LOCUS       OA-1067L_pBac-gR       17008 bp DNA     circular SYN 21-MAR-2023
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17008)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Mar 21, 2023 from SnapGene 6.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..17008
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(95..683)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(854..1714)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1715..1819)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2293..2309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     old_sequence    2319..3026
                     /label=flanking sequence
     misc_binding    complement(3027..4024)
                     /label=piggyBacR
     misc_feature    4080..5062
                     /label=U6_AAEL017774_5
                     /note="U6_AAEL017774_5&sbquo;&Auml;&ocirc;Seq"
     misc_feature    5063..5082
                     /label=sgDsx1
                     /note="sgDsx1 (worked)"
     misc_feature    5083..5168
                     /label=LongScaffold
                     /note="LongScaffold"
     misc_feature    5169..5175
                     /label=Terminator
                     /note="Terminator"
     misc_feature    5176..5773
                     /label=U6_AAEL017774_3
                     /note="U6_AAEL017774_3&sbquo;&Auml;&ocirc;Seq"
     misc_feature    5798..6780
                     /label=U6_AAEL017774_5
                     /note="U6_AAEL017774_5&sbquo;&Auml;&ocirc;Seq"
     misc_feature    6781..6800
                     /label=gRNA-Intersex1
                     /note="gRNA-Intersex1(worked)"
     misc_feature    6801..6886
                     /label=LongScaffold
                     /note="LongScaffold"
     misc_feature    6887..6893
                     /label=Terminator
                     /note="Terminator"
     misc_feature    6894..7491
                     /label=U6_AAEL017774_3
                     /note="U6_AAEL017774_3&sbquo;&Auml;&ocirc;Seq"
     misc_feature    7519..8501
                     /label=U6_AAEL017774_5
                     /note="U6_AAEL017774_5&sbquo;&Auml;&ocirc;Seq"
     misc_feature    8502..8521
                     /label=sgBTub3
                     /label=sgBTub3 (worked)
                     /note="sgBTub3 (worked)"
     misc_feature    8522..8607
                     /label=LongScaffold
                     /note="LongScaffold"
     misc_feature    8608..8614
                     /label=Terminator
                     /note="Terminator"
     misc_feature    8615..9212
                     /label=U6_AAEL017774_3
                     /label=U6_AAEL017774_3&sbquo;&Auml;&ocirc;Seq
                     /note="U6_AAEL017774_3&sbquo;&Auml;&ocirc;Seq"
     misc_feature    9237..10064
                     /label=U6_AAEL017763_5
                     /label=U6_AAEL017763_5&sbquo;&Auml;&ocirc;Seq
                     /note="U6_AAEL017763_5&sbquo;&Auml;&ocirc;Seq"
     misc_feature    10065..10084
                     /label=sgBTub2
                     /label=sgBTub2 (worked)
                     /note="sgBTub2 (worked)"
     misc_feature    10085..10170
                     /label=LongScaffold
                     /note="LongScaffold"
     misc_feature    10171..10177
                     /label=Terminator
                     /note="Terminator"
     misc_feature    10178..10687
                     /label=U6_AAEL017763_3
                     /label=U6_AAEL017763_3&sbquo;&Auml;&ocirc;Seq
                     /note="U6_AAEL017763_3&sbquo;&Auml;&ocirc;Seq"
     misc_feature    10709..10972
                     /label=3xP3 Promoter
                     /note="3xP3 Promoter"
     regulatory      10967..10976
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10973..12404
                     /label=tdTomato
     polyA_signal    12525..12646
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    12659..13641
                     /label=U6_AAEL017774_5
                     /label=U6_AAEL017774_5&sbquo;&Auml;&ocirc;Seq
                     /note="U6_AAEL017774_5&sbquo;&Auml;&ocirc;Seq"
     misc_feature    13642..13661
                     /label=sgDsx2
                     /label=sgDsx2(working)
                     /note="sgDsx2"
     misc_feature    13662..13747
                     /label=LongScaffold
                     /note="LongScaffold"
     misc_feature    13748..13754
                     /label=Terminator
                     /note="Terminator"
     misc_feature    13755..14352
                     /label=U6_AAEL017774_3
                     /label=U6_AAEL017774_3&sbquo;&Auml;&ocirc;Seq
                     /note="U6_AAEL017774_3&sbquo;&Auml;&ocirc;Seq"
     misc_feature    14377..15204
                     /label=U6_AAEL017763_5
                     /label=U6_AAEL017763_5&sbquo;&Auml;&ocirc;Seq
                     /note="U6_AAEL017763_5&sbquo;&Auml;&ocirc;Seq"
     misc_feature    15205..15224
                     /label=sgDsx4
                     /label=sgDsx4(working)
                     /note="sgDsx4"
     misc_feature    15225..15310
                     /label=LongScaffold
                     /note="LongScaffold"
     misc_feature    15311..15317
                     /label=Terminator
                     /note="Terminator"
     misc_feature    15318..15827
                     /label=U6_AAEL017763_3
                     /label=U6_AAEL017763_3&sbquo;&Auml;&ocirc;Seq
                     /note="U6_AAEL017763_3&sbquo;&Auml;&ocirc;Seq"
     misc_binding    15835..16535
                     /label=piggyBacL
     old_sequence    16536..16664
                     /label=flanking seq
     primer_bind     complement(16701..16717)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    16725..16741
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(16749..16779)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    16794..16815
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
ORIGIN
        1 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
       61 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
      121 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
      181 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
      241 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct
      301 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
      361 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
      421 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
      481 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
      541 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
      601 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
      661 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
      721 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
      781 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
      841 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
      901 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
      961 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     1021 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     1081 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     1141 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     1201 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     1261 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     1321 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     1381 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     1441 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     1501 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     1561 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     1621 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     1681 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     1741 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     1801 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     1861 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
     1921 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     1981 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     2041 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     2101 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc
     2161 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     2221 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     2281 agtcacgacg ttgtaaaacg acggccagtg ccaagctttg tttaaaatat aacaaaattg
     2341 tgatcccaca aaatgaagtg gggcaaaatc aaataattaa ctagtgtccg taaacttgtt
     2401 ggtcttcaac tttttgagga acacgttgga cggcaaatcg tgactataac acaagttgat
     2461 ttaataattt tagccaacac gtcgggctgc gtgttttttg cgctctgtgt acacgttgat
     2521 taactggtcg attaaataat ttaatttttg gttcttcttt aaatctgtga tgaaattttt
     2581 taaaataact ttaaattctt cattggtaaa aaatgccacg ttttgcaact tgtgagggtc
     2641 taatatgagg tcaaactcag taggagtttt atccaaaaaa gaaaacatga ttacgtctgt
     2701 acacgaacgc gtattaacgc agagtgcaaa gtataagagg gttaaaaaat atattttacg
     2761 caccatatac gcatcgggtt gatatcgtta atatggatca atttgaacag ttgattaacg
     2821 tgtctctgct caagtctttg atcaaaacgc aaatcgacga aaatgtgtcg gacaatatca
     2881 agtcgatgag cgaaaaacta aaaaggctag aatacgacaa tctcacagac agcgttgaga
     2941 tatacggtat tcacgacagc aggctgaata ataaaaaaat tagaaactat tatttaaccc
     3001 tagaaagata atcatattgt gacgtacgtt aaagataatc atgcgtaaaa ttgacgcatg
     3061 tgttttatcg gtctgtatat cgaggtttat ttattaattt gaatagatat taagttttat
     3121 tatatttaca cttacatact aataataaat tcaacaaaca atttatttat gtttatttat
     3181 ttattaaaaa aaaacaaaaa ctcaaaattt cttctataaa gtaacaaaac ttttaaacat
     3241 tctctctttt acaaaaataa acttattttg tactttaaaa acagtcatgt tgtattataa
     3301 aataagtaat tagcttaact tatacataat agaaacaaat tatacttatt agtcagtcag
     3361 aaacaacttt ggcacatatc aatattatgc tctcgacaaa taactttttt gcattttttg
     3421 cacgatgcat ttgcctttcg ccttatttta gaggggcagt aagtacagta agtacgtttt
     3481 ttcattactg gctcttcagt actgtcatct gatgtaccag gcacttcatt tggcaaaata
     3541 ttagagatat tatcgcgcaa atatctcttc aaagtaggag cttctaaacg cttacgcata
     3601 aacgatgacg tcaggctcat gtaaaggttt ctcataaatt ttttgcgact ttgaaccttt
     3661 tctcccttgc tactgacatt atggctgtat ataataaaag aatttatgca ggcaatgttt
     3721 atcattccgt acaataatgc cataggccac ctattcgtct tcctactgca ggtcatcaca
     3781 gaacacattt ggtctagcgt gtccactccg cctttagttt gattataata cataaccatt
     3841 tgcggtttac cggtactttc gttgatagaa gcatcctcat cacaagatga taataagtat
     3901 accatcttag ctggcttcgg tttatatgag acgagagtaa ggggtccgtc aaaacaaaac
     3961 atcgatgttc ccactggcct ggagcgactg tttttcagta cttccggtat ctcgcgtttg
     4021 tttgatcgca cggttcccac aatggttaat tcgagctcgc ccgggctcct aggagatctt
     4081 catcacaaac gaaactatta taatcgtctt caggggaacg gtagcatcat atctgtcacc
     4141 agtcagtcgg attcctttct ttcggcataa attatgctcg ggtgacgaga cgagttgaaa
     4201 atcccgtaaa ttaaaacata taccacactc gccgtcctag acgacgacga cgacgacgat
     4261 tcccaaggaa ttgaaccctt catttgcatt tcactaccgc tctgagagat gcaaagcgcg
     4321 ccagacagtc gatcgaatct gcagaccaga gagtcacatc aagaatgttg acattccacc
     4381 agaagactct aattattctt cgctcgactg ggaaaactgt taacaactgc tcgagtgtgg
     4441 tcggagcggc tcacagtgcg ttttcccgta gacaaaaatc aaatttcatg gtatttttcg
     4501 gcgataaaca gtaagttaaa gggaacccga atttagtgct atataattta attccactag
     4561 agtttgtatc ctttgataga tacgcgtatt tcgacctcaa ctgcaaggcc gtcgtgtact
     4621 agacttgact aatccagact ggtcttttag ttatgacttc tgtccacatc tccatacatt
     4681 caacgcactg tgcggctgtg ctgtgcgact ccgtcgagtc gaccaacata gttgaaacaa
     4741 attgaatatt taattgatcg ttataggaat ggtgttagat gagtcatcct ttacagtaag
     4801 cacatacagt attataattg aagatcgtcg gcagataggt gtgtagggta gagtatcagc
     4861 aataagttgg gacgtttgac tttttgtagg tagacaaaaa ctaaactttt tttcgcttct
     4921 ctatgtgtgc ccctgggtag cgttccgttc cgattggggt gcgaacgaat gaaatcgccc
     4981 atcgagttga tacgtccatc catcgctaga accgcgttcg ctgtagaaga ctatataaga
     5041 gcagaggcaa gagtagtgaa atagcgacat gcagccattc tggtcttaga gctatgctgg
     5101 aaacagcata gcaagttaag ataaggctag tccgttatca acttgaaaaa gtggcaccga
     5161 gtcggtgctt tttttggtgg ttgttcatcg attcctaata ttgcagtgaa gcagtttgtt
     5221 gtgcatggcg aagataaggt tagggcgagg aatgccttgg aaataagcgg cactgggccg
     5281 tatgaataaa aagcgttgaa ctttactaca tgtagcatct actcaaaaac aaaatccaca
     5341 aagtttacta cacttttggt atgaataaaa accttttcga acatgtagta cctactactt
     5401 taagaaaatt ctattcaaag tacagagtaa tgctgcatgt taagaaaatt ccattcagag
     5461 tgactcttga aatcaataaa atcatgcata tatgccaaat ttgtcactta ttcacgtgac
     5521 aaattatgtg aaaatatgaa tattactaca ttcccgctat gcatcgcgta atcgatcatg
     5581 aaatttatgt tggttatgta actttacagt attcccttta tgtgcatgtg aattttataa
     5641 tgatttgaca acaaggattt tccttagaat tttcgaattt tagctagaaa tctgtatcct
     5701 atgaatgaat cttgagataa cagcaattgt aacattataa agttcctaac attttcaaga
     5761 ttccagtatt gagtcgcgat cgcttaatta accgcggtca tcacaaacga aactattata
     5821 atcgtcttca ggggaacggt agcatcatat ctgtcaccag tcagtcggat tcctttcttt
     5881 cggcataaat tatgctcggg tgacgagacg agttgaaaat cccgtaaatt aaaacatata
     5941 ccacactcgc cgtcctagac gacgacgacg acgacgattc ccaaggaatt gaacccttca
     6001 tttgcatttc actaccgctc tgagagatgc aaagcgcgcc agacagtcga tcgaatctgc
     6061 agaccagaga gtcacatcaa gaatgttgac attccaccag aagactctaa ttattcttcg
     6121 ctcgactggg aaaactgtta acaactgctc gagtgtggtc ggagcggctc acagtgcgtt
     6181 ttcccgtaga caaaaatcaa atttcatggt atttttcggc gataaacagt aagttaaagg
     6241 gaacccgaat ttagtgctat ataatttaat tccactagag tttgtatcct ttgatagata
     6301 cgcgtatttc gacctcaact gcaaggccgt cgtgtactag acttgactaa tccagactgg
     6361 tcttttagtt atgacttctg tccacatctc catacattca acgcactgtg cggctgtgct
     6421 gtgcgactcc gtcgagtcga ccaacatagt tgaaacaaat tgaatattta attgatcgtt
     6481 ataggaatgg tgttagatga gtcatccttt acagtaagca catacagtat tataattgaa
     6541 gatcgtcggc agataggtgt gtagggtaga gtatcagcaa taagttggga cgtttgactt
     6601 tttgtaggta gacaaaaact aaactttttt tcgcttctct atgtgtgccc ctgggtagcg
     6661 ttccgttccg attggggtgc gaacgaatga aatcgcccat cgagttgata cgtccatcca
     6721 tcgctagaac cgcgttcgct gtagaagact atataagagc agaggcaaga gtagtgaaat
     6781 gcgggattcg ctctcgacga gtcttagagc tatgctggaa acagcatagc aagttaagat
     6841 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt tttggtggtt
     6901 gttcatcgat tcctaatatt gcagtgaagc agtttgttgt gcatggcgaa gataaggtta
     6961 gggcgaggaa tgccttggaa ataagcggca ctgggccgta tgaataaaaa gcgttgaact
     7021 ttactacatg tagcatctac tcaaaaacaa aatccacaaa gtttactaca cttttggtat
     7081 gaataaaaac cttttcgaac atgtagtacc tactacttta agaaaattct attcaaagta
     7141 cagagtaatg ctgcatgtta agaaaattcc attcagagtg actcttgaaa tcaataaaat
     7201 catgcatata tgccaaattt gtcacttatt cacgtgacaa attatgtgaa aatatgaata
     7261 ttactacatt cccgctatgc atcgcgtaat cgatcatgaa atttatgttg gttatgtaac
     7321 tttacagtat tccctttatg tgcatgtgaa ttttataatg atttgacaac aaggattttc
     7381 cttagaattt tcgaatttta gctagaaatc tgtatcctat gaatgaatct tgagataaca
     7441 gcaattgtaa cattataaag ttcctaacat tttcaagatt ccagtattga ggccttttgg
     7501 gcccatttaa atcttaagtc atcacaaacg aaactattat aatcgtcttc aggggaacgg
     7561 tagcatcata tctgtcacca gtcagtcgga ttcctttctt tcggcataaa ttatgctcgg
     7621 gtgacgagac gagttgaaaa tcccgtaaat taaaacatat accacactcg ccgtcctaga
     7681 cgacgacgac gacgacgatt cccaaggaat tgaacccttc atttgcattt cactaccgct
     7741 ctgagagatg caaagcgcgc cagacagtcg atcgaatctg cagaccagag agtcacatca
     7801 agaatgttga cattccacca gaagactcta attattcttc gctcgactgg gaaaactgtt
     7861 aacaactgct cgagtgtggt cggagcggct cacagtgcgt tttcccgtag acaaaaatca
     7921 aatttcatgg tatttttcgg cgataaacag taagttaaag ggaacccgaa tttagtgcta
     7981 tataatttaa ttccactaga gtttgtatcc tttgatagat acgcgtattt cgacctcaac
     8041 tgcaaggccg tcgtgtacta gacttgacta atccagactg gtcttttagt tatgacttct
     8101 gtccacatct ccatacattc aacgcactgt gcggctgtgc tgtgcgactc cgtcgagtcg
     8161 accaacatag ttgaaacaaa ttgaatattt aattgatcgt tataggaatg gtgttagatg
     8221 agtcatcctt tacagtaagc acatacagta ttataattga agatcgtcgg cagataggtg
     8281 tgtagggtag agtatcagca ataagttggg acgtttgact ttttgtaggt agacaaaaac
     8341 taaacttttt ttcgcttctc tatgtgtgcc cctgggtagc gttccgttcc gattggggtg
     8401 cgaacgaatg aaatcgccca tcgagttgat acgtccatcc atcgctagaa ccgcgttcgc
     8461 tgtagaagac tatataagag cagaggcaag agtagtgaaa tgataggagc caagttctgg
     8521 ggtcttagag ctatgctgga aacagcatag caagttaaga taaggctagt ccgttatcaa
     8581 cttgaaaaag tggcaccgag tcggtgcttt ttttggtggt tgttcatcga ttcctaatat
     8641 tgcagtgaag cagtttgttg tgcatggcga agataaggtt agggcgagga atgccttgga
     8701 aataagcggc actgggccgt atgaataaaa agcgttgaac tttactacat gtagcatcta
     8761 ctcaaaaaca aaatccacaa agtttactac acttttggta tgaataaaaa ccttttcgaa
     8821 catgtagtac ctactacttt aagaaaattc tattcaaagt acagagtaat gctgcatgtt
     8881 aagaaaattc cattcagagt gactcttgaa atcaataaaa tcatgcatat atgccaaatt
     8941 tgtcacttat tcacgtgaca aattatgtga aaatatgaat attactacat tcccgctatg
     9001 catcgcgtaa tcgatcatga aatttatgtt ggttatgtaa ctttacagta ttccctttat
     9061 gtgcatgtga attttataat gatttgacaa caaggatttt ccttagaatt ttcgaatttt
     9121 agctagaaat ctgtatccta tgaatgaatc ttgagataac agcaattgta acattataaa
     9181 gttcctaaca ttttcaagat tccagtattg agtcgcgatc gcttaattaa ccgcggatat
     9241 gtcttgccga agcacattaa cccaagccat ttatatatca agtacaagtt gatttgattc
     9301 tagtgatgga ctgacacgaa tctatggcac tgttgcagag cttacaaaca ggtacccgga
     9361 gtcgcttcaa ttattcaatc atccattcaa aaacttccat ctcttccaat tagccaatat
     9421 tcaaattgga atccagccag ccgacggagg cagatggtct acctggcaac tatagagttt
     9481 ccatgtttcc agactttccc tcccggtaaa cggagacaaa acgacagacg taagtaggta
     9541 catatgcata ccgcacggac aaatcaaatt tgtctggcag ctccaattag agtcgttaaa
     9601 aatttaacga tgcgctaaat aacttcaagc tatttgtctc gctggattgg cttcgagtgg
     9661 taagatccta tcaaatgccg aaaacaaaaa actttcttct taattgttcg ttcttcaaca
     9721 cctctccatg gtgataacgg atacggtttc attgtcagca tccatcctcc gaaaaataca
     9781 ttacgccttg aaatatgcaa tcgcaaacac ggatctgttt ggaacattta ttttactatg
     9841 aagagatgcg ataggtaata tttatttgag cgtttaagat actcattgtt ctctcaaaga
     9901 atgtcattga aagccaacga ggtcaaatca aatattataa taaaaaggtc aaagaggact
     9961 aacttaaagc tctctttatg gataggaaaa aatattttcg cccatcgcta gaacttttac
    10021 cgtttccatt gagtatataa ctaagatgaa tgaggctaat tgatgtctcc gcaatacgcc
    10081 ccgggtctta gagctatgct ggaaacagca tagcaagtta agataaggct agtccgttat
    10141 caacttgaaa aagtggcacc gagtcggtgc tttttttaag atgcttcggc aattaataat
    10201 aaggaatggt aaagcattct tcaaatgtta ctatgttctc aaattttcaa gaattaggta
    10261 ttcaagaaat tctttcaggc atattccata atctgcacaa gcagctactt tagaaattat
    10321 tgccgggctt aaattatatg tacaacattt cctgagtatt ttctgacaaa aattctcaca
    10381 agggaaggtc acgatggagt tacacggggt ttttaaccgg actatttttg ttagattcta
    10441 catgtcgaaa tatgaaaaac cccaaagcct ttcgggtgat ggaccagtgg tgtagccagg
    10501 aggggctctg aaaggggtaa ttttgtacgt atattatatt attaagctaa ttggaaattt
    10561 gacaaaaata tttttttagt atttattgtg atggtagaga acaaaattga cattaatgta
    10621 cgtttgaaat gtatttgcca tgcctttggc gtactaacat ggagtatgga caccaaaacc
    10681 gatttcgcta gcgtttaaac ggcgcgccaa ttcgagctcg cccggggatc taattcaatt
    10741 agagactaat tcaattagag ctaattcaat taggatccaa gcttatcgat ttcgaaccct
    10801 cgaccgccgg agtataaata gaggcgcttc gtctacggag cgacaattca attcaaacaa
    10861 gcaaagtgaa cacgtcgcta agcgaaagct aagcaaataa acaagcgcag ctgaacaagc
    10921 taaacaatcg gggtaccgct agagtcgacg gtacgatcca ccggtcgcca ccatggtgag
    10981 caagggcgag gaggtcatca aagagttcat gcgcttcaag gtgcgcatgg agggctccat
    11041 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
    11101 gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc
    11161 cccccagttc atgtacggct ccaaggcgta cgtgaagcac cccgccgaca tccccgatta
    11221 caagaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
    11281 cggtctggtg accgtgaccc aggactcctc cctgcaggac ggcacgctga tctacaaggt
    11341 gaagatgcgc ggcaccaact tcccccccga cggccccgta atgcagaaga agaccatggg
    11401 ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca
    11461 ccaggccctg aagctgaagg acggcggcca ctacctggtg gagttcaaga ccatctacat
    11521 ggccaagaag cccgtgcaac tgcccggcta ctactacgtg gacaccaagc tggacatcac
    11581 ctcccacaac gaggactaca ccatcgtgga acagtacgag cgctccgagg gccgccacca
    11641 cctgttcctg gggcatggca ccggcagcac cggcagcggc agctccggca ccgcctcctc
    11701 cgaggacaac aacatggccg ttatcaagga atttatgcgc ttcaaagtta ggatggaggg
    11761 atccatgaac ggacatgagt tcgagatcga gggagagggc gagggacgcc cgtatgaagg
    11821 cacacaaaca gccaaactca aggtcaccaa gggcggacca ctgcccttcg cctgggatat
    11881 cctgagtccc cagtttatgt acggcagcaa ggcctacgtt aagcaccccg ctgacatacc
    11941 ggactacaaa aagctgtcct ttccggaagg cttcaagtgg gagcgcgtga tgaatttcga
    12001 agacggagga ctggtcactg tgacccaaga tagcagtttg caggacggta cactgatcta
    12061 taaggttaaa atgcgcggca ctaactttcc gccagatggc ccagtgatgc agaagaagac
    12121 catgggttgg gaggcatcca ccgaacgtct gtaccctcga gacggagtgc tcaagggcga
    12181 gatccatcag gccctcaaac tgaaagatgg tggtcactac ctggtcgaat ttaagaccat
    12241 ttacatggcc aagaagccgg ttcagctgcc cggatattat tatgtggata cgaaactgga
    12301 tataacttcg cataacgaag actacaccat tgtcgagcag tatgagcgca gcgaaggccg
    12361 acatcacctg ttcctctacg gcatggacga gctgtacaag taggcggccg cgactctaga
    12421 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
    12481 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
    12541 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
    12601 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaaagc ttgctagctc
    12661 atcacaaacg aaactattat aatcgtcttc aggggaacgg tagcatcata tctgtcacca
    12721 gtcagtcgga ttcctttctt tcggcataaa ttatgctcgg gtgacgagac gagttgaaaa
    12781 tcccgtaaat taaaacatat accacactcg ccgtcctaga cgacgacgac gacgacgatt
    12841 cccaaggaat tgaacccttc atttgcattt cactaccgct ctgagagatg caaagcgcgc
    12901 cagacagtcg atcgaatctg cagaccagag agtcacatca agaatgttga cattccacca
    12961 gaagactcta attattcttc gctcgactgg gaaaactgtt aacaactgct cgagtgtggt
    13021 cggagcggct cacagtgcgt tttcccgtag acaaaaatca aatttcatgg tatttttcgg
    13081 cgataaacag taagttaaag ggaacccgaa tttagtgcta tataatttaa ttccactaga
    13141 gtttgtatcc tttgatagat acgcgtattt cgacctcaac tgcaaggccg tcgtgtacta
    13201 gacttgacta atccagactg gtcttttagt tatgacttct gtccacatct ccatacattc
    13261 aacgcactgt gcggctgtgc tgtgcgactc cgtcgagtcg accaacatag ttgaaacaaa
    13321 ttgaatattt aattgatcgt tataggaatg gtgttagatg agtcatcctt tacagtaagc
    13381 acatacagta ttataattga agatcgtcgg cagataggtg tgtagggtag agtatcagca
    13441 ataagttggg acgtttgact ttttgtaggt agacaaaaac taaacttttt ttcgcttctc
    13501 tatgtgtgcc cctgggtagc gttccgttcc gattggggtg cgaacgaatg aaatcgccca
    13561 tcgagttgat acgtccatcc atcgctagaa ccgcgttcgc tgtagaagac tatataagag
    13621 cagaggcaag agtagtgaaa tctcacaagt agagctacac ggtcttagag ctatgctgga
    13681 aacagcatag caagttaaga taaggctagt ccgttatcaa cttgaaaaag tggcaccgag
    13741 tcggtgcttt ttttggtggt tgttcatcga ttcctaatat tgcagtgaag cagtttgttg
    13801 tgcatggcga agataaggtt agggcgagga atgccttgga aataagcggc actgggccgt
    13861 atgaataaaa agcgttgaac tttactacat gtagcatcta ctcaaaaaca aaatccacaa
    13921 agtttactac acttttggta tgaataaaaa ccttttcgaa catgtagtac ctactacttt
    13981 aagaaaattc tattcaaagt acagagtaat gctgcatgtt aagaaaattc cattcagagt
    14041 gactcttgaa atcaataaaa tcatgcatat atgccaaatt tgtcacttat tcacgtgaca
    14101 aattatgtga aaatatgaat attactacat tcccgctatg catcgcgtaa tcgatcatga
    14161 aatttatgtt ggttatgtaa ctttacagta ttccctttat gtgcatgtga attttataat
    14221 gatttgacaa caaggatttt ccttagaatt ttcgaatttt agctagaaat ctgtatccta
    14281 tgaatgaatc ttgagataac agcaattgta acattataaa gttcctaaca ttttcaagat
    14341 tccagtattg agtcgcgatc gcttaattaa ccgcggatat gtcttgccga agcacattaa
    14401 cccaagccat ttatatatca agtacaagtt gatttgattc tagtgatgga ctgacacgaa
    14461 tctatggcac tgttgcagag cttacaaaca ggtacccgga gtcgcttcaa ttattcaatc
    14521 atccattcaa aaacttccat ctcttccaat tagccaatat tcaaattgga atccagccag
    14581 ccgacggagg cagatggtct acctggcaac tatagagttt ccatgtttcc agactttccc
    14641 tcccggtaaa cggagacaaa acgacagacg taagtaggta catatgcata ccgcacggac
    14701 aaatcaaatt tgtctggcag ctccaattag agtcgttaaa aatttaacga tgcgctaaat
    14761 aacttcaagc tatttgtctc gctggattgg cttcgagtgg taagatccta tcaaatgccg
    14821 aaaacaaaaa actttcttct taattgttcg ttcttcaaca cctctccatg gtgataacgg
    14881 atacggtttc attgtcagca tccatcctcc gaaaaataca ttacgccttg aaatatgcaa
    14941 tcgcaaacac ggatctgttt ggaacattta ttttactatg aagagatgcg ataggtaata
    15001 tttatttgag cgtttaagat actcattgtt ctctcaaaga atgtcattga aagccaacga
    15061 ggtcaaatca aatattataa taaaaaggtc aaagaggact aacttaaagc tctctttatg
    15121 gataggaaaa aatattttcg cccatcgcta gaacttttac cgtttccatt gagtatataa
    15181 ctaagatgaa tgaggctaat tgatctgtcg tcgttttttt ccgggtctta gagctatgct
    15241 ggaaacagca tagcaagtta agataaggct agtccgttat caacttgaaa aagtggcacc
    15301 gagtcggtgc tttttttaag atgcttcggc aattaataat aaggaatggt aaagcattct
    15361 tcaaatgtta ctatgttctc aaattttcaa gaattaggta ttcaagaaat tctttcaggc
    15421 atattccata atctgcacaa gcagctactt tagaaattat tgccgggctt aaattatatg
    15481 tacaacattt cctgagtatt ttctgacaaa aattctcaca agggaaggtc acgatggagt
    15541 tacacggggt ttttaaccgg actatttttg ttagattcta catgtcgaaa tatgaaaaac
    15601 cccaaagcct ttcgggtgat ggaccagtgg tgtagccagg aggggctctg aaaggggtaa
    15661 ttttgtacgt atattatatt attaagctaa ttggaaattt gacaaaaata tttttttagt
    15721 atttattgtg atggtagaga acaaaattga cattaatgta cgtttgaaat gtatttgcca
    15781 tgcctttggc gtactaacat ggagtatgga caccaaaacc gatttcgttt aaacgatctc
    15841 ggatctgaca atgttcagtg cagagactcg gctacgcctc gtggactttg aagttgacca
    15901 acaatgttta ttcttacctc taatagtcct ctgtggcaag gtcaagattc tgttagaagc
    15961 caatgaagaa cctggttgtt caataacatt ttgttcgtct aatatttcac taccgcttga
    16021 cgttggctgc acttcatgta cctcatctat aaacgcttct tctgtatcgc tctggacgtc
    16081 atcttcactt acgtgatctg atatttcact gtcagaatcc tcaccaacaa gctcgtcatc
    16141 gctttgcaga agagcagaga ggatatgctc atcgtctaaa gaactaccca ttttattata
    16201 tattagtcac gatatctata acaagaaaat atatatataa taagttatca cgtaagtaga
    16261 acatgaaata acaatataat tatcgtatga gttaaatctt aaaagtcacg taaaagataa
    16321 tcatgcgtca ttttgactca cgcggtcgtt atagttcaaa atcagtgaca cttaccgcat
    16381 tgacaagcac gcctcacggg agctccaagc ggcgactgag atgtcctaaa tgcacagcga
    16441 cggattcgcg ctatttagaa agagagagca atatttcaag aatgcatgcg tcaattttac
    16501 gcagactatc tttctagggt taaaaaagat ttgcgcttta ctcgacctaa actttaaaca
    16561 cgtcatagaa tcttcgtttg acaaaaacca cattgtggcc aagctgtgtg acgcgacgcg
    16621 cgctaaagaa tggcaaacca agtcgcgcga gcgtcgactc tagaggatcc ccgggtaccg
    16681 agctcgaatt cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
    16741 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
    16801 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
    16861 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
    16921 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
    16981 tcagctcact caaaggcggt aatacggt
//
