LOCUS       Gonzalez_Y9M_2_9        6087 bp    DNA        circular     20-MAR-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  . .
COMMENT     Annotated with pLannotate v1.2.0
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             complement(5097..5833)
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             complement(5834..5957)
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /locus_tag="AmpR(1)"
                     /label="AmpR(1)"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     rep_origin      complement(4338..4926)
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
                     /locus_tag="ori"
                     /label="ori"
                     /ApEinfo_label="ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    143..2008
                     /locus_tag="Rep2"
                     /label="Rep2"
                     /ApEinfo_label="Rep2"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2025..4234
                     /locus_tag="Cap9 + null mutation"
                     /label="Cap9 + null mutation"
                     /ApEinfo_label="Cap9 + null mutation"
                     /ApEinfo_fwdcolor="#ffff80"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    3780..3799
                     /locus_tag="VR8 null mutation"
                     /label="VR8 null mutation"
                     /ApEinfo_label="VR8 null mutation"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     promoter        complement(5958..6062)
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /locus_tag="AmpR promoter"
                     /label="AmpR promoter"
                     /ApEinfo_label="AmpR promoter"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 gatatcctta aggtcctgta ttagaggtca cgtgagtgtt ttgcgacatt ttgcgacacc
       61 atgtggtcac gctgggtatt taagcccgag tgagcacgca gggtctccat tttgaagcgg
      121 gaggtttgaa cgcgcagcca ccacgccggg gttttacgag attgtgatta aggtccccag
      181 cgaccttgac gagcatctgc ccggcatttc tgacagcttt gtgaactggg tggccgagaa
      241 ggaatgggag ttgccgccag attctgacat ggatctgaat ctgattgagc aggcacccct
      301 gaccgtggcc gagaagctgc agcgcgactt tctgacggaa tggcgccgtg tgagtaaggc
      361 cccggaggcc cttttctttg tgcaatttga gaagggagag agctacttcc acatgcacgt
      421 gctcgtggaa accaccgggg tgaaatccat ggttttggga cgtttcctga gtcagattcg
      481 cgaaaaactg attcagagaa tttaccgcgg gatcgagccg actttgccaa actggttcgc
      541 ggtcacaaag accagaaatg gcgccggagg cgggaacaag gtggtggatg agtgctacat
      601 ccccaattac ttgctcccca aaacccagcc tgagctccag tgggcgtgga ctaatatgga
      661 acagtattta agcgcctgtt tgaatctcac ggagcgtaaa cggttggtgg cgcagcatct
      721 gacgcacgtg tcgcagacgc aggagcagaa caaagagaat cagaatccca attctgatgc
      781 gccggtgatc agatcaaaaa cttcagccag gtacatggag ctggtcgggt ggctcgtgga
      841 caaggggatt acctcggaga agcagtggat ccaggaggac caggcctcat acatctcctt
      901 caatgcggcc tccaactcgc ggtcccaaat caaggctgcc ttggacaatg cgggaaagat
      961 tatgagcctg actaaaaccg cccccgacta cctggtgggc cagcagcccg tggaggacat
     1021 ttccagcaat cggatttata aaattttgga actaaacggg tacgatcccc aatatgcggc
     1081 ttccgtcttt ctgggatggg ccacgaaaaa gttcggcaag aggaacacca tctggctgtt
     1141 tgggcctgca actaccggga agaccaacat cgcggaggcc atagcccaca ctgtgccctt
     1201 ctacgggtgc gtaaactgga ccaatgagaa ctttcccttc aacgactgtg tcgacaagat
     1261 ggtgatctgg tgggaggagg ggaagatgac cgccaaggtc gtggagtcgg ccaaagccat
     1321 tctcggagga agcaaggtgc gcgtggacca gaaatgcaag tcctcggccc agatagaccc
     1381 gactcccgtg atcgtcacct ccaacaccaa catgtgcgcc gtgattgacg ggaactcaac
     1441 gaccttcgaa caccagcagc cgttgcaaga ccggatgttc aaatttgaac tcacccgccg
     1501 tctggatcat gactttggga aggtcaccaa gcaggaagtc aaagactttt tccggtgggc
     1561 aaaggatcac gtggttgagg tggagcatga attctacgtc aaaaagggtg gagccaagaa
     1621 aagacccgcc cccagtgacg cagatataag tgagcccaaa cgggtgcgcg agtcagttgc
     1681 gcagccatcg acgtcagacg cggaagcttc gatcaactac gcagacaggt accaaaacaa
     1741 atgttctcgt cacgtgggca tgaatctgat gctgtttccc tgcagacaat gcgagagaat
     1801 gaatcagaat tcaaatatct gcttcactca cggacagaaa gactgtttag agtgctttcc
     1861 cgtgtcagaa tctcaacccg tttctgtcgt caaaaaggcg tatcagaaac tgtgctacat
     1921 tcatcatatc atgggaaagg tgccagacgc ttgcactgcc tgcgatctgg tcaatgtgga
     1981 tttggatgac tgcatctttg aacaataaat gatttaaatc aggtatggct gccgatggtt
     2041 atcttccaga ttggctcgag gacaacctta gtgaaggaat tcgcgagtgg tgggctttga
     2101 aacctggagc ccctcaaccc aaggcaaatc aacaacatca agacaacgct cgaggtcttg
     2161 tgcttccggg ttacaaatac cttggacccg gcaacggact cgacaagggg gagccggtca
     2221 acgcagcaga cgcggcggcc ctcgagcacg acaaggccta cgaccagcag ctcaaggccg
     2281 gagacaaccc gtacctcaag tacaaccacg ccgacgccga gttccaggag cggctcaaag
     2341 aagatacgtc ttttgggggc aacctcgggc gagcagtctt ccaggccaaa aagaggcttc
     2401 ttgaacctct tggtctggtt gaggaagcgg ctaagacggc tcctggaaag aagaggcctg
     2461 tagagcagtc tcctcaggaa ccggactcct ccgcgggtat tggcaaatcg ggtgcacagc
     2521 ccgctaaaaa gagactcaat ttcggtcaga ctggcgacac agagtcagtc ccagaccctc
     2581 aaccaatcgg agaacctccc gcagccccct caggtgtggg atctcttaca atggcttcag
     2641 gtggtggcgc accagtggca gacaataacg aaggtgccga tggagtgggt agttcctcgg
     2701 gaaattggca ttgcgattcc caatggctgg gggacagagt catcaccacc agcacccgaa
     2761 cctgggccct gcccacctac aacaatcacc tctacaagca aatctccaac agcacatctg
     2821 gaggatcttc aaatgacaac gcctacttcg gctacagcac cccctggggg tattttgact
     2881 tcaacagatt ccactgccac ttctcaccac gtgactggca gcgactcatc aacaacaact
     2941 ggggattccg gcctaagcga ctcaacttca agctcttcaa cattcaggtc aaagaggtta
     3001 cggacaacaa tggagtcaag accatcgcca ataaccttac cagcacggtc caggtcttca
     3061 cggactcaga ctatcagctc ccgtacgtgc tcgggtcggc tcacgagggc tgcctcccgc
     3121 cgttcccagc ggacgttttc atgattcctc agtacgggta tctgacgctt aatgatggaa
     3181 gccaggccgt gggtcgttcg tccttttact gcctggaata tttcccgtcg caaatgctaa
     3241 gaacgggtaa caacttccag ttcagctacg agtttgagaa cgtacctttc catagcagct
     3301 acgctcacag ccaaagcctg gaccgactaa tgaatccact catcgaccaa tacttgtact
     3361 atctctcaaa gactattaac ggttctggac agaatcaaca aacgctaaaa ttcagtgtgg
     3421 ccggacccag caacatggct gtccagggaa gaaactacat acctggaccc agctaccgac
     3481 aacaacgtgt ctcaaccact gtgactcaaa acaacaacag cgaatttgct tggcctggag
     3541 cttcttcttg ggctctcaat ggacgtaata gcttgatgaa tcctggacct gctatggcca
     3601 gccacaaaga aggagaggac cgtttctttc ctttgtctgg atctttaatt tttggcaaac
     3661 aaggaactgg aagagacaac gtggatgcgg acaaagtcat gataaccaac gaagaagaaa
     3721 ttaaaactac taacccggta gcaacggagt cctatggaca agtggccaca aaccaccagt
     3781 agctgatctt agtgagtaga ccggctgggt tcaaaaccaa ggaatacttc cgggtatggt
     3841 ttggcaggac agagatgtgt acctgcaagg acccatttgg gccaaaattc ctcacacgga
     3901 cggcaacttt cacccttctc cgctgatggg agggtttgga atgaagcacc cgcctcctca
     3961 gatcctcatc aaaaacacac ctgtacctgc ggatcctcca acggccttca acaaggacaa
     4021 gctgaactct ttcatcaccc agtattctac tggccaagtc agcgtggaga tcgagtggga
     4081 gctgcagaag gaaaacagca agcgctggaa cccggagatc cagtacactt ccaactatta
     4141 caagtctaat aatgttgaat ttgctgttaa tactgaaggt gtatatagtg aaccccgccc
     4201 cattggcacc agatacctga ctcgtaatct gtaattgctt gttaatcaat aaaccgttta
     4261 attcgtttca gttgaacttt ggtctagaga tatcagcaaa aggccaggaa ccgtaaaaag
     4321 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     4381 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     4441 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     4501 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     4561 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     4621 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     4681 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     4741 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     4801 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     4861 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     4921 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     4981 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     5041 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     5101 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     5161 gcctggctcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     5221 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     5281 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     5341 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     5401 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     5461 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     5521 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     5581 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     5641 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     5701 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     5761 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     5821 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     5881 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     5941 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     6001 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     6061 cgcacatttc cccgaaaagt gccacct   
//
