LOCUS       pSTL040_TRF2-HA         7433 bp ds-DNA     circular     17-MAR-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     primer_bind     186..202
                     /label="M13 fwd"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
     misc_feature    829..1352
                     /label="EF1a prom"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     promoter        847..1058
                     /label="EF-1α core promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     LTR             1071..1339
                     /label="5' LTR (truncated)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    1361..1968
                     /label="EX-MCS"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    1367..1924
                     /label="mTRF2 cDNA"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             1367..1954
                     /label="Translation 1367-1954"
                     /translation="MDADSDVALDILITNVVCVFRTRCHLNLRKIALEGANVIYKRDVGKVLMKLRKPRITATIWSSGKIICTGATSEEEAKFGARRLARSLQKLGFQVIFTDFKVVNVLAVCNMPFEIRLPEFTKNNRPHASYEPELHPAVCYRIKSLRATLQIFSTGSITVTGPNVKAVATAVEQIYPFVFESRKEILYPYDVPDYA*"
     misc_feature    1925..1951
                     /label="HA tag"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     RBS             1969..2542
                     /label="IRES"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             2551..3345
                     /label="NeoR/KanR"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     polyA_signal    3467..3588
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     complement(4873..4889)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
     protein_bind    4897..4913
                     /label="lac operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     promoter        complement(4921..4927)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        complement(4928..4945)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        complement(4946..4951)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind    4966..4987
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     rep_origin      complement(5275..5862)
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             complement(6825..6893)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     promoter        complement(6894..6998)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     rep_origin      complement(7024..45)
                     /label="f1 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 TAACCACCAC ACCCGCCGCG CTTAATGCGC CGCTACAGGG CGCGTCCCAT TCGCCATTCA
       61 GGCTGCGCAA CTGTTGGGAA GGGCGATCGG TGCGGGCCTC TTCGCTATTA CGCCAGCTGG
      121 CGAAAGGGGG ATGTGCTGCA AGGCGATTAA GTTGGGTAAC GCCAGGGTTT CCCAGTCACG
      181 ACGTTGTAAA ACGACGGCCA GTGAGCGCGC CTCGTTCATT CACGTTTTTG AACCCGTGGA
      241 GGACGGGCAG ACTCGCGGTG CAAATGTGTT TTACAGCGTG ATGGAGCAGA TGAAGATGCT
      301 CGACACGCTG CAGAACACGC AGCTAGATTA ACCCTAGAAA GATAATCATA TTGTGACGTA
      361 CGTTAAAGAT AATCATGTGT AAAATTGACG CATGTGTTTT ATCGGTCTGT ATATCGAGGT
      421 TTATTTATTA ATTTGAATAG ATATTAAGTT TTATTATATT TACACTTACA TACTAATAAT
      481 AAATTCAACA AACAATTTAT TTATGTTTAT TTATTTATTA AAAAAAACAA AAACTCAAAA
      541 TTTCTTCTAT AAAGTAACAA AACTTTTATG AGGGACAGCC CCCCCCCAAA GCCCCCAGGG
      601 ATGTAATTAC GTCCCTCCCC CGCTAGGGGG CAGCAGCGAG CCGCCCGGGG CTCCGCTCCG
      661 GTCCGGCGCT CCCCCCGCAT CCCCGAGCCG GCAGCGTGCG GGGACAGCCC GGGCACGGGG
      721 AAGGTGGCAC GGGATCGCTT TCCTCTGAAC GCTTCTCGCT GCTCTTTGAG CCTGCAGACA
      781 CCTGGGGGGA TACGGGGAAA AGGCCTCCAC GGCCAAGGAT CTGCGATCGC TCCGGTGCCC
      841 GTCAGTGGGC AGAGCGCACA TCGCCCACAG TCCCCGAGAA GTTGGGGGGA GGGGTCGGCA
      901 ATTGAACGGG TGCCTAGAGA AGGTGGCGCG GGGTAAACTG GGAAAGTGAT GTCGTGTACT
      961 GGCTCCGCCT TTTTCCCGAG GGTGGGGGAG AACCGTATAT AAGTGCAGTA GTCGCCGTGA
     1021 ACGTTCTTTT TCGCAACGGG TTTGCCGCCA GAACACAGCT GAAGCTTCGA GGGGCTCGCA
     1081 TCTCTCCTTC ACGCGCCCGC CGCCCTACCT GAGGCCGCCA TCCACGCCGG TTGAGTCGCG
     1141 TTCTGCCGCC TCCCGCCTGT GGTGCCTCCT GAACTGCGTC CGCCGTCTAG GTAAGTTTAA
     1201 AGCTCAGGTC GAGACCGGGC CTTTGTCCGG CGCTCCCTTG GAGCCTACCT AGACTCAGCC
     1261 GGCTCTCCAC GCTTTGCCTG ACCCTGCTTG CTCAACTCTA CGTCTTTGTT TCGTTTTCTG
     1321 TTCTGCGCCG TTACAGATCC AAGCTGTGAC CGGCGCCTAC TCTAGAatgg atgcagacag
     1381 tgatgttgca ttggacattt taattacaaa tgtagtctgt gtttttagaa caagatgcca
     1441 tttgaactta aggaagattg ctttggaggg agcaaatgta atttataagc gtgatgttgg
     1501 gaaagtatta atgaagctta gaaaacctag aattacagct acaatttggt cctcaggaaa
     1561 aattatttgc actggagcaa caagtgaaga agaagctaaa tttggtgcca gacgtttagc
     1621 ccgtagtctg cagaaactag gttttcaggt catcttcaca gattttaagg tggtgaatgt
     1681 tttggcagtg tgtaacatgc cctttgagat ccgtttgcca gaatttacaa agaacaatag
     1741 acctcatgcc agctatgaac ctgaacttca tcctgccgtg tgctatcgga taaagtctct
     1801 aagagctaca ttacagatat tttcaacagg aagcatcaca gtgacagggc ccaatgtaaa
     1861 ggctgtggcg actgccgtgg aacagatcta cccgttcgtg tttgaaagca ggaaggagat
     1921 tttaTACCCA TACGATGTTC CAGATTACGC TtaaGGATCC GCGGCCGCGC CCCTCTCCCT
     1981 CCCCCCCCCC TAACGTTACT GGCCGAAGCC GCTTGGAATA AGGCCGGTGT GCGTTTGTCT
     2041 ATATGTTATT TTCCACCATA TTGCCGTCTT TTGGCAATGT GAGGGCCCGG AAACCTGGCC
     2101 CTGTCTTCTT GACGAGCATT CCTAGGGGTC TTTCCCCTCT CGCCAAAGGA ATGCAAGGTC
     2161 TGTTGAATGT CGTGAAGGAA GCAGTTCCTC TGGAAGCTTC TTGAAGACAA ACAACGTCTG
     2221 TAGCGACCCT TTGCAGGCAG CGGAACCCCC CACCTGGCGA CAGGTGCCTC TGCGGCCAAA
     2281 AGCCACGTGT ATAAGATACA CCTGCAAAGG CGGCACAACC CCAGTGCCAC GTTGTGAGTT
     2341 GGATAGTTGT GGAAAGAGTC AAATGGCTCT CCTCAAGCGT ATTCAACAAG GGGCTGAAGG
     2401 ATGCCCAGAA GGTACCCCAT TGTATGGGAT CTGATCTGGG GCCTCGGTGC ACATGCTTTA
     2461 CATGTGTTTA GTCGAGGTTA AAAAAACGTC TAGGCCCCCC GAACCACGGG GACGTGGTTT
     2521 TCCTTTGAAA AACACGATAA TACCTCCGGA ATGATTGAAC AAGATGGATT GCACGCAGGT
     2581 TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC
     2641 TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG
     2701 ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG
     2761 GCCGCGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC
     2821 TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC
     2881 GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC
     2941 TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC
     3001 GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG
     3061 TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT
     3121 GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC
     3181 CGGCTGGGTG TGGCGGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA
     3241 GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT
     3301 TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGAGTCGA CAATCAACCT
     3361 CTGGATTACA AAATTTGTGA AAGATTGACT GGTATTCTTA ACTATGTTGC TCCTTTTACG
     3421 CTATGTGGAT ACGCTGCTTT AATGCCTTTG TATCATGCGT TAACTAAACT TGTTTATTGC
     3481 AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT
     3541 TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGGAA
     3601 TTGACTCAAA TGATGTCAAT TAGTCTATCA GAAGCTCATC TGGTCTCCCT TCCGGGGGAC
     3661 AAGACATCCC TGTTTAATAT TTAAACAGCA GTGTTCCCAA ACTGGGTTCT TATATCCCTT
     3721 GCTCTGGTCA ACCAGGTTGC AGGGTTTCCT GTCCTCACAG GAACGAAGTC CCTAAAGAAA
     3781 CAGTGGCAGC CAGGTTTAGC CCCGGAATTG ACTGGATTCC TTTTTTAGGG CCCATTGGTA
     3841 TGGCTTTTTC CCCGTATCCC CCCAGGTGTC TGCAGGCTCA AAGAGCAGCG AGAAGCGTTC
     3901 AGAGGAAAGC GATCCCGTGC CACCTTCCCC GTGCCCGGGC TGTCCCCGCA CGCTGCCGGC
     3961 TCGGGGATGC GGGGGGAGCG CCGGACCGGA GCGGAGCCCC GGGCGGCTCG CTGCTGCCCC
     4021 CTAGCGGGGG AGGGACGTAA TTACATCCCT GGGGGCTTTG GGGGGGGGCT GTCCCTGATA
     4081 TCTATAACAA GAAATATATA TATAATAAGT TATCACGTAA GTAGAACATG AAATAACAAT
     4141 ATAATTATCG TATGAGTTAA TCTTAAAGTC ACGTAAAAGA TAATCATGCG TCATTTTGAC
     4201 TCACGCGGTC GTTATAGTTC AAAATCAGTG ACACTTACCG CATTGACAAG CACGCCTCAC
     4261 GGGAGCTCCA AGCGGCGACT GAGATGTCCT AAATGCACAG CGACGGATTC GCGCTATTTA
     4321 GAAAGAGAGA GCAATATTTC AAGAATGCAT GCGTCAATTT TACGCAGACT ATCTTTCTAG
     4381 GGTTAATCTA GCTGCATCAG GATCATATCG TCGGGTCTTT TTTCCGGCTC AGTCATCGCC
     4441 CAAGCTGGCG CTATCTGGGC ATCGGGGAGG AAGAAGCCCG TGCCTTTTCC CGCGAGGTTG
     4501 AAGCGGCATG GAAAGAGTTT GCCGAGGATG ACTGCTGCTG CATTGACGTT GAGCGAAAAC
     4561 GCACGTTTAC CATGATGATT CGGGAAGGTG TGGCCATGCA CGCCTTTAAC GGTGAACTGT
     4621 TCGTTCAGGC CACCTGGGAT ACCAGTTCGT CGCGGCTTTT CCGGACACAG TTCCGGATGG
     4681 TCAGCCCGAA GCGCATCAGC AACCCGAACA ATACCGGCGA CAGCCGGAAC TGCCGTGCCG
     4741 GTGTGCAGAT TAATGACAGC GGTGCGGCGC TGGGATATTA CGTCAGCGAG GACGGGTATC
     4801 CTGGCTGGAT GCCGCAGAAA TGGACATGGA TACCCCGTGA GTTACCCGGC GGGCGCGCTT
     4861 GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA
     4921 CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT
     4981 CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT
     5041 GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC
     5101 TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA
     5161 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG
     5221 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA
     5281 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA
     5341 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC
     5401 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC
     5461 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT
     5521 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG
     5581 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG
     5641 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA
     5701 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG
     5761 AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT
     5821 GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT
     5881 TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA
     5941 TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC
     6001 TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT
     6061 ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA
     6121 ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA
     6181 CGCTCACCGG TTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA
     6241 AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA
     6301 GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG
     6361 GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA
     6421 GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT
     6481 GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT
     6541 CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA
     6601 TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT
     6661 ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA
     6721 AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC
     6781 AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG
     6841 CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC
     6901 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT
     6961 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
     7021 CCTAAATTGT AAGCGTTAAT ATTTTGTTAA AATTCGCGTT AAATTTTTGT TAAATCAGCT
     7081 CATTTTTTAA CCAATAGGCC GAAATCGGCA AAATCCCTTA TAAATCAAAA GAATAGACCG
     7141 AGATAGGGTT GAGTGTTGTT CCAGTTTGGA ACAAGAGTCC ACTATTAAAG AACGTGGACT
     7201 CCAACGTCAA AGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG AACCATCACC
     7261 CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA AATCGGAACC CTAAAGGGAG
     7321 CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG GCGAGAAAGG AAGGGAAGAA
     7381 AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCG
//