LOCUS       Exported                6210 bp DNA     circular SYN 03-NOV-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6210)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Mar 24, 2023 from SnapGene 5.3.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6210
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             1762..3225
                     /codon_start=1
                     /label=NiV-F ecto
                     /note="color: #993366"
                     /translation="MVVILDKRCYCNLLILILMISECSVGILHYEKLSKIGLVKGVTRK
                     YKIKSNPLTKDIVIKMIPNVSNMSQCTGSVMENYKTRLNGILTPIKGALEIYKNNTHDL
                     VGDVRLAGVIMAGVAIGIATAAQITAGVALYEAMKNADNINKLKSSIESTNEAVVKLQE
                     TAEKTVYVLTALQDYINTNLVPTIDKISCKQTELSLDLALSKYLSDLLFVFGPNLQDPV
                     SNSMTIQAISQAFGGNYETLLRTLGYATEDFDDLLESDSITGQIIYVDLSSYYIIVRVY
                     FPILTEIQQAYIQELLPVSFNNDNSEWISIVPNFILVRNTLISNIEIGFCLITKRSVIC
                     NQDYATPMTNNMRECLTGSTEKCPRELVVSSHVPRFALSNGVLFANCISVTCQCQTTGR
                     AISQSGEQTLLMIDNTTCPTAVLGNVIISLGKYLGSVNYNSEGIAIGPPVFTDKVDISS
                     QISSMNQSLQQSKDYIKEAQRLLDTVNPSL"
     misc_feature    3235..3315
                     /label=Foldon
                     /note="color: #ff80ff; direction: RIGHT"
     CDS             3322..3345
                     /label=PreScission site
                     /note="color: #008000; direction: RIGHT"
     CDS             3349..3366
                     /label=6His
                     /note="color: #008000; direction: RIGHT"
     CDS             3352..3369
                     /label=6His
                     /note="color: #008000; direction: RIGHT"
     CDS             3355..3372
                     /label=6His
                     /note="color: #008000; direction: RIGHT"
     CDS             3379..3402
                     /label=Strep tag II
                     /note="color: #008000; direction: RIGHT"
     CDS             3442..3465
                     /label=Strep tag II
                     /note="color: #008000; direction: RIGHT"
     primer_bind     complement(4042..4062)
                     /label=M13-rev
                     /note="color: #008b8b; direction: LEFT"
     misc_binding    complement(4068..4090)
                     /label=LacO
                     /note="color: #6495ed; direction: LEFT"
     promoter        complement(4095..4124)
                     /label=lac
                     /note="color: #4682b4; direction: LEFT"
     rep_origin      complement(4442..5070)
                     /direction=LEFT
                     /label=ColE1 origin
                     /note="color: #4682b4"
     CDS             complement(5222..5881)
                     /label=AmpR
                     /note="color: #8fbc8f; direction: LEFT"
     promoter        complement(6121..6149)
                     /label=Amp prom
                     /note="color: #4682b4; direction: LEFT"
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc
     1321 tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag
     1381 ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc
     1441 tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
     1501 cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg
     1561 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
     1621 ggcggctcta gcgcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
     1681 ggcaacgtgc tggttgttgt gctgtctcat cattttggca aagaattgcg gccgtctcag
     1741 gccgagttcg gtaccgccac catggtggtg atcctggaca agaggtgcta ctgcaacctg
     1801 ctgatcctga tcctgatgat cagcgagtgc agcgtgggca tcctgcacta cgagaagctg
     1861 agcaagatcg gcctggtgaa gggcgtgacc aggaagtaca agatcaagag caaccccctg
     1921 accaaggaca tcgtgatcaa gatgatcccc aacgtgagca acatgagcca gtgcaccggc
     1981 agcgtgatgg agaactacaa gaccaggctg aacggcatcc tgacccccat caagggcgcc
     2041 ctggagatct acaagaacaa cacccacgac ctggtgggcg acgtgaggct ggccggcgtg
     2101 atcatggccg gcgtggccat cggcatcgcc accgccgccc agatcaccgc cggcgtggcc
     2161 ctgtacgagg ccatgaagaa cgccgacaac atcaacaagc tgaagagcag catcgagagc
     2221 accaacgagg ccgtggtgaa gctgcaggag accgccgaga agaccgtgta cgtgctgacc
     2281 gccctgcagg actacatcaa caccaacctg gtgcccacca tcgacaagat cagctgcaag
     2341 cagaccgagc tgagcctgga cctggccctg agcaagtacc tgagcgacct gctgttcgtg
     2401 ttcggcccca acctgcagga ccccgtgagc aacagcatga ccatccaggc catcagccag
     2461 gccttcggcg gcaactacga gaccctgctg aggaccctgg gctacgccac cgaggacttc
     2521 gacgacctgc tggagagcga cagcatcacc ggccagatca tctacgtgga cctgagcagc
     2581 tactacatca tcgtgagggt gtacttcccc atcctgaccg agatccagca ggcctacatc
     2641 caggagctgc tgcccgtgag cttcaacaac gacaacagcg agtggatcag catcgtgccc
     2701 aacttcatcc tggtgaggaa caccctgatc agcaacatcg agatcggctt ctgcctgatc
     2761 accaagagga gcgtgatctg caaccaggac tacgccaccc ccatgaccaa caacatgagg
     2821 gagtgcctga ccggcagcac cgagaagtgc cccagggagc tggtggtgag cagccacgtg
     2881 cccaggttcg ccctgagcaa cggcgtgctg ttcgccaact gcatcagcgt gacctgccag
     2941 tgccagacca ccggcagggc catcagccag agcggcgagc agaccctgct gatgatcgac
     3001 aacaccacct gccccaccgc cgtgctgggc aacgtgatca tcagcctggg caagtacctg
     3061 ggcagcgtga actacaacag cgagggcatc gccatcggcc cccccgtgtt caccgacaag
     3121 gtggacatca gcagccagat cagcagcatg aaccagagcc tgcagcagag caaggactac
     3181 atcaaggagg cccagaggct gctggacacc gtgaacccca gcctgggatc cggatacatc
     3241 cccgaggccc ccagagatgg ccaggcctac gtgcggaagg acggcgagtg ggtactgctg
     3301 agcacattcc tgggcagatc cctggaggtg ctgttccagg gcccaggcca tcaccaccat
     3361 caccaccatc atagcgcctg gtcccacccc cagttcgaga agggcggcgg tagtggaggg
     3421 ggcggatctg gcggctcagc ttggagccac ccccagttcg aaaagtgata atgactcgag
     3481 cgataattca ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg tgtggccaat
     3541 gccctggctc acaaatacca ctgagatctt tttccctctg ccaaaaatta tggggacatc
     3601 atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata
     3661 gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta
     3721 aaacatcaga atgagtattt ggtttagagt ttggcaacat atgccatatg ctggctgcca
     3781 tgaacaaagg tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc
     3841 cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt
     3901 atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct
     3961 cctctcctga ctactcccag tcatagctgt ccctcttctc ttatgaagat ccctcgacct
     4021 gcagcccaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     4081 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     4141 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     4201 tgtcgtgcca gcggatcgat ccgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     4261 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     4321 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     4381 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     4441 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     4501 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     4561 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     4621 cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt
     4681 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     4741 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     4801 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     4861 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     4921 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     4981 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     5041 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     5101 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     5161 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     5221 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     5281 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     5341 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     5401 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     5461 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     5521 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     5581 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     5641 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     5701 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     5761 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     5821 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     5881 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     5941 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     6001 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     6061 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     6121 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     6181 cgcgcacatt tccccgaaaa gtgccacctg
//
