LOCUS       Exported                1815 bp ds-DNA     circular SYN 20-MAR-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Mar 20, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Ligation of : pYTK001.gb.xdna [1642  nt] : (#BsmBI[1024] / 
            #BsmBI[6]) to PCR(Sequence Window #4) [165  nt] : (#BsmBI[5] / 
            #BsmBI[186])
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     5..169
                     /note="note=""Fragment extracted from PCR(Sequence Window 
                     #4) [165  nt] : (#BsmBI[5] / #BsmBI[186])"
     misc_feature    14..160
                     /label=PTS2 POT1 (2-50)
                     /note="note=""direct strand 
                     translation""translation=""SQRLQSIKDHLVESAMGKGESKRKNSLLEKRP
                     EDVVIVAANRSAIGKGF"""
     misc_feature    complement(176..284)
                     /label=CamR Terminator
                     /note="label=CamR Terminator - ApEinfo_fwdcolor=#66ccff - 
                     ApEinfo_revcolor=#66ccff - ApEinfo_graphicformat=arrow_data
                     {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 
                     complement(1037..1145)"
     CDS             complement(285..944)
                     /label=CamR
                     /note="label=CamR - ApEinfo_fwdcolor=#0000FF - 
                     ApEinfo_revcolor=#0000FF - ApEinfo_graphicformat=arrow_data
                     {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 
                     complement(1146..1805)"
     promoter        complement(945..1049)
                     /label=CamR Promoter
                     /note="label=CamR Promoter - ApEinfo_fwdcolor=#66CCFF - 
                     ApEinfo_revcolor=#66CCFF - ApEinfo_graphicformat=arrow_data
                     {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 
                     complement(1806..1910)"
     rep_origin      complement(1050..1813)
                     /direction=LEFT
                     /label=ColE1
                     /note="label=ColE1 - ApEinfo_fwdcolor=#7F7F7F - 
                     ApEinfo_revcolor=#7F7F7F - ApEinfo_graphicformat=arrow_data
                     {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 
                     complement(1911..2674)"
ORIGIN
        1 tcggtctcat atgtctcaaa gactacaaag tatcaaggat catttggtgg agagcgccat
       61 gggtaagggt gaatcgaaga ggaagaactc gttgctggag aaaagacccg aagatgtagt
      121 tattgtggct gctaacaggt ctgccatcgg taaaggtttt ggttcttgag accagaccaa
      181 taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca
      241 ttactggatc tatcaacagg agtccaagcg agctcgatat caaattacgc cccgccctgc
      301 cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa
      361 acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat
      421 ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa
      481 ctggtgaaac tcacccaggg attggctgaa acgaaaaaca tattctcaat aaacccttta
      541 gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac
      601 tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg
      661 aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc
      721 atacgaaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa
      781 aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc
      841 tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat
      901 tgggatatat caacggtggt atatccagtg atttttttct ccattttagc ttccttagct
      961 cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga
     1021 aagttggaac ctcttacgtg cccgatcaat catgaccaaa atcccttaac gtgagttttc
     1081 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     1141 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     1201 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     1261 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     1321 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     1381 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     1441 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     1501 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     1561 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     1621 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     1681 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     1741 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     1801 tgtggataac cgtag
//
