LOCUS       Exported               10620 bp ds-DNA     circular SYN 24-MAR-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10620)
  AUTHORS   AMOLF
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Mar 24, 2023 from SnapGene Viewer 4.3.10
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10620
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    176..197
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        212..242
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    250..266
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     274..290
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        311..329
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        357..583
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             584..764
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    811..936
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1429..1662
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1847..1891
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     2064..2084
                     /label=pLKO.FOR
     promoter        2180..2274
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     protein_bind    2228..2246
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2256..2274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(2257..2281)
                     /label=V_pLKO puro.REV
     primer_bind     2262..2300
                     /label=F_pLKO HA peptide.FOR
     misc_feature    2282..3101
                     /label=gblock
     misc_feature    2282..2292
                     /label=gblock1
     misc_feature    2282..2286
                     /label=MluI
     misc_feature    2293..2340
                     /label=HA - signal peptide
     misc_feature    2341..2367
                     /label=HA tag
     primer_bind     complement(2349..2371)
                     /label=V-pHR Hatag.REV2
     variation       2368..2913
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
     primer_bind     complement(2561..2578)
                     /label=pHR SNAP rev
     variation       2914..2925
                     /label=Linker
     misc_feature    2929..3081
                     /label=CD86Stalk&TM
     primer_bind     complement(3061..3081)
                     /label=V_pHR CD86eGFP.REV
     primer_bind     3062..3099
                     /label=F_eGFP.FOR
     primer_bind     complement(3080..3121)
                     /label=V_CD86.REV
     CDS             3082..3798
                     /codon_start=1
                     /label=eGFP
                     /note="/vntifkey=4"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     variation       3082..3101
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
     primer_bind     3096..3114
                     /label=F_eGFP-2.FOR
     primer_bind     complement(3231..3243)
                     /label=Q5SDM_R
     primer_bind     complement(3450..3469)
                     /label=eGFP.REV
     primer_bind     3759..3803
                     /label=V-FKBP.FOR
     primer_bind     complement(3766..3786)
                     /label=F_eGFP-2.REV
     primer_bind     complement(3778..3818)
                     /label=F_eGFP.REV
     misc_feature    3779..4137
                     /label=gblock
     variation       3779..3798
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
     primer_bind     3801..3820
                     /label=V-HaloFKBP.FOR
     primer_bind     3801..3820
                     /label=V_HaloFKBP.FOR
     variation       3811..4131
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
     primer_bind     complement(3888..3905)
                     /label=Q5SDM_R
     primer_bind     complement(3892..3924)
                     /label=f36v4.Rev
     primer_bind     3906..3928
                     /label=Q5SDM_F
     primer_bind     complement(4110..4157)
                     /label=F_pLKO puro FKBP.REV
     misc_feature    4136..4137
                     /label=Not
     primer_bind     4139..4163
                     /label=V-pLKO.FOR
     misc_feature    4179..4296
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     complement(4321..4338)
                     /label=pLKO.REV
     promoter        4345..4855
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     intron          4864..5436
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     promoter        5491..5509
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5520..6137
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESG"
     misc_feature    6175..6748
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             6768..7367
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     LTR             7495..7728
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7800..7921
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      7961..8096
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(8117..8135)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8145..8161)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      8303..8758
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        8784..8888
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8889..9749
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      9920..10508
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
       61 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
      121 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
      181 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
      241 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
      301 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta
      361 gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt
      421 acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc
      481 cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca
      541 ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga
      601 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
      661 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
      721 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
      781 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
      841 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
      901 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
      961 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     1021 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     1081 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     1141 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     1201 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     1261 accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg
     1321 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
     1381 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
     1441 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
     1501 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
     1561 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
     1621 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
     1681 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
     1741 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     1801 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     1861 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     1921 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     1981 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
     2041 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
     2101 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc gatcacgaga
     2161 ctagcctcga gcggccgcaa tatttgcatg tcgctatgtg ttctgggaaa tcaccataaa
     2221 cgtgaaatcc ctatcagtga tagagactta taagttccct atcagtgata gagacaccgg
     2281 tcgcgtgcca ccatgaagac catcatcgcc ctgagctaca tcttctgcct ggtgttcgcc
     2341 tacccatacg atgttccaga ttacgctatg gacaaagatt gcgaaatgaa gcgcaccacc
     2401 ctggatagcc ctctgggcaa actggaactg tctgggtgcg aacagggcct gcaccgtatc
     2461 atcttcctgg gcaaaggaac atctgcagct gacgccgttg aagtgcctgc tccagccgca
     2521 gtgctgggcg gaccagagcc actgatgcag gccaccgcct ggctcaacgc ctactttcac
     2581 cagcctgagg ccatcgagga gttccctgtg ccagccctgc accacccagt gttccagcag
     2641 gagagcttta cccgccaggt gctgtggaaa ctgctgaaag tggtgaagtt cggagaggtc
     2701 atcagctaca gccacctggc tgccctggca ggcaatcccg cagccacagc tgcagtgaaa
     2761 acagctctga gcggaaatcc cgtgcccatt ctgatcccct gccaccgggt tgtacaggga
     2821 gacctggatg ttggaggtta cgagggcgga ctcgccgtga aagagtggct gctggcccac
     2881 gagggccaca gactgggcaa gcctgggctg ggtggtagtg gtggatcccg gcttttatct
     2941 tcacctttct ctatagagct tgaggaccct cagcctcccc cagaccacat tccttggatt
     3001 acagctgtac ttccaacagt tattatatgt gtgatggttt tctgtctaat tctatggaaa
     3061 tggaagaaaa agaagcggcc tatggtgagc aagggcgagg agctgttcac cggggtggtg
     3121 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     3181 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     3241 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     3301 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     3361 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     3421 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     3481 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     3541 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     3601 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     3661 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     3721 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     3781 atggacgagc tgtacaagac tagtggatcc ggtgtgcagg tcgagacaat ttctcctggc
     3841 gacggccgga cattccctaa acgaggacag acatgcgtgg tgcactacac cggcatgctg
     3901 gaagatggca agaagttcga cagcagccgg gatagaaaca aacccttcaa gttcatgctg
     3961 ggcaagcaag aagtgatcag aggttgggaa gagggcgtcg ctcagatgtc tgttggacag
     4021 agagccaagc tgacaatcag ccccgattac gcctatggcg ccacaggaca ccctggcatc
     4081 attcctccac atgctacact ggtgttcgac gtggaactgc tgaagctgga ataaagcgaa
     4141 ttctcgacct cgagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
     4201 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     4261 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
     4321 gatccacttt ggccgcggct cgagggggtt ggggttgcgc cttttccaag gcagccctgg
     4381 gtttgcgcag ggacgcggct gctctgggcg tggttccggg aaacgcagcg gcgccgaccc
     4441 tgggtctcgc acattcttca cgtccgttcg cagcgtcacc cggatcttcg ccgctaccct
     4501 tgtgggcccc ccggcgacgc ttcctgctcc gcccctaagt cgggaaggtt ccttgcggtt
     4561 cgcggcgtgc cggacgtgac aaacggaagc cgcacgtctc actagtaccc tcgcagacgg
     4621 acagcgccag ggagcaatgg cagcgcgccg accgcgatgg gctgtggcca atagcggctg
     4681 ctcagcaggg cgcgccgaga gcagcggccg ggaaggggcg gtgcgggagg cggggtgtgg
     4741 ggcggtagtg tgggccctgt tcctgcccgc gcggtgttcc gcattctgca agcctccgga
     4801 gcgcacgtcg gcagtcggct ccctcgttga ccgaatcacc gacctctctc cccaggggga
     4861 tctgtgagtt tggggaccct tgattgttct ttctttttcg ctattgtaaa attcatgtta
     4921 tatggagggg gcaaagtttt cagggtgttg tttagaatgg gaagatgtcc cttgtatcac
     4981 catggaccct catgataatt ttgtttcttt cactttctac tctgttgaca accattgtct
     5041 cctcttattt tcttttcatt ttctgtaact ttttcgttaa actttagctt gcatttgtaa
     5101 cgaattttta aattcacttt tgtttatttg tcagattgta agtactttct ctaatcactt
     5161 ttttttcaag gcaatcaggg tatattatat tgtacttcag cacagtttta gagaacaatt
     5221 gttataatta aatgataagg tagaatattt ctgcatataa attctggctg gcgtggaaat
     5281 attcttattg gtagaaacaa ctacatcctg gtcatcatcc tgcctttctc tttatggtta
     5341 caatgatata cactgtttga gatgaggata aaatactctg agtccaaacc gggcccctct
     5401 gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt
     5461 gtgctgtctc atcattttgg caaagaattg taatacgact cactataggg cgaattgata
     5521 tgtctagatt agataaaagt aaagtgatta acagcgcatt agagctgctt aatgaggtcg
     5581 gaatcgaagg tttaacaacc cgtaaactcg cccagaagct aggtgtagag cagcctacat
     5641 tgtattggca tgtaaaaaat aagcgggctt tgctcgacgc cttagccatt gagatgttag
     5701 ataggcacca tactcacttt tgccctttag aaggggaaag ctggcaagat tttttacgta
     5761 ataacgctaa aagttttaga tgtgctttac taagtcatcg cgatggagca aaagtacatt
     5821 taggtacacg gcctacagaa aaacagtatg aaactctcga aaatcaatta gcctttttat
     5881 gccaacaagg tttttcacta gagaatgcat tatatgcact cagcgctgtg gggcatttta
     5941 ctttaggttg cgtattggaa gatcaagagc atcaagtcgc taaagaagaa agggaaacac
     6001 ctactactga tagtatgccg ccattattac gacaagctat cgaattattt gatcaccaag
     6061 gtgcagagcc agccttctta ttcggccttg aattgatcat atgcggatta gaaaaacaac
     6121 ttaaatgtga aagtgggtcc gcgtacagcg gctcccggga gttctaggga tctgcccctc
     6181 tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt
     6241 tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc
     6301 tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca
     6361 aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac
     6421 gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg
     6481 ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt
     6541 gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct
     6601 gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg
     6661 ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt
     6721 ggttttcctt tgaaaaacac gatgataagg atccaccgga gcttaccatg accgagtaca
     6781 agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg
     6841 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc
     6901 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
     6961 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
     7021 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
     7081 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
     7141 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
     7201 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
     7261 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
     7321 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc ccgccccacg
     7381 acccgcagcg cccgaccgaa aggagcgcac gaccccatgc atcggtacct ttaagaccaa
     7441 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     7501 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     7561 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     7621 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     7681 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat
     7741 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga
     7801 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     7861 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     7921 atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc
     7981 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     8041 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     8101 gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
     8161 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     8221 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     8281 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     8341 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     8401 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     8461 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     8521 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     8581 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     8641 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     8701 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
     8761 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     8821 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     8881 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     8941 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     9001 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     9061 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     9121 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     9181 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     9241 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     9301 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     9361 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     9421 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     9481 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     9541 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     9601 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     9661 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     9721 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     9781 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     9841 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     9901 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     9961 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
    10021 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
    10081 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
    10141 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    10201 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    10261 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    10321 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    10381 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    10441 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    10501 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
    10561 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
//
