LOCUS       pUC-ccdB(2.3k)-T2c_BCD_ 3278 bp ds-DNA     circular     27-JUN-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     Seq Primer        1..24
                       /label="SD_seq.part.fwd"
                       /ApEinfo_revcolor="#c7b0e3"
                       /ApEinfo_fwdcolor="#c7b0e3"
     Spacer            65..94
                       /label="IDT Spacer 1"
                       /ApEinfo_revcolor="#ffef86"
                       /ApEinfo_fwdcolor="#ffef86"
     ribozyme          110..184
                       /label="riboJ"
                       /ApEinfo_revcolor="#ffef86"
                       /ApEinfo_fwdcolor="#ffef86"
     misc_feature      202..210
                       /label="RBS1"
                       /ApEinfo_revcolor="#84b0dc"
                       /ApEinfo_fwdcolor="#84b0dc"
     misc_feature      210..268
                       /label="T2C BCD 0.2k"
                       /ApEinfo_revcolor="#b7e6d7"
                       /ApEinfo_fwdcolor="#b7e6d7"
     CDS               217..267
                       /label="Translation 217-267"
                       /translation="MKAIFVLKHLNHAHDP*"
     misc_feature      255..263
                       /label="rbs2"
                       /ApEinfo_revcolor="#9eafd2"
                       /ApEinfo_fwdcolor="#9eafd2"
     misc_feature      269..272
                       /label="overhang"
                       /ApEinfo_revcolor="#c6c9d1"
                       /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature      270..272
                       /label="Start Codon"
                       /ApEinfo_revcolor="#85dae9"
                       /ApEinfo_fwdcolor="#85dae9"
     Spacer            284..313
                       /label="IDT Spacer 2"
                       /ApEinfo_revcolor="#ffef86"
                       /ApEinfo_fwdcolor="#ffef86"
     Seq Primer        complement(352..376)
                       /label="SD_seq.part.rev"
                       /ApEinfo_revcolor="#c7b0e3"
                       /ApEinfo_fwdcolor="#c7b0e3"
     Terminator        361..421
                       /label="L3S2P21 Terminator"
                       /ApEinfo_revcolor="#c6c9d1"
                       /ApEinfo_fwdcolor="#c6c9d1"
     Seq Primer        417..434
                       /label="seqPartMarkerfwd"
                       /ApEinfo_revcolor="#c7b0e3"
                       /ApEinfo_fwdcolor="#c7b0e3"
     Promoter          526..554
                       /label="bla Promoter"
                       /ApEinfo_revcolor="#85dae9"
                       /ApEinfo_fwdcolor="#85dae9"
     Resistance Marker 596..1456
                       /label="bla TEM-1"
                       /ApEinfo_revcolor="#9eafd2"
                       /ApEinfo_fwdcolor="#9eafd2"
     Terminator        1495..1543
                       /label="bla Terminator"
                       /ApEinfo_revcolor="#c6c9d1"
                       /ApEinfo_fwdcolor="#c6c9d1"
     Join              1571..1596
                       /label="Overlap from Part Vector"
                       /ApEinfo_revcolor="#d6b295"
                       /ApEinfo_fwdcolor="#d6b295"
     misc_feature      1625..1630
                       /label=-35
                       /ApEinfo_revcolor="#84b0dc"
                       /ApEinfo_fwdcolor="#84b0dc"
     misc_feature      1654..1673
                       /label="T2B spacer"
                       /ApEinfo_revcolor="#c6c9d1"
                       /ApEinfo_fwdcolor="#c6c9d1"
     ribozyme          1678..1752
                       /label="riboJ"
                       /ApEinfo_revcolor="#ffef86"
                       /ApEinfo_fwdcolor="#ffef86"
     misc_feature      1778..1836
                       /label="T2C BCD 2.3k"
                       /ApEinfo_revcolor="#b7e6d7"
                       /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature      1823..1831
                       /label="RBS2"
                       /ApEinfo_revcolor="#84b0dc"
                       /ApEinfo_fwdcolor="#84b0dc"
     CDS               1841..2146
                       /label="ccdB"
                       /ApEinfo_revcolor="#c7b0e3"
                       /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature      2278..2310
                       /label="term748"
                       /ApEinfo_revcolor="#b1ff67"
                       /ApEinfo_fwdcolor="#b1ff67"
     misc_feature      complement(2406..2994)
                       /label="ori"
                       /ApEinfo_revcolor="#c6c9d1"
                       /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature      complement(3041..3072)
                       /label="seqAL5000Sinsrev"
                       /ApEinfo_revcolor="#ff0080"
                       /ApEinfo_fwdcolor="#ff0080"
     Terminator        3235..3278
                       /label="Synthetic Bidirectional Terminator"
                       /ApEinfo_revcolor="#c6c9d1"
                       /ApEinfo_fwdcolor="#c6c9d1"
ORIGIN
        1 acagggctat aaggtagatc cgggtcgacA GATATAATTA GCCAAAAATT TCTGTAATAA
       61 ATATCACTAG CTCAGATTCA GTAGACCGCT GTTGGATAgg tctctGGAGa gctgtcaccg
      121 gatgtgcttt ccggtctgat gagtccgtga ggacgaaaca gcctctaCAA ATAATTTTGT
      181 TTAAGGGCCC AAGTTCACTT AAAAAGGAGA TCAACAATGA AAGCAATTTT CGTACTGAAA
      241 CATCTTAATC ATGCTCATGA CCCTTAAAta tgagagaccA TCGTGCATGA TCTACGTGCG
      301 TCACATGCAG TACctgaatt gactctcttc cgggGAtcaa atgtagcacc tgaagtcaCc
      361 CTCGGTACCA AATTCCAGAA AAGAGGCCTC CCGAAAGGGG GGCCTTTTTT CGTTTTGGTC
      421 Cgttactgga acgttgtgag gTACAcggaa gctgtggtat ggctgtgcag gtcgtaaatc
      481 actgcataat tcgtgtcgct caaggcgcat atttttctaa atacattcaa atatgtatcc
      541 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
      601 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
      661 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
      721 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
      781 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt
      841 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
      901 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
      961 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     1021 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     1081 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc
     1141 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     1201 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     1261 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg gAtcGcgcgg
     1321 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     1381 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     1441 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     1501 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     1561 aatcccttaa cgtgagtttt cgttccactg agcgtcGAGT ggctgtcgac CggcGAAAAG
     1621 TTCGAtTaca gctagctcag tcctaggtaC AATtacagCC Atcgtacgag cccGGAGagc
     1681 tgtcaccgga tgtgctttcc ggtctgatga gtccgtgagg acgaaacagc ctctaCAAAT
     1741 AATTTTGTTT AAGGGCCCAA GTTCACTTAA AAAGGAGATC AACAATGAAA GCAATTTTCG
     1801 TACTGAAACA TCTTAATCAT GCTATGTGTT TTTAAAtatg atgcagttta aggtttacac
     1861 ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac
     1921 gcccgggcga cggatggtga tccccctggc cagtgcacgt ctgctgtcag ataaagtctc
     1981 ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga
     2041 tatggccagt gtgccggtgt ccgttatcgg ggaagaagtg gctgatctca gccaccgcga
     2101 aaatgacatc aaaaacgcca ttaacctgat gttctgggga atataaATCC gttttagttc
     2161 gtcggtcagt ttcacctgat ttacgtaaaa acccgcttcg gcgggttttt gcttttggag
     2221 gggcagaaag atgaatgact gtcGCTGtgt acgcgataga taaaaaaaaa ggtttagaca
     2281 gtgcgaaagc actgtctaaa cctttttttt CCTGCTCAGG CGCAACCGAt catgaccaaa
     2341 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     2401 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     2461 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     2521 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     2581 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     2641 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2701 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2761 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     2821 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2881 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2941 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     3001 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     3061 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     3121 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcCCCTgttt cccgactgga
     3181 aagcggcgac Tcctgcatta ggaaattata aacgcaggtc ttttcgccgg tgtatttctt
     3241 gcaaaaaaaa accccgccga agcggggttt tttttttt
//