LOCUS       LentiCROP-PuroR-P2A-GFP 9078 bp ds-DNA     circular     16-MAR-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      complement(502..1117)
                     /label="pUC Ori"
     LTR             1465..1877
                     /label="5' LTR"
     misc_feature    1928..2065
                     /label="psi"
     misc_feature    2032..2396
                     /label="gag"
     misc_feature    2542..2783
                     /label="RRE"
     misc_feature    3184..3301
                     /label="cPPT"
     misc_feature    3353..3354
                     /label="origin"
     promoter        3360..4618
                     /label="EF-1a"
     CDS             4631..5227
                     /label="Puro"
     misc_feature    5228..5293
                     /label="P2A"
     CDS             5294..6010
                     /label="eGFP"
     misc_feature    5341..5364
                     /label="4r"
     misc_feature    5936..5959
                     /label="GPS3f"
     misc_RNA        6029..6617
                     /label="WPRE"
     LTR             6688..6723
                     /label="3' LTR"
     promoter        6773..7017
                     /label="hU6"
     LTR             7094..7293
                     /label="3' LTR"
     polyA_signal    7370..7500
                     /label="SV40 polyA"
     CDS             8648..376
                     /label="AmpR"
ORIGIN
        1 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
       61 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
      121 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
      181 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
      241 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
      301 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
      361 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
      421 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
      481 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
      541 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
      601 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
      661 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
      721 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
      781 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
      841 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
      901 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
      961 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     1021 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     1081 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     1141 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     1201 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     1261 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     1321 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     1381 tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag
     1441 ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc
     1501 aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc
     1561 cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg
     1621 acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta
     1681 gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     1741 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     1801 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     1861 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
     1921 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
     1981 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
     2041 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
     2101 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
     2161 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
     2221 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
     2281 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
     2341 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc
     2401 agacctggag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta
     2461 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
     2521 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
     2581 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
     2641 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
     2701 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
     2761 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
     2821 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
     2881 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
     2941 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
     3001 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
     3061 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
     3121 aggcagggat attcaccatt atcgtttcag aatcttgaga caaatggcag tattcatcca
     3181 caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     3241 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     3301 tcgggtttat tacagggaca gcagagatcc actttggcgc cggctcgagg gggcccgggt
     3361 gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt
     3421 cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac
     3481 agtccccgag aagttggggg gaggggtcgg caattgatcc ggtgcctaga gaaggtggcg
     3541 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
     3601 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
     3661 cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg
     3721 cccttgcgtg ccttgaatta cttccacctg gctgcagtac gtgattcttg atcccgagct
     3781 tcgggttgga agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg
     3841 tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct
     3901 tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc
     3961 tgcgacgctt tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg
     4021 tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc
     4081 ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg
     4141 ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag
     4201 gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc
     4261 agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca
     4321 aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc
     4381 gccgtccagg cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg
     4441 ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc
     4501 agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt
     4561 cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgacg
     4621 tacggccacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     4681 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     4741 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     4801 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgccgtgg cggtctggac
     4861 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     4921 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     4981 gcccaaggag cccgcgtggt tcctggccac cgtcggagtc tcgcccgacc accagggcaa
     5041 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     5101 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     5161 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     5221 cggtgccGGA AGCGGAGCTA CTAACTTCAG CCTGCTGAAG CAGGCTGGCG ACGTGGAGGA
     5281 GAACCCTGGA CCTATGGTGA GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG TGCCCATCCT
     5341 GGTCGAGCTG GACGGCGACG TAAACGGCCA CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG
     5401 CGATGCCACC TACGGCAAGC TGACCCTGAA GTTCATCTGC ACCACCGGCA AGCTGCCCGT
     5461 GCCCTGGCCC ACCCTCGTGA CCACCCTGAC CTACGGCGTG CAGTGCTTCA GCCGCTACCC
     5521 CGACCACATG AAGCAGCACG ACTTCTTCAA GTCCGCCATG CCCGAAGGCT ACGTCCAGGA
     5581 GCGCACCATC TTCTTCAAGG ACGACGGCAA CTACAAGACC CGCGCCGAGG TGAAGTTCGA
     5641 GGGCGACACC CTGGTGAACC GCATCGAGCT GAAGGGCATC GACTTCAAGG AGGACGGCAA
     5701 CATCCTGGGG CACAAGCTGG AGTACAACTA CAACAGCCAC AACGTCTATA TCATGGCCGA
     5761 CAAGCAGAAG AACGGCATCA AGGTGAACTT CAAGATCCGC CACAACATCG AGGACGGCAG
     5821 CGTGCAGCTC GCCGACCACT ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT
     5881 GCCCGACAAC CACTACCTGA GCACCCAGTC CGCCCTGAGC AAAGACCCCA ACGAGAAGCG
     5941 CGATCACATG GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG ATCACTCTCG GCATGGACGA
     6001 GCTGTACAAG TGAacgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag
     6061 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     6121 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     6181 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     6241 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     6301 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     6361 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     6421 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     6481 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     6541 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     6601 ttgggccgcc tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt
     6661 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     6721 gatttcacca ttatcgtttc agacccacct cccaaccccg aggggaccca gagagggcct
     6781 atttcccatg attccttcat atttgcatat acgatacaag gctgttagag agataattag
     6841 aattaatttg actgtaaaca caaagatatt agtacaaaat acgtgacgta gaaagtaata
     6901 atttcttggg tagtttgcag ttttaaaatt atgttttaaa atggactatc atatgcttac
     6961 cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgaaacac
     7021 cgGAGACGGT TGTAAATTTG AGACATTGGT GGACGCGTCG TCTCAaagct tggcgtaact
     7081 agatcttgag acactgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     7141 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     7201 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     7261 acccttttag tcagtgtgga aaatctctag cagtacgtat agtagttcat gtcatcttat
     7321 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     7381 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     7441 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     7501 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     7561 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     7621 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca
     7681 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg
     7741 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     7801 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     7861 atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     7921 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     7981 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     8041 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     8101 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     8161 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     8221 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     8281 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
     8341 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     8401 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     8461 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     8521 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     8581 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     8641 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     8701 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     8761 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     8821 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     8881 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     8941 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     9001 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     9061 ccggcaacaa ttaataga
//