LOCUS       untagged_WWP1          10135 bp    DNA     circular SYN 21-MAR-2023
DEFINITION  Ligation of WWP1 insert into pQCXIN-NotI, EcoRI
ACCESSION   untagged_WWP1
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 10135)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  pQCXIN-NotI, EcoRI
               Fragment ends: EcoRI and NotI
               Fragment size: 7358
            Insert molecule:  WWP1 insert
               Fragment ends: NotI and EcoRI
               Fragment size: 2777
FEATURES             Location/Qualifiers
     CDS             2251..5019
                     /gene="WWP1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             5630..6424
                     /gene="NeoR"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="NeoR"
ORIGIN      
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
     1561 aggcgccgga attgaagatc tgggggatcg atcctctaga gtccgttaca taacttacgg
     1621 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     1681 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     1741 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     1801 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     1861 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     1921 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     1981 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     2041 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     2101 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     2161 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa
     2221 cggacctgca ggaattgatc cgcggccgca atggccactg cttcaccaag gtctgatact
     2281 agtaataacc acagtggaag gttgcagtta caggtaactg tttctagtgc caaacttaaa
     2341 agaaaaaaga actggttcgg aacagcaata tatacagaag tagttgtaga tggagaaatt
     2401 acgaaaacag caaaatccag tagttcttct aatccaaaat gggatgaaca gctaactgta
     2461 aatgttacgc cacagactac attggaattt caagtttgga gccatcgcac tttaaaagca
     2521 gatgctttat taggaaaagc aacgatagat ttgaaacaag ctctgttgat acacaataga
     2581 aaattggaaa gagtgaaaga acaattaaaa ctttccttgg aaaacaagaa tggcatagca
     2641 caaactggtg aattgacagt tgtgcttgat ggattggtga ttgagcaaga aaatataaca
     2701 aactgcagct catctccaac catagaaata caggaaaatg gtgatgcctt acatgaaaat
     2761 ggagagcctt cagcaaggac aactgccagg ttggctgttg aaggcacgaa tggaatagat
     2821 aatcatgtac ctacaagcac tctagtccaa aactcatgct gctcgtatgt agttaatgga
     2881 gacaacacac cttcatctcc gtctcaggtt gctgccagac ccaaaaatac accagctcca
     2941 aaaccactcg catctgagcc tgccgatgac actgttaatg gagaatcatc ctcatttgca
     3001 ccaactgata atgcgtctgt cacgggtact ccagtagtgt ctgaagaaaa tgccttgtct
     3061 ccaaattgca ctagtactac tgttgaagat cctccagttc aagaaatact gacttcctca
     3121 gaaaacaatg aatgtattcc ttctaccagt gcagaattgg aatctgaagc tagaagtata
     3181 ttagagcctg acacctctaa ttctagaagt agttctgctt ttgaagcagc caaatcaaga
     3241 cagccagatg ggtgtatgga tcctgtacgg cagcagtctg ggaatgccaa cacagaaacc
     3301 ttgccatcag ggtgggaaca aagaaaagat cctcatggta gaacctatta tgtggatcat
     3361 aatactcgaa ctaccacatg ggagagacca caacctttac ctccaggttg ggaaagaaga
     3421 gttgatgatc gtagaagagt ttattatgtg gatcataaca ccagaacaac aacgtggcag
     3481 cggcctacca tggaatctgt ccgaaatttt gaacagtggc aatctcagcg gaaccaattg
     3541 cagggagcta tgcaacagtt taaccaacga tacctctatt cggcttcaat gttagctgca
     3601 gaaaatgacc cttatggacc tttgccacca ggctgggaaa aaagagtgga ttcaacagac
     3661 agggtttact ttgtgaatca taacacaaaa acaacccagt gggaagatcc aagaactcaa
     3721 ggcttacaga atgaagaacc cctgccagaa ggctgggaaa ttagatatac tcgtgaaggt
     3781 gtaaggtact ttgttgatca taacacaaga acaacaacat tcaaagatcc tcgcaatggg
     3841 aagtcatctg taactaaagg tggtccacaa attgcttatg aacgcggctt taggtggaag
     3901 cttgctcact tccgttattt gtgccagtct aatgcactac ctagtcatgt aaagatcaat
     3961 gtgtcccggc agacattgtt tgaagattcc ttccaacaga ttatggcatt aaaaccctat
     4021 gacttgagga ggcgcttata tgtaatattt agaggagaag aaggacttga ttatggtggc
     4081 ctagcgagag aatggttttt cttgctttca catgaagttt tgaacccaat gtattgctta
     4141 tttgagtatg cgggcaagaa caactattgt ctgcagataa atccagcatc aaccattaat
     4201 ccagaccatc tttcatactt ctgtttcatt ggtcgtttta ttgccatggc actatttcat
     4261 ggaaagttta tcgatactgg tttctcttta ccattctaca agcgtatgtt aagtaaaaaa
     4321 cttactatta aggatttgga atctattgat actgaatttt ataactccct tatctggata
     4381 agagataaca acattgaaga atgtggctta gaaatgtact tttctgttga catggagatt
     4441 ttgggaaaag ttacttcaca tgacctgaag ttgggaggtt ccaatattct ggtgactgag
     4501 gagaacaaag atgaatatat tggtttaatg acagaatggc gtttttctcg aggagtacaa
     4561 gaacagacca aagctttcct tgatggtttt aatgaagttg ttcctcttca gtggctacag
     4621 tacttcgatg aaaaagaatt agaggttatg ttgtgtggca tgcaggaggt tgacttggca
     4681 gattggcaga gaaatactgt ttatcgacat tatacaagaa acagcaagca aatcatttgg
     4741 ttttggcagt ttgtgaaaga gacagacaat gaagtaagaa tgcgactatt gcagttcgtc
     4801 actggaacct gccgtttacc tctaggagga tttgctgagc tcatgggaag taatgggcct
     4861 caaaagtttt gcattgaaaa agttggcaaa gacacttggt taccaagaag ccatacatgt
     4921 tttaatcgct tggatctacc accatataag agttatgaac aactaaagga aaaacttctt
     4981 tttgcaatag aagagacaga gggatttgga caagaatgag aattccgccc cccccccccc
     5041 cccccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg
     5101 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc
     5161 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
     5221 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga
     5281 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc
     5341 ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc
     5401 cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac
     5461 aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg
     5521 tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac
     5581 ggggacgtgg ttttcctttg aaaaacacga tgataagctt gccacaacca tggctgaaca
     5641 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     5701 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     5761 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc
     5821 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     5881 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     5941 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     6001 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     6061 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     6121 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct
     6181 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     6241 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     6301 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     6361 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     6421 ctgagcggga tcggctagag tcgacccgga cgggacgctc ctgcgcctga tacagaacga
     6481 attgcttgca ggcatctcat gagtgtgtct tcccgttttc cgcctgaggt cactgcgtgg
     6541 atggagcgct ggcgcctgct gcgcgacggc gagctgctca ccacccactc gagggctgca
     6601 gcgctgcaga ggccgagtgc agaactgctc caaagggacc tcaaggcttt ccgagggaca
     6661 ctaggctgac tccatcgagc cagtgtagag ataagcttat cgattagtcc aatttgttaa
     6721 agacaggata tcagtggtcc aggctctagt tttgactcaa caatatcacc agctgaagcc
     6781 tatagagtac gagccataga taaaataaaa gattttattt agtctccaga aaaagggggg
     6841 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
     6901 atggaaaaat acataactga gaatagagaa gttcagatca aggtcaggaa cagatggaac
     6961 agggtcgacc ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc
     7021 ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc
     7081 tccccgagct caataaaaga gcccacaacc cctcactcgg ggcgccagtc ctccgattga
     7141 ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt gcagttgcat ccgacttgtg
     7201 gtctcgctgt tccttgggag ggtctcctct gagtgattga ctacccgtca gcgggggtct
     7261 ttcatttggg ggctcgtccg ggatcgggag acccctgccc agggaccacc gacccaccac
     7321 cgggaggtaa gctggctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     7381 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     7441 tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag
     7501 cgatagcgga gtgtagatcc ggctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     7561 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     7621 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     7681 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     7741 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     7801 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     7861 aaagcttact ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
     7921 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct
     7981 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     8041 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     8101 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     8161 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     8221 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     8281 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     8341 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     8401 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     8461 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     8521 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     8581 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     8641 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     8701 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     8761 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     8821 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     8881 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     8941 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     9001 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     9061 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     9121 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     9181 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg
     9241 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     9301 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     9361 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     9421 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     9481 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat
     9541 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     9601 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     9661 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     9721 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     9781 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     9841 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     9901 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     9961 aggccctttc gtcttcaaga attagcttgg ccattgcata cgttgtatcc atatcataat
    10021 atgtacattt atattggctc atgtccaaca ttaccgccat gttgacattg attattgact
    10081 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagt
//
