LOCUS       Exported                2301 bp ds-DNA     circular SYN 20-MAR-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Mar 20, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Ligation of : pYTK001.gb.xdna [1642  nt] : (#BsmBI[1024] / 
            #BsmBI[6]) to PCR(pYX122-Oxa1-GFP1-10.xdna) [651  nt] : (#BsmBI[5] /
            #BsmBI[672])
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     5..655
                     /note="note=""Fragment extracted from 
                     PCR(pYX122-Oxa1-GFP1-10.xdna) [651  nt] : (#BsmBI[5] / 
                     #BsmBI[672])"
     misc_feature    14..646
                     /label=sfGFP1-10
                     /note="note=""direct strand 
                     translation""translation=""SKGEELFTGVVPILVELDGDVNGHKFSVRGEG
                     EGDATIGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGY
                     VQERTISFKDDGKYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFNSHNVY
                     ITADKQKNGIKANFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKD
                     P"
     misc_feature    complement(662..770)
                     /label=CamR Terminator
                     /note="label=CamR Terminator - ApEinfo_fwdcolor=#66ccff - 
                     ApEinfo_revcolor=#66ccff - ApEinfo_graphicformat=arrow_data
                     {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 
                     complement(1037..1145)"
     CDS             complement(771..1430)
                     /label=CamR
                     /note="label=CamR - ApEinfo_fwdcolor=#0000FF - 
                     ApEinfo_revcolor=#0000FF - ApEinfo_graphicformat=arrow_data
                     {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 
                     complement(1146..1805)"
     promoter        complement(1431..1535)
                     /label=CamR Promoter
                     /note="label=CamR Promoter - ApEinfo_fwdcolor=#66CCFF - 
                     ApEinfo_revcolor=#66CCFF - ApEinfo_graphicformat=arrow_data
                     {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 
                     complement(1806..1910)"
     rep_origin      complement(1536..2299)
                     /direction=LEFT
                     /label=ColE1
                     /note="label=ColE1 - ApEinfo_fwdcolor=#7F7F7F - 
                     ApEinfo_revcolor=#7F7F7F - ApEinfo_graphicformat=arrow_data
                     {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 
                     complement(1911..2674)"
ORIGIN
        1 tcggtctcat atgtctaaag gtgaagaatt gtttactggt gttgttccaa ttttggttga
       61 attggatggt gatgttaatg gtcataaatt ttctgttaga ggtgaaggtg aaggtgatgc
      121 tactattggt aaattgactt tgaaatttat ttgtactact ggtaaattgc cagttccatg
      181 gccaactttg gttactactt tgacttatgg tgttcaatgt ttttctagat atccagatca
      241 tatgaaacaa catgattttt ttaaatctgc tatgccagaa ggttatgttc aagaaagaac
      301 tatttctttt aaagatgatg gtaaatataa aactagagct gttgttaaat ttgaaggtga
      361 tactttggtt aatagaattg aattgaaagg tactgatttt aaagaagatg gtaatatttt
      421 gggtcataaa ttggaatata attttaattc tcataatgtt tatattactg ctgataaaca
      481 aaaaaatggt attaaagcta attttactgt tagacataat gttgaagatg gttctgttca
      541 attggctgat cattatcaac aaaatactcc aattggtgat ggtccagttt tgttgccaga
      601 taatcattat ttgtctactc aaactgtttt gtctaaagat ccaaatggat cctgagacca
      661 gaccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg
      721 aggtcattac tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg
      781 ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca
      841 tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta
      901 taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa
      961 tcaaaactgg tgaaactcac ccagggattg gctgaaacga aaaacatatt ctcaataaac
     1021 cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt
     1081 agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc
     1141 tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc
     1201 attgccatac gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc
     1261 ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga
     1321 acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga
     1381 tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc
     1441 ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta
     1501 tggtgaaagt tggaacctct tacgtgcccg atcaatcatg accaaaatcc cttaacgtga
     1561 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     1621 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     1681 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     1741 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     1801 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     1861 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     1921 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     1981 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     2041 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     2101 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     2161 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     2221 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     2281 tgattctgtg gataaccgta g
//
