LOCUS       Exported                3099 bp ds-DNA     circular SYN 31-AUG-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Mar 22, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        62..90
                     /label=AmpR_promoter
     primer_bind     complement(75..94)
                     /label=AmpR_R
     gene            132..992
                     /gene="Ampicillin"
                     /label=Ampicillin
     CDS             132..992
                     /codon_start=1
                     /label=ORF frame 3
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1147..1766
                     /label=pBR322_origin
     primer_bind     1692..1711
                     /label=pBR322_ori_F
     primer_bind     1798..1819
                     /label=Seq-primer
     primer_bind     1799..1841
                     /label=IP02-F
     primer_bind     1819..1829
                     /label=P02_F
     primer_bind     1819..1829
                     /label=IP01-F
     primer_bind     1821..1840
                     /label=GG01_core-F
     polyA_signal    1830..1878
                     /note="synthetic polyadenylation signal"
     misc_feature    1892..1983
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(1960..1983)
                     /label=IP02-R
     primer_bind     complement(1963..1993)
                     /label=P02_R
     primer_bind     complement(1963..1987)
                     /label=IP01-R
     misc_feature    2026..2029
                     /label=RW
     promoter        2042..2075
                     /label=Minimal Ade MLP promoter
                     /label=Minimal Ade MLP
     primer_bind     2096..2124
                     /label=q004-F
     CDS             2107..2826
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     gene            2107..2823
                     /gene="ECFP"
                     /label=ECFP
     primer_bind     2825..2839
                     /label=SV40polyA-F
     primer_bind     2826..2839
                     /label=IHBGpA-F
     primer_bind     complement(2831..2850)
                     /label=q003-4-R
     gene            2843..3064
                     /gene="SV40"
                     /label=SV40
     polyA_signal    2843..3064
                     /gene="SV40"
     polyA_signal    2852..2973
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     2917..2936
                     /label=SV40-polyA-R Addgene
     primer_bind     complement(join(3061..3099,1..8))
                     /label=IHBGpA02-R
     primer_bind     complement(3061..3086)
                     /label=IHBGpA-R
     primer_bind     complement(3061..3086)
                     /label=SV40polyA-R
     primer_bind     complement(3069..3087)
                     /label=GG01_core-R
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gtgcatgcgg agcggccgca ataaaatatc tttattttca ttacatctgt
     1861 gtgttggttt tttgtgtgaa tcgatagtac taacatacgc tctccatcaa aacaaaacga
     1921 aacaaaacaa actagcaaaa taggctgtcc ccagtgcaag tgcaggtgcc agaacatttc
     1981 tctatcgggt accttctaga cagtcgcgtg tagcgaagca aggggcgcgc ccctagggag
     2041 cggggggcta taaaaggggg tgggggcgtt cgtcctgcta tctagcgtcg cgttgaccga
     2101 gctcccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     2161 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     2221 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     2281 cccaccctcg tgaccaccct gacctggggc gtgcagtgct tcgcccgcta ccccgaccac
     2341 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     2401 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     2461 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     2521 gggcacaagc tggagtacaa cgccatcagc gacaacgtct atatcaccgc cgacaagcag
     2581 aagaacggca tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     2641 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     2701 aaccactacc tgagcaccca gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac
     2761 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     2821 aagtaaggcc ggccgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc
     2881 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     2941 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
     3001 tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt
     3061 ggtaaaatcg ataaggatcc ctcgagcctg cagggacgt
//
