LOCUS       Exported                8280 bp DNA     circular SYN 02-JUL-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 8280)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Mar 26, 2023 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8280
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     misc_feature    115..648
                     /label=amyE front
                     /note="color: #cc0000; direction: RIGHT"
     primer_bind     119..142
                     /label=AC1234
                     /note="color: black; sequence: TTGCAAAACGATTCAAAACCTCTT"
     primer_bind     638..662
                     /label=AC1304
                     /note="color: black; sequence: ATAGTACATAATGGATTTCCTTACG"
     primer_bind     731..769
                     /label=oIA001 aka AmyE up
                     /note="color: black; sequence: 
                     GCGACCGGCGCTCAGGATCgctagcgaattcgagctcgg"
     misc_feature    750..766
                     /label=ITA
                     /note="color: #ff99cc; direction: RIGHT"
     primer_bind     755..800
                     /label=oIA027
                     /note="color: black; sequence: 
                     cgaattcgagctcggtacggggatcGGGTTATCGAATTCCCGGTGG"
     primer_bind     761..779
                     /label=oIA047
                     /note="color: black; sequence: 
                     cgagctcggtacggggatcctGCGGCCGCCATGAGTGTGATTAAACCAGAC"
     primer_bind     1027..1055
                     /label=386-pPM10-LacI-RV
                     /note="color: black; sequence: 
                     GGATGAAAATAGAAAGAATAAAAGGAGGATGAAACCAGTAACGTTATACGATGTCG"
     primer_bind     1027..1055
                     /label=391-pAC68-LacI-RV
                     /note="color: black; sequence: 
                     AAAATGCATCTGTATTTGAAAAAGGAGGATGAAACCAGTAACGTTATACGATGTCG"
     CDS             1028..2074
                     /codon_start=1
                     /label=LacI
                     /note="color: #993366"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQL"
     primer_bind     complement(2055..2100)
                     /label=oIA058
                     /note="color: black; sequence: 
                     GGTTTAATCACACTCATGGCGGCCGCCAGCTGCATTAATGAATCGG"
     primer_bind     complement(2056..2086)
                     /label=oIA036
                     /note="color: black; sequence: 
                     CCTCCTCGCCTTTGGACATGGCGGCCGCCAGCTGCATTAATGAATCG"
     primer_bind     complement(2059..2074)
                     /label=380-mNG-5aa-LacI-FW
                     /note="color: black; sequence: 
                     TCCTCCTCTCCTTTCGAAACTCCGGAACCCTCGAGCAGCTGCATTAATGAA"
     primer_bind     2075..2104
                     /label=oIA047
                     /note="color: black; sequence: 
                     cgagctcggtacggggatcctGCGGCCGCCATGAGTGTGATTAAACCAGAC"
     primer_bind     2075..2104
                     /label=oIA009 aka AmyE dronPA up
                     /note="color: black; sequence: 
                     ctcggtacccggggatcctGCGGCCGCCATGAGTGTGATTAAACCAGAC"
     CDS             2084..2758
                     /codon_start=1
                     /product="monomeric photoswitchable Dronpa-Green1 
                     fluorescent protein"
                     /label=Dronpa-Green1
                     /note="color: #05fd14"
                     /translation="MSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKVK
                     EGGPLPFAYDILTTVFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMNYEDGGICNAT
                     NDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKGDVNMALSL
                     EGGGHYRCDFKTTYKAKKVVQLPDYHFVDHHIEIKSHDKDYSNVNLHEHAEAHSELPRQ
                     AK"
     primer_bind     complement(2185..2204)
                     /label=dronpa C
                     /note="color: black; sequence: CCATACTCTGTTTTCCCTCG"
     primer_bind     2638..2657
                     /label=Check dronpa_N
                     /note="color: black; sequence: GCCAGACTATCACTTTGTGG"
     primer_bind     complement(2735..2781)
                     /label=oIA010 AmyE dronPA down
                     /note="color: black; sequence: 
                     gccaagcttgcatgcctgcaggtTTACTTGGCCTGCCTCGGCAGCTC"
     misc_feature    complement(2762..2784)
                     /label=ITA
                     /note="color: #ff99cc; direction: LEFT"
     primer_bind     complement(2766..2808)
                     /label=oIA002 aka AmyE down
                     /note="color: black; sequence: 
                     GATAAGCTGTCAAACATGAGAATTacagccaagcttgcatgcc"
     primer_bind     complement(2858..2878)
                     /label=AC1274
                     /note="color: black; sequence: TTAAAGGATTTGAGCGTAGCG"
     misc_feature    3097..3747
                     /label=cmR
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    4127..5146
                     /label=amyE back
                     /note="color: #cc0000; direction: RIGHT"
     primer_bind     complement(5056..5078)
                     /label=AC1235
                     /note="color: black; sequence: GCTGATTCTGACCGGGCACTTGG"
     misc_feature    complement(7210..8070)
                     /label=bla
                     /note="color: #cc0000; direction: LEFT"
ORIGIN
        1 aacaaaattc tccagtcttc acatcggttt gaaaggagga agcggaagaa tgaagtaaga
       61 gggatttttg actccgaagt aagtcttcaa aaaatcaaat aaggagtgtc aagaatgttt
      121 gcaaaacgat tcaaaacctc tttactgccg ttattcgctg gatttttatt gctgtttcat
      181 ttggttctgg caggaccggc ggctgcgagt gctgaaacgg cgaacaaatc gaatgagctt
      241 acagcaccgt cgatcaaaag cggaaccatt cttcatgcat ggaattggtc gttcaatacg
      301 ttaaaacaca atatgaagga tattcatgat gcaggatata cagccattca gacatctccg
      361 attaaccaag taaaggaagg gaatcaagga gataaaagca tgtcgaactg gtactggctg
      421 tatcagccga catcgtatca aattggcaac cgttacttag gtactgaaca agaatttaaa
      481 gaaatgtgtg cagccgctga agaatatggc ataaaggtca ttgttgacgc ggtcatcaat
      541 cataccacca gtgattatgc cgcgatttcc aatgaggtta agagtattcc aaactggaca
      601 catggaaaca cacaaattaa aaactggtct gatcgaaata gtacataatg gatttcctta
      661 cgcgaaatac gggcagacat ggcctgcccg gttattatta tttttgacac cagaccaact
      721 ggtaatggta gcgaccggcg ctcaggatcg ctagcgaatt cgagctcggt acggggatcg
      781 ggttatcgaa ttcccggtgg aaacgaggtc atcatttcct tccgaaaaaa cggttgcatt
      841 taaatcttac atatgtaata ctttcaaaga ctacatttgt aagatttgat gtttgagtcg
      901 gctgaaagat cgtacgtacc aattattgtt tcgtgattgt tcaagccata acactgtagg
      961 gatagtggaa agagtgcttc atctggttac gatcaatcaa atattcaaac ggagggagac
     1021 gattttgatg aaaccagtaa cgttatacga tgtcgcagag tatgccggtg tctcttatca
     1081 gaccgtttcc cgcgtggtga accaggccag ccacgtttct gcgaaaacgc gggaaaaagt
     1141 ggaagcggcg atggcggagc tgaattacat tcccaaccgc gtggcacaac aactggcggg
     1201 caaacagtcg ttgctgattg gcgttgccac ctccagtctg gccctgcacg cgccgtcgca
     1261 aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt gccagcgtgg tggtgtcgat
     1321 ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc tcgcgcaacg
     1381 cgtcagtggg ctgatcatta actatccgct ggatgaccag gatgccattg ctgtggaagc
     1441 tgcctgcact aatgttccgg cgttatttct tgatgtctct gaccagacac ccatcaacag
     1501 tattattttc tcccatgaag acggtacgcg actgggcgtg gagcatctgg tcgcattggg
     1561 tcaccagcaa atcgcgctgt tagcgggccc attaagttct gtctcggcgc gtctgcgtct
     1621 ggctggctgg cataaatatc tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg
     1681 cgactggagt gccatgtccg gttttcaaca aaccatgcaa atgctgaatg agggcatcgt
     1741 tcccactgcg atgctggttg ccaacgatca gatggcgctg ggcgcaatgc gcgccattac
     1801 cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg ataccgaaga
     1861 cagctcatgt tatatcccgc cgttaaccac catcaaacag gattttcgcc tgctggggca
     1921 aaccagcgtg gaccgcttgc tgcaactctc tcagggccag gcggtgaagg gcaatcagct
     1981 gttgcccgtc tcactggtga aaagaaaaac caccctggcg cccaatacgc aaaccgcctc
     2041 tccccgcgcg ttggccgatt cattaatgca gctggcggcc gccatgagtg tgattaaacc
     2101 agacatgaag atcaagctgc gtatggaagg cgctgtaaat ggacacccgt tcgcgattga
     2161 aggagttggc cttgggaagc ctttcgaggg aaaacagagt atggacctta aagtcaaaga
     2221 aggcggacct ctgcctttcg cctatgacat cttgacaact gtgttctgtt acggcaacag
     2281 ggtattcgcc aaatacccag aaaatatagt agactatttc aagcagtcgt ttcctgaggg
     2341 ctactcttgg gaacgaagca tgaattacga agacgggggc atttgtaacg cgacaaacga
     2401 cataaccctg gatggtgact gttatatcta tgaaattcga tttgatggtg tgaactttcc
     2461 tgccaatggt ccagttatgc agaagaggac tgtgaaatgg gagccatcca ctgagaaatt
     2521 gtatgtgcgt gatggagtgc tgaagggtga tgttaacatg gctctgtcgc ttgaaggagg
     2581 tggccattac cgatgtgact tcaaaactac ttataaagct aagaaggttg tccagttgcc
     2641 agactatcac tttgtggacc accacattga gattaaaagc cacgacaaag attacagtaa
     2701 tgttaatctg catgagcatg ccgaagcgca ttctgagctg ccgaggcagg ccaagtaaac
     2761 ctgcaggcat gcaagcttgg ctgtaattct catgtttgac agcttatcat cggcaatagt
     2821 tacccttatt atcaagataa gaaagaaaag gatttttcgc tacgctcaaa tcctttaaaa
     2881 aaacacaaaa gaccacattt tttaatgtgg tctttattct tcaactaaag cacccattag
     2941 ttcaacaaac gaaaattgga taaagtggga tatttttaaa atatatattt atgttacagt
     3001 aatattgact tttaaaaaag gattgattct aatgaagaaa gcagacaagt aagcctccta
     3061 aattcacttt agataaaaat ttaggaggca tatcaaatga actttaataa aattgattta
     3121 gacaattgga agagaaaaga gatatttaat cattatttga accaacaaac gacttttagt
     3181 ataaccacag aaattgatat tagtgtttta taccgaaaca taaaacaaga aggatataaa
     3241 ttttaccctg catttatttt cttagtgaca agggtgataa actcaaatac agcttttaga
     3301 actggttaca atagcgacgg agagttaggt tattgggata agttagagcc actttataca
     3361 atttttgatg gtgtatctaa aacattctct ggtatttgga ctcctgtaaa gaatgacttc
     3421 aaagagtttt atgatttata cctttctgat gtagagaaat ataatggttc ggggaaattg
     3481 tttcccaaaa cacctatacc tgaaaatgct ttttctcttt ctattattcc atggacttca
     3541 tttactgggt ttaacttaaa tatcaataat aatagtaatt accttctacc cattattaca
     3601 gcaggaaaat tcattaataa aggtaattca atatatttac cgctatcttt acaggtacat
     3661 cattctgttt gtgatggtta tcatgcagga ttgtttatga actctattca ggaattgtca
     3721 gataggccta atgactggct tttataatat gagataatgc cgactgtact ttttacagtc
     3781 ggttttctaa tgtcactaac ctgccccgtt agttgaagaa ggtttttata ttacagctcc
     3841 agatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg
     3901 gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga
     3961 gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca
     4021 tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg
     4081 gctgcttcct aatgcaggag tcgcataagg gagagcgtcg acatggatga gcgatgatga
     4141 tatccgttta ggctgggcgg tgatagcttc tcgttcaggc agtacgcctc ttttcttttc
     4201 cagacctgag ggaggcggaa atggtgtgag gttcccgggg aaaagccaaa taggcgatcg
     4261 cgggagtgct ttatttgaag atcaggctat cactgcggtc aatagatttc acaatgtgat
     4321 ggctggacag cctgaggaac tctcgaaccc gaatggaaac aaccagatat ttatgaatca
     4381 gcgcggctca catggcgttg tgctggcaaa tgcaggttca tcctctgtct ctatcaatac
     4441 ggcaacaaaa ttgcctgatg gcaggtatga caataaagct ggagcgggtt catttcaagt
     4501 gaacgatggt aaactgacag gcacgatcaa tgccaggtct gtagctgtgc tttatcctga
     4561 tgatattgca aaagcgcctc atgttttcct tgagaattac aaaacaggtg taacacattc
     4621 tttcaatgat caactgacga ttaccttgcg tgcagatgcg aatacaacaa aagccgttta
     4681 tcaaatcaat aatggaccag acgacaggcg tttaaggatg gagatcaatt cacaatcgga
     4741 aaaggagatc caatttggca aaacatacac catcatgtta aaaggaacga acagtgatgg
     4801 tgtaacgagg accgagaaat acagttttgt taaaagagat ccagcgtcgg ccaaaaccat
     4861 cggctatcaa aatccgaatc attggagcca ggtaaatgct tatatctata aacatgatgg
     4921 gagccgagta attgaattga ccggatcttg gcctggaaaa ccaatgacta aaaatgcaga
     4981 cggaatttac acgctgacgc tgcctgcgga cacggataca accaacgcaa aagtgatttt
     5041 taataatggc agcgcccaag tgcccggtca gaatcagcct ggctttgatt acgtgctaaa
     5101 tggtttatat aatgactcgg gcttaagcgg ttctcttccc cattgagggc aaggctagac
     5161 gggacttacc gaaagaaacc atcaatgatg gtttcttttt tgttcataaa tcagacaaaa
     5221 cttttctctt gcaaaagttt gtgaagtgtt gcacaatata aatgtgaaat acttcacaaa
     5281 caaaaagaca tcaaagagaa acataccctg caaggatgct gatattgtct gcatttgcgc
     5341 cggagcaaac caaaaacctg gtgagacacg ccttgaatta gtagaaaaga acttgaagat
     5401 tttcaaaggc atcgttagtg aagtcatggc gagcggattt gacggcattt tcttagtcgc
     5461 gacgcgaggc tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat
     5521 gcccgcgttg caggccatgc tgtccaggca ggtagatgac gaccatcagg gacagcttca
     5581 aggatcgctc gcggctctta ccagcctaac ttcgatcact ggaccgctga tcgtcacggc
     5641 gatttatgcc gcctcggcga gcacatggaa cgggttggca tggattgtag gcgccgccct
     5701 ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca cctactgaag
     5761 tggatttctt taagagctcc tttaacttcc tcaccagtag ttgtatcggt accataagta
     5821 gaagcagcaa cccaagtagc tttaccagca tccggttcaa ccagcatagt aagaatctta
     5881 ctggacatcg gcagttcttc gaacagtgcg ccaactacca gctctttctg cagttcattc
     5941 agggcaccgg agaacctgcg tgcaatccat cttgttcaat catgcgaaac gatcctcatc
     6001 ctgtctcttg atccatggat tacgcgttaa cccgggcccg cggatgcata tgatcagatc
     6061 ttaaggccta ggtctagagt ctttgttttg acgccattag cgtacgtaac aatcctcgtt
     6121 aaaggacaag gacctgagcg gaagtgtatc gtacagtaga cggagtatac tagtatagtc
     6181 tatagtccgt ggaattatta tatttatctc cgacgatatt ctcatcagtg aaatccagct
     6241 ggagttcttt agcaaatttt tttattagct gaacttagta ttagtggcca tactcctcca
     6301 atccaaagct atttagaaag attactatat cctcaaacag gcggtaaccg gcctcttcat
     6361 cgggaatgcg cgcgaccttc agcatcgccg gcatgtcccc ctggcggacg ggaagtatcc
     6421 agctcgaggt cgggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     6481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     6541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     6601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     6661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     6721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     6781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     6841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     6901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttgata gctcttgatc
     6961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     7021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     7081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     7141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     7201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     7261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     7321 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     7381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     7441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     7501 tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat
     7561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     7621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     7681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     7741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     7801 accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt
     7861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     7921 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     7981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     8041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     8101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     8161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     8221 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaagaatt
//
