LOCUS       Exported                1717 bp ds-DNA     circular SYN 20-MAR-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Mar 20, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Ligation of : pYTK001.gb.xdna [1642  nt] : (#BsmBI[1024] / 
            #BsmBI[6]) to Sequence Window #2 [67  nt] : (#BsmBI[5] / #BsmBI[88])
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     5..71
                     /note="note=""Fragment extracted from Sequence Window #2 
                     [67  nt] : (#BsmBI[5] / #BsmBI[88])"
     misc_feature    14..64
                     /label=tT7
     misc_feature    complement(78..186)
                     /label=CamR Terminator
                     /note="label=CamR Terminator - ApEinfo_fwdcolor=#66ccff - 
                     ApEinfo_revcolor=#66ccff - ApEinfo_graphicformat=arrow_data
                     {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 
                     complement(1037..1145)"
     CDS             complement(187..846)
                     /label=CamR
                     /note="label=CamR - ApEinfo_fwdcolor=#0000FF - 
                     ApEinfo_revcolor=#0000FF - ApEinfo_graphicformat=arrow_data
                     {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 
                     complement(1146..1805)"
     promoter        complement(847..951)
                     /label=CamR Promoter
                     /note="label=CamR Promoter - ApEinfo_fwdcolor=#66CCFF - 
                     ApEinfo_revcolor=#66CCFF - ApEinfo_graphicformat=arrow_data
                     {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 
                     complement(1806..1910)"
     rep_origin      complement(952..1715)
                     /direction=LEFT
                     /label=ColE1
                     /note="label=ColE1 - ApEinfo_fwdcolor=#7F7F7F - 
                     ApEinfo_revcolor=#7F7F7F - ApEinfo_graphicformat=arrow_data
                     {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 
                     complement(1911..2674)"
ORIGIN
        1 tcggtctcaa tcctaactag cataacccct tggggcctct aaacgggtct tgaggggttt
       61 tttggctgtg agaccagacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa
      121 tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag cgagctcgat
      181 atcaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct
      241 gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac
      301 cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat
      361 attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg aaacgaaaaa
      421 catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc
      481 ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga
      541 aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac
      601 cagctcaccg tctttcattg ccatacgaaa ttccggatga gcattcatca ggcgggcaag
      661 aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc
      721 cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc
      781 aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt
      841 ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag
      901 tgatcttatt tcattatggt gaaagttgga acctcttacg tgcccgatca atcatgacca
      961 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     1021 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     1081 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     1141 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     1201 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     1261 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     1321 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     1381 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     1441 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     1501 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     1561 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     1621 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     1681 ttcctgcgtt atcccctgat tctgtggata accgtag
//
