator"
     primer_bind     3598..3608
                     /label=TpinII-R1
     primer_bind     complement(3599..3617)
                     /label=TpinII-R1
     terminator      3618..3830
                     /label=Tvsp
                     /note="/vntifkey=43"
     GC_signal       4014..4069
                     /label=LB
                     /note="/vntifkey=10"
     GC_signal       4070..4334
                     /label=LB
                     /note="/vntifkey=10"
     misc_signal     4070..4095
                     /label=LB
                     /label=L25
                     /note="/vntifkey=87"
     primer_bind     complement(4350..4363)
                     /label=LB-R1
     CDS             4396..5406
                     /codon_start=3
                     /label=SmR
                     /label=aadA\(spec)
                     /note="/vntifkey=4"
                     /translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQAR
                     YAVGRCLMLWSSNDVTQQGSRPKTKLNIMGEAVIAEVSTQLSEVVGVIERHLEPTLLAV
                     HLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTI
                     VVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPA
                     AEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVA
                     ADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    5652..6566
                     /label=pBR322
                     /note="/vntifkey=21"
     misc_feature    6910..9301
                     /label=pVS1
                     /note="/vntifkey=21"
     rep_origin      6910..7104
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7170..8243)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(8672..9301)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     primer_bind     10538..10557
                     /label=RB-F1
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
       61 aatctgatcc aagctcaagc tccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
      121 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
      181 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
      241 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgacatg
      301 attacgaatt cgagctcggt acccggggat cctctagagt cgacctgcag gcatgcaata
      361 gttccggact taccggcgcc gctagcctgc agtgcagcgt gacccggtcg tgcccctctc
      421 tagagataat gagcattgca tgtctaagtt ataaaaaatt accacatatt ttttttgtca
      481 cacttgtttg aagtgcagtt tatctatctt tatacatata tttaaacttt actctacgaa
      541 taatataatc tatagtacta caataatatc agtgttttag agaatcatat aaatgaacag
      601 ttagacatgg tctaaaggac aattgagtat tttgacaaca ggactctaca gttttatctt
      661 tttagtgtgc atgtgttctc cttttttttt gcaaatagct tcacctatat aatacttcat
      721 ccattttatt agtacatcca tttagggttt agggttaatg gtttttatag actaattttt
      781 ttagtacatc tattttattc tattttagcc tctaaattaa gaaaactaaa actctatttt
      841 agttttttta tttaataatt tagatataaa atagaataaa ataaagtgac taaaaattaa
      901 acaaataccc tttaagaaat taaaaaaact aaggaaacat ttttcttgtt tcgagtagat
      961 aatgccagcc tgttaaacgc cgtcgacgag tctaacggac accaaccagc gaaccagcag
     1021 cgtcgcgtcg ggccaagcga agcagacggc acggcatctc tgtcgctgcc tctggacccc
     1081 tctcgagagt tccgctccac cgttggactt gctccgctgt cggcatccag aaattgcgtg
     1141 gcggagcggc agacgtgagc cggcacggca ggcggcctcc tcctcctctc acggcaccgg
     1201 cagctacggg ggattccttt cccaccgctc cttcgctttc ccttcctcgc ccgccgtaat
     1261 aaatagacac cccctccaca ccctctttcc ccaacctcgt gttgttcgga gcgcacacac
     1321 acacaaccag atctccccca aatccacccg tcggcacctc cgcttcaagg tacgccgctc
     1381 gtcctccccc ccccccctct ctaccttctc tagatcggcg ttccggtcca tgcatggtta
     1441 gggcccggta gttctacttc tgttcatgtt tgtgttagat ccgtgtttgt gttagatccg
     1501 tgctgctagc gttcgtacac ggatgcgacc tgtacgtcag acacgttctg attgctaact
     1561 tgccagtgtt tctctttggg gaatcctggg atggctctag ccgttccgca gacgggatcg
     1621 atttcatgat tttttttgtt tcgttgcata gggtttggtt tgcccttttc ctttatttca
     1681 atatatgccg tgcacttgtt tgtcgggtca tcttttcatg cttttttttg tcttggttgt
     1741 gatgatgtgg tctggttggg cggtcgttct agatcggagt agaattctgt ttcaaactac
     1801 ctggtggatt tattaatttt ggatctgtat gtgtgtgcca tacatattca tagttacgaa
     1861 ttgaagatga tggatggaaa tatcgatcta ggataggtat acatgttgat gcgggtttta
     1921 ctgatgcata tacagagatg ctttttgttc gcttggttgt gatgatgtgg tgtggttggg
     1981 cggtcgttca ttcgttctag atcggagtag aatactgttt caaactacct ggtgtattta
     2041 ttaattttgg aactgtatgt gtgtgtcata catcttcata gttacgagtt taagatggat
     2101 ggaaatatcg atctaggata ggtatacatg ttgatgtggg ttttactgat gcatatacat
     2161 gatggcatat gcagcatcta ttcatatgct ctaaccttga gtacctatct attataataa
     2221 acaagtatgt tttataatta ttttgatctt gatatacttg gatgatggca tatgcagcag
     2281 ctatatgtgg atttttttag ccctgccttc atacgctatt tatttgcttg gtactgtttc
     2341 ttttgtcgat gctcaccctg ttgtttggtg ttacttctgc aggtcgactc tagaggatca
     2401 attcgctagc gaagttccta ttccgaagtt cctattctct agaaagtata ggaacttcag
     2461 atccaccggg atccaccatg gttgaacaag atggattgca cgcaggttct ccggccgctt
     2521 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     2581 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     2641 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     2701 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     2761 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     2821 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     2881 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     2941 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     3001 aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga
     3061 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     3121 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     3181 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     3241 ccttctatcg ccttcttgac gagttcttct gaggatccac catggttaac ctagacttgt
     3301 ccatcttctg gattggccaa cttaattaat gtatgaaata aaaggatgca cacatagtga
     3361 catgctaatc actataatgt gggcatcaaa gttgtgtgtt atgtgtaatt actagttatc
     3421 tgaataaaag agaaagagat catccatatt tcttatccta aatgaatgtc acgtgtcttt
     3481 ataattcttt gatgaaccag atgcatttca ttaaccaaat ccatatacat ataaatatta
     3541 atcatatata attaatatca attgggttag caaaacaaat ctagtctagg tgtgttttgc
     3601 aagcttgcat gcctgcagtc gtctattgga ccgacagttg ctattaatat aatgggccac
     3661 catagtagac tgacaaataa attacctgac aacatcgttt cacaaaaaaa caaacacaaa
     3721 aagggagtgc attttccagg gcatttttgt aataaaaaac agttaaaagg gagtgcaata
     3781 gaaatatagg ggtgtggaaa tagtgatttg agcacgtctt gaagcgaatt agcttggcac
     3841 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     3901 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     3961 cttcccaaca gttgcgcagc ctgaatggcg aatgctagag caattcggcg ttaattcagt
     4021 acattaaaaa cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg tttacaccac
     4081 aatatatcct gccaccagcc agccaacagc tccccgaccg gcagctcggc acaaaatcac
     4141 cactcgatac aggcagccca tcagtccggg acggcgtcag cgggagagcc gttgtaaggc
     4201 ggcagacttt gctcatgtta ccgatgctat tcggaagaac ggcaactaag ctgccgggtt
     4261 tgaaacacgg atgatctcgc ggagggtagc atgttgattg taacgatgac agagcgttgc
     4321 tgcctgtgat caattcgggc acgaacccag tggacataag cctgttcggt tcgtaagctg
     4381 taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt
     4441 ggtaacggcg cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct
     4501 atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg
     4561 agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa catcatgggg
     4621 gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc
     4681 catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg
     4741 aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg
     4801 cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt
     4861 ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca
     4921 gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc
     4981 gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc
     5041 gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta
     5101 tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat
     5161 gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc
     5221 gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc
     5281 gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc
     5341 gcagatcagt tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc
     5401 aaataatgtc tagctagaaa ttcgttcaag ccgacgccgc ttcgcggcgc ggcttaactc
     5461 aagcgttaga tgcactaagc acataattgc tcacagccaa actatcaggt caagtctgct
     5521 tttattattt ttaagcgtgc ataataagcc ctacacaaat tgggagatat atcatgcatg
     5581 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     5641 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     5701 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     5761 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     5821 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     5881 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     5941 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     6001 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     6061 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     6121 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     6181 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     6241 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     6301 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     6361 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     6421 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg
     6481 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat
     6541 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
     6601 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     6661 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagggt gccttgatgt
     6721 gggcgccggc ggtcgagtgg cgacggcgcg gcttgtccgc gccctggtag attgcctggc
     6781 cgtaggccag ccatttttga gcggccagcg gccgcgatag gccgacgcga agcggcgggg
     6841 cgtagggagc gcagcgaccg aagggtaggc gctttttgca gctcttcggc tgtgcgctgg
     6901 ccagacagtt atgcacaggc caggcgggtt ttaagagttt taataagttt taaagagttt
     6961 taggcggaaa aatcgccttt tttctctttt atatcagtca cttacatgtg tgaccggttc
     7021 ccaatgtacg gctttgggtt cccaatgtac gggttccggt tcccaatgta cggctttggg
     7081 ttcccaatgt acgtgctatc cacaggaaag agaccttttc gacctttttc ccctgctagg
     7141 gcaatttgcc ctagcatctg ctccgtacat taggaaccgg cggatgcttc gccctcgatc
     7201 aggttgcggt agcgcatgac taggatcggg ccagcctgcc ccgcctcctc cttcaaatcg
     7261 tactccggca ggtcatttga cccgatcagc ttgcgcacgg tgaaacagaa cttcttgaac
     7321 tctccggcgc tgccactgcg ttcgtagatc gtcttgaaca accatctggc ttctgccttg
     7381 cctgcggcgc ggcgtgccag gcggtagaga aaacggccga tgccgggatc gatcaaaaag
     7441 taatcggggt gaaccgtcag cacgtccggg ttcttgcctt ctgtgatctc gcggtacatc
     7501 caatcagcta gctcgatctc gatgtactcc ggccgcccgg tttcgctctt tacgatcttg
     7561 tagcggctaa tcaaggcttc accctcggat accgtcacca ggcggccgtt cttggccttc
     7621 ttcgtacgct gcatggcaac gtgcgtggtg tttaaccgaa tgcaggtttc taccaggtcg
     7681 tctttctgct ttccgccatc ggctcgccgg cagaacttga gtacgtccgc aacgtgtgga
     7741 cggaacacgc ggccgggctt gtctcccttc ccttcccggt atcggttcat ggattcggtt
     7801 agatgggaaa ccgccatcag taccaggtcg taatcccaca cactggccat gccggccggc
     7861 cctgcggaaa cctctacgtg cccgtctgga agctcgtagc ggatcacctc gccagctcgt
     7921 cggtcacgct tcgacagacg gaaaacggcc acgtccatga tgctgcgact atcgcgggtg
     7981 cccacgtcat agagcatcgg aacgaaaaaa tctggttgct cgtcgccctt gggcggcttc
     8041 ctaatcgacg gcgcaccggc tgccggcggt tgccgggatt ctttgcggat tcgatcagcg
     8101 gccgcttgcc acgattcacc ggggcgtgct tctgcctcga tgcgttgccg ctgggcggcc
     8161 tgcgcggcct tcaacttctc caccaggtca tcacccagcg ccgcgccgat ttgtaccggg
     8221 ccggatggtt tgcgaccgct cacgccgatt cctcgggctt gggggttcca gtgccattgc
     8281 agggccggca gacaacccag ccgcttacgc ctggccaacc gcccgttcct ccacacatgg
     8341 ggcattccac ggcgtcggtg cctggttgtt cttgattttc catgccgcct cctttagccg
     8401 ctaaaattca tctactcatt tattcatttg ctcatttact ctggtagctg cgcgatgtat
     8461 tcagatagca gctcggtaat ggtcttgcct tggcgtaccg cgtacatctt cagcttggtg
     8521 tgatcctccg ccggcaactg aaagttgacc cgcttcatgg ctggcgtgtc tgccaggctg
     8581 gccaacgttg cagccttgct gctgcgtgcg ctcggacggc cggcacttag cgtgtttgtg
     8641 cttttgctca ttttctcttt acctcattaa ctcaaatgag ttttgattta atttcagcgg
     8701 ccagcgcctg gacctcgcgg gcagcgtcgc cctcgggttc tgattcaaga acggttgtgc
     8761 cggcggcggc agtgcctggg tagctcacgc gctgcgtgat acgggactca agaatgggca
     8821 gctcgtaccc ggccagcgcc tcggcaacct caccgccgat gcgcgtgcct ttgatcgccc
     8881 gcgacacgac aaaggccgct tgtagccttc catccgtgac ctcaatgcgc tgcttaacca
     8941 gctccaccag gtcggcggtg gcccatatgt cgtaagggct tggctgcacc ggaatcagca
     9001 cgaagtcggc tgccttgatc gcggacacag ccaagtccgc cgcctggggc gctccgtcga
     9061 tcactacgaa gtcgcgccgg ccgatggcct tcacgtcgcg gtcaatcgtc gggcggtcga
     9121 tgccgacaac ggttagcggt tgatcttccc gcacggccgc ccaatcgcgg gcactgccct
     9181 ggggatcgga atcgactaac agaacatcgg ccccggcgag ttgcagggcg cgggctagat
     9241 gggttgcgat ggtcgtcttg cctgacccgc ctttctggtt aagtacagcg ataaccttca
     9301 tgcgttcccc ttgcgtattt gtttatttac tcatcgcatc atatacgcag cgaccgcatg
     9361 acgcaagctg ttttactcaa atacacatca cctttttaga cggcggcgct cggtttcttc
     9421 agcggccaag ctggccggcc aggccgccag cttggcatca gacaaaccgg ccaggatttc
     9481 atgcagccgc acggttgaga cgtgcgcggg cggctcgaac acgtacccgg ccgcgatcat
     9541 ctccgcctcg atctcttcgg taatgaaaaa cggttcgtcc tggccgtcct ggtgcggttt
     9601 catgcttgtt cctcttggcg ttcattctcg gcggccgcca gggcgtcggc ctcggtcaat
     9661 gcgtcctcac ggaaggcacc gcgccgcctg gcctcggtgg gcgtcacttc ctcgctgcgc
     9721 tcaagtgcgc ggtacagggt cgagcgatgc acgccaagca gtgcagccgc ctctttcacg
     9781 gtgcggcctt cctggtcgat cagctcgcgg gcgtgcgcga tctgtgccgg ggtgagggta
     9841 gggcgggggc caaacttcac gcctcgggcc ttggcggcct cgcgcccgct ccgggtgcgg
     9901 tcgatgatta gggaacgctc gaactcggca atgccggcga acacggtcaa caccatgcgg
     9961 ccggccggcg tggtggtgtc ggcccacggc tctgccaggc tacgcaggcc cgcgccggcc
    10021 tcctggatgc gctcggcaat gtccagtagg tcgcgggtgc tgcgggccag gcggtctagc
    10081 ctggtcactg tcacaacgtc gccagggcgt aggtggtcaa gcatcctggc cagctccggg
    10141 cggtcgcgcc tggtgccggt gatcttctcg gaaaacagct tggtgcagcc ggccgcgtgc
    10201 agttcggccc gttggttggt caagtcctgg tcgtcggtgc tgacgcgggc atagcccagc
    10261 aggccagcgg cggcgctctt gttcatggcg taatgtctcc ggttctagtc gcaagtattc
    10321 tactttatgc gactaaaaca cgcgacaaga aaacgccagg aaaagggcag ggcggcagcc
    10381 tgtcgcgtaa cttaggactt gtgcgacatg tcgttttcag aagacggctg cactgaacgt
    10441 cagaagccga ctgcactata gcagcggagg ggttggatca aagtacttta aagtacttta
    10501 aagtacttta aagtactttg atcccgaggg gaaccctgtg gttggcatgc acatacaaat
    10561 ggacgaacgg ataaaccttt tcacgccctt ttaaatatcc gattattcta ataaacgctc
    10621 ttttctctta g
//
