LOCUS       Exported                1759 bp ds-DNA     circular SYN 20-MAR-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Mar 20, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Ligation of : pYTK001.gb.xdna [1642  nt] : (#BsmBI[1024] / 
            #BsmBI[6]) to Sequence Window #5 [109  nt] : (#BsmBI[5] / 
            #BsmBI[130])
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     5..113
                     /note="note=""Fragment extracted from Sequence Window #5 
                     [109  nt] : (#BsmBI[5] / #BsmBI[130])"
     misc_feature    14..106
                     /label=PTS1 FOX2 (870-900)
                     /note="note=""direct strand 
                     translation""translation=""VVVFQTIDTTRNVIVLDNAAVKLSQAKSKL*"
                     ""
     misc_feature    complement(120..228)
                     /label=CamR Terminator
                     /note="label=CamR Terminator - ApEinfo_fwdcolor=#66ccff - 
                     ApEinfo_revcolor=#66ccff - ApEinfo_graphicformat=arrow_data
                     {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 
                     complement(1037..1145)"
     CDS             complement(229..888)
                     /label=CamR
                     /note="label=CamR - ApEinfo_fwdcolor=#0000FF - 
                     ApEinfo_revcolor=#0000FF - ApEinfo_graphicformat=arrow_data
                     {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 
                     complement(1146..1805)"
     promoter        complement(889..993)
                     /label=CamR Promoter
                     /note="label=CamR Promoter - ApEinfo_fwdcolor=#66CCFF - 
                     ApEinfo_revcolor=#66CCFF - ApEinfo_graphicformat=arrow_data
                     {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 
                     complement(1806..1910)"
     rep_origin      complement(994..1757)
                     /direction=LEFT
                     /label=ColE1
                     /note="label=ColE1 - ApEinfo_fwdcolor=#7F7F7F - 
                     ApEinfo_revcolor=#7F7F7F - ApEinfo_graphicformat=arrow_data
                     {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 
                     complement(1911..2674)"
ORIGIN
        1 tcggtctcaa tccgttgtcg tttttcaaac aattgatacg accagaaacg tcattgtatt
       61 ggataacgcc gctgtaaaac tatcgcaggc aaaatctaaa ctataatggc tgagaccaga
      121 ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag
      181 gtcattactg gatctatcaa caggagtcca agcgagctcg atatcaaatt acgccccgcc
      241 ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc
      301 acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata
      361 atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc
      421 aaaactggtg aaactcaccc agggattggc tgaaacgaaa aacatattct caataaaccc
      481 tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag
      541 aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc
      601 atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat
      661 tgccatacga aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg
      721 ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac
      781 ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg
      841 ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt
      901 agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg
      961 gtgaaagttg gaacctctta cgtgcccgat caatcatgac caaaatccct taacgtgagt
     1021 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     1081 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     1141 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     1201 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     1261 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     1321 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     1381 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     1441 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     1501 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     1561 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     1621 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     1681 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     1741 attctgtgga taaccgtag
//
