LOCUS       Exported                1716 bp ds-DNA     circular SYN 20-MAR-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Mar 20, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Ligation of : pYTK001.gb.xdna [1642  nt] : (#BsmBI[1024] / 
            #BsmBI[6]) to Sequence Window #2 [66  nt] : (#BsmBI[1] / #BsmBI[83])
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     5..62
                     /note="note=""Fragment extracted from Sequence Window #2 
                     [66  nt] : (#BsmBI[1] / #BsmBI[83])"
     misc_feature    14..61
                     /label=sfGFP11
                     /note="note=""direct strand 
                     translation""translation=""RDHMVLHEYVNAAGIT"""
                     65..67
                     /note="note=""Fragment extracted from Sequence Window #2 
                     [66  nt] : (#BsmBI[1] / #BsmBI[83])"
                     68..70
                     /note="note=""Fragment extracted from Sequence Window #2 
                     [66  nt] : (#BsmBI[1] / #BsmBI[83])"
     misc_feature    complement(77..185)
                     /label=CamR Terminator
                     /note="label=CamR Terminator - ApEinfo_fwdcolor=#66ccff - 
                     ApEinfo_revcolor=#66ccff - ApEinfo_graphicformat=arrow_data
                     {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 
                     complement(1037..1145)"
     CDS             complement(186..845)
                     /label=CamR
                     /note="label=CamR - ApEinfo_fwdcolor=#0000FF - 
                     ApEinfo_revcolor=#0000FF - ApEinfo_graphicformat=arrow_data
                     {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 
                     complement(1146..1805)"
     promoter        complement(846..950)
                     /label=CamR Promoter
                     /note="label=CamR Promoter - ApEinfo_fwdcolor=#66CCFF - 
                     ApEinfo_revcolor=#66CCFF - ApEinfo_graphicformat=arrow_data
                     {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 
                     complement(1806..1910)"
     rep_origin      complement(951..1714)
                     /direction=LEFT
                     /label=ColE1
                     /note="label=ColE1 - ApEinfo_fwdcolor=#7F7F7F - 
                     ApEinfo_revcolor=#7F7F7F - ApEinfo_graphicformat=arrow_data
                     {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 
                     complement(1911..2674)"
ORIGIN
        1 tcggtctcat atgagagatc atatggtttt gcatgaatat gttaatgctg ctggtattac
       61 tggttcttga gaccagacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat
      121 ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata
      181 tcaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg
      241 ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc
      301 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata
      361 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga aacgaaaaac
      421 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct
      481 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa
      541 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc
      601 agctcaccgt ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga
      661 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc
      721 gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca
      781 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc
      841 tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt
      901 gatcttattt cattatggtg aaagttggaa cctcttacgt gcccgatcaa tcatgaccaa
      961 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     1021 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     1081 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     1141 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     1201 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     1261 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     1321 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     1381 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     1441 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     1501 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     1561 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     1621 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     1681 tcctgcgtta tcccctgatt ctgtggataa ccgtag
//
