LOCUS       PB_Halo_Hsf2_IRES_Neo   9411 bp ds-DNA     circular     27-MAY-2022
DEFINITION  .
KEYWORDS    "Sequence" "Box" "creator:luye" "marker:AmpR, NeoR/KanR"
FEATURES             Location/Qualifiers
     CDS             complement(650..718)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     promoter        complement(719..823)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     rep_origin      complement(849..1303)
                     /label="f1 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     1444..1460
                     /label="M13 fwd"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
     misc_feature    2087..2610
                     /label="EF1a prom"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     promoter        2105..2316
                     /label="EF-1α core promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     LTR             2329..2597
                     /label="5' LTR (truncated)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          2538..2557
                     /label="oST085"
                     /note="sequence: GACCCTGCTTGCTCAACTCT"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    2619..2619
                     /label="EX-MCS"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             2629..2697
                     /label="3xFLAG"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             2629..5196
                     /label="Translation 2629-5196"
                     /translation="MDYKDHDGDYKDHDIDYKDDDDKLGSMAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIGSEIARWLSTLEISGEPTTEDLYFQSDNSRMKQSSNVPAFLSKLWTLVEETHTNEFITWSQNGQSFLVLDEQRFAKEILPKYFKHNNMASFVRQLNMYGFRKVVHIESGIIKQERDGPVEFQHPYFKQGQDDLLENIKRKVSSSKPEENKIRQEDLTKIISSAQKVQIKQETIESRLSELKSENESLWKEVSELRAKHAQQQQVIRKIVQFIVTLVQNNQLVSLKRKRPLLLNTNGAPKKNLYQHIVKEPTDNHHHKVPHSRTEGLKSRERISDDIIIYDVTDDNVDEENIPVIPETNEDVVVDSSNQYPDIVIVEDDNEDEYAPVIQSGEQSEPAREPLRVGSAGSSSPLMSSAVQLNGSSSLTSEDPVTMMDSILNDNINLLGKVELLDYLDSIDCSLEDFQAMLSGRQFSIDPDLLVDSENKGLEATKSSVVQHVSEEGRKSKSKPDKQLIQYTAFPLLAFLDGNSASAIEQGSTTASSEVVPSVDKPIEVDELLDSSLDPEPTQSKLVRLEPLTEAEASEATLFYLCELAPAPLDSDMPLLDS*"
     CDS             2707..3597
                     /label="HaloTag"
                     /ApEinfo_revcolor="#333399"
                     /ApEinfo_fwdcolor="#333399"
     primer          2721..2741
                     /label="oST450"
                     /note="sequence: TACTGGCTTTCCATTCGACCC"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          2821..2859
                     /label="oST779"
                     /note="sequence: CACGGTAACCCGACCTCCTCCTACGTGTGGCGCAACATC"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          3488..3507
                     /label="oST183"
                     /note="sequence: ACTGCAAGGCTGTGGACATC"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(3512..3530)
                     /label="oST451"
                     /note="sequence: TGCAGCAGATTCAGACCCG"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          3523..3542
                     /label="Halo-H2.qPCR.fwd"
                     /note="sequence: CTGCTGCAAGAAGACAACCC"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             3610..3630
                     /label="TEV protease site"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     primer          3637..3664
                     /label="Maybe primer 2"
                     /note="sequence: TCTAGAatgaagcagagttccaacgtgc"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     exon            3642..3735
                     /label="exon"
                     /ApEinfo_revcolor="#708090"
                     /ApEinfo_fwdcolor="#708090"
     gene            3642..5196
                     /label="Hsf2"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    3643..3975
                     /label="HSF2DBD"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             3643..5196
                     /label="Hsf2"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     CDS             3643..5196
                     /label="Translation 3643-5196"
                     /translation="MKQSSNVPAFLSKLWTLVEETHTNEFITWSQNGQSFLVLDEQRFAKEILPKYFKHNNMASFVRQLNMYGFRKVVHIESGIIKQERDGPVEFQHPYFKQGQDDLLENIKRKVSSSKPEENKIRQEDLTKIISSAQKVQIKQETIESRLSELKSENESLWKEVSELRAKHAQQQQVIRKIVQFIVTLVQNNQLVSLKRKRPLLLNTNGAPKKNLYQHIVKEPTDNHHHKVPHSRTEGLKSRERISDDIIIYDVTDDNVDEENIPVIPETNEDVVVDSSNQYPDIVIVEDDNEDEYAPVIQSGEQSEPAREPLRVGSAGSSSPLMSSAVQLNGSSSLTSEDPVTMMDSILNDNINLLGKVELLDYLDSIDCSLEDFQAMLSGRQFSIDPDLLVDSENKGLEATKSSVVQHVSEEGRKSKSKPDKQLIQYTAFPLLAFLDGNSASAIEQGSTTASSEVVPSVDKPIEVDELLDSSLDPEPTQSKLVRLEPLTEAEASEATLFYLCELAPAPLDSDMPLLDS*"
     primer          complement(3684..3703)
                     /label="Halo-H2.qPCR.rev"
                     /note="sequence: tttcctccacaagcgtccac"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     exon            3736..3844
                     /label="exon"
                     /ApEinfo_revcolor="#708090"
                     /ApEinfo_fwdcolor="#708090"
     exon            3845..3972
                     /label="exon"
                     /ApEinfo_revcolor="#708090"
                     /ApEinfo_fwdcolor="#708090"
     misc_feature    3964..4008
                     /label="NLS1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     exon            3973..4097
                     /label="exon"
                     /ApEinfo_revcolor="#708090"
                     /ApEinfo_fwdcolor="#708090"
     misc_feature    3997..4218
                     /label="HR-A/B "
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          4009..4034
                     /label="oST204"
                     /note="sequence: caggaagatttaacaaaaattattag"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     exon            4098..4173
                     /label="exon"
                     /ApEinfo_revcolor="#708090"
                     /ApEinfo_fwdcolor="#708090"
     exon            4174..4235
                     /label="exon"
                     /ApEinfo_revcolor="#708090"
                     /ApEinfo_fwdcolor="#708090"
     misc_feature    4225..4269
                     /label="NLS2"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     exon            4236..4323
                     /label="exon"
                     /ApEinfo_revcolor="#708090"
                     /ApEinfo_fwdcolor="#708090"
     exon            4324..4472
                     /label="exon"
                     /ApEinfo_revcolor="#708090"
                     /ApEinfo_fwdcolor="#708090"
     exon            4473..4709
                     /label="exon"
                     /ApEinfo_revcolor="#708090"
                     /ApEinfo_fwdcolor="#708090"
     exon            4710..4815
                     /label="exon"
                     /ApEinfo_revcolor="#708090"
                     /ApEinfo_fwdcolor="#708090"
     exon            4816..4900
                     /label="exon"
                     /ApEinfo_revcolor="#708090"
                     /ApEinfo_fwdcolor="#708090"
     STS             4889..5196
                     /label="Hsf2"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     exon            4901..5196
                     /label="exon"
                     /ApEinfo_revcolor="#708090"
                     /ApEinfo_fwdcolor="#708090"
     STS             4976..5094
                     /label="Hsf2"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    5199..5204
                     /label="EX-MCS"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(5205..5220)
                     /label="oST614"
                     /note="sequence: GGGGAGGGAGAGGGGC"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     RBS             5205..5778
                     /label="IRES"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(5287..5306)
                     /label="oST086"
                     /note="sequence: AAGACGGCAATATGGTGGAA"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             5787..6581
                     /label="NeoR/KanR"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(6560..6599)
                     /label="oST660"
                     /note="sequence: CAGAGGTTGATTGTCGACTCAGAAGAACTCGTCAAGAAGG"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(6560..6599)
                     /label="oST660"
                     /note="sequence: CAGAGGTTGATTGTCGACTCAGAAGAACTCGTCAAGAAGG"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          6582..6607
                     /label="oST661 copy"
                     /note="sequence: GTCGACAATCAACCTCTGGATTACAA"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     polyA_signal    6703..6824
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     complement(8109..8125)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
     protein_bind    8133..8149
                     /label="lac operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     promoter        complement(8157..8163)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        complement(8164..8181)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        complement(8182..8187)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind    8202..8223
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     rep_origin      complement(8511..9098)
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             complement(9269..649)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
ORIGIN
        1 ACCCACGCTC ACCGGTTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC
       61 GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG
      121 CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA
      181 TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA
      241 GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA
      301 TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA
      361 ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA
      421 AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG
      481 ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG
      541 GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG
      601 CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG
      661 GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC
      721 TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA
      781 TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG
      841 TGCCACCTAA ATTGTAAGCG TTAATATTTT GTTAAAATTC GCGTTAAATT TTTGTTAAAT
      901 CAGCTCATTT TTTAACCAAT AGGCCGAAAT CGGCAAAATC CCTTATAAAT CAAAAGAATA
      961 GACCGAGATA GGGTTGAGTG TTGTTCCAGT TTGGAACAAG AGTCCACTAT TAAAGAACGT
     1021 GGACTCCAAC GTCAAAGGCG AAAAACCGTC TATCAGGGCG ATGGCCCACT ACGTGAACCA
     1081 TCACCCTAAT CAAGTTTTTT GGGGTCGAGG TGCCGTAAAG CACTAAATCG GAACCCTAAA
     1141 GGGAGCCCCC GATTTAGAGC TTGACGGGGA AAGCCGGCGA ACGTGGCGAG AAAGGAAGGG
     1201 AAGAAAGCGA AAGGAGCGGG CGCTAGGGCG CTGGCAAGTG TAGCGGTCAC GCTGCGCGTA
     1261 ACCACCACAC CCGCCGCGCT TAATGCGCCG CTACAGGGCG CGTCCCATTC GCCATTCAGG
     1321 CTGCGCAACT GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG CCAGCTGGCG
     1381 AAAGGGGGAT GTGCTGCAAG GCGATTAAGT TGGGTAACGC CAGGGTTTCC CAGTCACGAC
     1441 GTTGTAAAAC GACGGCCAGT GAGCGCGCCT CGTTCATTCA CGTTTTTGAA CCCGTGGAGG
     1501 ACGGGCAGAC TCGCGGTGCA AATGTGTTTT ACAGCGTGAT GGAGCAGATG AAGATGCTCG
     1561 ACACGCTGCA GAACACGCAG CTAGATTAAC CCTAGAAAGA TAATCATATT GTGACGTACG
     1621 TTAAAGATAA TCATGTGTAA AATTGACGCA TGTGTTTTAT CGGTCTGTAT ATCGAGGTTT
     1681 ATTTATTAAT TTGAATAGAT ATTAAGTTTT ATTATATTTA CACTTACATA CTAATAATAA
     1741 ATTCAACAAA CAATTTATTT ATGTTTATTT ATTTATTAAA AAAAACAAAA ACTCAAAATT
     1801 TCTTCTATAA AGTAACAAAA CTTTTATGAG GGACAGCCCC CCCCCAAAGC CCCCAGGGAT
     1861 GTAATTACGT CCCTCCCCCG CTAGGGGGCA GCAGCGAGCC GCCCGGGGCT CCGCTCCGGT
     1921 CCGGCGCTCC CCCCGCATCC CCGAGCCGGC AGCGTGCGGG GACAGCCCGG GCACGGGGAA
     1981 GGTGGCACGG GATCGCTTTC CTCTGAACGC TTCTCGCTGC TCTTTGAGCC TGCAGACACC
     2041 TGGGGGGATA CGGGGAAAAG GCCTCCACGG CCAAGGATCT GCGATCGCTC CGGTGCCCGT
     2101 CAGTGGGCAG AGCGCACATC GCCCACAGTC CCCGAGAAGT TGGGGGGAGG GGTCGGCAAT
     2161 TGAACGGGTG CCTAGAGAAG GTGGCGCGGG GTAAACTGGG AAAGTGATGT CGTGTACTGG
     2221 CTCCGCCTTT TTCCCGAGGG TGGGGGAGAA CCGTATATAA GTGCAGTAGT CGCCGTGAAC
     2281 GTTCTTTTTC GCAACGGGTT TGCCGCCAGA ACACAGCTGA AGCTTCGAGG GGCTCGCATC
     2341 TCTCCTTCAC GCGCCCGCCG CCCTACCTGA GGCCGCCATC CACGCCGGTT GAGTCGCGTT
     2401 CTGCCGCCTC CCGCCTGTGG TGCCTCCTGA ACTGCGTCCG CCGTCTAGGT AAGTTTAAAG
     2461 CTCAGGTCGA GACCGGGCCT TTGTCCGGCG CTCCCTTGGA GCCTACCTAG ACTCAGCCGG
     2521 CTCTCCACGC TTTGCCTGAC CCTGCTTGCT CAACTCTACG TCTTTGTTTC GTTTTCTGTT
     2581 CTGCGCCGTT ACAGATCCAA GCTGTGACCG GCGCCTACTC TAGCCACCAT GGACTACAAA
     2641 GACCATGACG GTGATTATAA AGATCATGAC ATCGATTACA AGGATGACGA TGACAAGCTT
     2701 GGCTCCATGG CAGAAATCGG TACTGGCTTT CCATTCGACC CCCATTATGT GGAAGTCCTG
     2761 GGCGAGCGCA TGCACTACGT CGATGTTGGT CCGCGCGATG GCACCCCTGT GCTGTTCCTG
     2821 CACGGTAACC CGACCTCCTC CTACGTGTGG CGCAACATCA TCCCGCATGT TGCACCGACC
     2881 CATCGCTGCA TTGCTCCAGA CCTGATCGGT ATGGGCAAAT CCGACAAACC AGACCTGGGT
     2941 TATTTCTTCG ACGACCACGT CCGCTTCATG GATGCCTTCA TCGAAGCCCT GGGTCTGGAA
     3001 GAGGTCGTCC TGGTCATTCA CGACTGGGGC TCCGCTCTGG GTTTCCACTG GGCCAAGCGC
     3061 AATCCAGAGC GCGTCAAAGG TATTGCATTT ATGGAGTTCA TCCGCCCTAT CCCGACCTGG
     3121 GACGAATGGC CAGAATTTGC CCGCGAGACC TTCCAGGCCT TCCGCACCAC CGACGTCGGC
     3181 CGCAAGCTGA TCATCGATCA GAACGTTTTT ATCGAGGGTA CGCTGCCGAT GGGTGTCGTC
     3241 CGCCCGCTGA CTGAAGTCGA GATGGACCAT TACCGCGAGC CGTTCCTGAA TCCTGTTGAC
     3301 CGCGAGCCAC TGTGGCGCTT CCCAAACGAG CTGCCAATCG CCGGTGAGCC AGCGAACATC
     3361 GTCGCGCTGG TCGAAGAATA CATGGACTGG CTGCACCAGT CCCCTGTCCC GAAGCTGCTG
     3421 TTCTGGGGCA CCCCAGGCGT TCTGATCCCA CCGGCCGAAG CCGCTCGCCT GGCCAAAAGC
     3481 CTGCCTAACT GCAAGGCTGT GGACATCGGC CCGGGTCTGA ATCTGCTGCA AGAAGACAAC
     3541 CCGGACCTGA TCGGCAGCGA GATCGCGCGC TGGCTGTCGA CGCTCGAGAT TTCCGGCGAG
     3601 CCAACCACTG AGGATCTGTA CTTTCAGAGC GATAACTCTA GAatgaagca gagttccaac
     3661 gtgccggctt tcctcagcaa gctgtggacg cttgtggagg aaacccacac caacgagttc
     3721 atcacctgga gtcagaatgg acaaagtttt ctggtcttgg atgagcaaag atttgcaaag
     3781 gaaattcttc ctaagtactt caaacacaat aacatggcga gctttgtgag acaactaaat
     3841 atgtatggct tccgaaaagt agtgcatatc gaatctggaa ttatcaaaca ggaaagagat
     3901 ggccctgttg aatttcagca tccttatttc aagcaaggcc aggatgacct gttggagaac
     3961 attaaaagga aggtttcatc ttcaaaacca gaggaaaata aaattcgtca ggaagattta
     4021 acaaaaatta ttagtagtgc tcagaaggtt caaataaaac aagaaactat tgagtccagg
     4081 ctttcagaat taaaaagtga gaatgaatcc ctttggaagg aggtgtcaga actaagagca
     4141 aagcatgccc agcagcaaca agttattcgg aagattgtcc agtttattgt tacattggtt
     4201 cagaataatc aacttgtgag tttaaaacgt aaaaggcctc tacttctaaa cacaaatgga
     4261 gccccaaaga agaatctata tcagcacata gtcaaagaac caactgataa tcaccatcat
     4321 aaagttccac acagcaggac tgaaggttta aagtcaagag aacggatttc agatgacata
     4381 attatttatg atgttactga cgataatgtg gatgaagaaa atattccagt tattccagaa
     4441 acaaatgagg atgttgtagt ggattcctcc aaccagtatc ctgacattgt cattgttgaa
     4501 gatgacaacg aggatgagta tgctcctgtc attcagagtg gagagcagag tgaaccagcc
     4561 agagaaccct tacgtgtggg gagtgctggc agcagcagcc ctctcatgtc tagtgctgtc
     4621 cagctaaacg gctcctccag tctgacctca gaagaccctg tgaccatgat ggactccatt
     4681 ctgaatgaca acattaacct gttaggaaag gttgagctgt tggattacct tgacagtatt
     4741 gattgcagtt tagaggactt ccaagctatg ctctcaggaa gacagtttag catagaccca
     4801 gatcttctgg ttgattctga gaataaggga ctagaagcta ccaagagcag tgttgttcaa
     4861 catgtgtcag aagagggaag aaaatctaaa tccaagccag acaaacaact tatccagtat
     4921 actgcctttc cacttcttgc attcctggat gggaactctg catctgctat tgaacagggg
     4981 agtacaactg catcgtcaga agttgtgcct tctgtagata aacccataga agtcgatgag
     5041 ctcctggata gcagcctgga tccagaaccg acccagagta agcttgtccg cctggaacca
     5101 ttgactgaag cggaagctag tgaagccaca ctcttctatt tatgtgaact tgctcctgca
     5161 cctctggata gtgatatgcc gcttttagat agttaaGCGG CCGCGCCCCT CTCCCTCCCC
     5221 CCCCCCTAAC GTTACTGGCC GAAGCCGCTT GGAATAAGGC CGGTGTGCGT TTGTCTATAT
     5281 GTTATTTTCC ACCATATTGC CGTCTTTTGG CAATGTGAGG GCCCGGAAAC CTGGCCCTGT
     5341 CTTCTTGACG AGCATTCCTA GGGGTCTTTC CCCTCTCGCC AAAGGAATGC AAGGTCTGTT
     5401 GAATGTCGTG AAGGAAGCAG TTCCTCTGGA AGCTTCTTGA AGACAAACAA CGTCTGTAGC
     5461 GACCCTTTGC AGGCAGCGGA ACCCCCCACC TGGCGACAGG TGCCTCTGCG GCCAAAAGCC
     5521 ACGTGTATAA GATACACCTG CAAAGGCGGC ACAACCCCAG TGCCACGTTG TGAGTTGGAT
     5581 AGTTGTGGAA AGAGTCAAAT GGCTCTCCTC AAGCGTATTC AACAAGGGGC TGAAGGATGC
     5641 CCAGAAGGTA CCCCATTGTA TGGGATCTGA TCTGGGGCCT CGGTGCACAT GCTTTACATG
     5701 TGTTTAGTCG AGGTTAAAAA AACGTCTAGG CCCCCCGAAC CACGGGGACG TGGTTTTCCT
     5761 TTGAAAAACA CGATAATACC TCCGGAATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC
     5821 CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT
     5881 CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG
     5941 ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCG
     6001 CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC
     6061 TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA
     6121 AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC
     6181 CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC
     6241 TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG
     6301 CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT
     6361 GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC
     6421 TGGGTGTGGC GGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC
     6481 TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC
     6541 AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG AGTCGACAAT CAACCTCTGG
     6601 ATTACAAAAT TTGTGAAAGA TTGACTGGTA TTCTTAACTA TGTTGCTCCT TTTACGCTAT
     6661 GTGGATACGC TGCTTTAATG CCTTTGTATC ATGCGTTAAC TAAACTTGTT TATTGCAGCT
     6721 TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA
     6781 CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGGAATTGA
     6841 CTCAAATGAT GTCAATTAGT CTATCAGAAG CTCATCTGGT CTCCCTTCCG GGGGACAAGA
     6901 CATCCCTGTT TAATATTTAA ACAGCAGTGT TCCCAAACTG GGTTCTTATA TCCCTTGCTC
     6961 TGGTCAACCA GGTTGCAGGG TTTCCTGTCC TCACAGGAAC GAAGTCCCTA AAGAAACAGT
     7021 GGCAGCCAGG TTTAGCCCCG GAATTGACTG GATTCCTTTT TTAGGGCCCA TTGGTATGGC
     7081 TTTTTCCCCG TATCCCCCCA GGTGTCTGCA GGCTCAAAGA GCAGCGAGAA GCGTTCAGAG
     7141 GAAAGCGATC CCGTGCCACC TTCCCCGTGC CCGGGCTGTC CCCGCACGCT GCCGGCTCGG
     7201 GGATGCGGGG GGAGCGCCGG ACCGGAGCGG AGCCCCGGGC GGCTCGCTGC TGCCCCCTAG
     7261 CGGGGGAGGG ACGTAATTAC ATCCCTGGGG GCTTTGGGGG GGGGCTGTCC CTGATATCTA
     7321 TAACAAGAAA TATATATATA ATAAGTTATC ACGTAAGTAG AACATGAAAT AACAATATAA
     7381 TTATCGTATG AGTTAATCTT AAAGTCACGT AAAAGATAAT CATGCGTCAT TTTGACTCAC
     7441 GCGGTCGTTA TAGTTCAAAA TCAGTGACAC TTACCGCATT GACAAGCACG CCTCACGGGA
     7501 GCTCCAAGCG GCGACTGAGA TGTCCTAAAT GCACAGCGAC GGATTCGCGC TATTTAGAAA
     7561 GAGAGAGCAA TATTTCAAGA ATGCATGCGT CAATTTTACG CAGACTATCT TTCTAGGGTT
     7621 AATCTAGCTG CATCAGGATC ATATCGTCGG GTCTTTTTTC CGGCTCAGTC ATCGCCCAAG
     7681 CTGGCGCTAT CTGGGCATCG GGGAGGAAGA AGCCCGTGCC TTTTCCCGCG AGGTTGAAGC
     7741 GGCATGGAAA GAGTTTGCCG AGGATGACTG CTGCTGCATT GACGTTGAGC GAAAACGCAC
     7801 GTTTACCATG ATGATTCGGG AAGGTGTGGC CATGCACGCC TTTAACGGTG AACTGTTCGT
     7861 TCAGGCCACC TGGGATACCA GTTCGTCGCG GCTTTTCCGG ACACAGTTCC GGATGGTCAG
     7921 CCCGAAGCGC ATCAGCAACC CGAACAATAC CGGCGACAGC CGGAACTGCC GTGCCGGTGT
     7981 GCAGATTAAT GACAGCGGTG CGGCGCTGGG ATATTACGTC AGCGAGGACG GGTATCCTGG
     8041 CTGGATGCCG CAGAAATGGA CATGGATACC CCGTGAGTTA CCCGGCGGGC GCGCTTGGCG
     8101 TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC
     8161 ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA
     8221 TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT
     8281 TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC
     8341 TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA
     8401 AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA
     8461 AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG
     8521 CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG
     8581 ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT
     8641 CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT
     8701 TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC
     8761 TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT
     8821 GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT
     8881 AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC
     8941 TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA
     9001 GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT
     9061 GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA
     9121 CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT
     9181 CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA
     9241 GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT
     9301 CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA
     9361 CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA G
//