LOCUS       Exported                2940 bp ds-DNA     circular SYN 20-MAR-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Mar 20, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Ligation of : pYTK001.gb.xdna [1642  nt] : (#BsmBI[1024] / 
            #BsmBI[6]) to PCR(LacI.xdna) [1290  nt] : (#BsmBI[5] / #BsmBI[1311])
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     5..1294
                     /note="note=""Fragment extracted from PCR(LacI.xdna) [1290 
                     nt] : (#BsmBI[5] / #BsmBI[1311])"
     misc_feature    215..1053
                     /label=LacI [*]
     misc_feature    1054
                     /label=LacI [*] [5']
     misc_feature    1056..1174
                     /label=LacI [*] [3']
     misc_feature    complement(1301..1409)
                     /label=CamR Terminator
                     /note="label=CamR Terminator - ApEinfo_fwdcolor=#66ccff - 
                     ApEinfo_revcolor=#66ccff - ApEinfo_graphicformat=arrow_data
                     {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 
                     complement(1037..1145)"
     CDS             complement(1410..2069)
                     /label=CamR
                     /note="label=CamR - ApEinfo_fwdcolor=#0000FF - 
                     ApEinfo_revcolor=#0000FF - ApEinfo_graphicformat=arrow_data
                     {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 
                     complement(1146..1805)"
     promoter        complement(2070..2174)
                     /label=CamR Promoter
                     /note="label=CamR Promoter - ApEinfo_fwdcolor=#66CCFF - 
                     ApEinfo_revcolor=#66CCFF - ApEinfo_graphicformat=arrow_data
                     {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 
                     complement(1806..1910)"
     rep_origin      complement(2175..2938)
                     /direction=LEFT
                     /label=ColE1
                     /note="label=ColE1 - ApEinfo_fwdcolor=#7F7F7F - 
                     ApEinfo_revcolor=#7F7F7F - ApEinfo_graphicformat=arrow_data
                     {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 
                     complement(1911..2674)"
ORIGIN
        1 tcggtctcat acagacacca tcgaatggcg caaaaccttt cgcggtatgg catgatagcg
       61 cccggaagag agtcaattca gggtggtgaa tgtgaaacca gtaacgttat acgatgtcgc
      121 agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt
      181 ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt acattcccaa
      241 ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg attggcgttg ccacctccag
      301 tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact
      361 gggtgccagc gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc
      421 ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc attaactatc cgctggatga
      481 ccaggatgcc attgctgtgg aagctgcctg cactaatgtt ccggcgttat ttcttgatgt
      541 ctctgaccag acacccatca acagtattat tttctcccat gaagacggta cgcgactggg
      601 cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag
      661 ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa tatctcactc gcaatcaaat
      721 tcagccgata gcggaacggg aaggcgactg gagtgccatg tccggttttc aacaaaccat
      781 gcaaatgctg aatgagggca tcgttcccac tgcgatgctg gttgccaacg atcagatggc
      841 gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt
      901 agtgggatac gacgataccg aagacagctc atgttatatc ccgccgttaa ccaccatcaa
      961 acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg
     1021 ccaggcggtg aagggcaatc agctgttgcc cgtttcactg gtgaaaagaa aaaccaccct
     1081 ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     1141 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtaagttagc
     1201 tcactcatta ggcaccggga tctcgaccga tgcccttgag agccttcaac ccagtcagct
     1261 ccttccggtg ggcgcggggc atgactaccg atgagaccag accaataaaa aacgcccggc
     1321 ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca
     1381 acaggagtcc aagcgagctc gatatcaaat tacgccccgc cctgccactc atcgcagtac
     1441 tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc
     1501 tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa
     1561 acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc
     1621 cagggattgg ctgaaacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg
     1681 ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg
     1741 tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa
     1801 gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg aaattccgga
     1861 tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt
     1921 ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat
     1981 tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg
     2041 gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat
     2101 aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt
     2161 acgtgcccga tcaatcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     2221 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     2281 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     2341 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     2401 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     2461 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     2521 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     2581 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     2641 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     2701 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     2761 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     2821 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     2881 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtag
//
