LOCUS       Exported                6195 bp DNA     circular SYN 22-AUG-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6195)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Mar 24, 2023 from SnapGene 5.3.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6195
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             1762..3210
                     /codon_start=1
                     /label=LayV-F Ectodomain
                     /note="color: #ffcc99"
                     /translation="MAFLKSAIICYLLFYPHIVKSSLHYDSLSKVGIIKGLTYNYKIKG
                     SPSTKLMVVKLIPNIDGVRNCTQKQFDEYKNLVKNVLEPVKLALNAMLDNVKSGNNKYR
                     FAGAIMAGVALGVATAATVTAGIALHRSNENAQAIANMKNAIQNTNEAVKQLQLANKQT
                     LAVIDTIRGEINNNIIPVINQLSCDTIGLSVGIKLTQYYSEILTAFGPALQNPVNTRIT
                     IQAISSVFNRNFDELLKIMGYTSGDLYEILHSGLIRGNIIDVDVEAGYIALEIEFPNLT
                     LVPNAVVQELMPISYNVDGDEWVTLVPRFVLTRTTLLSNIDTSRCTVTESSVICDNDYA
                     LPMSYELIGCLQGDTSKCAREKVVSSYVPRFALSDGLVYANCLNTICRCMDTDTPISQS
                     LGTTVSLLDNKKCLVYQVGDILISVGSYLGEGEYSADNVELGPPVVIDKIDIGNQLAGI
                     NQTLQNAEDYIEKSEEFLKGINPSI"
     misc_feature    3220..3300
                     /label=Foldon
                     /note="color: #ff80ff; direction: RIGHT"
     CDS             3307..3330
                     /label=PreScission site
                     /note="color: #008000; direction: RIGHT"
     CDS             3334..3351
                     /label=6His
                     /note="color: #008000; direction: RIGHT"
     CDS             3337..3354
                     /label=6His
                     /note="color: #008000; direction: RIGHT"
     CDS             3340..3357
                     /label=6His
                     /note="color: #008000; direction: RIGHT"
     CDS             3364..3387
                     /label=Strep tag II
                     /note="color: #008000; direction: RIGHT"
     CDS             3427..3450
                     /label=Strep tag II
                     /note="color: #008000; direction: RIGHT"
     primer_bind     complement(4027..4047)
                     /label=M13-rev
                     /note="color: #008b8b; direction: LEFT"
     misc_binding    complement(4053..4075)
                     /label=LacO
                     /note="color: #6495ed; direction: LEFT"
     promoter        complement(4080..4109)
                     /label=lac
                     /note="color: #4682b4; direction: LEFT"
     rep_origin      complement(4427..5055)
                     /direction=LEFT
                     /label=ColE1 origin
                     /note="color: #4682b4"
     CDS             complement(5207..5866)
                     /label=AmpR
                     /note="color: #8fbc8f; direction: LEFT"
     promoter        complement(6106..6134)
                     /label=Amp prom
                     /note="color: #4682b4; direction: LEFT"
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc
     1321 tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag
     1381 ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc
     1441 tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
     1501 cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg
     1561 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
     1621 ggcggctcta gcgcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
     1681 ggcaacgtgc tggttgttgt gctgtctcat cattttggca aagaattgcg gccgtctcag
     1741 gccgagttcg gtaccgccac catggccttt ctgaagtccg ccatcatctg ctacctgctg
     1801 ttctaccctc acatcgtgaa gtccagcctg cactacgaca gcctgagcaa agtgggcatc
     1861 atcaagggcc tgacctacaa ctacaagatc aagggcagcc ccagcaccaa gctgatggtg
     1921 gtcaagctga tccccaacat cgacggcgtg cggaactgta cccagaagca gttcgacgag
     1981 tacaagaacc tggtcaagaa cgtgctggaa cccgtgaagc tggccctgaa cgccatgctg
     2041 gacaatgtga agtccggcaa caacaagtac agattcgctg gcgccatcat ggccggcgtt
     2101 gcacttggag ttgccacagc cgctacagtg acagccggaa ttgccctgca cagaagcaac
     2161 gagaacgccc aggccattgc caacatgaag aacgccatcc agaacaccaa cgaggccgtg
     2221 aagcagctcc agctggccaa caaacagacc ctggccgtga tcgacaccat ccggggagag
     2281 atcaacaaca acatcatccc cgtgatcaac cagctgagct gtgacaccat cggcctgagc
     2341 gtgggaatca agctgaccca gtactacagc gagatcctga ccgccttcgg acccgctctg
     2401 cagaaccctg tgaacaccag aatcaccatc caggccatca gcagcgtgtt caaccggaac
     2461 ttcgacgagc tgctgaagat catgggctac accagcggcg acctgtatga gatcctgcac
     2521 agcggcctga tccggggcaa tatcatcgac gtggacgtgg aagccggcta tatcgccctg
     2581 gaaatcgagt tccccaacct gacactggtg cccaacgccg tggtgcaaga gctgatgccc
     2641 atctcctaca acgtggacgg cgacgagtgg gttaccctgg tgcctagatt cgtgctgacc
     2701 cggacaaccc tgctgagcaa catcgatacc agcagatgca ccgtgaccga gagcagcgtg
     2761 atctgcgaca acgattacgc cctgcctatg agctacgagc tgatcggatg tctgcagggc
     2821 gacaccagca agtgcgccag agaaaaggtg gtgtccagct acgtgcccag attcgccctg
     2881 tctgacggac tggtgtacgc caactgcctg aacaccatct gccggtgcat ggacaccgac
     2941 acacctatct ctcagagcct gggcaccacc gtgtctctgc tggataacaa gaaatgcctg
     3001 gtgtaccaag tgggcgacat cctgatctcc gtgggctctt atctcggcga gggcgagtac
     3061 agcgccgaca atgttgaact gggccctcct gtggtcatcg acaagatcga catcggcaat
     3121 cagctggccg gcatcaatca gaccctgcag aacgccgagg actacatcga gaagtctgaa
     3181 gagttcctga agggcatcaa ccccagcatc ggatccggat acatccccga ggcccccaga
     3241 gatggccagg cctacgtgcg gaaggacggc gagtgggtac tgctgagcac attcctgggc
     3301 agatccctgg aggtgctgtt ccagggccca ggccatcacc accatcacca ccatcatagc
     3361 gcctggtccc acccccagtt cgagaagggc ggcggtagtg gagggggcgg atctggcggc
     3421 tcagcttgga gccaccccca gttcgaaaag tgataatgac tcgagcgata attcactcct
     3481 caggtgcagg ctgcctatca gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa
     3541 taccactgag atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag
     3601 catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt
     3661 ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca tcagaatgag
     3721 tatttggttt agagtttggc aacatatgcc atatgctggc tgccatgaac aaaggtggct
     3781 ataaagaggt catcagtata tgaaacagcc ccctgctgtc cattccttat tccatagaaa
     3841 agccttgact tgaggttaga ttttttttat attttgtttt gtgttatttt tttctttaac
     3901 atccctaaaa ttttccttac atgttttact agccagattt ttcctcctct cctgactact
     3961 cccagtcata gctgtccctc ttctcttatg aagatccctc gacctgcagc ccaagcttgg
     4021 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     4081 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     4141 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagcgga
     4201 tcgatccgct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     4261 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     4321 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     4381 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     4441 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     4501 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     4561 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     4621 gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc
     4681 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     4741 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     4801 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     4861 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     4921 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     4981 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     5041 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     5101 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     5161 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     5221 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     5281 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     5341 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     5401 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     5461 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     5521 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     5581 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     5641 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     5701 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     5761 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     5821 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     5881 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     5941 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     6001 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     6061 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     6121 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     6181 gaaaagtgcc acctg
//
