LOCUS       Exported                1791 bp ds-DNA     circular SYN 20-MAR-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Mar 20, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Ligation of : pYTK001.gb.xdna [1642  nt] : (#BsmBI[1024] / 
            #BsmBI[6]) to PCR(Sequence Window #2) [141  nt] : (#BsmBI[5] / 
            #BsmBI[162])
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     5..145
                     /note="note=""Fragment extracted from PCR(Sequence Window 
                     #2) [141  nt] : (#BsmBI[5] / #BsmBI[162])"
     misc_feature    14..136
                     /label=Kar2-SS
                     /note="note=""direct strand 
                     translation""translation=""MFFNRLSAGKLLVPLSVVLYALFVVILPLQNS
                     FHSSNVLVRG"""
     misc_feature    complement(152..260)
                     /label=CamR Terminator
                     /note="label=CamR Terminator - ApEinfo_fwdcolor=#66ccff - 
                     ApEinfo_revcolor=#66ccff - ApEinfo_graphicformat=arrow_data
                     {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 
                     complement(1037..1145)"
     CDS             complement(261..920)
                     /label=CamR
                     /note="label=CamR - ApEinfo_fwdcolor=#0000FF - 
                     ApEinfo_revcolor=#0000FF - ApEinfo_graphicformat=arrow_data
                     {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 
                     complement(1146..1805)"
     promoter        complement(921..1025)
                     /label=CamR Promoter
                     /note="label=CamR Promoter - ApEinfo_fwdcolor=#66CCFF - 
                     ApEinfo_revcolor=#66CCFF - ApEinfo_graphicformat=arrow_data
                     {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 
                     complement(1806..1910)"
     rep_origin      complement(1026..1789)
                     /direction=LEFT
                     /label=ColE1
                     /note="label=ColE1 - ApEinfo_fwdcolor=#7F7F7F - 
                     ApEinfo_revcolor=#7F7F7F - ApEinfo_graphicformat=arrow_data
                     {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 
                     complement(1911..2674)"
ORIGIN
        1 tcggtctcat atgtttttca acagactaag cgctggcaag ctgctggtac cactctccgt
       61 ggtcctgtac gcccttttcg tggtaatatt acctttacag aattctttcc actcctccaa
      121 tgttttagtt agaggtggtt cttgagacca gaccaataaa aaacgcccgg cggcaaccga
      181 gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc
      241 caagcgagct cgatatcaaa ttacgccccg ccctgccact catcgcagta ctgttgtaat
      301 tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc
      361 agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg
      421 aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg
      481 gctgaaacga aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg
      541 taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca
      601 ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca
      661 ctatcccata tcaccagctc accgtctttc attgccatac gaaattccgg atgagcattc
      721 atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg
      781 gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact
      841 gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat
      901 ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa
      961 aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgcccg
     1021 atcaatcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     1081 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1141 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1201 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     1261 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     1321 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     1381 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     1441 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     1501 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     1561 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     1621 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     1681 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     1741 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta g
//
