LOCUS       3xSV40NLS-Halo-HSF2     9480 bp ds-DNA     circular     02-SEP-2022
DEFINITION  .
KEYWORDS    "Sequence" "Box" "creator:luye" "marker:AmpR, NeoR/KanR"
FEATURES             Location/Qualifiers
     CDS             complement(682..1473)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          1420..1439
                     /label="oST429"
                     /note="sequence: TCGTGCACCCAACTGATCTT"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             complement(1474..1542)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     promoter        complement(1543..1647)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     rep_origin      complement(1673..2127)
                     /label="f1 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     2268..2284
                     /label="M13 fwd"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
     misc_feature    2911..3434
                     /label="EF1a prom"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     promoter        2929..3140
                     /label="EF-1α core promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     LTR             3153..3421
                     /label="5' LTR (truncated)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(3260..3302)
                     /label="oST780"
                     /note="sequence: GCCCGGTCTCGACCTGAGCTTTAAACTTACCTAGACGGCGGAC"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    3283..3733
                     /label="gBlock"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          3362..3381
                     /label="oST085"
                     /note="sequence: GACCCTGCTTGCTCAACTCT"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    3443..3443
                     /label="EX-MCS"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             3453..3521
                     /label="3xFLAG"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             3453..6089
                     /label="Translation 3453-6089"
                     /translation="MDYKDHDGDYKDHDIDYKDDDDKLPKKKRKVDPKKKRKVDPKKKRKVGSMAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIGSEIARWLSTLEISGEPTTEDLYFQSDNSRMKQSSNVPAFLSKLWTLVEETHTNEFITWSQNGQSFLVLDEQRFAKEILPKYFKHNNMASFVRQLNMYGFRKVVHIESGIIKQERDGPVEFQHPYFKQGQDDLLENIKRKVSSSKPEENKIRQEDLTKIISSAQKVQIKQETIESRLSELKSENESLWKEVSELRAKHAQQQQVIRKIVQFIVTLVQNNQLVSLKRKRPLLLNTNGAPKKNLYQHIVKEPTDNHHHKVPHSRTEGLKSRERISDDIIIYDVTDDNVDEENIPVIPETNEDVVVDSSNQYPDIVIVEDDNEDEYAPVIQSGEQSEPAREPLRVGSAGSSSPLMSSAVQLNGSSSLTSEDPVTMMDSILNDNINLLGKVELLDYLDSIDCSLEDFQAMLSGRQFSIDPDLLVDSENKGLEATKSSVVQHVSEEGRKSKSKPDKQLIQYTAFPLLAFLDGNSASAIEQGSTTASSEVVPSVDKPIEVDELLDSSLDPEPTQSKLVRLEPLTEAEASEATLFYLCELAPAPLDSDMPLLDS*"
     CDS             3525..3545
                     /label="SV40 NLS"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     CDS             3549..3569
                     /label="SV40 NLS"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     CDS             3573..3593
                     /label="SV40 NLS"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     CDS             3600..4490
                     /label="HaloTag"
                     /ApEinfo_revcolor="#333399"
                     /ApEinfo_fwdcolor="#333399"
     primer          3614..3634
                     /label="oST450"
                     /note="sequence: TACTGGCTTTCCATTCGACCC"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          complement(3675..3694)
                     /label="oST099"
                     /note="sequence: CCATCGCGCGGACCAACATC"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          3714..3752
                     /label="oST779"
                     /note="sequence: CACGGTAACCCGACCTCCTCCTACGTGTGGCGCAACATC"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(3882..3906)
                     /label="TN010"
                     /note="sequence: CCAGGACGACCTCTTCCAGACCCAG"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          4144..4170
                     /label="TN009"
                     /note="sequence: CTGAAGTCGAGATGGACCATTACCGCG"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          4381..4400
                     /label="oST183"
                     /note="sequence: ACTGCAAGGCTGTGGACATC"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(4405..4423)
                     /label="oST451"
                     /note="sequence: TGCAGCAGATTCAGACCCG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(4405..4423)
                     /label="oST451"
                     /note="sequence: TGCAGCAGATTCAGACCCG"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          4416..4435
                     /label="Halo-H2.qPCR.fwd"
                     /note="sequence: CTGCTGCAAGAAGACAACCC"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             4503..4523
                     /label="TEV protease site"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     primer          4530..4557
                     /label="Maybe primer 2"
                     /note="sequence: TCTAGAatgaagcagagttccaacgtgc"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     exon            4535..4628
                     /label="exon"
                     /ApEinfo_revcolor="#708090"
                     /ApEinfo_fwdcolor="#708090"
     gene            4535..6089
                     /label="Hsf2"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    4536..4868
                     /label="HSF2DBD"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             4536..6089
                     /label="Hsf2"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     CDS             4536..6089
                     /label="Translation 4536-6089"
                     /translation="MKQSSNVPAFLSKLWTLVEETHTNEFITWSQNGQSFLVLDEQRFAKEILPKYFKHNNMASFVRQLNMYGFRKVVHIESGIIKQERDGPVEFQHPYFKQGQDDLLENIKRKVSSSKPEENKIRQEDLTKIISSAQKVQIKQETIESRLSELKSENESLWKEVSELRAKHAQQQQVIRKIVQFIVTLVQNNQLVSLKRKRPLLLNTNGAPKKNLYQHIVKEPTDNHHHKVPHSRTEGLKSRERISDDIIIYDVTDDNVDEENIPVIPETNEDVVVDSSNQYPDIVIVEDDNEDEYAPVIQSGEQSEPAREPLRVGSAGSSSPLMSSAVQLNGSSSLTSEDPVTMMDSILNDNINLLGKVELLDYLDSIDCSLEDFQAMLSGRQFSIDPDLLVDSENKGLEATKSSVVQHVSEEGRKSKSKPDKQLIQYTAFPLLAFLDGNSASAIEQGSTTASSEVVPSVDKPIEVDELLDSSLDPEPTQSKLVRLEPLTEAEASEATLFYLCELAPAPLDSDMPLLDS*"
     primer          complement(4577..4596)
                     /label="Halo-H2.qPCR.rev"
                     /note="sequence: tttcctccacaagcgtccac"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     exon            4629..4737
                     /label="exon"
                     /ApEinfo_revcolor="#708090"
                     /ApEinfo_fwdcolor="#708090"
     exon            4738..4865
                     /label="exon"
                     /ApEinfo_revcolor="#708090"
                     /ApEinfo_fwdcolor="#708090"
     misc_feature    4857..4901
                     /label="NLS1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     exon            4866..4990
                     /label="exon"
                     /ApEinfo_revcolor="#708090"
                     /ApEinfo_fwdcolor="#708090"
     misc_feature    4890..5111
                     /label="HR-A/B "
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          4902..4927
                     /label="oST204"
                     /note="sequence: caggaagatttaacaaaaattattag"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     exon            4991..5066
                     /label="exon"
                     /ApEinfo_revcolor="#708090"
                     /ApEinfo_fwdcolor="#708090"
     exon            5067..5128
                     /label="exon"
                     /ApEinfo_revcolor="#708090"
                     /ApEinfo_fwdcolor="#708090"
     misc_feature    5118..5162
                     /label="NLS2"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     exon            5129..5216
                     /label="exon"
                     /ApEinfo_revcolor="#708090"
                     /ApEinfo_fwdcolor="#708090"
     exon            5217..5365
                     /label="exon"
                     /ApEinfo_revcolor="#708090"
                     /ApEinfo_fwdcolor="#708090"
     exon            5366..5602
                     /label="exon"
                     /ApEinfo_revcolor="#708090"
                     /ApEinfo_fwdcolor="#708090"
     exon            5603..5708
                     /label="exon"
                     /ApEinfo_revcolor="#708090"
                     /ApEinfo_fwdcolor="#708090"
     exon            5709..5793
                     /label="exon"
                     /ApEinfo_revcolor="#708090"
                     /ApEinfo_fwdcolor="#708090"
     STS             5782..6089
                     /label="Hsf2"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     exon            5794..6089
                     /label="exon"
                     /ApEinfo_revcolor="#708090"
                     /ApEinfo_fwdcolor="#708090"
     STS             5869..5987
                     /label="Hsf2"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    6092..6097
                     /label="EX-MCS"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(6098..6113)
                     /label="oST614"
                     /note="sequence: GGGGAGGGAGAGGGGC"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     RBS             6098..6671
                     /label="IRES"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(6180..6199)
                     /label="oST086"
                     /note="sequence: AAGACGGCAATATGGTGGAA"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(6185..6212)
                     /label="oST438"
                     /note="sequence: CTCACATTGCCAAAAGACGGCAATATGG"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             6680..7474
                     /label="NeoR/KanR"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          6720..6740
                     /label="oST449"
                     /note="sequence: CTTGGGTGGAGAGGCTATTCG"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          6930..6949
                     /label="oST425"
                     /note="sequence: TCACTGAAGCGGGAAGGGAC"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(7453..7492)
                     /label="oST660"
                     /note="sequence: CAGAGGTTGATTGTCGACTCAGAAGAACTCGTCAAGAAGG"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          7475..7500
                     /label="oST661 copy"
                     /note="sequence: GTCGACAATCAACCTCTGGATTACAA"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     polyA_signal    7596..7717
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     complement(9002..9018)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
     protein_bind    9026..9042
                     /label="lac operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     promoter        complement(9050..9056)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        complement(9057..9074)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        complement(9075..9080)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind    9095..9116
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     rep_origin      complement(9404..511)
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC
       61 TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA
      121 GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC
      181 ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA
      241 ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG
      301 CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA
      361 GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAGAGTTGG
      421 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA
      481 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC
      541 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG
      601 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA
      661 TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT
      721 CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG
      781 GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGT
      841 TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC
      901 AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC
      961 GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC
     1021 GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC
     1081 CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA
     1141 GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT
     1201 GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA
     1261 GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA
     1321 TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG
     1381 GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC
     1441 AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC
     1501 AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA
     1561 TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA
     1621 GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTAAATTGTA
     1681 AGCGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT AAATCAGCTC ATTTTTTAAC
     1741 CAATAGGCCG AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA GATAGGGTTG
     1801 AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC CAACGTCAAA
     1861 GGCGAAAAAC CGTCTATCAG GGCGATGGCC CACTACGTGA ACCATCACCC TAATCAAGTT
     1921 TTTTGGGGTC GAGGTGCCGT AAAGCACTAA ATCGGAACCC TAAAGGGAGC CCCCGATTTA
     1981 GAGCTTGACG GGGAAAGCCG GCGAACGTGG CGAGAAAGGA AGGGAAGAAA GCGAAAGGAG
     2041 CGGGCGCTAG GGCGCTGGCA AGTGTAGCGG TCACGCTGCG CGTAACCACC ACACCCGCCG
     2101 CGCTTAATGC GCCGCTACAG GGCGCGTCCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG
     2161 AAGGGCGATC GGTGCGGGCC TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG
     2221 CAAGGCGATT AAGTTGGGTA ACGCCAGGGT TTCCCAGTCA CGACGTTGTA AAACGACGGC
     2281 CAGTGAGCGC GCCTCGTTCA TTCACGTTTT TGAACCCGTG GAGGACGGGC AGACTCGCGG
     2341 TGCAAATGTG TTTTACAGCG TGATGGAGCA GATGAAGATG CTCGACACGC TGCAGAACAC
     2401 GCAGCTAGAT TAACCCTAGA AAGATAATCA TATTGTGACG TACGTTAAAG ATAATCATGT
     2461 GTAAAATTGA CGCATGTGTT TTATCGGTCT GTATATCGAG GTTTATTTAT TAATTTGAAT
     2521 AGATATTAAG TTTTATTATA TTTACACTTA CATACTAATA ATAAATTCAA CAAACAATTT
     2581 ATTTATGTTT ATTTATTTAT TAAAAAAAAC AAAAACTCAA AATTTCTTCT ATAAAGTAAC
     2641 AAAACTTTTA TGAGGGACAG CCCCCCCCCA AAGCCCCCAG GGATGTAATT ACGTCCCTCC
     2701 CCCGCTAGGG GGCAGCAGCG AGCCGCCCGG GGCTCCGCTC CGGTCCGGCG CTCCCCCCGC
     2761 ATCCCCGAGC CGGCAGCGTG CGGGGACAGC CCGGGCACGG GGAAGGTGGC ACGGGATCGC
     2821 TTTCCTCTGA ACGCTTCTCG CTGCTCTTTG AGCCTGCAGA CACCTGGGGG GATACGGGGA
     2881 AAAGGCCTCC ACGGCCAAGG ATCTGCGATC GCTCCGGTGC CCGTCAGTGG GCAGAGCGCA
     2941 CATCGCCCAC AGTCCCCGAG AAGTTGGGGG GAGGGGTCGG CAATTGAACG GGTGCCTAGA
     3001 GAAGGTGGCG CGGGGTAAAC TGGGAAAGTG ATGTCGTGTA CTGGCTCCGC CTTTTTCCCG
     3061 AGGGTGGGGG AGAACCGTAT ATAAGTGCAG TAGTCGCCGT GAACGTTCTT TTTCGCAACG
     3121 GGTTTGCCGC CAGAACACAG CTGAAGCTTC GAGGGGCTCG CATCTCTCCT TCACGCGCCC
     3181 GCCGCCCTAC CTGAGGCCGC CATCCACGCC GGTTGAGTCG CGTTCTGCCG CCTCCCGCCT
     3241 GTGGTGCCTC CTGAACTGCG TCCGCCGTCT AGGTAAGTTT AAAGCTCAGG TCGAGACCGG
     3301 GCCTTTGTCC GGCGCTCCCT TGGAGCCTAC CTAGACTCAG CCGGCTCTCC ACGCTTTGCC
     3361 TGACCCTGCT TGCTCAACTC TACGTCTTTG TTTCGTTTTC TGTTCTGCGC CGTTACAGAT
     3421 CCAAGCTGTG ACCGGCGCCT ACTCTAGCCA CCATGGACTA CAAAGACCAT GACGGTGATT
     3481 ATAAAGATCA TGACATCGAT TACAAGGATG ACGATGACAA GCTTCCAAAA AAGAAGAGAA
     3541 AGGTAGATCC cAAgAAaAAG AGAAAGGTcG ATCCAAAgAA GAAaAGAAAa GTgGGCTCCA
     3601 TGGCAGAAAT CGGTACTGGC TTTCCATTCG ACCCCCATTA TGTGGAAGTC CTGGGCGAGC
     3661 GCATGCACTA CGTCGATGTT GGTCCGCGCG ATGGCACCCC TGTGCTGTTC CTGCACGGTA
     3721 ACCCGACCTC CTCCTACGTG TGGCGCAACA TCATCCCGCA TGTTGCACCG ACCCATCGCT
     3781 GCATTGCTCC AGACCTGATC GGTATGGGCA AATCCGACAA ACCAGACCTG GGTTATTTCT
     3841 TCGACGACCA CGTCCGCTTC ATGGATGCCT TCATCGAAGC CCTGGGTCTG GAAGAGGTCG
     3901 TCCTGGTCAT TCACGACTGG GGCTCCGCTC TGGGTTTCCA CTGGGCCAAG CGCAATCCAG
     3961 AGCGCGTCAA AGGTATTGCA TTTATGGAGT TCATCCGCCC TATCCCGACC TGGGACGAAT
     4021 GGCCAGAATT TGCCCGCGAG ACCTTCCAGG CCTTCCGCAC CACCGACGTC GGCCGCAAGC
     4081 TGATCATCGA TCAGAACGTT TTTATCGAGG GTACGCTGCC GATGGGTGTC GTCCGCCCGC
     4141 TGACTGAAGT CGAGATGGAC CATTACCGCG AGCCGTTCCT GAATCCTGTT GACCGCGAGC
     4201 CACTGTGGCG CTTCCCAAAC GAGCTGCCAA TCGCCGGTGA GCCAGCGAAC ATCGTCGCGC
     4261 TGGTCGAAGA ATACATGGAC TGGCTGCACC AGTCCCCTGT CCCGAAGCTG CTGTTCTGGG
     4321 GCACCCCAGG CGTTCTGATC CCACCGGCCG AAGCCGCTCG CCTGGCCAAA AGCCTGCCTA
     4381 ACTGCAAGGC TGTGGACATC GGCCCGGGTC TGAATCTGCT GCAAGAAGAC AACCCGGACC
     4441 TGATCGGCAG CGAGATCGCG CGCTGGCTGT CGACGCTCGA GATTTCCGGC GAGCCAACCA
     4501 CTGAGGATCT GTACTTTCAG AGCGATAACT CTAGAatgaa gcagagttcc aacgtgccgg
     4561 ctttcctcag caagctgtgg acgcttgtgg aggaaaccca caccaacgag ttcatcacct
     4621 ggagtcagaa tggacaaagt tttctggtct tggatgagca aagatttgca aaggaaattc
     4681 ttcctaagta cttcaaacac aataacatgg cgagctttgt gagacaacta aatatgtatg
     4741 gcttccgaaa agtagtgcat atcgaatctg gaattatcaa acaggaaaga gatggccctg
     4801 ttgaatttca gcatccttat ttcaagcaag gccaggatga cctgttggag aacattaaaa
     4861 ggaaggtttc atcttcaaaa ccagaggaaa ataaaattcg tcaggaagat ttaacaaaaa
     4921 ttattagtag tgctcagaag gttcaaataa aacaagaaac tattgagtcc aggctttcag
     4981 aattaaaaag tgagaatgaa tccctttgga aggaggtgtc agaactaaga gcaaagcatg
     5041 cccagcagca acaagttatt cggaagattg tccagtttat tgttacattg gttcagaata
     5101 atcaacttgt gagtttaaaa cgtaaaaggc ctctacttct aaacacaaat ggagccccaa
     5161 agaagaatct atatcagcac atagtcaaag aaccaactga taatcaccat cataaagttc
     5221 cacacagcag gactgaaggt ttaaagtcaa gagaacggat ttcagatgac ataattattt
     5281 atgatgttac tgacgataat gtggatgaag aaaatattcc agttattcca gaaacaaatg
     5341 aggatgttgt agtggattcc tccaaccagt atcctgacat tgtcattgtt gaagatgaca
     5401 acgaggatga gtatgctcct gtcattcaga gtggagagca gagtgaacca gccagagaac
     5461 ccttacgtgt ggggagtgct ggcagcagca gccctctcat gtctagtgct gtccagctaa
     5521 acggctcctc cagtctgacc tcagaagacc ctgtgaccat gatggactcc attctgaatg
     5581 acaacattaa cctgttagga aaggttgagc tgttggatta ccttgacagt attgattgca
     5641 gtttagagga cttccaagct atgctctcag gaagacagtt tagcatagac ccagatcttc
     5701 tggttgattc tgagaataag ggactagaag ctaccaagag cagtgttgtt caacatgtgt
     5761 cagaagaggg aagaaaatct aaatccaagc cagacaaaca acttatccag tatactgcct
     5821 ttccacttct tgcattcctg gatgggaact ctgcatctgc tattgaacag gggagtacaa
     5881 ctgcatcgtc agaagttgtg ccttctgtag ataaacccat agaagtcgat gagctcctgg
     5941 atagcagcct ggatccagaa ccgacccaga gtaagcttgt ccgcctggaa ccattgactg
     6001 aagcggaagc tagtgaagcc acactcttct atttatgtga acttgctcct gcacctctgg
     6061 atagtgatat gccgctttta gatagttaaG CGGCCGCGCC CCTCTCCCTC CCCCCCCCCT
     6121 AACGTTACTG GCCGAAGCCG CTTGGAATAA GGCCGGTGTG CGTTTGTCTA TATGTTATTT
     6181 TCCACCATAT TGCCGTCTTT TGGCAATGTG AGGGCCCGGA AACCTGGCCC TGTCTTCTTG
     6241 ACGAGCATTC CTAGGGGTCT TTCCCCTCTC GCCAAAGGAA TGCAAGGTCT GTTGAATGTC
     6301 GTGAAGGAAG CAGTTCCTCT GGAAGCTTCT TGAAGACAAA CAACGTCTGT AGCGACCCTT
     6361 TGCAGGCAGC GGAACCCCCC ACCTGGCGAC AGGTGCCTCT GCGGCCAAAA GCCACGTGTA
     6421 TAAGATACAC CTGCAAAGGC GGCACAACCC CAGTGCCACG TTGTGAGTTG GATAGTTGTG
     6481 GAAAGAGTCA AATGGCTCTC CTCAAGCGTA TTCAACAAGG GGCTGAAGGA TGCCCAGAAG
     6541 GTACCCCATT GTATGGGATC TGATCTGGGG CCTCGGTGCA CATGCTTTAC ATGTGTTTAG
     6601 TCGAGGTTAA AAAAACGTCT AGGCCCCCCG AACCACGGGG ACGTGGTTTT CCTTTGAAAA
     6661 ACACGATAAT ACCTCCGGAA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC
     6721 TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC
     6781 CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC
     6841 CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCGCGACGGG
     6901 CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT
     6961 GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC
     7021 CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA
     7081 CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA
     7141 TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT
     7201 CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC
     7261 GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT
     7321 GGCGGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG
     7381 CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT
     7441 CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGTCGAC AATCAACCTC TGGATTACAA
     7501 AATTTGTGAA AGATTGACTG GTATTCTTAA CTATGTTGCT CCTTTTACGC TATGTGGATA
     7561 CGCTGCTTTA ATGCCTTTGT ATCATGCGTT AACTAAACTT GTTTATTGCA GCTTATAATG
     7621 GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT
     7681 CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGGAAT TGACTCAAAT
     7741 GATGTCAATT AGTCTATCAG AAGCTCATCT GGTCTCCCTT CCGGGGGACA AGACATCCCT
     7801 GTTTAATATT TAAACAGCAG TGTTCCCAAA CTGGGTTCTT ATATCCCTTG CTCTGGTCAA
     7861 CCAGGTTGCA GGGTTTCCTG TCCTCACAGG AACGAAGTCC CTAAAGAAAC AGTGGCAGCC
     7921 AGGTTTAGCC CCGGAATTGA CTGGATTCCT TTTTTAGGGC CCATTGGTAT GGCTTTTTCC
     7981 CCGTATCCCC CCAGGTGTCT GCAGGCTCAA AGAGCAGCGA GAAGCGTTCA GAGGAAAGCG
     8041 ATCCCGTGCC ACCTTCCCCG TGCCCGGGCT GTCCCCGCAC GCTGCCGGCT CGGGGATGCG
     8101 GGGGGAGCGC CGGACCGGAG CGGAGCCCCG GGCGGCTCGC TGCTGCCCCC TAGCGGGGGA
     8161 GGGACGTAAT TACATCCCTG GGGGCTTTGG GGGGGGGCTG TCCCTGATAT CTATAACAAG
     8221 AAATATATAT ATAATAAGTT ATCACGTAAG TAGAACATGA AATAACAATA TAATTATCGT
     8281 ATGAGTTAAT CTTAAAGTCA CGTAAAAGAT AATCATGCGT CATTTTGACT CACGCGGTCG
     8341 TTATAGTTCA AAATCAGTGA CACTTACCGC ATTGACAAGC ACGCCTCACG GGAGCTCCAA
     8401 GCGGCGACTG AGATGTCCTA AATGCACAGC GACGGATTCG CGCTATTTAG AAAGAGAGAG
     8461 CAATATTTCA AGAATGCATG CGTCAATTTT ACGCAGACTA TCTTTCTAGG GTTAATCTAG
     8521 CTGCATCAGG ATCATATCGT CGGGTCTTTT TTCCGGCTCA GTCATCGCCC AAGCTGGCGC
     8581 TATCTGGGCA TCGGGGAGGA AGAAGCCCGT GCCTTTTCCC GCGAGGTTGA AGCGGCATGG
     8641 AAAGAGTTTG CCGAGGATGA CTGCTGCTGC ATTGACGTTG AGCGAAAACG CACGTTTACC
     8701 ATGATGATTC GGGAAGGTGT GGCCATGCAC GCCTTTAACG GTGAACTGTT CGTTCAGGCC
     8761 ACCTGGGATA CCAGTTCGTC GCGGCTTTTC CGGACACAGT TCCGGATGGT CAGCCCGAAG
     8821 CGCATCAGCA ACCCGAACAA TACCGGCGAC AGCCGGAACT GCCGTGCCGG TGTGCAGATT
     8881 AATGACAGCG GTGCGGCGCT GGGATATTAC GTCAGCGAGG ACGGGTATCC TGGCTGGATG
     8941 CCGCAGAAAT GGACATGGAT ACCCCGTGAG TTACCCGGCG GGCGCGCTTG GCGTAATCAT
     9001 GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG
     9061 CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG
     9121 CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA
     9181 TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA
     9241 CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG
     9301 TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC
     9361 AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC
     9421 CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC
//