LOCUS       Exported                1714 bp ds-DNA     circular SYN 20-MAR-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Mar 20, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Ligation of : pYTK001.gb.xdna [1642  nt] : (#BsmBI[1024] / 
            #BsmBI[6]) to Sequence Window #2 [64  nt] : (#BsmBI[5] / #BsmBI[85])
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     5..68
                     /note="note=""Fragment extracted from Sequence Window #2 
                     [64  nt] : (#BsmBI[5] / #BsmBI[85])"
     misc_feature    14..61
                     /label=tT7
     misc_feature    complement(75..183)
                     /label=CamR Terminator
                     /note="label=CamR Terminator - ApEinfo_fwdcolor=#66ccff - 
                     ApEinfo_revcolor=#66ccff - ApEinfo_graphicformat=arrow_data
                     {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 
                     complement(1037..1145)"
     CDS             complement(184..843)
                     /label=CamR
                     /note="label=CamR - ApEinfo_fwdcolor=#0000FF - 
                     ApEinfo_revcolor=#0000FF - ApEinfo_graphicformat=arrow_data
                     {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 
                     complement(1146..1805)"
     promoter        complement(844..948)
                     /label=CamR Promoter
                     /note="label=CamR Promoter - ApEinfo_fwdcolor=#66CCFF - 
                     ApEinfo_revcolor=#66CCFF - ApEinfo_graphicformat=arrow_data
                     {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 
                     complement(1806..1910)"
     rep_origin      complement(949..1712)
                     /direction=LEFT
                     /label=ColE1
                     /note="label=ColE1 - ApEinfo_fwdcolor=#7F7F7F - 
                     ApEinfo_revcolor=#7F7F7F - ApEinfo_graphicformat=arrow_data
                     {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 
                     complement(1911..2674)"
ORIGIN
        1 tcggtctcat ggcctagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt
       61 ggctgtgaga ccagaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc
      121 agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga gctcgatatc
      181 aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc
      241 gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt
      301 gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt
      361 ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaaa cgaaaaacat
      421 attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg
      481 cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa
      541 cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag
      601 ctcaccgtct ttcattgcca tacgaaattc cggatgagca ttcatcaggc gggcaagaat
      661 gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt
      721 aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa
      781 atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc
      841 cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga
      901 tcttatttca ttatggtgaa agttggaacc tcttacgtgc ccgatcaatc atgaccaaaa
      961 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     1021 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     1081 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     1141 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     1201 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     1261 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     1321 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     1381 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     1441 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     1501 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     1561 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     1621 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     1681 ctgcgttatc ccctgattct gtggataacc gtag
//
