LOCUS       PB-HSF2_DBD_HR-AB       8497 bp ds-DNA     circular     13-DEC-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS             complement(267..335)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     promoter        complement(336..440)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     rep_origin      complement(466..920)
                     /label="f1 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     1061..1077
                     /label="M13 fwd"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
     primer          1178..1197
                     /label="PB Backbone seq4 fwd"
                     /note="sequence: acacgctgcagaacacgcag"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1704..2227
                     /label="EF1a prom"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     promoter        1722..1933
                     /label="EF-1α core promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     LTR             1946..2214
                     /label="5' LTR (truncated)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          2155..2174
                     /label="oST085"
                     /note="sequence: gaccctgcttgctcaactct"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2236..2236
                     /label="EX-MCS"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             2246..2314
                     /label="3xFLAG"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             2324..3214
                     /label="HaloTag"
                     /ApEinfo_revcolor="#333399"
                     /ApEinfo_fwdcolor="#333399"
     primer          2868..2894
                     /label="TN009"
                     /note="sequence: CTGAAGTCGAGATGGACCATTACCGCG"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          3105..3124
                     /label="oST183"
                     /note="sequence: ACTGCAAGGCTGTGGACATC"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             3227..3247
                     /label="TEV protease site"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     exon            3259..3352
                     /label="exon"
                     /ApEinfo_revcolor="#708090"
                     /ApEinfo_fwdcolor="#708090"
     gene            3259..3886
                     /label="Hsf2"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    3260..3592
                     /label="HSF2DBD"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             3260..3886
                     /label="Hsf2"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     exon            3353..3461
                     /label="exon"
                     /ApEinfo_revcolor="#708090"
                     /ApEinfo_fwdcolor="#708090"
     exon            3462..3589
                     /label="exon"
                     /ApEinfo_revcolor="#708090"
                     /ApEinfo_fwdcolor="#708090"
     misc_feature    3581..3625
                     /label="NLS1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     exon            3590..3714
                     /label="exon"
                     /ApEinfo_revcolor="#708090"
                     /ApEinfo_fwdcolor="#708090"
     misc_feature    3614..3835
                     /label="HR-A/B "
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     exon            3715..3790
                     /label="exon"
                     /ApEinfo_revcolor="#708090"
                     /ApEinfo_fwdcolor="#708090"
     exon            3791..3852
                     /label="exon"
                     /ApEinfo_revcolor="#708090"
                     /ApEinfo_fwdcolor="#708090"
     misc_feature    3842..3886
                     /label="NLS2"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     exon            3853..3886
                     /label="exon"
                     /ApEinfo_revcolor="#708090"
                     /ApEinfo_fwdcolor="#708090"
     primer          complement(3867..3886)
                     /label="PB Halo Hsf2 IRES Neo REV (1)"
                     /note="sequence: GGGGGGGGAGGGAGAGGGGCttactttggggctccatttgtgt"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          3867..3909
                     /label="oST704"
                     /note="sequence: acacaaatggagccccaaagtaaGCCCCTCTCCCTCCCCCCCC"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(3867..3909)
                     /label="oST703"
                     /note="sequence: GGGGGGGGAGGGAGAGGGGCttactttggggctccatttgtgt"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(3890..3905)
                     /label="oST614"
                     /note="sequence: GGGGAGGGAGAGGGGC"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          3890..3909
                     /label="PB Halo Hsf2 IRES Neo FWD (2)"
                     /note="sequence: acacaaatggagccccaaagtaaGCCCCTCTCCCTCCCCCCCC"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     RBS             3890..4463
                     /label="IRES"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(3972..3991)
                     /label="oST086"
                     /note="sequence: AAGACGGCAATATGGTGGAA"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             4472..5266
                     /label="NeoR/KanR"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(5254..5284)
                     /label="PB Halo Hsf2 IRES Neo REV (2)"
                     /note="sequence: TCCAGAGGTTGATTGTCGACCAGAGGTTGATTGTCGACTCAGAAGAACTCG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(5254..5304)
                     /label="oST726"
                     /note="sequence: TCCAGAGGTTGATTGTCGACCAGAGGTTGATTGTCGACTCAGAAGAACTCG"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          5265..5304
                     /label="oST710"
                     /note="sequence: GAGTCGACAATCAACCTCTGGTCGACAATCAACCTCTGGA"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          5285..5304
                     /label="PB Halo Hsf2 IRES Neo FWD (1)"
                     /note="sequence: GAGTCGACAATCAACCTCTGGTCGACAATCAACCTCTGGA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     polyA_signal    5406..5527
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     complement(6812..6828)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
     protein_bind    6836..6852
                     /label="lac operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     promoter        complement(6860..6866)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        complement(6867..6884)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        complement(6885..6890)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind    6905..6926
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     rep_origin      complement(7214..7801)
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             complement(7972..266)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
ORIGIN
        1 GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG
       61 CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA
      121 ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA
      181 CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT
      241 TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG
      301 GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA
      361 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT
      421 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTAAATT GTAAGCGTTA
      481 ATATTTTGTT AAAATTCGCG TTAAATTTTT GTTAAATCAG CTCATTTTTT AACCAATAGG
      541 CCGAAATCGG CAAAATCCCT TATAAATCAA AAGAATAGAC CGAGATAGGG TTGAGTGTTG
      601 TTCCAGTTTG GAACAAGAGT CCACTATTAA AGAACGTGGA CTCCAACGTC AAAGGCGAAA
      661 AACCGTCTAT CAGGGCGATG GCCCACTACG TGAACCATCA CCCTAATCAA GTTTTTTGGG
      721 GTCGAGGTGC CGTAAAGCAC TAAATCGGAA CCCTAAAGGG AGCCCCCGAT TTAGAGCTTG
      781 ACGGGGAAAG CCGGCGAACG TGGCGAGAAA GGAAGGGAAG AAAGCGAAAG GAGCGGGCGC
      841 TAGGGCGCTG GCAAGTGTAG CGGTCACGCT GCGCGTAACC ACCACACCCG CCGCGCTTAA
      901 TGCGCCGCTA CAGGGCGCGT CCCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG
      961 ATCGGTGCGG GCCTCTTCGC TATTACGCCA GCTGGCGAAA GGGGGATGTG CTGCAAGGCG
     1021 ATTAAGTTGG GTAACGCCAG GGTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTGAG
     1081 CGCGCCTCGT TCATTCACGT TTTTGAACCC GTGGAGGACG GGCAGACTCG CGGTGCAAAT
     1141 GTGTTTTACA GCGTGATGGA GCAGATGAAG ATGCTCGACA CGCTGCAGAA CACGCAGCTA
     1201 GATTAACCCT AGAAAGATAA TCATATTGTG ACGTACGTTA AAGATAATCA TGTGTAAAAT
     1261 TGACGCATGT GTTTTATCGG TCTGTATATC GAGGTTTATT TATTAATTTG AATAGATATT
     1321 AAGTTTTATT ATATTTACAC TTACATACTA ATAATAAATT CAACAAACAA TTTATTTATG
     1381 TTTATTTATT TATTAAAAAA AACAAAAACT CAAAATTTCT TCTATAAAGT AACAAAACTT
     1441 TTATGAGGGA CAGCCCCCCC CCAAAGCCCC CAGGGATGTA ATTACGTCCC TCCCCCGCTA
     1501 GGGGGCAGCA GCGAGCCGCC CGGGGCTCCG CTCCGGTCCG GCGCTCCCCC CGCATCCCCG
     1561 AGCCGGCAGC GTGCGGGGAC AGCCCGGGCA CGGGGAAGGT GGCACGGGAT CGCTTTCCTC
     1621 TGAACGCTTC TCGCTGCTCT TTGAGCCTGC AGACACCTGG GGGGATACGG GGAAAAGGCC
     1681 TCCACGGCCA AGGATCTGCG ATCGCTCCGG TGCCCGTCAG TGGGCAGAGC GCACATCGCC
     1741 CACAGTCCCC GAGAAGTTGG GGGGAGGGGT CGGCAATTGA ACGGGTGCCT AGAGAAGGTG
     1801 GCGCGGGGTA AACTGGGAAA GTGATGTCGT GTACTGGCTC CGCCTTTTTC CCGAGGGTGG
     1861 GGGAGAACCG TATATAAGTG CAGTAGTCGC CGTGAACGTT CTTTTTCGCA ACGGGTTTGC
     1921 CGCCAGAACA CAGCTGAAGC TTCGAGGGGC TCGCATCTCT CCTTCACGCG CCCGCCGCCC
     1981 TACCTGAGGC CGCCATCCAC GCCGGTTGAG TCGCGTTCTG CCGCCTCCCG CCTGTGGTGC
     2041 CTCCTGAACT GCGTCCGCCG TCTAGGTAAG TTTAAAGCTC AGGTCGAGAC CGGGCCTTTG
     2101 TCCGGCGCTC CCTTGGAGCC TACCTAGACT CAGCCGGCTC TCCACGCTTT GCCTGACCCT
     2161 GCTTGCTCAA CTCTACGTCT TTGTTTCGTT TTCTGTTCTG CGCCGTTACA GATCCAAGCT
     2221 GTGACCGGCG CCTACTCTAG CCACCATGGA CTACAAAGAC CATGACGGTG ATTATAAAGA
     2281 TCATGACATC GATTACAAGG ATGACGATGA CAAGCTTGGC TCCATGGCAG AAATCGGTAC
     2341 TGGCTTTCCA TTCGACCCCC ATTATGTGGA AGTCCTGGGC GAGCGCATGC ACTACGTCGA
     2401 TGTTGGTCCG CGCGATGGCA CCCCTGTGCT GTTCCTGCAC GGTAACCCGA CCTCCTCCTA
     2461 CGTGTGGCGC AACATCATCC CGCATGTTGC ACCGACCCAT CGCTGCATTG CTCCAGACCT
     2521 GATCGGTATG GGCAAATCCG ACAAACCAGA CCTGGGTTAT TTCTTCGACG ACCACGTCCG
     2581 CTTCATGGAT GCCTTCATCG AAGCCCTGGG TCTGGAAGAG GTCGTCCTGG TCATTCACGA
     2641 CTGGGGCTCC GCTCTGGGTT TCCACTGGGC CAAGCGCAAT CCAGAGCGCG TCAAAGGTAT
     2701 TGCATTTATG GAGTTCATCC GCCCTATCCC GACCTGGGAC GAATGGCCAG AATTTGCCCG
     2761 CGAGACCTTC CAGGCCTTCC GCACCACCGA CGTCGGCCGC AAGCTGATCA TCGATCAGAA
     2821 CGTTTTTATC GAGGGTACGC TGCCGATGGG TGTCGTCCGC CCGCTGACTG AAGTCGAGAT
     2881 GGACCATTAC CGCGAGCCGT TCCTGAATCC TGTTGACCGC GAGCCACTGT GGCGCTTCCC
     2941 AAACGAGCTG CCAATCGCCG GTGAGCCAGC GAACATCGTC GCGCTGGTCG AAGAATACAT
     3001 GGACTGGCTG CACCAGTCCC CTGTCCCGAA GCTGCTGTTC TGGGGCACCC CAGGCGTTCT
     3061 GATCCCACCG GCCGAAGCCG CTCGCCTGGC CAAAAGCCTG CCTAACTGCA AGGCTGTGGA
     3121 CATCGGCCCG GGTCTGAATC TGCTGCAAGA AGACAACCCG GACCTGATCG GCAGCGAGAT
     3181 CGCGCGCTGG CTGTCGACGC TCGAGATTTC CGGCGAGCCA ACCACTGAGG ATCTGTACTT
     3241 TCAGAGCGAT AACTCTAGAa tgaagcagag ttccaacgtg ccggctttcc tcagcaagct
     3301 gtggacgctt gtggaggaaa cccacaccaa cgagttcatc acctggagtc agaatggaca
     3361 aagttttctg gtcttggatg agcaaagatt tgcaaaggaa attcttccta agtacttcaa
     3421 acacaataac atggcgagct ttgtgagaca actaaatatg tatggcttcc gaaaagtagt
     3481 gcatatcgaa tctggaatta tcaaacagga aagagatggc cctgttgaat ttcagcatcc
     3541 ttatttcaag caaggccagg atgacctgtt ggagaacatt aaaaggaagg tttcatcttc
     3601 aaaaccagag gaaaataaaa ttcgtcagga agatttaaca aaaattatta gtagtgctca
     3661 gaaggttcaa ataaaacaag aaactattga gtccaggctt tcagaattaa aaagtgagaa
     3721 tgaatccctt tggaaggagg tgtcagaact aagagcaaag catgcccagc agcaacaagt
     3781 tattcggaag attgtccagt ttattgttac attggttcag aataatcaac ttgtgagttt
     3841 aaaacgtaaa aggcctctac ttctaaacac aaatggagcc ccaaagtaaG CCCCTCTCCC
     3901 TCCCCCCCCC CTAACGTTAC TGGCCGAAGC CGCTTGGAAT AAGGCCGGTG TGCGTTTGTC
     3961 TATATGTTAT TTTCCACCAT ATTGCCGTCT TTTGGCAATG TGAGGGCCCG GAAACCTGGC
     4021 CCTGTCTTCT TGACGAGCAT TCCTAGGGGT CTTTCCCCTC TCGCCAAAGG AATGCAAGGT
     4081 CTGTTGAATG TCGTGAAGGA AGCAGTTCCT CTGGAAGCTT CTTGAAGACA AACAACGTCT
     4141 GTAGCGACCC TTTGCAGGCA GCGGAACCCC CCACCTGGCG ACAGGTGCCT CTGCGGCCAA
     4201 AAGCCACGTG TATAAGATAC ACCTGCAAAG GCGGCACAAC CCCAGTGCCA CGTTGTGAGT
     4261 TGGATAGTTG TGGAAAGAGT CAAATGGCTC TCCTCAAGCG TATTCAACAA GGGGCTGAAG
     4321 GATGCCCAGA AGGTACCCCA TTGTATGGGA TCTGATCTGG GGCCTCGGTG CACATGCTTT
     4381 ACATGTGTTT AGTCGAGGTT AAAAAAACGT CTAGGCCCCC CGAACCACGG GGACGTGGTT
     4441 TTCCTTTGAA AAACACGATA ATACCTCCGG AATGATTGAA CAAGATGGAT TGCACGCAGG
     4501 TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG
     4561 CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA
     4621 GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT
     4681 GGCCGCGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA
     4741 CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC
     4801 CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC
     4861 CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC
     4921 CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT
     4981 GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA
     5041 TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG
     5101 CCGGCTGGGT GTGGCGGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA
     5161 AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA
     5221 TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGTCG ACAATCAACC
     5281 TCTGGTCGAC AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG GTATTCTTAA
     5341 CTATGTTGCT CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT ATCATGCGTT
     5401 AACTAAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT
     5461 TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG
     5521 TATCTTATCA TGTCTGGAAT TGACTCAAAT GATGTCAATT AGTCTATCAG AAGCTCATCT
     5581 GGTCTCCCTT CCGGGGGACA AGACATCCCT GTTTAATATT TAAACAGCAG TGTTCCCAAA
     5641 CTGGGTTCTT ATATCCCTTG CTCTGGTCAA CCAGGTTGCA GGGTTTCCTG TCCTCACAGG
     5701 AACGAAGTCC CTAAAGAAAC AGTGGCAGCC AGGTTTAGCC CCGGAATTGA CTGGATTCCT
     5761 TTTTTAGGGC CCATTGGTAT GGCTTTTTCC CCGTATCCCC CCAGGTGTCT GCAGGCTCAA
     5821 AGAGCAGCGA GAAGCGTTCA GAGGAAAGCG ATCCCGTGCC ACCTTCCCCG TGCCCGGGCT
     5881 GTCCCCGCAC GCTGCCGGCT CGGGGATGCG GGGGGAGCGC CGGACCGGAG CGGAGCCCCG
     5941 GGCGGCTCGC TGCTGCCCCC TAGCGGGGGA GGGACGTAAT TACATCCCTG GGGGCTTTGG
     6001 GGGGGGGCTG TCCCTGATAT CTATAACAAG AAATATATAT ATAATAAGTT ATCACGTAAG
     6061 TAGAACATGA AATAACAATA TAATTATCGT ATGAGTTAAT CTTAAAGTCA CGTAAAAGAT
     6121 AATCATGCGT CATTTTGACT CACGCGGTCG TTATAGTTCA AAATCAGTGA CACTTACCGC
     6181 ATTGACAAGC ACGCCTCACG GGAGCTCCAA GCGGCGACTG AGATGTCCTA AATGCACAGC
     6241 GACGGATTCG CGCTATTTAG AAAGAGAGAG CAATATTTCA AGAATGCATG CGTCAATTTT
     6301 ACGCAGACTA TCTTTCTAGG GTTAATCTAG CTGCATCAGG ATCATATCGT CGGGTCTTTT
     6361 TTCCGGCTCA GTCATCGCCC AAGCTGGCGC TATCTGGGCA TCGGGGAGGA AGAAGCCCGT
     6421 GCCTTTTCCC GCGAGGTTGA AGCGGCATGG AAAGAGTTTG CCGAGGATGA CTGCTGCTGC
     6481 ATTGACGTTG AGCGAAAACG CACGTTTACC ATGATGATTC GGGAAGGTGT GGCCATGCAC
     6541 GCCTTTAACG GTGAACTGTT CGTTCAGGCC ACCTGGGATA CCAGTTCGTC GCGGCTTTTC
     6601 CGGACACAGT TCCGGATGGT CAGCCCGAAG CGCATCAGCA ACCCGAACAA TACCGGCGAC
     6661 AGCCGGAACT GCCGTGCCGG TGTGCAGATT AATGACAGCG GTGCGGCGCT GGGATATTAC
     6721 GTCAGCGAGG ACGGGTATCC TGGCTGGATG CCGCAGAAAT GGACATGGAT ACCCCGTGAG
     6781 TTACCCGGCG GGCGCGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT
     6841 ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG
     6901 CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG
     6961 GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC
     7021 GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC
     7081 GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA
     7141 ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG
     7201 CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT
     7261 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA
     7321 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC
     7381 TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT
     7441 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG
     7501 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG
     7561 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT
     7621 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC
     7681 TGAAGCCAGT TACCTTCGGA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC
     7741 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA
     7801 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
     7861 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
     7921 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
     7981 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     8041 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     8101 AATGATACCG CGAGACCCAC GCTCACCGGT TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     8161 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     8221 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     8281 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     8341 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     8401 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     8461 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCC
//