LOCUS       Exported                1687 bp ds-DNA     circular SYN 20-MAR-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Mar 20, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Ligation of : pYTK001.gb [1642  nt] : (#BsmBI[1024] / #BsmBI[6]) to 
            Sequence Window #3 [37  nt] : (#BsmBI[5] / #BsmBI[58])
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    14..34
                     /label=6xHis
     misc_feature    complement(48..156)
                     /label=CamR Terminator
                     /note="label=CamR Terminator - ApEinfo_fwdcolor=#66ccff - 
                     ApEinfo_revcolor=#66ccff - ApEinfo_graphicformat=arrow_data
                     {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 
                     complement(1037..1145)"
     CDS             complement(157..816)
                     /label=CamR
                     /note="label=CamR - ApEinfo_fwdcolor=#0000FF - 
                     ApEinfo_revcolor=#0000FF - ApEinfo_graphicformat=arrow_data
                     {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 
                     complement(1146..1805)"
     promoter        complement(817..921)
                     /label=CamR Promoter
                     /note="label=CamR Promoter - ApEinfo_fwdcolor=#66CCFF - 
                     ApEinfo_revcolor=#66CCFF - ApEinfo_graphicformat=arrow_data
                     {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 
                     complement(1806..1910)"
     rep_origin      complement(922..1685)
                     /direction=LEFT
                     /label=ColE1
                     /note="label=ColE1 - ApEinfo_fwdcolor=#7F7F7F - 
                     ApEinfo_revcolor=#7F7F7F - ApEinfo_graphicformat=arrow_data
                     {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 
                     complement(1911..2674)"
ORIGIN
        1 tcggtctcaa tcccatcatc accatcatca ctaatggctg agaccagacc aataaaaaac
       61 gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga
      121 tctatcaaca ggagtccaag cgagctcgat atcaaattac gccccgccct gccactcatc
      181 gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg
      241 atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat
      301 ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa
      361 actcacccag ggattggctg aaacgaaaaa catattctca ataaaccctt tagggaaata
      421 ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa
      481 atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt
      541 gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg ccatacgaaa
      601 ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg
      661 cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata
      721 ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat
      781 atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa
      841 tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga
      901 acctcttacg tgcccgatca atcatgacca aaatccctta acgtgagttt tcgttccact
      961 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     1021 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     1081 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     1141 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     1201 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     1261 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     1321 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     1381 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     1441 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     1501 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     1561 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     1621 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     1681 accgtag
//
