LOCUS       Exported                1725 bp ds-DNA     circular SYN 20-MAR-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Mar 20, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Ligation of : pYTK001.gb.xdna [1642  nt] : (#BsmBI[1024] / 
            #BsmBI[6]) to Sequence Window #2 [75  nt] : (#BsmBI[1] / #BsmBI[92])
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     5..71
                     /note="note=""Fragment extracted from Sequence Window #2 
                     [75  nt] : (#BsmBI[1] / #BsmBI[92])"
     misc_feature    14..70
                     /label=BPSV40
                     /note="note=""direct strand 
                     translation""translation=""KRTADGSEFESPKKKRKVE"""
                     74..76
                     /note="note=""Fragment extracted from Sequence Window #2 
                     [75  nt] : (#BsmBI[1] / #BsmBI[92])"
                     77..79
                     /note="note=""Fragment extracted from Sequence Window #2 
                     [75  nt] : (#BsmBI[1] / #BsmBI[92])"
     misc_feature    complement(86..194)
                     /label=CamR Terminator
                     /note="label=CamR Terminator - ApEinfo_fwdcolor=#66ccff - 
                     ApEinfo_revcolor=#66ccff - ApEinfo_graphicformat=arrow_data
                     {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 
                     complement(1037..1145)"
     CDS             complement(195..854)
                     /label=CamR
                     /note="label=CamR - ApEinfo_fwdcolor=#0000FF - 
                     ApEinfo_revcolor=#0000FF - ApEinfo_graphicformat=arrow_data
                     {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 
                     complement(1146..1805)"
     promoter        complement(855..959)
                     /label=CamR Promoter
                     /note="label=CamR Promoter - ApEinfo_fwdcolor=#66CCFF - 
                     ApEinfo_revcolor=#66CCFF - ApEinfo_graphicformat=arrow_data
                     {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 
                     complement(1806..1910)"
     rep_origin      complement(960..1723)
                     /direction=LEFT
                     /label=ColE1
                     /note="label=ColE1 - ApEinfo_fwdcolor=#7F7F7F - 
                     ApEinfo_revcolor=#7F7F7F - ApEinfo_graphicformat=arrow_data
                     {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 
                     complement(1911..2674)"
ORIGIN
        1 tcggtctcat atgaaaagaa ctgctgatgg ttctgaattt gaatctccaa aaaaaaaaag
       61 aaaagttgaa ggttcttgag accagaccaa taaaaaacgc ccggcggcaa ccgagcgttc
      121 tgaacaaatc cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg
      181 agctcgatat caaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta
      241 agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc
      301 atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag
      361 ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgaa
      421 acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac
      481 gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag
      541 agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc
      601 catatcacca gctcaccgtc tttcattgcc atacgaaatt ccggatgagc attcatcagg
      661 cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt
      721 aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga
      781 aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg
      841 atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg
      901 cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg cccgatcaat
      961 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1021 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1081 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1141 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     1201 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1261 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1321 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     1381 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     1441 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     1501 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     1561 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     1621 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     1681 catgttcttt cctgcgttat cccctgattc tgtggataac cgtag
//
