LOCUS       pSAH69____OLQp__        4646 bp    DNA        circular     22-MAR-2023
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     Alharbi & Froekjaer-Jensen, unpublished reagents. For details, see
            www.wormbuilder.org/BioPart
COMMENT     Red fluorescence marker and cloning vector for expression in the
            OLQ neurons. 
COMMENT     The vector is compatible with single-copy insertions using MosTI
            (El Mouridi et al., 2022) but can also be used in extra-chromosomal
            arrays. 
COMMENT     Verified expression in OLQ in hermaphrodites and males in
            extrachromosomal arrays and visible expression on fluorescence
            dissection microscope from single-copy insertions. 
COMMENT     Use BsaI to exchange the fluorophore for a different transgene
            (AAAA and GAGC Golden-gate overhangs).
COMMENT     Use ApaI for N-terminal fusions
COMMENT     Use SmaI for C-terminal fusions
COMMENT     Use EcoRV to linearize the construct and remove MosTI sequences.
COMMENT     Sequence annotations are optimized for use with ApE: A Plasmid
            Editor (https://doi.org/10.3389/fbinf.2022.818619)
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    7..121
                     /locus_tag="Decoy sequence (start, intron, stop)"
                     /label="Decoy sequence (start, intron, stop)"
                     /ApEinfo_label="Decoy sequence (start, intron, stop)"
                     /ApEinfo_fwdcolor="#ff84ff"
                     /ApEinfo_revcolor="#ff84ff"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    126..421
                     /locus_tag="OLQp (WBGene00003841, ocr-4)"
                     /label="OLQp (WBGene00003841, ocr-4)"
                     /ApEinfo_label="OLQp (WBGene00003841, ocr-4)"
                     /ApEinfo_fwdcolor="#f8d471"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    427..432
                     /locus_tag="BsaI - exchange mScarlet"
                     /label="BsaI - exchange mScarlet"
                     /ApEinfo_label="BsaI - exchange mScarlet"
                     /ApEinfo_fwdcolor="#fe9200"
                     /ApEinfo_revcolor="#00fa92"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    join(7..26,78..121)
                     /locus_tag="Fire Decoy (start, intron, stop)"
                     /label="Fire Decoy (start, intron, stop)"
                     /ApEinfo_label="Fire Decoy (start, intron, stop)"
                     /ApEinfo_fwdcolor="#ff84ff"
                     /ApEinfo_revcolor="#ff84ff"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             join(437..439,446..580,656..848,902..1059,1117..1323,
                     1330..1332)
                     /locus_tag="wrmScarlet(rps-20)"
                     /label="wrmScarlet(rps-20)"
                     /ApEinfo_label="wrmScarlet(rps-20)"
                     /ApEinfo_fwdcolor="#f5b5b1"
                     /ApEinfo_revcolor="#f5b5b1"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             complement(2894..3754)
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#0080ff"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1333..1339
                     /locus_tag="BsaI - exchange wrmScarlet"
                     /label="BsaI - exchange wrmScarlet"
                     /ApEinfo_label="BsaI - exchange wrmScarlet"
                     /ApEinfo_fwdcolor="#fe9200"
                     /ApEinfo_revcolor="#00fa92"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     3'UTR           1346..1514
                     /locus_tag="tbb-2 3'UTR"
                     /label="tbb-2 3'UTR"
                     /ApEinfo_label="tbb-2 3'UTR"
                     /ApEinfo_fwdcolor="#d4fb78"
                     /ApEinfo_revcolor="#d4fb78"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1521..1820
                     /locus_tag="Right repair homology"
                     /label="Right repair homology"
                     /ApEinfo_label="Right repair homology"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             4393..4499
                     /locus_tag="cbr-unc-119 (Exons 3-4)"
                     /label="cbr-unc-119 (Exons 3-4)"
                     /ApEinfo_label="cbr-unc-119 (Exons 3-4)"
                     /ApEinfo_fwdcolor="#8000ff"
                     /ApEinfo_revcolor="#8000ff"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1821..1841
                     /locus_tag="Uni_9 R"
                     /label="Uni_9 R"
                     /ApEinfo_label="Uni_9 R"
                     /ApEinfo_fwdcolor="#cc66ff"
                     /ApEinfo_revcolor="#cc66ff"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(1877..1893)
                     /locus_tag="M13 rev"
                     /label="M13 rev"
                     /ApEinfo_label="M13 rev"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     rep_origin      complement(2065..2653)
                     /locus_tag="ori"
                     /label="ori"
                     /ApEinfo_label="ori"
                     /ApEinfo_fwdcolor="#b4abac"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     promoter        complement(3755..3859)
                     /locus_tag="AmpR promoter"
                     /label="AmpR promoter"
                     /ApEinfo_label="AmpR promoter"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     3'UTR           4500..4612
                     /locus_tag="eft-3 3'UTR"
                     /label="eft-3 3'UTR"
                     /ApEinfo_label="eft-3 3'UTR"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     3973..3989
                     /locus_tag="M13 fwd"
                     /label="M13 fwd"
                     /ApEinfo_label="M13 fwd"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    4020..4041
                     /locus_tag="Uni_9 F"
                     /label="Uni_9 F"
                     /ApEinfo_label="Uni_9 F"
                     /ApEinfo_fwdcolor="#cc66ff"
                     /ApEinfo_revcolor="#cc66ff"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             4042..4341
                     /locus_tag="cbr-unc-119 (Exons 3-4)(1)"
                     /label="cbr-unc-119 (Exons 3-4)(1)"
                     /ApEinfo_label="cbr-unc-119 (Exons 3-4)"
                     /ApEinfo_fwdcolor="#8000ff"
                     /ApEinfo_revcolor="#8000ff"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_recomb     4613..4646
                     /locus_tag="LoxP"
                     /label="LoxP"
                     /ApEinfo_label="LoxP"
                     /ApEinfo_fwdcolor="#d59687"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1..6
                     /locus_tag="EcoRV - linearize"
                     /label="EcoRV - linearize"
                     /ApEinfo_label="EcoRV - linearize"
                     /ApEinfo_fwdcolor="#fa9225"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    440..445
                     /locus_tag="Apal - N-terminal fusion"
                     /label="Apal - N-terminal fusion"
                     /ApEinfo_label="Apal - N-terminal fusion"
                     /ApEinfo_fwdcolor="#fe9200"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1324..1329
                     /locus_tag="Smal - C-terminal fusion"
                     /label="Smal - C-terminal fusion"
                     /ApEinfo_label="Smal - C-terminal fusion"
                     /ApEinfo_fwdcolor="#fe9200"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1515..1520
                     /locus_tag="EcoRV - linearize(1)"
                     /label="EcoRV - linearize(1)"
                     /ApEinfo_label="EcoRV - linearize"
                     /ApEinfo_fwdcolor="#fc9202"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 gatatcaaaa ATGATTACGC CAAGCTgtaa gtttaaacat gatcttacta actaactatt
       61 ctcatttaaa ttttcagAGC TTAAAAATGG CTGAAATCAC TCACAACGAT GGATACGCTA
      121 Atgcctcatt atgctatttg ccgattttct ttcaggaagg tttacggttt gaataatggc
      181 acgaagatac atagatgtat ttataattta ccgatggctt gattgcattg taataaaccg
      241 tcaggaaaga aaaataacat tttcccagac cctatatgct acatattact cagtgaacca
      301 tagaaatgtc aaatttccgg ttatgaggca taagtgagaa taaaggtatg tgagcaccgt
      361 agtttcttat ttacaatttc ccatcgaaga aaaagtaaaa tattctctga atctaacttg
      421 taaaaagaga ccaaaaATGG GGCCCGTTTC AAAGGGAGAG GCCGTTATAA AAGAATTTAT
      481 GCGTTTCAAG GTCCACATGG AGGGATCCAT GAACGGACAC GAGTTCGAGA TCGAGGGAGA
      541 GGGAGAGGGA CGTCCATACG AGGGAACCCA AACCGCCAAG gtgagtttta ttatcgattt
      601 tcgggatttt ctacttgtaa ttaccttttg tttttaataa gttgcttttt tccagCTCAA
      661 GGTCACCAAG GGAGGACCAC TCCCATTCTC CTGGGACATC CTCTCCCCAC AATTCATGTA
      721 CGGATCCCGT GCCTTCACCA AGCACCCAGC CGACATCCCA GACTACTACA AGCAATCCTT
      781 CCCAGAGGGA TTCAAGTGGG AGCGTGTCAT GAACTTCGAG GACGGAGGAG CCGTCACCGT
      841 CACCCAAGgt aggttttcag tttcaatttt tgttgcaact acctcaactc tatgttttca
      901 gACACCTCCC TCGAGGACGG AACCCTCATC TACAAGGTCA AGCTCCGTGG AACCAACTTC
      961 CCACCAGACG GACCAGTCAT GCAAAAGAAG ACCATGGGAT GGGAGGCCTC CACCGAGCGA
     1021 CTCTACCCAG AGGACGGAGT CCTCAAGGGA GACATCAAGg taaatttttc aagcttctaa
     1081 attccaaaat aaaccatttt aatttcttaa ttccagATGG CCCTCCGGCT CAAGGACGGA
     1141 GGACGTTACC TCGCCGACTT CAAGACCACC TACAAGGCCA AGAAGCCAGT CCAAATGCCA
     1201 GGAGCCTACA ACGTCGACCG TAAGCTCGAC ATCACCTCCC ACAACGAGGA CTACACCGTC
     1261 GTCGAGCAAT ACGAGCGTTC CGAGGGACGT CACTCCACCG GAGGAATGGA CGAGCTCTAC
     1321 AAGCCCGGGT GAtggtctct gagctatgca aaatcctttc aagcattccc ttcttctcta
     1381 tcactcttct ttctttttgt caaaaaattc tctcgctaat ttatttgctt ttttaatgtt
     1441 attattttat gactttttat agtcactgaa aagtttgcat ctgagtgaag tgaatgctat
     1501 caaaatgtga ttctgatatc tcaggaaacg gagaatctgt gcggcaatgt cttatcattt
     1561 gaaacgcgcc gtatcgatgc tgacatcatg cggctcccgt gttagtcgag aagatgggta
     1621 cgggccttta ttcatccaac actctacggc ttcttcaaga gctagtaggg caccctgtag
     1681 ttggaaaggg attatttcgt atggcgagcc cataccgtct ttcttgcgga agcttaacac
     1741 gataggaagt tggatagttt cgaacgatgg ttccatacgg aacgctgtct caaggatgag
     1801 tctgacggag tgtaactcga aggctaggtg gaggctcagt gatgataagt ctgcgatggt
     1861 ggatgcatgt gtcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcagagggc
     1921 acaatcctat tccgcgctat ccgacaatct ccaagacatt aggtggagtt cagttcggcg
     1981 tatggcatat gtcgctggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     2041 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     2101 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     2161 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     2221 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     2281 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     2341 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     2401 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     2461 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     2521 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     2581 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     2641 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc tattcaacaa
     2701 agccgccgtc ccgtcaagtc agcgtaaatg ggtagggggc ttcaaatcgt cctcgtgata
     2761 ccaattcgga gcctgctttt ttgtacaaac ttgttgataa tggcaattca aggatcttca
     2821 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     2881 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     2941 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     3001 taccatctgg ccccagtgct gcaatgatac cgcgagagcc acgctcaccg gctccagatt
     3061 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     3121 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     3181 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     3241 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     3301 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     3361 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     3421 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     3481 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     3541 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     3601 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     3661 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     3721 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     3781 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     3841 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc agatacctga aacaaaaccc
     3901 atcgtacggc caaggaagtc tccaataact gtgatccacc acaagcgcca gggttttccc
     3961 agtcacgacg ttgtaaaacg acggccagtc atgcataatc cgcacgcatc tggaataagg
     4021 aagtgccatt ccgcctgacc tAAGTGCTTT TCGAGATCGC CAAACCGGAG AACGATCAGG
     4081 AGAATGATGA GTCGCCACAG GAGTCGGCAA GATACGTGCG TTATAGATTT GCTCCAAACT
     4141 TTTTGAAACT CAAAACGGTC GGAGCAACTG TGGAATTCAA AGTAGGAGAC ATCCCAATCC
     4201 ATCATTTCCG AATGATCGAA CGTCACTTCT TCAAAGATCG CCTTCTGAAG TGTTTTGACT
     4261 TTGAATTCGG ATTCTGTATT CCGAATTCAC GAAACAACTG TGAACATATC TATGAGTTCC
     4321 CTCAACTCTC TCAACAACTC Agtgagtcat tattctaaaa agtacaattc aaaagactaa
     4381 tctctttttc agTGGACGAC ATGATCAACA ATCCAAACGA GACTCGTTCT GACAGCTTCT
     4441 ATTTCGTCGA TAACAAACTC GTCATGCACA ACAAAGCCGA CTACTCATAT GATGCATAAa
     4501 tcttcattgt tgagtttatc ttgttgattt ttgaataaat tatcaactct ttacttttta
     4561 atgggttatg aaataaataa acattgaaaa ctgataaaca acgttcatct ctataacttc
     4621 gtatagcata cattatacga agttat   
//
