LOCUS       firefly_luc_degronx66A_Esp3i_01              5905 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             complement(1490..3139)
                     /note="pLannotate"
                     /label="luciferase"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        complement(3234..4211)
                     /note="pLannotate"
                     /label="CMV IE94 promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             4875..5735
                     /note="pLannotate"
                     /label="AmpR"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      1..589
                     /note="pLannotate"
                     /label="ori"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     rep_origin      4289..4744
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     polyA_signal    complement(1195..1329)
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="addgene"
                     /identity="99.265"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA signal"
     CDS             complement(1370..1489)
                     /note="pLannotate"
                     /label="hPEST"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        4770..4874
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        913..943
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    complement(947..969)
                     /note="pLannotate"
                     /label="lac operator"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="95.83333333333334"
                     /fragment="False"
                     /other="protein bind"
     protein_bind    complement(877..898)
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein bind"
     promoter        complement(3181..3199)
                     /note="pLannotate"
                     /label="SP6 promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        1012..1030
                     /note="pLannotate"
                     /label="T3 promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             771..864
                     /note="pLannotate"
                     /label="lacI"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="8.679593721144968"
                     /fragment="True"
                     /other="CDS"
     CDS             4218..4287
                     /note="pLannotate"
                     /label="lacZ"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="2.3011176857330704"
                     /fragment="True"
                     /other="CDS"
     CDS             1140..1172
                     /note="pLannotate"
                     /label="large T antigen"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="1.5536723163841808"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgctccc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tgggtaccgg gcccaatgca ttggcgccgc ggccgcgaac
     1081 gtctcttttt tttttttttt tttttttttt tttttttttt tttttttttt tttttttttc
     1141 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     1201 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     1261 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     1321 gtctggatct acgtaatacg actcactata gttctagagg ctcgagttag acgttgatgc
     1381 gagctgaagc acaagcggct gggtgccggt ccataccgct ttcttgtgcg caggacatag
     1441 gcaatgtacc ggcggcttgc tcttcaacct caggcggaaa gccgtgagac acggcgatct
     1501 tgccgccctt cttggcctta atgagaatct cgcggatctt gcgggcgtcc aacttgccgg
     1561 tcagtccttt aggcacctcg tccacgaaca caacaccacc gcgcagcttc ttggcggttg
     1621 taacctggct ggccacatag tccacgatct ccttctcggt catggtttta ccgtgttcca
     1681 gcacgacgac tgcggcgggc agctcgccgg catcgtcgtc gggcaggccg gcgaccccgg
     1741 cgtcgaagat gttggggtgt tgcagcagga tgctctccag ttcggctggg gctacctggt
     1801 agcccttgta tttgatcagg ctcttcagcc ggtccacgat gaagaagtgc tcgtcctcgt
     1861 cccagtaggc gatgtcgccg ctgtgcagcc agccgtcctt gtcgatgaga gcgtttgtag
     1921 cctcggggtt gttaacgtag ccgctcatga tcatggggcc acggacgcac agctcgccgc
     1981 gctggttcac acccagtgtc ttaccggtgt ccaagtccac caccttagcc tcgaagaagg
     2041 gcaccacctt gcctactgcg ccaggcttgt cgtccccttc gggggtgatc agaatggcgc
     2101 tggttgtttc tgtcaggccg tagccctggc ggatgcctgg taggtggaag cgtttggcca
     2161 cggcctcacc tacctccttg ctgagcggcg ccccgccgct ggcgatctcg tgcaagttgc
     2221 ttaggtcgta cttgtcgatg agagtgctct tagcgaagaa gctaaatagt gtgggcacca
     2281 gcagggcaga ttgaatctta tagtcttgca agctgcgcaa gaatagctcc tcctcgaagc
     2341 ggtacatgag cacgacccga aagccgcaga tcaagtagcc cagcgtggtg aacatgccga
     2401 agccgtggtg aaatggcacc acgctgagga tagcggtgtc ggggatgatc tggttgccga
     2461 agatggggtc gcgggcatga ctgaatcgga cacaagcggt gcggtgcggt agggctacgc
     2521 ccttgggcaa tccggtactg ccactactgt tcatgatcag ggcgatggtt ttgtcccggt
     2581 cgaagctctc gggcacgaag tcgtactcgt tgaagccggg tggcaaatgg gaagtcacga
     2641 aggtgtacat gctttggaag ccctggtagt cggtcttgct atccatgatg atgatctttt
     2701 gtatgatcgg tagcttcttt tgcacgttga ggatcttttg cagccctttc ttgctcacga
     2761 atacgacggt gggctggctg atgcccatgc tgttcagcag ctcgcgctcg ttgtagatgt
     2821 cgttagctgg ggccacagcc acaccgatga acagggcacc caacacgggc atgaagaact
     2881 gcaagctatt ctcgctgcac accacgatcc gatggtttgt attcagccca tagcgcttca
     2941 tagcttctgc cagccgaacg ctcatctcga agtactcggc gtaggtaatg tccacctcga
     3001 tatgtgcgtc ggtaaaggcg atggtgccgg gcaccagggc gtagcgcttc atggctttgt
     3061 gcagctgctc gccggcggtc ccgtcttcga gtgggtagaa tggcgctggg cccttcttaa
     3121 tgtttttggc atcttccatc gaatcgatgg gatcctgcaa aaagaacaag tagcttgtat
     3181 tctatagtgt cacctaaatc aagcttgctc cgacgtcccc aggcagaatg gcggttccct
     3241 aaacgagcat tgcttatata gacctcccat taggcacgcc taccgcccat ttacgtcaat
     3301 ggaacgccca tttgcgtcat tgcccctccc cattgacgtc aatggggatg tacttggcag
     3361 ccatcgcggg ccatttaccg ccattgacgt caatgggagt actgccaatg taccctggcg
     3421 tacttccaat agtaatgtac ttgccaagtt actattaata gatattgatg tactgccaag
     3481 tgggccattt accgtcattg acgtcaatag ggggcgtgag aacggatatg aatgggcaat
     3541 gagccatccc attgacgtca atggtgggtg gtcctattga cgtcaatggg cattgagcca
     3601 ggcgggccat ttaccgtaat tgacgtcaat gggggaggcg ccatatacgt caataggacc
     3661 gcccatatga cgtcaatagg aaagaccata tatagagacc attgacgtca atgggggagt
     3721 ggctatgggc ggtattagga agccccatat atggtatatg ggaccgccca ttgggagggg
     3781 ctatctacgt caataggaaa acccatatat ggaatactat atggcatagg gccaatacat
     3841 agtattgaac ctggccaata gccatattgg catagggcca tattggatat tgcctatata
     3901 ttgatcctgg catatagcca atatggccgc cattattggc accatgccaa ttcaatatgg
     3961 cggacttggc acagggccaa gacccctcct caaacttggc accgtgccaa gtagacccct
     4021 ccccagttgg cacagtgcca ggtccgccat attgaattgg catggtgcca agtccgccat
     4081 attgaattgg ctggggtcca gatccaccat attgaattgg cacgagtcca tatacgccat
     4141 attgaattgg cacaggtcca gatccaccat attgaattgg catgagtcca tatacgccat
     4201 attgaattgg cggtcgactg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     4261 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     4321 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     4381 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     4441 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     4501 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     4561 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     4621 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     4681 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     4741 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     4801 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     4861 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     4921 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     4981 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     5041 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     5101 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     5161 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     5221 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     5281 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     5341 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     5401 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     5461 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     5521 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     5581 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     5641 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     5701 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     5761 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     5821 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     5881 ccgtagaaaa gatcaaagga tcttc
//
