LOCUS       piggyBAC-U6-GFP-WPRE    7969 bp ds-DNA     circular     16-MAR-2023
DEFINITION  .
COMMENT     Imported using the Genbank importer. File name:
            PiggyBac-CMV-MCS-EF1-Puro_PB510B-1.ape ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     812..831
                     /label="BII(R)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    870..873
                     /label="TTAA"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    874..886
                     /label="13 bp terminal repeat (TR)"
                     /ApEinfo_revcolor="#cd0532"
                     /ApEinfo_fwdcolor="#cd0532"
     misc_feature    874..936
                     /label="5'PB Terminal Repeat"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     misc_feature    887..917
                     /label="31 bp spacer"
                     /ApEinfo_revcolor="#0080c0"
                     /ApEinfo_fwdcolor="#0080c0"
     misc_feature    918..936
                     /label="19 bp Internal Repeat"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1112..1343
                     /label="core Insulator"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          1358..1383
                     /label="046_choi_pe_LG_BsmbI_fwd"
                     /note="sequence: agagagggcctaTTTCCCATGATTCC"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     promoter        1370..1609
                     /label="hU6_promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          1532..1551
                     /label="LKO1_5_primer"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     promoter        1712..2970
                     /label="EF-1a"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             2983..3699
                     /label="eGFP"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    3625..3648
                     /label="GPS3f"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(3763..3784)
                     /label="10X_seq1"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_RNA        3819..4387
                     /label="WPRE"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    4539..4669
                     /label="SV40 polyA"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    4918..5148
                     /label="core Insulator"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_fwdcolor="#ff0080"
     misc_feature    5423..5441
                     /label="19 bp Internal Repeat(IR)"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_fwdcolor="#ff0080"
     misc_feature    5423..5457
                     /label="3' PB Terminal Repeat"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    5442..5444
                     /label="3 bp spacer"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    5445..5457
                     /label="13 bp Terminal Repeat (TR)"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    5458..5461
                     /label="TTAA"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    complement(5664..5683)
                     /label="Potential primer mismatch"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer_bind     complement(5976..5995)
                     /label="pLac(F)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    6350..6938
                     /label="ORI (mismatch with STAR by 1)"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             complement(7112..7969)
                     /label="Amp"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
ORIGIN
        1 ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA
       61 CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA
      121 AGTGCCACCT AAATTGTAAG CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA
      181 ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA
      241 TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC
      301 GTGGACTCCA ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA
      361 CCATCACCCT AATCAAGTTT TTTGGGGTCG AGGTGCCGTA AAGCACTAAA TCGGAACCCT
      421 AAAGGGAGCC CCCGATTTAG AGCTTGACGG GGAAAGCCGG CGAACGTGGC GAGAAAGGAA
      481 GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT CACGCTGCGC
      541 GTAACCACCA CACCCGCCGC GCTTAATGCG CCGCTACAGG GCGCGTCCCA TTCGCCATTC
      601 AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG
      661 GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA
      721 CGACGTTGTA AAACGACGGC CAGTGAGCGC GCCTCGTTCA TTCACGTTTT TGAACCCGTG
      781 GAGGACGGGC AGACTCGCGG TGCAAATGTG TTTTACAGCG TGATGGAGCA GATGAAGATG
      841 CTCGACACGC TGCAGAACAC GCAGCTAGAT TAACCCTAGA AAGATAATCA TATTGTGACG
      901 TACGTTAAAG ATAATCATGT GTAAAATTGA CGCATGTGTT TTATCGGTCT GTATATCGAG
      961 GTTTATTTAT TAATTTGAAT AGATATTAAG TTTTATTATA TTTACACTTA CATACTAATA
     1021 ATAAATTCAA CAAACAATTT ATTTATGTTT ATTTATTTAT TAAAAAAAAC AAAAACTCAA
     1081 AATTTCTTCT ATAAAGTAAC AAAACTTTTA TGAGGGACAG CCCCCCCCCA AAGCCCCCAG
     1141 GGATGTAATT ACGTCCCTCC CCCGCTAGGG GGCAGCAGCG AGCCGCCCGG GGCTCCGCTC
     1201 CGGTCCGGCG CTCCCCCCGC ATCCCCGAGC CGGCAGCGTG CGGGGACAGC CCGGGCACGG
     1261 GGAAGGTGGC ACGGGATCGC TTTCCTCTGA ACGCTTCTCG CTGCTCTTTG AGCCTGCAGA
     1321 CACCTGGGGG GATACGGGGA AAAGGCCTCC ACGGCCAaga gagggcctaT TTCCCATGAT
     1381 TCCTTCATAT TTGCATATAC GATACAAGGC TGTTAGAGAG ATAATTAGAA TTAATTTGAC
     1441 TGTAAACACA AAGATATTAG TACAAAATAC GTGACGTAGA AAGTAATAAT TTCTTGGGTA
     1501 GTTTGCAGTT TTAAAATTAT GTTTTAAAAT GGACTATCAT ATGCTTACCG TAACTTGAAA
     1561 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAAACACCg GAGACGGTTG
     1621 TAAATTTGAG ACATTGGTGG ACGCGTCGTC TCAttttttt attacaggga cagcagagat
     1681 ccactttggc gccggctcga gggggcccgg gtgcaaagat ggataaagtt ttaaacagag
     1741 aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat tggctccggt
     1801 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc
     1861 ggcaattgat ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg
     1921 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc
     1981 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt
     2041 tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc
     2101 tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg
     2161 aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc
     2221 tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag
     2281 tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt
     2341 cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc
     2401 gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca
     2461 ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg
     2521 ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga
     2581 gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc
     2641 tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc
     2701 gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg
     2761 agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc
     2821 cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg
     2881 gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa
     2941 agtttttttc ttccatttca ggtgtcgtga cgtacggcca ccATGGTGAG CAAGGGCGAG
     3001 GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC
     3061 AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG
     3121 TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGACC
     3181 TACGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG
     3241 TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC
     3301 TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG
     3361 AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA GTACAACTAC
     3421 AACAGCCACA ACGTCTATAT CATGGCCGAC AAGCAGAAGA ACGGCATCAA GGTGAACTTC
     3481 AAGATCCGCC ACAACATCGA GGACGGCAGC GTGCAGCTCG CCGACCACTA CCAGCAGAAC
     3541 ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG CACCCAGTCC
     3601 GCCCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA GTTCGTGACC
     3661 GCCGCCGGGA TCACTCTCGG CATGGACGAG CTGTACAAGT GAagtcgact tTCCAAGCTC
     3721 CATAGGTCTG ATTTGGTACC ATAGCAGATG ATCCATGGTA CCGCTTTAAG GCCGGTCCTA
     3781 GCAAtcgcgt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     3841 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     3901 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     3961 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     4021 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     4081 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     4141 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     4201 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     4261 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     4321 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     4381 tccccgcgtc gactttaaga ccaatgactt acaaggcacg cgtAATCAAC CTCTGGATTA
     4441 CAAAATTTGT GAAAGATTGA CTGGTATTCT TAACTATGTT GCTCCTTTTA CGCTATGTGG
     4501 ATACGCTGCT TTAATGCCTT TGTATCATGC GTTAACTAAA CTTGTTTATT GCAGCTTATA
     4561 ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC
     4621 ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGG AATTGACTCA
     4681 AATGATGTCA ATTAGTCTAT CAGAAGCTCA TCTGGTCTCC CTTCCGGGGG ACAAGACATC
     4741 CCTGTTTAAT ATTTAAACAG CAGTGTTCCC AAACTGGGTT CTTATATCCC TTGCTCTGGT
     4801 CAACCAGGTT GCAGGGTTTC CTGTCCTCAC AGGAACGAAG TCCCTAAAGA AACAGTGGCA
     4861 GCCAGGTTTA GCCCCGGAAT TGACTGGATT CCTTTTTTAG GGCCCATTGG TATGGCTTTT
     4921 TCCCCGTATC CCCCCAGGTG TCTGCAGGCT CAAAGAGCAG CGAGAAGCGT TCAGAGGAAA
     4981 GCGATCCCGT GCCACCTTCC CCGTGCCCGG GCTGTCCCCG CACGCTGCCG GCTCGGGGAT
     5041 GCGGGGGGAG CGCCGGACCG GAGCGGAGCC CCGGGCGGCT CGCTGCTGCC CCCTAGCGGG
     5101 GGAGGGACGT AATTACATCC CTGGGGGCTT TGGGGGGGGG CTGTCCCTGA TATCTATAAC
     5161 AAGAAAATAT ATATATAATA AGTTATCACG TAAGTAGAAC ATGAAATAAC AATATAATTA
     5221 TCGTATGAGT TAAATCTTAA AAGTCACGTA AAAGATAATC ATGCGTCATT TTGACTCACG
     5281 CGGTCGTTAT AGTTCAAAAT CAGTGACACT TACCGCATTG ACAAGCACGC CTCACGGGAG
     5341 CTCCAAGCGG CGACTGAGAT GTCCTAAATG CACAGCGACG GATTCGCGCT ATTTAGAAAG
     5401 AGAGAGCAAT ATTTCAAGAA TGCATGCGTC AATTTTACGC AGACTATCTT TCTAGGGTTA
     5461 ATCTAGCTGC ATCAGGATCA TATCGTCGGG TCTTTTTTCC GGCTCAGTCA TCGCCCAAGC
     5521 TGGCGCTATC TGGGCATCGG GGAGGAAGAA GCCCGTGCCT TTTCCCGCGA GGTTGAAGCG
     5581 GCATGGAAAG AGTTTGCCGA GGATGACTGC TGCTGCATTG ACGTTGAGCG AAAACGCACG
     5641 TTTACCATGA TGATTCGGGA AGGTGTGGCC ATGCACGCCT TTAACGGTGA ACTGTTCGTT
     5701 CAGGCCACCT GGGATACCAG TTCGTCGCGG CTTTTCCGGA CACAGTTCCG GATGGTCAGC
     5761 CCGAAGCGCA TCAGCAACCC GAACAATACC GGCGACAGCC GGAACTGCCG TGCCGGTGTG
     5821 CAGATTAATG ACAGCGGTGC GGCGCTGGGA TATTACGTCA GCGAGGACGG GTATCCTGGC
     5881 TGGATGCCGC AGAAATGGAC ATGGATACCC CGTGAGTTAC CCGGCGGGCG CGCTTGGCGT
     5941 AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA
     6001 TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT
     6061 TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT
     6121 AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT
     6181 CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA
     6241 AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA
     6301 AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC
     6361 TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA
     6421 CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC
     6481 CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT
     6541 CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT
     6601 GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG
     6661 AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA
     6721 GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT
     6781 ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA
     6841 GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT
     6901 GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA
     6961 CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT
     7021 CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA
     7081 GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT
     7141 CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA
     7201 CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT
     7261 CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG
     7321 GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA
     7381 GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT
     7441 CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA
     7501 CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA
     7561 GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA
     7621 CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT
     7681 GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG
     7741 CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC
     7801 TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT
     7861 GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA
     7921 ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCAT
//