LOCUS       Exported               11500 bp ds-DNA     circular SYN 09-AUG-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11500)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Mar 22, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11500
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     1..46
                     /label=   OP_VP64_(+)200nt_Citr_FW
     misc_feature    4..10
                     /label=include in primer
     primer_bind     5..39
                     /label=OP_zeb_Vp64_v2_CasF_BbSI_RW
     misc_feature    5..10
                     /label=Kozak
     misc_feature    11..4388
                     /label=Insert Fragment
     primer_bind     11..61
                     /label=F2_Cas9m4-VP64-Citrine_for_Sox10
     primer_bind     11..31
                     /label=F_Cas9m4-VP64-Citrine_for_Sox10
     misc_feature    11..13
                     /label=Start
     CDS             14..34
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             14..20
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PK"
     misc_feature    47..4147
                     /label=Cas9m4
                     /note="Cas9m4 from Mali et al. 2013 paper 
                     (Church)…S.pyogenes Cas9, 4aa in Mg coordination mutated"
     primer_bind     complement(143..162)
                     /label=R_Cas9m4_start
     primer_bind     complement(267..292)
                     /label=OP_zeb_Vp64_v2_CasF_BbSI_FW
     primer_bind     658..676
                     /label=KCC_2
     primer_bind     complement(863..882)
                     /label=Primer 111
     primer_bind     1295..1314
                     /label=Cas9m4_F
                     /note="Sequencing primer"
     primer_bind     1458..1477
                     /label=KCC_3
     misc_feature    1741
                     /label=A1731g
                     /note="A1731g"
     misc_feature    2203
                     /label=T2193c
                     /note="T2193c"
     primer_bind     2258..2279
                     /label=KCC_4
     primer_bind     2371..2393
                     /label=Primer 99
     primer_bind     complement(2480..2499)
                     /label=Primer 100
     primer_bind     2521..2540
                     /label=x330-cas9 seq 5
                     /note="From Upeka's box ""GRN Tal effector work"""
     primer_bind     3058..3077
                     /label=KCC_5
     primer_bind     3185..3204
                     /label=Primer 101
     primer_bind     3858..3879
                     /label=KCC_6
     primer_bind     4120..4139
                     /label=Primer 110
     CDS             4160..4180
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     4160..4177
                     /label=F_Cas9m4-VP64-Citrine_for_Sox10
     CDS             4205..4354
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     primer_bind     4272..4297
                     /label=OP_Vp64_gen_nes2_FW
     primer_bind     4278..4297
                     /label=F_VP64
     primer_bind     complement(4345..4389)
                     /label=R_Cas9m4-VP64_in_pGEM-T
     primer_bind     complement(4362..4400)
                     /label=OP_Zeb_Ub_dCas9_Vp64_Fr1_BbSI_RW
     misc_feature    4373..4381
                     /label=GSG linker
                     /note="GSG linker"
     CDS             4382..4435
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(4397..4415)
                     /label=R_T2A
     CDS             4442..5155
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     misc_feature    4442..4459
                     /label=5&rsquo;-EGFP for GFP Kan cassette
                     /note="5-EGFP for GFP Kan cassette"
     misc_feature    4442..4444
                     /label=T7 promoter
                     /note="T7 promoter"
     primer_bind     4447..4464
                     /label=Primer 3
     primer_bind     complement(4457..4477)
                     /label=OP_8xCTS_seq
     primer_bind     complement(4547..4571)
                     /label=OP_Vp64_gen_nes2_RW
     primer_bind     4554..4573
                     /label=OP_seq_Citrine_FW
     primer_bind     complement(4560..4579)
                     /label=R_citrine
     primer_bind     complement(4621..4648)
                     /label=   OP_VP64_(+)200nt_Citr_RW
     primer_bind     4629..4656
                     /label=   OP_Tol2_(-)200nt_Citr_FW
     misc_feature    4817..4836
                     /label=Cas9_seq10 (from Michael)
     primer_bind     4817..4836
                     /label=Cas9_seq10 (from Michael)
     primer_bind     4854..4875
                     /label=eGFP5'
     primer_bind     complement(4884..4904)
                     /label=eGFP3'
     primer_bind     5065..5084
                     /label=F_cerulean
     primer_bind     complement(5137..5155)
                     
     primer_bind     complement(5158..5193)
                     /label=R_GSG-2a-Citrine_in_pMTB2
     promoter        complement(5189..5207)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    5214..5348
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    5358..5361
                     /label=overhang 2
     primer_bind     5410..5465
                     /label=OP_GibAss_Tol2_FW
     primer_bind     complement(5410..5439)
                     /label=OP_zeb_gen_nes1_RW
     misc_feature    5410..5433
                     /label=MCS2
     primer_bind     5410..5432
                     /label=OP_zeb_Vp64_v2_Tol_BbSI_FW
     misc_feature    5440..5589
                     /label=Tol2-R150
                     /note="transposon:T2AL200R150G"
     primer_bind     complement(5647..5663)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5670..5689)
                     /label=﻿OP_Tol2_seq_RW
     rep_origin      5805..6260
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        6286..6390
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6391..7251
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7422..8010
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    8298..8319
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8334..8364
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8372..8388
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8396..8412
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8433..8451
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     8438..8455
                     /label=﻿OP_Tol2_seq_FW
     primer_bind     8488..8504
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    8538..8737
                     /label=Tol2-L200
                     /note="transposon:T2AL200R150G"
     primer_bind     8732..8751
                     /label=OP_zeb_gen_nes1_FW
     misc_feature    8747..8750
                     /label=overhang 1
     promoter        8751..9986
                     /label=beta-actin2 promoter
     misc_feature    9121^9122
                     /label=1_Mutation 1: 1_extra_T
     primer_bind     9166..9192
                     /label=OP_seq_beta_actin_prom_FW
     primer_bind     9871..9890
                     /label=OP_seq_beta_actin_2_prom_FW
     exon            9987..10072
                     /label=beta-actin2 exon1
     intron          10073..11433
                     /label=beta-actin2 intron1
     misc_feature    10252
                     /label=2_Mutation_2: point_mutation
     primer_bind     10575..10595
                     /label=OP_seq_beta_actin_3_prom_FW
     primer_bind     11270..11289
                     /label=OP_seq_beta_actin_4_prom_FW
     exon            11434..11442
                     /label=beta-actin2 exon2
     misc_feature    11437
                     /label=3_Mutation_3: point mutation
     promoter        11452..11474
                     /label=SP6
     promoter        11452..11470
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(join(11458..11500,1..11))
                     /label=OP_GibAss_Tol2_RW
     primer_bind     complement(11460..11494)
                     /label=OP_zeb_Vp64_v2_Tol_BbSI_RW
     primer_bind     complement(join(11480..11500,1..20))
                     /label=   OP_Tol2_(-)200nt_Citr_RW
     primer_bind     join(11494..11500,1..46)
                     /label=OP_GibAss_Vp64_FW
     primer_bind     join(11494..11500,1..46)
                     /label=Gibs_FW
     primer_bind     join(11497..11500,1..39)
                     /label=OP_Zeb_Ub_dCas9_Vp64_Fr1_BbSI_FW
     misc_feature    join(11500,1..3)
                     /label=overhang1
ORIGIN
        1 atccgccacc atgcccaaga agaagaggaa ggtgggaagg gggatggaca agaagtactc
       61 cattgggctc gctatcggca caaacagcgt cggctgggcc gtcattacgg acgagtacaa
      121 ggtgccgagc aaaaaattca aagttctggg caataccgat cgccacagca taaagaagaa
      181 cctcattggc gccctcctgt tcgactccgg ggagacggcc gaagccacgc ggctcaaaag
      241 aacagcacgg cgcagatata cccgcagaaa gaatcggatc tgctacctgc aggagatctt
      301 tagtaatgag atggctaagg tggatgactc tttcttccat aggctggagg agtccttttt
      361 ggtggaggag gataaaaagc acgagcgcca cccaatcttt ggcaatatcg tggacgaggt
      421 ggcgtaccat gaaaagtacc caaccatata tcatctgagg aagaagcttg tagacagtac
      481 tgataaggct gacttgcggt tgatctatct cgcgctggcg catatgatca aatttcgggg
      541 acacttcctc atcgaggggg acctgaaccc agacaacagc gatgtcgaca aactctttat
      601 ccaactggtt cagacttaca atcagctttt cgaagagaac ccgatcaatg catccggagt
      661 tgacgccaaa gcaatcctga gcgctaggct gtccaaatcc cggcggctcg aaaacctcat
      721 cgcacagctc cctggggaga agaagaacgg cctgtttggt aatcttatcg ccctgtcact
      781 cgggctgacc cccaacttta aatctaactt cgacctggcc gaagatgcca agcttcaact
      841 gagcaaagac acctacgatg atgatctcga caatctgctg gcccagatcg gcgaccagta
      901 cgcagacctt tttttggcgg caaagaacct gtcagacgcc attctgctga gtgatattct
      961 gcgagtgaac acggagatca ccaaagctcc gctgagcgct agtatgatca agcgctatga
     1021 tgagcaccac caagacttga ctttgctgaa ggcccttgtc agacagcaac tgcctgagaa
     1081 gtacaaggaa attttcttcg atcagtctaa aaatggctac gccggataca ttgacggcgg
     1141 agcaagccag gaggaatttt acaaatttat taagcccatc ttggaaaaaa tggacggcac
     1201 cgaggagctg ctggtaaagc ttaacagaga agatctgttg cgcaaacagc gcactttcga
     1261 caatggaagc atcccccacc agattcacct gggcgaactg cacgctatcc tcaggcggca
     1321 agaggatttc tacccctttt tgaaagataa cagggaaaag attgagaaaa tcctcacatt
     1381 tcggataccc tactatgtag gccccctcgc ccggggaaat tccagattcg cgtggatgac
     1441 tcgcaaatca gaagagacca tcactccctg gaacttcgag gaagtcgtgg ataagggggc
     1501 ctctgcccag tccttcatcg aaaggatgac taactttgat aaaaatctgc ctaacgaaaa
     1561 ggtgcttcct aaacactctc tgctgtacga gtacttcaca gtttataacg agctcaccaa
     1621 ggtcaaatac gtcacagaag ggatgagaaa gccagcattc ctgtctggag agcagaagaa
     1681 agctatcgtg gacctcctct tcaagacgaa ccggaaagtt accgtgaaac agctcaaaga
     1741 ggactatttc aaaaagattg aatgtttcga ctctgttgaa atcagcggag tggaggatcg
     1801 cttcaacgca tccctgggaa cgtatcacga tctcctgaaa atcattaaag acaaggactt
     1861 cctggacaat gaggagaacg aggacattct tgaggacatt gtcctcaccc ttacgttgtt
     1921 tgaagatagg gagatgattg aagaacgctt gaaaacttac gctcatctct tcgacgacaa
     1981 agtcatgaaa cagctcaaga ggcgccgata tacaggatgg gggcggctgt caagaaaact
     2041 gatcaatggg atccgagaca agcagagtgg aaagacaatc ctggattttc ttaagtccga
     2101 tggatttgcc aaccggaact tcatgcagtt gatccatgat gactctctca cctttaagga
     2161 ggacatccag aaagcacaag tttctggcca gggggacagt ctccacgagc acatcgctaa
     2221 tcttgcaggt agcccagcta tcaaaaaggg aatactgcag accgttaagg tcgtggatga
     2281 actcgtcaaa gtaatgggaa ggcataagcc cgagaatatc gttatcgaga tggcccgaga
     2341 gaaccaaact acccagaagg gacagaagaa cagtagggaa aggatgaaga ggattgaaga
     2401 gggtataaaa gaactggggt cccaaatcct taaggaacac ccagttgaaa acacccagct
     2461 tcagaatgag aagctctacc tgtactacct gcagaacggc agggacatgt acgtggatca
     2521 ggaactggac atcaatcggc tctccgacta cgacgtggct gctatcgtgc cccagtcttt
     2581 tctcaaagat gattctattg ataataaagt gttgacaaga tccgataaag ctagagggaa
     2641 gagtgataac gtcccctcag aagaagttgt caagaaaatg aaaaattatt ggcggcagct
     2701 gctgaacgcc aaactgatca cacaacggaa gttcgataat ctgactaagg ctgaacgagg
     2761 tggcctgtct gagttggata aagccggctt catcaaaagg cagcttgttg agacacgcca
     2821 gatcaccaag cacgtggccc aaattctcga ttcacgcatg aacaccaagt acgatgaaaa
     2881 tgacaaactg attcgagagg tgaaagttat tactctgaag tctaagctgg tctcagattt
     2941 cagaaaggac tttcagtttt ataaggtgag agagatcaac aattaccacc atgcgcatga
     3001 tgcctacctg aatgcagtgg taggcactgc acttatcaaa aaatatccca agcttgaatc
     3061 tgaatttgtt tacggagact ataaagtgta cgatgttagg aaaatgatcg caaagtctga
     3121 gcaggaaata ggcaaggcca ccgctaagta cttcttttac agcaatatta tgaatttttt
     3181 caagaccgag attacactgg ccaatggaga gattcggaag cgaccactta tcgaaacaaa
     3241 cggagaaaca ggagaaatcg tgtgggacaa gggtagggat ttcgcgacag tccggaaggt
     3301 cctgtccatg ccgcaggtga acatcgttaa aaagaccgaa gtacagaccg gaggcttctc
     3361 caaggaaagt atcctcccga aaaggaacag cgacaagctg atcgcacgca aaaaagattg
     3421 ggaccccaag aaatacggcg gattcgattc tcctacagtc gcttacagtg tactggttgt
     3481 ggccaaagtg gagaaaggga agtctaaaaa actcaaaagc gtcaaggaac tgctgggcat
     3541 cacaatcatg gagcgatcaa gcttcgaaaa aaaccccatc gactttctcg aggcgaaagg
     3601 atataaagag gtcaaaaaag acctcatcat taagcttccc aagtactctc tctttgagct
     3661 tgaaaacggc cggaaacgaa tgctcgctag tgcgggcgag ctgcagaaag gtaacgagct
     3721 ggcactgccc tctaaatacg ttaatttctt gtatctggcc agccactatg aaaagctcaa
     3781 agggtctccc gaagataatg agcagaagca gctgttcgtg gaacaacaca aacactacct
     3841 tgatgagatc atcgagcaaa taagcgaatt ctccaaaaga gtgatcctcg ccgacgctaa
     3901 cctcgataag gtgctttctg cttacaataa gcacagggat aagcccatca gggagcaggc
     3961 agaaaacatt atccacttgt ttactctgac caacttgggc gcgcctgcag ccttcaagta
     4021 cttcgacacc accatagaca gaaagcggta cacctctaca aaggaggtcc tggacgccac
     4081 actgattcat cagtcaatta cggggctcta tgaaacaaga atcgacctct ctcagctcgg
     4141 tggagacagc agggctgacc ccaagaagaa gaggaaggtg gaggccagcg gttccggacg
     4201 ggctgacgca ttggacgatt ttgatctgga tatgctggga agtgacgccc tcgatgattt
     4261 tgaccttgac atgcttggtt cggatgccct tgatgacttt gacctcgaca tgctcggcag
     4321 tgacgccctt gatgatttcg acctggacat gctgattaac tctagagata tcggatcagg
     4381 agagggcagg ggaagtcttc taacatgcgg ggacgtggag gaaaatcccg ggcccagatc
     4441 tgtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     4501 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     4561 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     4621 cgtgaccacc ttcggctacg gcctgatgtg cttcgcccgc taccccgacc acatgaagca
     4681 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     4741 caaagacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     4801 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     4861 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     4921 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     4981 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     5041 cctgagctac cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     5101 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaag
     5161 cggccgcgac tctagagtat ccggactccc tatagtgagt cgtattaacc ggtgatccag
     5221 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
     5281 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     5341 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
     5401 aggttttttg atatcccggg tttaaacgca tgcagatcta atactcaagt acaattttaa
     5461 tggagtactt ttttactttt actcaagtaa gattctagcc agatactttt acttttaatt
     5521 gagtaaaatt ttccctaagt acttgtactt tcacttgagt aaaatttttg agtacttttt
     5581 acacctctgt caagaaccat atgccggtac ccaatcgccc tataggacgt cgtattacgc
     5641 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     5701 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     5761 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg
     5821 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     5881 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     5941 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     6001 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     6061 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     6121 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     6181 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     6241 aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     6301 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     6361 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     6421 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     6481 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     6541 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     6601 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     6661 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     6721 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     6781 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     6841 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     6901 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     6961 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     7021 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     7081 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     7141 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     7201 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     7261 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     7321 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     7381 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     7441 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     7501 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     7561 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     7621 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     7681 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     7741 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     7801 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     7861 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     7921 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     7981 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     8041 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     8101 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     8161 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     8221 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     8281 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     8341 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     8401 aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag
     8461 ctggagctcc accgcggtgg cggccgctct agaactagtg gatctgctgg gcttgctgaa
     8521 ggtagggggt caagaaccag aggtgtaaag tacttgagta attttacttg attactgtac
     8581 ttaagtatta tttttgggga tttttacttt acttgagtac aattaaaaat caatactttt
     8641 acttttactt aattacattt ttttagaaaa aaaagtactt tttactcctt acaattttat
     8701 ttacagtcaa aaagtactta ttttttggag atcacttggg cccggctcga gggcctagtc
     8761 attacctata ctcaacgttt actctgagaa acttccatat tttagaggta aaatgctcag
     8821 aattgtaaaa agtcttgtca aaatacttgg aatttgccaa aaacaaaata aaagtcacat
     8881 cacctcattg tccttggtat cagcaagcac ttttaacaca atcagctgtt tttgtttata
     8941 actcccactg aaggccatgt gcattactcc caagttacac agtaaaaagt cacatgtagt
     9001 ttatcatgat cactgctgct caagcacagc tttatctgtt attcagtgtg gcaatatctg
     9061 aaatgtgtgc aaaatattat ttattgtagc tgatcaccaa cactttcagt gttttttttt
     9121 tctactttta atgagaatta tgttcttagg atttatcctt ataaacctct ttaaaaagaa
     9181 atgttatgtc tgcatagaca cgacatgaaa ttatgaacag aaaatttaac tatttaagat
     9241 caagacttga catataacat ttctaatgaa cattaaacct gaggtaggcc cacttgcaaa
     9301 aatgtaactg ctatcactca ctttatacat cattgtgtaa cttgtacaca caaaaatagt
     9361 gaagcaatgt ggcatggaat gcaggtccca ttcattcaaa gttattaaag cactttctta
     9421 aagtgcatta ctactgagct aagaccagct agttgctcat acaacatact acaagtagtg
     9481 tcatcctaat ttggataaaa aaaatgtcca ttttatacat ggaaaagtac tcattagatc
     9541 ccccattgtt tgtattacgg tatttcgtga acacaagagg taaatgacct acagagctgc
     9601 tgttgtgtta gattgaaaac acaacacagg atcatggagg catccatcat tatgacgcat
     9661 tctgccactt gtaactctgc acagactttt tgaaaagttt aattgagtct tggcgttgtc
     9721 gcagaaaaat acgaaaaatt ctgtgttaaa atttgcaaat aaaaattcca gttttatagg
     9781 aaatcgccat ggccttgttc tttaaccagt taagcttccc cttctttcac tctcaagttg
     9841 caagaagcaa gtgtagcaat gtgcacgcga cagccgggtg tgtgacgctg gaccaatcag
     9901 agcacagagc tccgaaagtt taccttttat ggctagagcc gggtatgtgc cgtcatataa
     9961 aagagcgcgc ccagcttttc agcctcactt tgagctcctc cacacgcagc tagtgcggaa
    10021 tatcatctgc ttgtaaccca ttctcttaag tcgacaaccc cccaaaccca aggtgagttg
    10081 gttttacagt ttttaagcgt ttaattatag tttctatcta ttaatgttaa gtaaaatgtg
    10141 taaatggata aatatgattt gatgaagtct ttagacagtt cataacggtc tctttgtagt
    10201 tatctactag gtttgattta taagtggtgg ttcttttgat aatctatgtt acatttaata
    10261 aatattggct gtatcttaac ttttttgtag cgttagtgga tgtggcaggt gagaatgtcg
    10321 gtaatttaac gtgaccagtc gtaggcacga cattgaacat tgaataggcc ggtgtgaaat
    10381 gggtgttaag tcttaatata aagttgtgtt ctggctaatc tctctttaac agccataaat
    10441 gtgattttta actacagttt tgagggtgct ggtgagcatg ttgcacactt gatggatagc
    10501 cggcatggga agttctttgt gcaggcagtg ctgcagcagg gtgtgaccta ctttagctag
    10561 ccggctaacc agctctcatc tgatgtaacg taaaccccat caatctaaca tcggctcatt
    10621 cgtgctttat aagatcgaag atcttgttat tagtaacggg tattggcatt tagttttaac
    10681 ttggttaatt ttctttttgt tatgtgttct atttaaatgt agcctgcagc cccctctgtc
    10741 tttaatgaag ctgaagatta acgttaacgt tagtgaagtg ttgtatccgc tatgaaagat
    10801 ttgaatttta gaggtgtggt ccactttaat aacggctgcg gagttctctg gctgcacttc
    10861 agccgctgtg actgtcgaag cagagcccat cagtttttca ggatctcggt gatttttgag
    10921 gctagactat gaactgaacc gactgagagt gaccgtccag aaatgcttta gtgtgaagtc
    10981 tccgctcacg aggagtccgt ggactgactg cagatctgtg acgcagtaac acccggcggc
    11041 agacacccgt tgaaatgcgg ttgtgtaatt gatcgcaggc gagggtggaa gaggatgtga
    11101 aacttcattt gtgtaaaatt tagggagtgg tcctggtgtg atgaatgtcg aaatctgttc
    11161 ctttttactg agccctacga ctctggctga gtgccacacc gccggcagcc gcaaagcggc
    11221 tcaaaccatt gccttttatg gtaataatga gagaatgcag agggacttcc tttgtctggc
    11281 atatctgagg cgcgcgctgt cactagcgcc caccagcggt cagactgtag aatgcagagc
    11341 aaaacaggaa gttgactcca gatggtcaca tgcttgctga aacgcctctc cctggcagca
    11401 gtgcaattct aacagtttcc ctttctttta cagttcagcc atcctaggcc tatttaggtg
    11461 acactataga agagtacaag ctacttgttc tttttgcagg
//
