LOCUS       Exported                6987 bp DNA     circular SYN 15-APR-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 6987)
  AUTHORS   ProCeD Group - Micalis
  TITLE     Direct Submission
  JOURNAL   Exported Mar 26, 2023 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6987
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     misc_feature    115..648
                     /label=amyE front
                     /note="color: #cc0000; direction: RIGHT"
     primer_bind     119..142
                     /label=AC1234
                     /note="color: black; sequence: TTGCAAAACGATTCAAAACCTCTT"
     primer_bind     638..662
                     /label=AC1304
                     /note="color: black; sequence: ATAGTACATAATGGATTTCCTTACG"
     primer_bind     731..769
                     /label=oIA001 aka AmyE up
                     /note="color: black; sequence: 
                     GCGACCGGCGCTCAGGATCgctagcgaattcgagctcgg"
     misc_feature    750..766
                     /label=ITA
                     /note="color: #ff99cc; direction: RIGHT"
     primer_bind     761..811
                     /label=oIA047
                     /note="color: black; sequence: 
                     cgagctcggtacggggatcctGCGGCCGCCATGAGTGTGATTAAACCAGAC"
     primer_bind     772..811
                     /label=oIA009 aka AmyE dronPA up
                     /note="color: black; sequence: 
                     ctcggtacccggggatcctGCGGCCGCCATGAGTGTGATTAAACCAGAC"
     CDS             791..1465
                     /codon_start=1
                     /product="monomeric photoswitchable Dronpa-Green1 
                     fluorescent protein"
                     /label=Dronpa-Green1
                     /note="color: #05fd14"
                     /translation="MSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKVK
                     EGGPLPFAYDILTTVFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMNYEDGGICNAT
                     NDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKGDVNMALSL
                     EGGGHYRCDFKTTYKAKKVVQLPDYHFVDHHIEIKSHDKDYSNVNLHEHAEAHSELPRQ
                     AK"
     primer_bind     1345..1364
                     /label=Check dronpa_N
                     /note="color: black; sequence: GCCAGACTATCACTTTGTGG"
     primer_bind     complement(1442..1488)
                     /label=oIA010 AmyE dronPA down
                     /note="color: black; sequence: 
                     gccaagcttgcatgcctgcaggtTTACTTGGCCTGCCTCGGCAGCTC"
     misc_feature    complement(1469..1491)
                     /label=ITA
                     /note="color: #ff99cc; direction: LEFT"
     primer_bind     complement(1473..1515)
                     /label=oIA002 aka AmyE down
                     /note="color: black; sequence: 
                     GATAAGCTGTCAAACATGAGAATTacagccaagcttgcatgcc"
     primer_bind     complement(1565..1585)
                     /label=AC1274
                     /note="color: black; sequence: TTAAAGGATTTGAGCGTAGCG"
     misc_feature    1804..2454
                     /label=cmR
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    2834..3853
                     /label=amyE back
                     /note="color: #cc0000; direction: RIGHT"
     primer_bind     complement(3763..3785)
                     /label=AC1235
                     /note="color: black; sequence: GCTGATTCTGACCGGGCACTTGG"
     misc_feature    complement(5917..6777)
                     /label=bla
                     /note="color: #cc0000; direction: LEFT"
ORIGIN
        1 aacaaaattc tccagtcttc acatcggttt gaaaggagga agcggaagaa tgaagtaaga
       61 gggatttttg actccgaagt aagtcttcaa aaaatcaaat aaggagtgtc aagaatgttt
      121 gcaaaacgat tcaaaacctc tttactgccg ttattcgctg gatttttatt gctgtttcat
      181 ttggttctgg caggaccggc ggctgcgagt gctgaaacgg cgaacaaatc gaatgagctt
      241 acagcaccgt cgatcaaaag cggaaccatt cttcatgcat ggaattggtc gttcaatacg
      301 ttaaaacaca atatgaagga tattcatgat gcaggatata cagccattca gacatctccg
      361 attaaccaag taaaggaagg gaatcaagga gataaaagca tgtcgaactg gtactggctg
      421 tatcagccga catcgtatca aattggcaac cgttacttag gtactgaaca agaatttaaa
      481 gaaatgtgtg cagccgctga agaatatggc ataaaggtca ttgttgacgc ggtcatcaat
      541 cataccacca gtgattatgc cgcgatttcc aatgaggtta agagtattcc aaactggaca
      601 catggaaaca cacaaattaa aaactggtct gatcgaaata gtacataatg gatttcctta
      661 cgcgaaatac gggcagacat ggcctgcccg gttattatta tttttgacac cagaccaact
      721 ggtaatggta gcgaccggcg ctcaggatcg ctagcgaatt cgagctcggt acggggatcc
      781 tgcggccgcc atgagtgtga ttaaaccaga catgaagatc aagctgcgta tggaaggcgc
      841 tgtaaatgga cacccgttcg cgattgaagg agttggcctt gggaagcctt tcgagggaaa
      901 acagagtatg gaccttaaag tcaaagaagg cggacctctg cctttcgcct atgacatctt
      961 gacaactgtg ttctgttacg gcaacagggt attcgccaaa tacccagaaa atatagtaga
     1021 ctatttcaag cagtcgtttc ctgagggcta ctcttgggaa cgaagcatga attacgaaga
     1081 cgggggcatt tgtaacgcga caaacgacat aaccctggat ggtgactgtt atatctatga
     1141 aattcgattt gatggtgtga actttcctgc caatggtcca gttatgcaga agaggactgt
     1201 gaaatgggag ccatccactg agaaattgta tgtgcgtgat ggagtgctga agggtgatgt
     1261 taacatggct ctgtcgcttg aaggaggtgg ccattaccga tgtgacttca aaactactta
     1321 taaagctaag aaggttgtcc agttgccaga ctatcacttt gtggaccacc acattgagat
     1381 taaaagccac gacaaagatt acagtaatgt taatctgcat gagcatgccg aagcgcattc
     1441 tgagctgccg aggcaggcca agtaaacctg caggcatgca agcttggctg taattctcat
     1501 gtttgacagc ttatcatcgg caatagttac ccttattatc aagataagaa agaaaaggat
     1561 ttttcgctac gctcaaatcc tttaaaaaaa cacaaaagac cacatttttt aatgtggtct
     1621 ttattcttca actaaagcac ccattagttc aacaaacgaa aattggataa agtgggatat
     1681 ttttaaaata tatatttatg ttacagtaat attgactttt aaaaaaggat tgattctaat
     1741 gaagaaagca gacaagtaag cctcctaaat tcactttaga taaaaattta ggaggcatat
     1801 caaatgaact ttaataaaat tgatttagac aattggaaga gaaaagagat atttaatcat
     1861 tatttgaacc aacaaacgac ttttagtata accacagaaa ttgatattag tgttttatac
     1921 cgaaacataa aacaagaagg atataaattt taccctgcat ttattttctt agtgacaagg
     1981 gtgataaact caaatacagc ttttagaact ggttacaata gcgacggaga gttaggttat
     2041 tgggataagt tagagccact ttatacaatt tttgatggtg tatctaaaac attctctggt
     2101 atttggactc ctgtaaagaa tgacttcaaa gagttttatg atttatacct ttctgatgta
     2161 gagaaatata atggttcggg gaaattgttt cccaaaacac ctatacctga aaatgctttt
     2221 tctctttcta ttattccatg gacttcattt actgggttta acttaaatat caataataat
     2281 agtaattacc ttctacccat tattacagca ggaaaattca ttaataaagg taattcaata
     2341 tatttaccgc tatctttaca ggtacatcat tctgtttgtg atggttatca tgcaggattg
     2401 tttatgaact ctattcagga attgtcagat aggcctaatg actggctttt ataatatgag
     2461 ataatgccga ctgtactttt tacagtcggt tttctaatgt cactaacctg ccccgttagt
     2521 tgaagaaggt ttttatatta cagctccaga tcctctacgc cggacgcatc gtggccggca
     2581 tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag
     2641 atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc
     2701 ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg
     2761 tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag
     2821 agcgtcgaca tggatgagcg atgatgatat ccgtttaggc tgggcggtga tagcttctcg
     2881 ttcaggcagt acgcctcttt tcttttccag acctgaggga ggcggaaatg gtgtgaggtt
     2941 cccggggaaa agccaaatag gcgatcgcgg gagtgcttta tttgaagatc aggctatcac
     3001 tgcggtcaat agatttcaca atgtgatggc tggacagcct gaggaactct cgaacccgaa
     3061 tggaaacaac cagatattta tgaatcagcg cggctcacat ggcgttgtgc tggcaaatgc
     3121 aggttcatcc tctgtctcta tcaatacggc aacaaaattg cctgatggca ggtatgacaa
     3181 taaagctgga gcgggttcat ttcaagtgaa cgatggtaaa ctgacaggca cgatcaatgc
     3241 caggtctgta gctgtgcttt atcctgatga tattgcaaaa gcgcctcatg ttttccttga
     3301 gaattacaaa acaggtgtaa cacattcttt caatgatcaa ctgacgatta ccttgcgtgc
     3361 agatgcgaat acaacaaaag ccgtttatca aatcaataat ggaccagacg acaggcgttt
     3421 aaggatggag atcaattcac aatcggaaaa ggagatccaa tttggcaaaa catacaccat
     3481 catgttaaaa ggaacgaaca gtgatggtgt aacgaggacc gagaaataca gttttgttaa
     3541 aagagatcca gcgtcggcca aaaccatcgg ctatcaaaat ccgaatcatt ggagccaggt
     3601 aaatgcttat atctataaac atgatgggag ccgagtaatt gaattgaccg gatcttggcc
     3661 tggaaaacca atgactaaaa atgcagacgg aatttacacg ctgacgctgc ctgcggacac
     3721 ggatacaacc aacgcaaaag tgatttttaa taatggcagc gcccaagtgc ccggtcagaa
     3781 tcagcctggc tttgattacg tgctaaatgg tttatataat gactcgggct taagcggttc
     3841 tcttccccat tgagggcaag gctagacggg acttaccgaa agaaaccatc aatgatggtt
     3901 tcttttttgt tcataaatca gacaaaactt ttctcttgca aaagtttgtg aagtgttgca
     3961 caatataaat gtgaaatact tcacaaacaa aaagacatca aagagaaaca taccctgcaa
     4021 ggatgctgat attgtctgca tttgcgccgg agcaaaccaa aaacctggtg agacacgcct
     4081 tgaattagta gaaaagaact tgaagatttt caaaggcatc gttagtgaag tcatggcgag
     4141 cggatttgac ggcattttct tagtcgcgac gcgaggctgg atggccttcc ccattatgat
     4201 tcttctcgct tccggcggca tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt
     4261 agatgacgac catcagggac agcttcaagg atcgctcgcg gctcttacca gcctaacttc
     4321 gatcactgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg
     4381 gttggcatgg attgtaggcg ccgccctata ccttgtctgc ctccccgcgt tgcgtcgcgg
     4441 tgcatggagc cgggccacct actgaagtgg atttctttaa gagctccttt aacttcctca
     4501 ccagtagttg tatcggtacc ataagtagaa gcagcaaccc aagtagcttt accagcatcc
     4561 ggttcaacca gcatagtaag aatcttactg gacatcggca gttcttcgaa cagtgcgcca
     4621 actaccagct ctttctgcag ttcattcagg gcaccggaga acctgcgtgc aatccatctt
     4681 gttcaatcat gcgaaacgat cctcatcctg tctcttgatc catggattac gcgttaaccc
     4741 gggcccgcgg atgcatatga tcagatctta aggcctaggt ctagagtctt tgttttgacg
     4801 ccattagcgt acgtaacaat cctcgttaaa ggacaaggac ctgagcggaa gtgtatcgta
     4861 cagtagacgg agtatactag tatagtctat agtccgtgga attattatat ttatctccga
     4921 cgatattctc atcagtgaaa tccagctgga gttctttagc aaattttttt attagctgaa
     4981 cttagtatta gtggccatac tcctccaatc caaagctatt tagaaagatt actatatcct
     5041 caaacaggcg gtaaccggcc tcttcatcgg gaatgcgcgc gaccttcagc atcgccggca
     5101 tgtccccctg gcggacggga agtatccagc tcgaggtcgg gccgcgttgc tggcgttttt
     5161 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     5221 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     5281 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     5341 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     5401 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     5461 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     5521 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     5581 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     5641 cggaaaaaga gttgatagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     5701 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     5761 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     5821 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     5881 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     5941 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     6001 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     6061 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     6121 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     6181 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat
     6241 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     6301 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     6361 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     6421 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     6481 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga
     6541 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     6601 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     6661 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     6721 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     6781 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     6841 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     6901 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     6961 cacgaggccc tttcgtcttc aagaatt
//
