LOCUS       CN3781_pAAV-eHGT_452h-minBglobin-iCre(R297T)-BGHpA        4881 bp    DNA     circular     19-MAR-1980
DEFINITION  .
ACCESSION   urn.local...iv-e16pvjk
SOURCE      
  ORGANISM  .
COMMENT     ##Author-START##
            Name :: Jonathan Ting
            ##Author-END##
            ##Imported_From-START##
            Path     :: \\allen\programs\celltypes\workgroups\0285\CN-VectorNTI
            Filename :: CN3084 (JT).gb
            ##Imported_From-END##
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
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COMMENT     LSOWNER|
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FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /vntifkey="21"
                     /label=L-ITR
     misc_feature    157..741
                     /created_by="ximena.opitzaraya"
                     /vntifkey="21"
                     /label=eHGT_452h
     misc_feature    748..800
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=Beta\Globin\minimal\promoter
     misc_feature    829..1884
                     /vntifkey="21"
                     /label=iCre(297T)
     misc_feature    829..855
                     /vntifkey="21"
                     /label=nls
     misc_feature    1741..1743
                     /vntifkey="21"
                     /label=R297T
     misc_feature    1872
                     /vntifkey="21"
                     /label=G-->A\silent\mutation\to\remove\XhoI\site
     misc_feature    1918..2121
                     /vntifkey="21"
                     /label=BGH-pA
     misc_feature    2144..2284
                     /vntifkey="21"
                     /label=R-ITR
     rep_origin      2376..2682
                     /vntifkey="33"
                     /label=f1\Origin
     misc_feature    3201..4058
                     /vntifkey="21"
                     /label=AmpR
     rep_origin      4209..4876
                     /vntifkey="33"
                     /label=pUC\ori
BASE COUNT     1150 a      1282 c      1275 g      1174 t 
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 
      121 actccatcac taggggttcc tgcggccgca cgcgtaggga ggaactaaag tccattcata 
      181 tgtccatatt ctcaatttgg gaaaaaatgt ggctttgtct tcatcctctt ttcctgagtg 
      241 tataaggcca gaagccaaag gcaagagcag gacattccag gagcctttat gattgctcta 
      301 agtcacatct gctctcgagc tcccattcca ctgtgaaaaa tacctagtct tgaactttgg 
      361 agttccaaat tccacatgat cgacttcctc aaggcttttc acttgacgaa caaattgcat 
      421 gccaagaaat cgtcaaaatt ctatcaagct taattatgca atttctgtct ataaatgaca 
      481 tacctcctgt accctatgac acccacctat gcactaataa gatgtgacag ctgcatcacc 
      541 aacaaagcct cccccgcctg ccattctctt cggaggtgag cacctcctgc tcgggaggaa 
      601 ctgacagccc ctttcagaaa tgtgaaggca gtgaagtccc aaaccagaaa cagtgtgacc 
      661 acctaggctc acccaaggac cgtcctgtgc ttctgggcaa ccaatggatg tagggggtta 
      721 ggaggcagtg gggattcgtc agagctcggg ctgggcataa aagtcagggc agagccatct 
      781 attgcttaca tttgcttctg ggatccagat ctttccaagc tagccaccat ggtgcccaag 
      841 aagaagagga aagtctccaa cctgctgact gtgcaccaaa acctgcctgc cctccctgtg 
      901 gatgccacct ctgatgaagt caggaagaac ctgatggaca tgttcaggga caggcaggcc 
      961 ttctctgaac acacctggaa gatgctcctg tctgtgtgca gatcctgggc tgcctggtgc 
     1021 aagctgaaca acaggaaatg gttccctgct gaacctgagg atgtgaggga ctacctcctg 
     1081 tacctgcaag ccagaggcct ggctgtgaag accatccaac agcacctggg ccagctcaac 
     1141 atgctgcaca ggagatctgg cctgcctcgc ccttctgact ccaatgctgt gtccctggtg 
     1201 atgaggagaa tcagaaagga gaatgtggat gctggggaga gagccaagca ggccctggcc 
     1261 tttgaacgca ctgactttga ccaagtcaga tccctgatgg agaactctga cagatgccag 
     1321 gacatcagga acctggcctt cctgggcatt gcctacaaca ccctgctgcg cattgccgaa 
     1381 attgccagaa tcagagtgaa ggacatctcc cgcaccgatg gtgggagaat gctgatccac 
     1441 attggcagga ccaagaccct ggtgtccaca gctggtgtgg agaaggccct gtccctgggg 
     1501 gttaccaagc tggtggagag atggatctct gtgtctggtg tggctgatga ccccaacaac 
     1561 tacctgttct gccgggtcag aaagaatggt gtggctgccc cttctgccac ctcccaactg 
     1621 tccacccggg ccctggaagg gatctttgag gccacccacc gcctgatcta tggtgccaag 
     1681 gatgactctg ggcagagata cctggcctgg tctggccact ctgccagagt gggtgctgcc 
     1741 accgacatgg ccagggctgg tgtgtccatc cctgaaatca tgcaggctgg tggctggacc 
     1801 aatgtgaaca ttgtgatgaa ctacatcaga aacctggact ctgagactgg ggccatggtg 
     1861 aggctgctcg aagatgggga ctgaggcgcg ccgaattcaa gcttctcgag agatcttcga 
     1921 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc 
     1981 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc 
     2041 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt 
     2101 gggaagacaa tagcaggcat gcacgtgcgg accgagcggc cgcaggaacc cctagtgatg 
     2161 gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc 
     2221 gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg 
     2281 caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata 
     2341 cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg 
     2401 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct 
     2461 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc 
     2521 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg 
     2581 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt 
     2641 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg 
     2701 gctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc 
     2761 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt 
     2821 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa 
     2881 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg 
     2941 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga 
     3001 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 
     3061 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 
     3121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 
     3181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 
     3241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 
     3301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 
     3361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 
     3421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac 
     3481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 
     3541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 
     3601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 
     3661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 
     3721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg 
     3781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 
     3841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 
     3901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct 
     3961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac 
     4021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 
     4081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 
     4141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 
     4201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 
     4261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 
     4321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc 
     4381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 
     4441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 
     4501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 
     4561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 
     4621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 
     4681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 
     4741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 
     4801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 
     4861 gctggccttt tgctcacatg t 
//
