LOCUS       HA-Halo-HSF2            9432 bp ds-DNA     circular     07-FEB-2023
DEFINITION  .
KEYWORDS    "Sequence" "Box" "creator:luye" "marker:AmpR, NeoR/KanR"
FEATURES             Location/Qualifiers
     CDS             complement(650..718)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     promoter        complement(719..823)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     rep_origin      complement(849..1303)
                     /label="f1 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     1444..1460
                     /label="M13 fwd"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
     misc_feature    1590..1602
                     /label="PB Terminal repeat"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1637..1652
                     /label="IR (inverted repeat) truncated?"
                     /ApEinfo_revcolor="#339966"
                     /ApEinfo_fwdcolor="#339966"
     misc_feature    2087..2610
                     /label="EF1a prom"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     promoter        2105..2316
                     /label="EF-1α core promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     LTR             2329..2597
                     /label="5' LTR (truncated)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          2538..2557
                     /label="oST085"
                     /note="sequence: GACCCTGCTTGCTCAACTCT"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    2619..2619
                     /label="EX-MCS"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    2620..2649
                     /label="HA tag"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     CDS             2620..5217
                     /label="FINAL"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /translation="MYPYDVPDYAMDYKDHDGDYKDHDIDYKDDDDKLGSMAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIGSEIARWLSTLEISGEPTTEDLYFQSDNSRMKQSSNVPAFLSKLWTLVEETHTNEFITWSQNGQSFLVLDEQRFAKEILPKYFKHNNMASFVRQLNMYGFRKVVHIESGIIKQERDGPVEFQHPYFKQGQDDLLENIKRKVSSSKPEENKIRQEDLTKIISSAQKVQIKQETIESRLSELKSENESLWKEVSELRAKHAQQQQVIRKIVQFIVTLVQNNQLVSLKRKRPLLLNTNGAPKKNLYQHIVKEPTDNHHHKVPHSRTEGLKSRERISDDIIIYDVTDDNVDEENIPVIPETNEDVVVDSSNQYPDIVIVEDDNEDEYAPVIQSGEQSEPAREPLRVGSAGSSSPLMSSAVQLNGSSSLTSEDPVTMMDSILNDNINLLGKVELLDYLDSIDCSLEDFQAMLSGRQFSIDPDLLVDSENKGLEATKSSVVQHVSEEGRKSKSKPDKQLIQYTAFPLLAFLDGNSASAIEQGSTTASSEVVPSVDKPIEVDELLDSSLDPEPTQSKLVRLEPLTEAEASEATLFYLCELAPAPLDSDMPLLDS*"
     primer          2623..2646
                     /label="oST788"
                     /note="sequence: TACCCATACGATGTTCCAGATTAC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             2650..2718
                     /label="3xFLAG"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             2728..3618
                     /label="HaloTag"
                     /ApEinfo_revcolor="#333399"
                     /ApEinfo_fwdcolor="#333399"
     primer          2742..2762
                     /label="oST450"
                     /note="sequence: TACTGGCTTTCCATTCGACCC"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          2842..2880
                     /label="oST779"
                     /note="sequence: CACGGTAACCCGACCTCCTCCTACGTGTGGCGCAACATC"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          3509..3528
                     /label="oST183"
                     /note="sequence: ACTGCAAGGCTGTGGACATC"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(3533..3551)
                     /label="oST451"
                     /note="sequence: TGCAGCAGATTCAGACCCG"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          3544..3563
                     /label="Halo-H2.qPCR.fwd"
                     /note="sequence: CTGCTGCAAGAAGACAACCC"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             3631..3651
                     /label="TEV protease site"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     primer          3658..3685
                     /label="Maybe primer 2"
                     /note="sequence: TCTAGAatgaagcagagttccaacgtgc"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     exon            3663..3756
                     /label="exon"
                     /ApEinfo_revcolor="#708090"
                     /ApEinfo_fwdcolor="#708090"
     gene            3663..5217
                     /label="Hsf2"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    3664..3996
                     /label="HSF2DBD"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             3664..5217
                     /label="Hsf2"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     primer          complement(3705..3724)
                     /label="Halo-H2.qPCR.rev"
                     /note="sequence: tttcctccacaagcgtccac"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     exon            3757..3865
                     /label="exon"
                     /ApEinfo_revcolor="#708090"
                     /ApEinfo_fwdcolor="#708090"
     exon            3866..3993
                     /label="exon"
                     /ApEinfo_revcolor="#708090"
                     /ApEinfo_fwdcolor="#708090"
     misc_feature    3985..4029
                     /label="NLS1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     exon            3994..4118
                     /label="exon"
                     /ApEinfo_revcolor="#708090"
                     /ApEinfo_fwdcolor="#708090"
     misc_feature    4018..4239
                     /label="HR-A/B "
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          4030..4055
                     /label="oST204"
                     /note="sequence: caggaagatttaacaaaaattattag"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     exon            4119..4194
                     /label="exon"
                     /ApEinfo_revcolor="#708090"
                     /ApEinfo_fwdcolor="#708090"
     exon            4195..4256
                     /label="exon"
                     /ApEinfo_revcolor="#708090"
                     /ApEinfo_fwdcolor="#708090"
     misc_feature    4246..4290
                     /label="NLS2"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     exon            4257..4344
                     /label="exon"
                     /ApEinfo_revcolor="#708090"
                     /ApEinfo_fwdcolor="#708090"
     exon            4345..4493
                     /label="exon"
                     /ApEinfo_revcolor="#708090"
                     /ApEinfo_fwdcolor="#708090"
     exon            4494..4730
                     /label="exon"
                     /ApEinfo_revcolor="#708090"
                     /ApEinfo_fwdcolor="#708090"
     exon            4731..4836
                     /label="exon"
                     /ApEinfo_revcolor="#708090"
                     /ApEinfo_fwdcolor="#708090"
     exon            4837..4921
                     /label="exon"
                     /ApEinfo_revcolor="#708090"
                     /ApEinfo_fwdcolor="#708090"
     STS             4910..5217
                     /label="Hsf2"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     exon            4922..5217
                     /label="exon"
                     /ApEinfo_revcolor="#708090"
                     /ApEinfo_fwdcolor="#708090"
     STS             4997..5115
                     /label="Hsf2"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    5220..5225
                     /label="EX-MCS"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(5226..5241)
                     /label="oST614"
                     /note="sequence: GGGGAGGGAGAGGGGC"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     RBS             5226..5799
                     /label="IRES"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(5308..5327)
                     /label="oST086"
                     /note="sequence: AAGACGGCAATATGGTGGAA"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             5808..6602
                     /label="NeoR/KanR"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          5848..5868
                     /label="oST449"
                     /note="sequence: CTTGGGTGGAGAGGCTATTCG"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(6581..6620)
                     /label="oST660"
                     /note="sequence: CAGAGGTTGATTGTCGACTCAGAAGAACTCGTCAAGAAGG"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(6581..6620)
                     /label="oST660"
                     /note="sequence: CAGAGGTTGATTGTCGACTCAGAAGAACTCGTCAAGAAGG"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          6603..6628
                     /label="oST661 copy"
                     /note="sequence: GTCGACAATCAACCTCTGGATTACAA"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     polyA_signal    6724..6845
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(7605..7620)
                     /label="IR (inverted repeat) truncated?"
                     /ApEinfo_revcolor="#339966"
                     /ApEinfo_fwdcolor="#339966"
     misc_feature    complement(7605..7623)
                     /label="IR (inverted repeat)"
                     /ApEinfo_revcolor="#339966"
                     /ApEinfo_fwdcolor="#339966"
     misc_feature    complement(7627..7639)
                     /label="PB Terminal repeat"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     complement(8130..8146)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
     protein_bind    8154..8170
                     /label="lac operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     promoter        complement(8178..8184)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        complement(8185..8202)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        complement(8203..8208)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind    8223..8244
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     rep_origin      complement(8532..9119)
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             complement(9290..649)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
ORIGIN
        1 ACCCACGCTC ACCGGTTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC
       61 GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG
      121 CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA
      181 TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA
      241 GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA
      301 TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA
      361 ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA
      421 AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG
      481 ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG
      541 GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG
      601 CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG
      661 GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC
      721 TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA
      781 TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG
      841 TGCCACCTAA ATTGTAAGCG TTAATATTTT GTTAAAATTC GCGTTAAATT TTTGTTAAAT
      901 CAGCTCATTT TTTAACCAAT AGGCCGAAAT CGGCAAAATC CCTTATAAAT CAAAAGAATA
      961 GACCGAGATA GGGTTGAGTG TTGTTCCAGT TTGGAACAAG AGTCCACTAT TAAAGAACGT
     1021 GGACTCCAAC GTCAAAGGCG AAAAACCGTC TATCAGGGCG ATGGCCCACT ACGTGAACCA
     1081 TCACCCTAAT CAAGTTTTTT GGGGTCGAGG TGCCGTAAAG CACTAAATCG GAACCCTAAA
     1141 GGGAGCCCCC GATTTAGAGC TTGACGGGGA AAGCCGGCGA ACGTGGCGAG AAAGGAAGGG
     1201 AAGAAAGCGA AAGGAGCGGG CGCTAGGGCG CTGGCAAGTG TAGCGGTCAC GCTGCGCGTA
     1261 ACCACCACAC CCGCCGCGCT TAATGCGCCG CTACAGGGCG CGTCCCATTC GCCATTCAGG
     1321 CTGCGCAACT GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG CCAGCTGGCG
     1381 AAAGGGGGAT GTGCTGCAAG GCGATTAAGT TGGGTAACGC CAGGGTTTCC CAGTCACGAC
     1441 GTTGTAAAAC GACGGCCAGT GAGCGCGCCT CGTTCATTCA CGTTTTTGAA CCCGTGGAGG
     1501 ACGGGCAGAC TCGCGGTGCA AATGTGTTTT ACAGCGTGAT GGAGCAGATG AAGATGCTCG
     1561 ACACGCTGCA GAACACGCAG CTAGATTAAC CCTAGAAAGA TAATCATATT GTGACGTACG
     1621 TTAAAGATAA TCATGTGTAA AATTGACGCA TGTGTTTTAT CGGTCTGTAT ATCGAGGTTT
     1681 ATTTATTAAT TTGAATAGAT ATTAAGTTTT ATTATATTTA CACTTACATA CTAATAATAA
     1741 ATTCAACAAA CAATTTATTT ATGTTTATTT ATTTATTAAA AAAAACAAAA ACTCAAAATT
     1801 TCTTCTATAA AGTAACAAAA CTTTTATGAG GGACAGCCCC CCCCCAAAGC CCCCAGGGAT
     1861 GTAATTACGT CCCTCCCCCG CTAGGGGGCA GCAGCGAGCC GCCCGGGGCT CCGCTCCGGT
     1921 CCGGCGCTCC CCCCGCATCC CCGAGCCGGC AGCGTGCGGG GACAGCCCGG GCACGGGGAA
     1981 GGTGGCACGG GATCGCTTTC CTCTGAACGC TTCTCGCTGC TCTTTGAGCC TGCAGACACC
     2041 TGGGGGGATA CGGGGAAAAG GCCTCCACGG CCAAGGATCT GCGATCGCTC CGGTGCCCGT
     2101 CAGTGGGCAG AGCGCACATC GCCCACAGTC CCCGAGAAGT TGGGGGGAGG GGTCGGCAAT
     2161 TGAACGGGTG CCTAGAGAAG GTGGCGCGGG GTAAACTGGG AAAGTGATGT CGTGTACTGG
     2221 CTCCGCCTTT TTCCCGAGGG TGGGGGAGAA CCGTATATAA GTGCAGTAGT CGCCGTGAAC
     2281 GTTCTTTTTC GCAACGGGTT TGCCGCCAGA ACACAGCTGA AGCTTCGAGG GGCTCGCATC
     2341 TCTCCTTCAC GCGCCCGCCG CCCTACCTGA GGCCGCCATC CACGCCGGTT GAGTCGCGTT
     2401 CTGCCGCCTC CCGCCTGTGG TGCCTCCTGA ACTGCGTCCG CCGTCTAGGT AAGTTTAAAG
     2461 CTCAGGTCGA GACCGGGCCT TTGTCCGGCG CTCCCTTGGA GCCTACCTAG ACTCAGCCGG
     2521 CTCTCCACGC TTTGCCTGAC CCTGCTTGCT CAACTCTACG TCTTTGTTTC GTTTTCTGTT
     2581 CTGCGCCGTT ACAGATCCAA GCTGTGACCG GCGCCTACTa tgTACCCATA CGATGTTCCA
     2641 GATTACGCTA TGGACTACAA AGACCATGAC GGTGATTATA AAGATCATGA CATCGATTAC
     2701 AAGGATGACG ATGACAAGCT TGGCTCCATG GCAGAAATCG GTACTGGCTT TCCATTCGAC
     2761 CCCCATTATG TGGAAGTCCT GGGCGAGCGC ATGCACTACG TCGATGTTGG TCCGCGCGAT
     2821 GGCACCCCTG TGCTGTTCCT GCACGGTAAC CCGACCTCCT CCTACGTGTG GCGCAACATC
     2881 ATCCCGCATG TTGCACCGAC CCATCGCTGC ATTGCTCCAG ACCTGATCGG TATGGGCAAA
     2941 TCCGACAAAC CAGACCTGGG TTATTTCTTC GACGACCACG TCCGCTTCAT GGATGCCTTC
     3001 ATCGAAGCCC TGGGTCTGGA AGAGGTCGTC CTGGTCATTC ACGACTGGGG CTCCGCTCTG
     3061 GGTTTCCACT GGGCCAAGCG CAATCCAGAG CGCGTCAAAG GTATTGCATT TATGGAGTTC
     3121 ATCCGCCCTA TCCCGACCTG GGACGAATGG CCAGAATTTG CCCGCGAGAC CTTCCAGGCC
     3181 TTCCGCACCA CCGACGTCGG CCGCAAGCTG ATCATCGATC AGAACGTTTT TATCGAGGGT
     3241 ACGCTGCCGA TGGGTGTCGT CCGCCCGCTG ACTGAAGTCG AGATGGACCA TTACCGCGAG
     3301 CCGTTCCTGA ATCCTGTTGA CCGCGAGCCA CTGTGGCGCT TCCCAAACGA GCTGCCAATC
     3361 GCCGGTGAGC CAGCGAACAT CGTCGCGCTG GTCGAAGAAT ACATGGACTG GCTGCACCAG
     3421 TCCCCTGTCC CGAAGCTGCT GTTCTGGGGC ACCCCAGGCG TTCTGATCCC ACCGGCCGAA
     3481 GCCGCTCGCC TGGCCAAAAG CCTGCCTAAC TGCAAGGCTG TGGACATCGG CCCGGGTCTG
     3541 AATCTGCTGC AAGAAGACAA CCCGGACCTG ATCGGCAGCG AGATCGCGCG CTGGCTGTCG
     3601 ACGCTCGAGA TTTCCGGCGA GCCAACCACT GAGGATCTGT ACTTTCAGAG CGATAACTCT
     3661 AGAatgaagc agagttccaa cgtgccggct ttcctcagca agctgtggac gcttgtggag
     3721 gaaacccaca ccaacgagtt catcacctgg agtcagaatg gacaaagttt tctggtcttg
     3781 gatgagcaaa gatttgcaaa ggaaattctt cctaagtact tcaaacacaa taacatggcg
     3841 agctttgtga gacaactaaa tatgtatggc ttccgaaaag tagtgcatat cgaatctgga
     3901 attatcaaac aggaaagaga tggccctgtt gaatttcagc atccttattt caagcaaggc
     3961 caggatgacc tgttggagaa cattaaaagg aaggtttcat cttcaaaacc agaggaaaat
     4021 aaaattcgtc aggaagattt aacaaaaatt attagtagtg ctcagaaggt tcaaataaaa
     4081 caagaaacta ttgagtccag gctttcagaa ttaaaaagtg agaatgaatc cctttggaag
     4141 gaggtgtcag aactaagagc aaagcatgcc cagcagcaac aagttattcg gaagattgtc
     4201 cagtttattg ttacattggt tcagaataat caacttgtga gtttaaaacg taaaaggcct
     4261 ctacttctaa acacaaatgg agccccaaag aagaatctat atcagcacat agtcaaagaa
     4321 ccaactgata atcaccatca taaagttcca cacagcagga ctgaaggttt aaagtcaaga
     4381 gaacggattt cagatgacat aattatttat gatgttactg acgataatgt ggatgaagaa
     4441 aatattccag ttattccaga aacaaatgag gatgttgtag tggattcctc caaccagtat
     4501 cctgacattg tcattgttga agatgacaac gaggatgagt atgctcctgt cattcagagt
     4561 ggagagcaga gtgaaccagc cagagaaccc ttacgtgtgg ggagtgctgg cagcagcagc
     4621 cctctcatgt ctagtgctgt ccagctaaac ggctcctcca gtctgacctc agaagaccct
     4681 gtgaccatga tggactccat tctgaatgac aacattaacc tgttaggaaa ggttgagctg
     4741 ttggattacc ttgacagtat tgattgcagt ttagaggact tccaagctat gctctcagga
     4801 agacagttta gcatagaccc agatcttctg gttgattctg agaataaggg actagaagct
     4861 accaagagca gtgttgttca acatgtgtca gaagagggaa gaaaatctaa atccaagcca
     4921 gacaaacaac ttatccagta tactgccttt ccacttcttg cattcctgga tgggaactct
     4981 gcatctgcta ttgaacaggg gagtacaact gcatcgtcag aagttgtgcc ttctgtagat
     5041 aaacccatag aagtcgatga gctcctggat agcagcctgg atccagaacc gacccagagt
     5101 aagcttgtcc gcctggaacc attgactgaa gcggaagcta gtgaagccac actcttctat
     5161 ttatgtgaac ttgctcctgc acctctggat agtgatatgc cgcttttaga tagttaaGCG
     5221 GCCGCGCCCC TCTCCCTCCC CCCCCCCTAA CGTTACTGGC CGAAGCCGCT TGGAATAAGG
     5281 CCGGTGTGCG TTTGTCTATA TGTTATTTTC CACCATATTG CCGTCTTTTG GCAATGTGAG
     5341 GGCCCGGAAA CCTGGCCCTG TCTTCTTGAC GAGCATTCCT AGGGGTCTTT CCCCTCTCGC
     5401 CAAAGGAATG CAAGGTCTGT TGAATGTCGT GAAGGAAGCA GTTCCTCTGG AAGCTTCTTG
     5461 AAGACAAACA ACGTCTGTAG CGACCCTTTG CAGGCAGCGG AACCCCCCAC CTGGCGACAG
     5521 GTGCCTCTGC GGCCAAAAGC CACGTGTATA AGATACACCT GCAAAGGCGG CACAACCCCA
     5581 GTGCCACGTT GTGAGTTGGA TAGTTGTGGA AAGAGTCAAA TGGCTCTCCT CAAGCGTATT
     5641 CAACAAGGGG CTGAAGGATG CCCAGAAGGT ACCCCATTGT ATGGGATCTG ATCTGGGGCC
     5701 TCGGTGCACA TGCTTTACAT GTGTTTAGTC GAGGTTAAAA AAACGTCTAG GCCCCCCGAA
     5761 CCACGGGGAC GTGGTTTTCC TTTGAAAAAC ACGATAATAC CTCCGGAATG ATTGAACAAG
     5821 ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG
     5881 CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC
     5941 CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG
     6001 CGCGGCTATC GTGGCTGGCC GCGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA
     6061 CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT
     6121 CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA
     6181 CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC
     6241 GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC
     6301 TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GAGGATCTCG
     6361 TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG
     6421 GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA
     6481 CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG
     6541 GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT
     6601 GAGTCGACAA TCAACCTCTG GATTACAAAA TTTGTGAAAG ATTGACTGGT ATTCTTAACT
     6661 ATGTTGCTCC TTTTACGCTA TGTGGATACG CTGCTTTAAT GCCTTTGTAT CATGCGTTAA
     6721 CTAAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC
     6781 ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA
     6841 TCTTATCATG TCTGGAATTG ACTCAAATGA TGTCAATTAG TCTATCAGAA GCTCATCTGG
     6901 TCTCCCTTCC GGGGGACAAG ACATCCCTGT TTAATATTTA AACAGCAGTG TTCCCAAACT
     6961 GGGTTCTTAT ATCCCTTGCT CTGGTCAACC AGGTTGCAGG GTTTCCTGTC CTCACAGGAA
     7021 CGAAGTCCCT AAAGAAACAG TGGCAGCCAG GTTTAGCCCC GGAATTGACT GGATTCCTTT
     7081 TTTAGGGCCC ATTGGTATGG CTTTTTCCCC GTATCCCCCC AGGTGTCTGC AGGCTCAAAG
     7141 AGCAGCGAGA AGCGTTCAGA GGAAAGCGAT CCCGTGCCAC CTTCCCCGTG CCCGGGCTGT
     7201 CCCCGCACGC TGCCGGCTCG GGGATGCGGG GGGAGCGCCG GACCGGAGCG GAGCCCCGGG
     7261 CGGCTCGCTG CTGCCCCCTA GCGGGGGAGG GACGTAATTA CATCCCTGGG GGCTTTGGGG
     7321 GGGGGCTGTC CCTGATATCT ATAACAAGAA ATATATATAT AATAAGTTAT CACGTAAGTA
     7381 GAACATGAAA TAACAATATA ATTATCGTAT GAGTTAATCT TAAAGTCACG TAAAAGATAA
     7441 TCATGCGTCA TTTTGACTCA CGCGGTCGTT ATAGTTCAAA ATCAGTGACA CTTACCGCAT
     7501 TGACAAGCAC GCCTCACGGG AGCTCCAAGC GGCGACTGAG ATGTCCTAAA TGCACAGCGA
     7561 CGGATTCGCG CTATTTAGAA AGAGAGAGCA ATATTTCAAG AATGCATGCG TCAATTTTAC
     7621 GCAGACTATC TTTCTAGGGT TAATCTAGCT GCATCAGGAT CATATCGTCG GGTCTTTTTT
     7681 CCGGCTCAGT CATCGCCCAA GCTGGCGCTA TCTGGGCATC GGGGAGGAAG AAGCCCGTGC
     7741 CTTTTCCCGC GAGGTTGAAG CGGCATGGAA AGAGTTTGCC GAGGATGACT GCTGCTGCAT
     7801 TGACGTTGAG CGAAAACGCA CGTTTACCAT GATGATTCGG GAAGGTGTGG CCATGCACGC
     7861 CTTTAACGGT GAACTGTTCG TTCAGGCCAC CTGGGATACC AGTTCGTCGC GGCTTTTCCG
     7921 GACACAGTTC CGGATGGTCA GCCCGAAGCG CATCAGCAAC CCGAACAATA CCGGCGACAG
     7981 CCGGAACTGC CGTGCCGGTG TGCAGATTAA TGACAGCGGT GCGGCGCTGG GATATTACGT
     8041 CAGCGAGGAC GGGTATCCTG GCTGGATGCC GCAGAAATGG ACATGGATAC CCCGTGAGTT
     8101 ACCCGGCGGG CGCGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT
     8161 CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC
     8221 TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA
     8281 AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT
     8341 ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG
     8401 CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC
     8461 GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG
     8521 TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA
     8581 AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC
     8641 TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC
     8701 CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG
     8761 GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC
     8821 TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA
     8881 GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG
     8941 AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG
     9001 AAGCCAGTTA CCTTCGGAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG
     9061 GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG
     9121 AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG
     9181 GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT
     9241 GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT
     9301 TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC
     9361 TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA
     9421 TGATACCGCG AG
//