LOCUS       Exported                9982 bp ds-DNA     circular SYN 16-MAR-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9982)
  AUTHORS   Morita lab
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Mar 20, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9982
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        309..512
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             513..688
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    751..950
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1151..1567
                     /label=gag (truncated)
                     /note="truncated MMLV gag gene lacking the start codon"
     enhancer        1604..1907
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1908..2111
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      2279..2288
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             2288..2320
                     /codon_start=1
                     /label=nluc9
                     /translation="GSLLFRVTING"
     CDS             2381..2486
                     /codon_start=1
                     /label=E5
                     /translation="EVSALEKEVSALEKEVSALEKEVSALEKEVSALEK"
     CDS             2492..2524
                     /codon_start=1
                     /label=HiBiT
                     /translation="VSGWRLFKKIS"
     CDS             2534..2557
                     /codon_start=1
                     /label=Caspase3 cleavage site
                     /translation="GDEVDGGS"
     CDS             2558..2663
                     /codon_start=1
                     /label=K5
                     /translation="KVSALKEKVSALKEKVSALKEKVSALKEKVSALKE"
     CDS             2669..2722
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2735..3180
                     /codon_start=1
                     /label=LgBiT
                     /translation="MVFTLEDFVGDWEQTAAYNLDQVLEQGGVSSLLQNLAVSVTPIQR
                     IVRSGENALKIDIHVIIPYEGLSADQMAQIEEVFKVVYPVDDHHFKVILPYGTLVIDGV
                     TPNMLNYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLITPD"
     CDS             3182..3205
                     /codon_start=1
                     /label=Caspase3 cleavage site
                     /translation="GDEVDGGS"
     CDS             3227..3274
                     /codon_start=1
                     /product="non-ORF yeast peptide conferring 
                     ubiquitin-dependent degradation"
                     /label=hCL1
                     /note="human codon-optimized"
                     /translation="ACKNWFSSLSHFVIHL"
     CDS             3278..3397
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     CDS             3413..3469
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3476..5125
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     misc_feature    5132..5704
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             5730..6329
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     LTR             6688..7113
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     promoter        7379..7708
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7559..7694
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(8001..8589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8760..9620)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9621..9725)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        join(9911..9982,1..308)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
     1561 aggcgccgga attgaagatc tgggggatcg atcctctaga gtccgttaca taacttacgg
     1621 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     1681 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     1741 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     1801 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     1861 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     1921 ggcagtacat caatgggcgt gaatagcggt ttgactcacg gggatttcca agtctccacc
     1981 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     2041 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     2101 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     2161 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa
     2221 cggacctgca ggaattgatc cgcggccgca ccggtaggcc tcgtacgctt aattaacggc
     2281 caccatgggc tccctgctgt tccgagtaac catcaacggc ggcggtggag gctccggggg
     2341 aggcgggtct ggcggagggg gaagtggcgg tggtggctct gaagttagcg ctctggaaaa
     2401 agaagtgtct gcactcgaga aagaagtaag tgcccttgag aaggaggtgt ccgcactcga
     2461 gaaggaggtc agcgccctgg aaaaggaaaa ggtgagtggg tggcgacttt tcaaaaaaat
     2521 ctccggatcc tcaggagatg aggtggacgg tggaagcaag gtgtccgccc tgaaggaaaa
     2581 agtaagcgca ctgaaagaaa aggtgagcgc gctgaaggag aaagtgagcg ccctgaaaga
     2641 gaaagtctct gcccttaagg aggaattcga gggcagagga agtctgctaa catgcggtga
     2701 cgtcgaggag aatcctggcc caaagcttgc caccatggtt ttcacattgg aagacttcgt
     2761 gggtgactgg gagcagactg ccgcgtacaa cttggatcaa gttctggagc aaggtggagt
     2821 aagctccctc ctgcaaaacc ttgcggtaag cgttactccc atccaaagga tcgtaaggtc
     2881 cggggaaaat gctctcaaaa tagatataca cgttatcata ccttacgaag ggctgtctgc
     2941 cgatcagatg gctcaaatag aagaagtttt caaagtagtc taccccgtgg acgatcatca
     3001 ttttaaggtg attctccctt atgggaccct cgttatcgac ggggttacac ctaatatgct
     3061 caattacttc ggaaggccat atgaaggaat agctgttttc gatggtaaaa agattacggt
     3121 caccggcact ctctggaatg ggaataagat aatagatgag agactgataa cgccagacgg
     3181 cggagatgag gtggacggtg gaagccaatt gggaggcagc gaagtggctt gcaagaactg
     3241 gttcagtagc ttaagccact ttgtgatcca ccttaattct cacggctttc cgcctgaggt
     3301 tgaagagcaa gccgccggta cattgcctat gtcctgcgca caagaaagcg gtatggaccg
     3361 gcacccagcc gcttgtgctt cagctcgcat caacgtcggc ggccgctcta gagcaaccaa
     3421 tttcagcctt cttaaacagg ctggagatgt ggaagagaat cctggaccag aattgatgga
     3481 agatgccaaa aacattaaga agggcccagc gccattctac ccactcgaag acgggaccgc
     3541 cggcgagcag ctgcacaaag ccatgaagcg ctacgccctg gtgcccggca ccatcgcctt
     3601 taccgacgca catatcgagg tggacattac ctacgccgag tacttcgaga tgagcgttcg
     3661 gctggcagaa gctatgaagc gctatgggct gaatacaaac catcggatcg tggtgtgcag
     3721 cgagaatagc ttgcagttct tcatgcccgt gttgggtgcc ctgttcatcg gtgtggctgt
     3781 ggccccagct aacgacatct acaacgagcg cgagctgctg aacagcatgg gcatcagcca
     3841 gcccaccgtc gtattcgtga gcaagaaagg gctgcaaaag atcctcaacg tgcaaaagaa
     3901 gctaccgatc atacaaaaga tcatcatcat ggatagcaag accgactacc agggcttcca
     3961 aagcatgtac accttcgtga cttcccattt gccacccggc ttcaacgagt acgacttcgt
     4021 gcccgagagc ttcgaccggg acaaaaccat cgccctgatc atgaacagta gtggcagtac
     4081 cggattgccc aagggcgtag ccctaccgca ccgcaccgct tgtgtccgat tcagtcatgc
     4141 ccgcgacccc atcttcggca accagatcat ccccgacacc gctatcctca gcgtggtgcc
     4201 atttcaccac ggcttcggca tgttcaccac gctgggctac ttgatctgcg gctttcgggt
     4261 cgtgctcatg taccgcttcg aggaggagct attcttgcgc agcttgcaag actataagat
     4321 tcaatctgcc ctgctggtgc ccacactatt tagcttcttc gctaagagca ctctcatcga
     4381 caagtacgac ctaagcaact tgcacgagat cgccagcggc ggggcgccgc tcagcaagga
     4441 ggtaggtgag gccgtggcca aacgcttcca cctaccaggc atccgccagg gctacggcct
     4501 gacagaaaca accagcgcca ttctgatcac ccccgaaggg gacgacaagc ctggcgcagt
     4561 aggcaaggtg gtgcccttct tcgaggctaa ggtggtggac ttggacaccg gtaagacact
     4621 gggtgtgaac cagcgcggcg agctgtgcgt ccgtggcccc atgatcatga gcggctacgt
     4681 taacaacccc gaggctacaa acgctctcat cgacaaggac ggctggctgc acagcggcga
     4741 catcgcctac tgggacgagg acgagcactt cttcatcgtg gaccggctga agagcctgat
     4801 caaatacaag ggctaccagg tagccccagc cgaactggag agcatcctgc tgcaacaccc
     4861 caacatcttc gacgccgggg tcgccggcct gcccgacgac gatgccggcg agctgcccgc
     4921 cgcagtcgtc gtgctggaac acggtaaaac catgaccgag aaggagatcg tggactatgt
     4981 ggccagccag gttacaaccg ccaagaagct gcgcggtggt gttgtgttcg tggacgaggt
     5041 gcctaaagga ctgaccggca agttggacgc ccgcaagatc cgcgagattc tcattaaggc
     5101 caagaagggc ggcaagatcg ccgtgtaacc gcccctctcc ctcccccccc cctaacgtta
     5161 ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca
     5221 tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca
     5281 ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg
     5341 aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc
     5401 agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata
     5461 cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag
     5521 tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc
     5581 attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt
     5641 taaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg
     5701 ataagcttgc cacaaggaga cgaccttcca tgaccgagta caagcccacg gtgcgcctcg
     5761 ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc
     5821 ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag
     5881 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
     5941 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
     6001 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
     6061 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggagtct
     6121 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
     6181 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
     6241 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
     6301 ggtgcatgac ccgcaagccc ggtgcctgat gcttgcaggc atctcatgag tgtgtcttcc
     6361 cgttttccgc ctgaggtcac tgcgtggatg gagcgctggc gcctgctgcg cgacggcgag
     6421 ctgctcacca cccactcgag ggctgcagcg ctgcagaggc cgagtgcaga actgctccaa
     6481 agggacctca aggctttccg agggacacta ggctgactcc atcgagccag tgtagagata
     6541 agcttatcga ttagtccaat ttgttaaaga caggatatca gtggtccagg ctctagtttt
     6601 gactcaacaa tatcaccagc tgaagcctat agagtacgag ccatagataa aataaaagat
     6661 tttatttagt ctccagaaaa aggggggaat gaaagacccc acctgtaggt ttggcaagct
     6721 agcttaagta acgccatttt gcaaggcatg gaaaaataca taactgagaa tagagaagtt
     6781 cagatcaagg tcaggaacag atggaacagg gtcgacccta gagaaccatc agatgtttcc
     6841 agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca atcagttcgc
     6901 ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc cacaacccct
     6961 cactcggggc gccagtcctc cgattgactg agtcgcccgg gtacccgtgt atccaataaa
     7021 ccctcttgca gttgcatccg acttgtggtc tcgctgttcc ttgggagggt ctcctctgag
     7081 tgattgacta cccgtcagcg ggggtctttc atttgggggc tcgtccggga tcgggagacc
     7141 cctgcccagg gaccaccgac ccaccaccgg gaggtaagct ggctgcctcg cgcgtttcgg
     7201 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
     7261 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
     7321 gggcgcagcc atgacccagt cacgtagcga tagcggagtg tagatccggc tgtggaatgt
     7381 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     7441 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     7501 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     7561 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     7621 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     7681 cttttttgga ggcctaggct tttgcaaaaa gcttactggc ttaactatgc ggcatcagag
     7741 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     7801 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     7861 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     7921 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     7981 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     8041 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     8101 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     8161 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     8221 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     8281 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     8341 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     8401 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     8461 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     8521 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     8581 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     8641 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     8701 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     8761 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     8821 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     8881 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     8941 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     9001 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     9061 gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     9121 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     9181 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     9241 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     9301 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     9361 tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     9421 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     9481 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     9541 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     9601 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     9661 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     9721 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     9781 ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaagaatt agcttggcca
     9841 ttgcatacgt tgtatccata tcataatatg tacatttata ttggctcatg tccaacatta
     9901 ccgccatgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
     9961 gttcatagcc catatatgga gt
//
