LOCUS mId1_GFP 5167 bp DNA circular 11-APR-2020 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4733) AUTHORS . TITLE Direct Submission JOURNAL Exported Thursday, Dec 26, 2019 from SnapGene Viewer 5.0.7 https://www.snapgene.com COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers source 1..635 /organism="synthetic DNA construct" /mol_type="other DNA" /locus_tag="source:synthetic DNA construct" /label="source:synthetic DNA construct" /ApEinfo_label="source:synthetic DNA construct" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" enhancer 61..364 /note="human cytomegalovirus immediate early enhancer" /locus_tag="CMV enhancer" /label="CMV enhancer" /ApEinfo_label="CMV enhancer" /ApEinfo_fwdcolor="#5ac3fa" /ApEinfo_revcolor="#5ac3fa" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" promoter 365..568 /note="human cytomegalovirus (CMV) immediate early promoter" /locus_tag="CMV promoter" /label="CMV promoter" /ApEinfo_label="CMV promoter" /ApEinfo_fwdcolor="#346ee0" /ApEinfo_revcolor="#346ee0" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" misc_feature 591..635 /note="multiple cloning site" /locus_tag="MCS" /label="MCS" /ApEinfo_label="MCS" /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" regulatory 1107..1116 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /locus_tag="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /ApEinfo_label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" CDS 1113..1829 /codon_start=1 /product="enhanced GFP" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT L KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKD D GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGI K VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVL L EFVTAAGITLGMDELYK" /locus_tag="EGFP" /label="EGFP" /ApEinfo_label="EGFP" /ApEinfo_fwdcolor="#e9d024" /ApEinfo_revcolor="#e9d024" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" polyA_signal 1955..2076 /note="SV40 polyadenylation signal" /locus_tag="SV40 poly(A) signal" /label="SV40 poly(A) signal" /ApEinfo_label="SV40 poly(A) signal" /ApEinfo_fwdcolor="#ff3eee" /ApEinfo_revcolor="#ff3eee" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" rep_origin complement(2083..2538) /direction=LEFT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /locus_tag="f1 ori" /label="f1 ori" /ApEinfo_label="f1 ori" /ApEinfo_fwdcolor="#999999" /ApEinfo_revcolor="#999999" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" promoter 2565..2669 /gene="bla" /locus_tag="AmpR promoter" /label="AmpR promoter" /ApEinfo_label="AmpR promoter" /ApEinfo_fwdcolor="#346ee0" /ApEinfo_revcolor="#346ee0" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" promoter 2671..3028 /note="SV40 enhancer and early promoter" /locus_tag="SV40 promoter" /label="SV40 promoter" /ApEinfo_label="SV40 promoter" /ApEinfo_fwdcolor="#346ee0" /ApEinfo_revcolor="#346ee0" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" rep_origin 2879..3014 /note="SV40 origin of replication" /locus_tag="SV40 ori" /label="SV40 ori" /ApEinfo_label="SV40 ori" /ApEinfo_fwdcolor="#999999" /ApEinfo_revcolor="#999999" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" CDS 3063..3857 /codon_start=1 /product="aminoglycoside phosphotransferase" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR P VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL S SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH Q GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI A LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" /locus_tag="NeoR/KanR" /label="NeoR/KanR" /ApEinfo_label="NeoR/KanR" /ApEinfo_fwdcolor="#e9d024" /ApEinfo_revcolor="#e9d024" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" polyA_signal 4089..4136 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /locus_tag="HSV TK poly(A) signal" /label="HSV TK poly(A) signal" /ApEinfo_label="HSV TK poly(A) signal" /ApEinfo_fwdcolor="#ff3eee" /ApEinfo_revcolor="#ff3eee" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" rep_origin 4465..5053 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /locus_tag="ori" /label="ori" /ApEinfo_label="ori" /ApEinfo_fwdcolor="#999999" /ApEinfo_revcolor="#999999" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" source 1079..5167 /organism="synthetic DNA construct" /mol_type="other DNA" /locus_tag="source:synthetic DNA construct(1)" /label="source:synthetic DNA construct(1)" /ApEinfo_label="source:synthetic DNA construct" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" misc_feature 1079..1105 /note="multiple cloning site" /locus_tag="MCS(1)" /label="MCS(1)" /ApEinfo_label="MCS" /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" misc_feature 636..1078 /locus_tag="Mouse Id1" /label="Mouse Id1" /ApEinfo_label="Mouse Id1" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgagct caagcttcga attctatgaa ggtcgccagt ggcagtgccg 661 cagccgctgc aggccctagc tgttcgctga aggcgggcag gacagcgggc gaggtggtac 721 ttggtctgtc ggagcaaagc gtggccatct cgcgctgcgc tgggacgcgc ctgcccgcct 781 tgctggacga gcagcaggtg aacgtcctgc tctacgacat gaacggctgc tactcacgcc 841 tcaaggagct ggtgcccacc ctgccccaga accgcaaagt gagcaaggtg gagatcctgc 901 agcatgtaat cgactacatc agggacctgc agctggagct gaactcggag tctgaagtcg 961 ggaccaccgg aggccgggga ctgcctgtcc gcgccccgct cagcaccctg aacggcgaga 1021 tcagtgcctt ggcggccgag gcggcatgtg ttccagccga cgatcgcatc ttgtgtcggg 1081 taccgcgggc ccgggatcca ccggtcgcca ccatggtgag caagggcgag gagctgttca 1141 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg 1201 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca 1261 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc 1321 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc 1381 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc 1441 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg 1501 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca 1561 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc 1621 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg 1681 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca 1741 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga 1801 tcactctcgg catggacgag ctgtacaagt aaagcggccg cgactctaga tcataatcag 1861 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa 1921 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg 1981 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 2041 tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta agcgttaata 2101 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg 2161 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc 2221 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa 2281 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt 2341 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac 2401 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta 2461 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg 2521 cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt 2581 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa 2641 tgcttcaata atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt 2701 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat 2761 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag 2821 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat 2881 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt 2941 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg 3001 cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc 3061 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat 3121 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt 3181 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac 3241 tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg 3301 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc 3361 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa 3421 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc 3481 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg 3541 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg 3601 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa 3661 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg 3721 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct 3781 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc 3841 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa 3901 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat 3961 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt 4021 cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg 4081 cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa 4141 acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg 4201 ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc 4261 agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat 4321 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt 4381 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc 4441 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt 4501 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac 4561 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt 4621 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct 4681 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga 4741 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac 4801 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg 4861 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt 4921 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc 4981 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg 5041 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc 5101 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc 5161 catgcat //