LOCUS       PB_H1H2DBD-HALO         9357 bp ds-DNA     circular     01-NOV-2022
DEFINITION  .
KEYWORDS    "Sequence" "Box" "marker:NeoR/KanR, AmpR"
COMMENT     New Assembly 9357bp ds-DNA circular SYN  30-AUG-2019
FEATURES             Location/Qualifiers
     promoter        complement(3..9)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        complement(10..27)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        complement(28..33)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind    48..69
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     rep_origin      complement(357..944)
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             complement(1115..1906)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             complement(1907..1975)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     promoter        complement(1976..2080)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     rep_origin      complement(2106..2560)
                     /label="f1 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     2701..2717
                     /label="M13 fwd"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
     misc_feature    2847..2859
                     /label="PB Terminal Repeat"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     promoter        3362..3573
                     /label="EF-1α core promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     LTR             3586..3854
                     /label="5' LTR (truncated)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          3795..3814
                     /label="oST085"
                     /note="sequence: gaccctgcttgctcaactct"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          3862..3894
                     /label="oST198"
                     /note="sequence: tgaccggcgcctactatgaagcagagttccaac"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    3877..4242
                     /label="HSF2 DBD"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          4125..4147
                     /label="H1H2DBD.qPCR.fwd"
                     /note="sequence: ggaaagagatggccctgttgaat"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(4222..4252)
                     /label="oST199"
                     /note="sequence: cactgtcctgacgaattttattttcctctgg"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          4235..4260
                     /label="oST200"
                     /note="sequence: aaattcgtcaggacagtgtcacccgg"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    4243..5419
                     /label="HSF1"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     primer          complement(4286..4307)
                     /label="H1H2DBD.qPCR.rev"
                     /note="sequence: tccatacactcctgtttcccct"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          5376..5395
                     /label="H1H2DBD.qPCR.fwd"
                     /note="sequence: gacaggcactgaaccccata"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(5400..5434)
                     /label="oST201"
                     /note="sequence: gactagtcctcgaggaggagacagtggggtccttg"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             5440..5460
                     /label="TEV site"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             5470..6360
                     /label="HaloTag®"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(5507..5527)
                     /label="H1H2DBD.qPCR.rev"
                     /note="sequence: cgcccaggacttccacataat"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             6361..6384
                     /label="FLAG"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    6409..6981
                     /label="IRES"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             6990..7784
                     /label="NeoR/KanR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     polyA_signal    7906..8027
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(8809..8821)
                     /label="PB Terminal Repeat"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     complement(9312..9328)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
     protein_bind    9336..9352
                     /label="lac operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
ORIGIN
        1 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
       61 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
      121 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
      181 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
      241 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      301 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
      361 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
      421 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      481 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
      541 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
      601 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
      661 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
      721 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
      781 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
      841 ggaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
      901 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
      961 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     1021 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     1081 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     1141 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     1201 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     1261 cacgctcacc ggttccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     1321 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     1381 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     1441 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     1501 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     1561 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     1621 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     1681 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     1741 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     1801 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     1861 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     1921 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     1981 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     2041 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     2101 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     2161 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     2221 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     2281 ctccaacgtc aaaggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     2341 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     2401 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     2461 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     2521 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc attcaggctg
     2581 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa
     2641 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggtttcccag tcacgacgtt
     2701 gtaaaacgac ggccagtgag cgcgcctcgt tcattcacgt ttttgaaccc gtggaggacg
     2761 ggcagactcg cggtgcaaat gtgttttaca gcgtgatgga gcagatgaag atgctcgaca
     2821 cgctgcagaa cacgcagcta gattaaccct agaaagataa tcatattgtg acgtacgtta
     2881 aagataatca tgtgtaaaat tgacgcatgt gttttatcgg tctgtatatc gaggtttatt
     2941 tattaatttg aatagatatt aagttttatt atatttacac ttacatacta ataataaatt
     3001 caacaaacaa tttatttatg tttatttatt tattaaaaaa aacaaaaact caaaatttct
     3061 tctataaagt aacaaaactt ttatgaggga cagccccccc ccaaagcccc cagggatgta
     3121 attacgtccc tcccccgcta gggggcagca gcgagccgcc cggggctccg ctccggtccg
     3181 gcgctccccc cgcatccccg agccggcagc gtgcggggac agcccgggca cggggaaggt
     3241 ggcacgggat cgctttcctc tgaacgcttc tcgctgctct ttgagcctgc agacacctgg
     3301 ggggatacgg ggaaaaggcc tccacggcca aggatctgcg atcgctccgg tgcccgtcag
     3361 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
     3421 acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
     3481 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
     3541 ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct
     3601 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
     3661 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
     3721 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
     3781 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
     3841 cgccgttaca gatccaagct gtgaccggcg cctactatga agcagagttc caacgtgccg
     3901 gctttcctca gcaagctgtg gacgcttgtg gaggaaaccc acaccaacga gttcatcacc
     3961 tggagtcaga atggacaaag ttttctggtc ttggatgagc aaagatttgc aaaggaaatt
     4021 cttcctaagt acttcaaaca caataacatg gcgagctttg tgagacaact aaatatgtat
     4081 ggcttccgaa aagtagtgca tatcgaatct ggaattatca aacaggaaag agatggccct
     4141 gttgaatttc agcatcctta tttcaagcaa ggccaggatg acctgttgga gaacattaaa
     4201 aggaaggttt catcttcaaa accagaggaa aataaaattc gtcaggacag tgtcacccgg
     4261 ctgttgacag atgtgcagct gatgaagggg aaacaggagt gtatggactc caagctcctg
     4321 gccatgaagc acgagaacga ggccctgtgg cgggaggtgg ccagccttcg gcagaagcat
     4381 gcccagcagc aaaaagttgt caacaagctc attcagttcc tgatctcact ggtgcagtcg
     4441 aaccggatcc tgggggtgaa gagaaagatc cctctgatgt tgagtgacag caactcagca
     4501 cactctgtgc ccaagtatgg tcgacagtac tccctggagc atgtccatgg tcctggccca
     4561 tactcagctc catctccagc ctacagcagc tctagccttt actcctctga tgctgtcacc
     4621 agctctggac ccataatctc cgatatcact gagctggctc ccaccagccc tttggcctcc
     4681 ccaggcagga gcatagatga gaggcctctg tccagcagca ctctggtccg tgtcaagcaa
     4741 gagcccccca gcccacctca cagccctcgg gtactggagg cgagccctgg gcgcccatcc
     4801 tccatggata cccctttgtc cccaactgcc ttcattgact ccatccttcg agagagcgag
     4861 cctacccctg ctgcctcaaa cacagcccct atggacacaa ccggagccca agcccccgca
     4921 ctcccgaccc cctccacccc tgagaagtgc ctcagcgtag cctgcctaga caagaacgag
     4981 ctaagtgatc acctggatgc catggactcc aacctggaca acctgcagac catgctgaca
     5041 agccacggct tcagtgtgga caccagtgcc ctgctggacc tgttcagccc ctcggtgacc
     5101 atgcccgaca tgagcctgcc tgacctggac agcagcctgg ccagcattca ggagcttctg
     5161 tctccacaag agcctcccag gcctattgag gcagagaaca gtaaccccga ctcaggaaag
     5221 cagctggtgc actacacggc tcagcctctg ttcctgctgg atcctgatgc tgtggacaca
     5281 gggagcagtg agctgcctgt gctctttgag ctgggggaga gctcctactt ctctgagggg
     5341 gatgactaca cggatgatcc caccatctct cttctgacag gcactgaacc ccataaagcc
     5401 aaggacccca ctgtctcctc ctcgaggact agtcctcgag aggatctgta ctttcagagc
     5461 ggaggctcca tggcagaaat cggtactggc tttccattcg acccccatta tgtggaagtc
     5521 ctgggcgagc gcatgcacta cgtcgatgtt ggtccgcgcg atggcacccc tgtgctgttc
     5581 ctgcacggta acccgacctc ctcctacgtg tggcgcaaca tcatcccgca tgttgcaccg
     5641 acccatcgct gcattgctcc agacctgatc ggtatgggca aatccgacaa accagacctg
     5701 ggttatttct tcgacgacca cgtccgcttc atggatgcct tcatcgaagc cctgggtctg
     5761 gaagaggtcg tcctggtcat tcacgactgg ggctccgctc tgggtttcca ctgggccaag
     5821 cgcaatccag agcgcgtcaa aggtattgca tttatggagt tcatccgccc tatcccgacc
     5881 tgggacgaat ggccagaatt tgcccgcgag accttccagg ccttccgcac caccgacgtc
     5941 ggccgcaagc tgatcatcga tcagaacgtt tttatcgagg gtacgctgcc gatgggtgtc
     6001 gtccgcccgc tgactgaagt cgagatggac cattaccgcg agccgttcct gaatcctgtt
     6061 gaccgcgagc cactgtggcg cttcccaaac gagctgccaa tcgccggtga gccagcgaac
     6121 atcgtcgcgc tggtcgaaga atacatggac tggctgcacc agtcccctgt cccgaagctg
     6181 ctgttctggg gcaccccagg cgttctgatc ccaccggccg aagccgctcg cctggccaaa
     6241 agcctgccta actgcaaggc tgtggacatc ggcccgggtc tgaatctgct gcaagaagac
     6301 aacccggacc tgatcggcag cgagatcgcg cgctggctgt cgacgctcga gatttccggc
     6361 gactacaaag acgatgacga caagcgtacg taaggatccg cggccgcgcc cctctccctc
     6421 ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta
     6481 tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc
     6541 tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct
     6601 gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt
     6661 agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa
     6721 gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg
     6781 gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga
     6841 tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac
     6901 atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt
     6961 cctttgaaaa acacgataat acctccggaa tgattgaaca agatggattg cacgcaggtt
     7021 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     7081 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     7141 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
     7201 ccgcgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     7261 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     7321 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     7381 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     7441 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     7501 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     7561 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     7621 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     7681 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     7741 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagtcgac aatcaacctc
     7801 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     7861 tatgtggata cgctgcttta atgcctttgt atcatgcgtt aactaaactt gtttattgca
     7921 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     7981 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggaat
     8041 tgactcaaat gatgtcaatt agtctatcag aagctcatct ggtctccctt ccgggggaca
     8101 agacatccct gtttaatatt taaacagcag tgttcccaaa ctgggttctt atatcccttg
     8161 ctctggtcaa ccaggttgca gggtttcctg tcctcacagg aacgaagtcc ctaaagaaac
     8221 agtggcagcc aggtttagcc ccggaattga ctggattcct tttttagggc ccattggtat
     8281 ggctttttcc ccgtatcccc ccaggtgtct gcaggctcaa agagcagcga gaagcgttca
     8341 gaggaaagcg atcccgtgcc accttccccg tgcccgggct gtccccgcac gctgccggct
     8401 cggggatgcg gggggagcgc cggaccggag cggagccccg ggcggctcgc tgctgccccc
     8461 tagcggggga gggacgtaat tacatccctg ggggctttgg gggggggctg tccctgatat
     8521 ctataacaag aaatatatat ataataagtt atcacgtaag tagaacatga aataacaata
     8581 taattatcgt atgagttaat cttaaagtca cgtaaaagat aatcatgcgt cattttgact
     8641 cacgcggtcg ttatagttca aaatcagtga cacttaccgc attgacaagc acgcctcacg
     8701 ggagctccaa gcggcgactg agatgtccta aatgcacagc gacggattcg cgctatttag
     8761 aaagagagag caatatttca agaatgcatg cgtcaatttt acgcagacta tctttctagg
     8821 gttaatctag ctgcatcagg atcatatcgt cgggtctttt ttccggctca gtcatcgccc
     8881 aagctggcgc tatctgggca tcggggagga agaagcccgt gccttttccc gcgaggttga
     8941 agcggcatgg aaagagtttg ccgaggatga ctgctgctgc attgacgttg agcgaaaacg
     9001 cacgtttacc atgatgattc gggaaggtgt ggccatgcac gcctttaacg gtgaactgtt
     9061 cgttcaggcc acctgggata ccagttcgtc gcggcttttc cggacacagt tccggatggt
     9121 cagcccgaag cgcatcagca acccgaacaa taccggcgac agccggaact gccgtgccgg
     9181 tgtgcagatt aatgacagcg gtgcggcgct gggatattac gtcagcgagg acgggtatcc
     9241 tggctggatg ccgcagaaat ggacatggat accccgtgag ttacccggcg ggcgcgcttg
     9301 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattcca
//