LOCUS       Exported                1691 bp ds-DNA     circular SYN 20-MAR-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Mar 20, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Ligation of : pYTK001.gb.xdna [1642  nt] : (#BsmBI[1024] / 
            #BsmBI[6]) to Sequence Window #2 [41  nt] : (#BsmBI[5] / #BsmBI[62])
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     5..45
                     /note="note=""Fragment extracted from Sequence Window #2 
                     [41  nt] : (#BsmBI[5] / #BsmBI[62])"
     misc_feature    14..38
                     /label=pSP6_Kozak
     misc_feature    complement(52..160)
                     /label=CamR Terminator
                     /note="label=CamR Terminator - ApEinfo_fwdcolor=#66ccff - 
                     ApEinfo_revcolor=#66ccff - ApEinfo_graphicformat=arrow_data
                     {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 
                     complement(1037..1145)"
     CDS             complement(161..820)
                     /label=CamR
                     /note="label=CamR - ApEinfo_fwdcolor=#0000FF - 
                     ApEinfo_revcolor=#0000FF - ApEinfo_graphicformat=arrow_data
                     {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 
                     complement(1146..1805)"
     promoter        complement(821..925)
                     /label=CamR Promoter
                     /note="label=CamR Promoter - ApEinfo_fwdcolor=#66CCFF - 
                     ApEinfo_revcolor=#66CCFF - ApEinfo_graphicformat=arrow_data
                     {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 
                     complement(1806..1910)"
     rep_origin      complement(926..1689)
                     /direction=LEFT
                     /label=ColE1
                     /note="label=ColE1 - ApEinfo_fwdcolor=#7F7F7F - 
                     ApEinfo_revcolor=#7F7F7F - ApEinfo_graphicformat=arrow_data
                     {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 
                     complement(1911..2674)"
ORIGIN
        1 tcggtctcaa acgatttagg tgacactata gaagccacta tgtgagacca gaccaataaa
       61 aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac
      121 tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg ccctgccact
      181 catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg
      241 catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc
      301 ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg
      361 tgaaactcac ccagggattg gctgaaacga aaaacatatt ctcaataaac cctttaggga
      421 aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc
      481 ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa
      541 cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac
      601 gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact
      661 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt
      721 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg
      781 atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg
      841 aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt
      901 tggaacctct tacgtgcccg atcaatcatg accaaaatcc cttaacgtga gttttcgttc
      961 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     1021 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     1081 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     1141 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     1201 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     1261 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     1321 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     1381 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     1441 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     1501 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     1561 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     1621 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     1681 gataaccgta g
//
