LOCUS       PC1-29-9-2_GAL4-VHL     6830 bp ds-DNA     circular     16-JUN-2022
DEFINITION  .
COMMENT     Ligation of 2 sequencesLigation of 2 sequences
FEATURES             Location/Qualifiers
     Concatenated sequence     1..4786
                               /label="221.1.1(2022reviewed) (digested by BsaI) Fragment 1 extraction (concatenated sequence 1)"
                               /ApEinfo_revcolor="#75c6a9"
                               /ApEinfo_fwdcolor="#75c6a9"
                               /note="standard_name: 221.1.1(2022reviewed) (digested by BsaI) Fragment 1 extraction (concatenated sequence 1) NCBI Feature Key: Concatenated sequence"
     misc_feature              208..305
                               /label="bla"
                               /ApEinfo_revcolor="#ffef86"
                               /ApEinfo_fwdcolor="#ffef86"
                               /note="standard_name: bla NCBI Feature Key: misc_feature"
     CDS                       306..1166
                               /label="Amp(r)"
                               /ApEinfo_revcolor="#f8d3a9"
                               /ApEinfo_fwdcolor="#f8d3a9"
                               /note="standard_name: Amp(r) NCBI Feature Key: CDS"
     rep_origin                1311..1984
                               /label="rep origin"
                               /ApEinfo_revcolor="#f58a5e"
                               /ApEinfo_fwdcolor="#f58a5e"
                               /note="NCBI Feature Key: rep_origin"
     misc_feature              2181..2424
                               /label="cHS4"
                               /ApEinfo_revcolor="#75c6a9"
                               /ApEinfo_fwdcolor="#75c6a9"
                               /note="standard_name: cHS4 NCBI Feature Key: misc_feature"
     misc_feature              2464..2707
                               /label="cHS4core"
                               /ApEinfo_revcolor="#d6b295"
                               /ApEinfo_fwdcolor="#d6b295"
                               /note="standard_name: cHS4core NCBI Feature Key: misc_feature"
     misc_feature              2954..3318
                               /label="CMVIE"
                               /ApEinfo_revcolor="#b7e6d7"
                               /ApEinfo_fwdcolor="#b7e6d7"
                               /note="standard_name: CMVIE NCBI Feature Key: misc_feature"
     misc_feature              3319..3594
                               /label="Beta Actin promoter"
                               /ApEinfo_revcolor="#f8d3a9"
                               /ApEinfo_fwdcolor="#f8d3a9"
                               /note="standard_name: Beta Actin promoter NCBI Feature Key: misc_feature"
     intron                    3691..4614
                               /label="chimeric intron"
                               /ApEinfo_revcolor="#9eafd2"
                               /ApEinfo_fwdcolor="#9eafd2"
                               /note="standard_name: chimeric intron NCBI Feature Key: intron"
     misc_feature              4727..4732
                               /label="kozark"
                               /ApEinfo_revcolor="#f58a5e"
                               /ApEinfo_fwdcolor="#f58a5e"
                               /note="standard_name: kozark NCBI Feature Key: misc_feature"
     misc_feature              4736..4756
                               /label="SV40NLS"
                               /ApEinfo_revcolor="#ff9ccd"
                               /ApEinfo_fwdcolor="#ff9ccd"
                               /note="standard_name: SV40NLS NCBI Feature Key: misc_feature"
     protein_bind              4775..4786
                               /label="gggs"
                               /ApEinfo_revcolor="#75c6a9"
                               /ApEinfo_fwdcolor="#75c6a9"
                               /note="standard_name: gggs NCBI Feature Key: protein_bind"
     ligation                  4783..4786
                               /label="Ligation"
                               /ApEinfo_revcolor="#85dae9"
                               /ApEinfo_fwdcolor="#85dae9"
                               /note="standard_name: Ligation NCBI Feature Key: ligation"
     protein_bind              4783..4786
                               /label="gggs"
                               /ApEinfo_revcolor="#84b0dc"
                               /ApEinfo_fwdcolor="#84b0dc"
                               /note="standard_name: gggs NCBI Feature Key: protein_bind"
     Editing History Insertion 4783..4786
                               /label="misc_feature"
                               /ApEinfo_revcolor="#b4abac"
                               /ApEinfo_fwdcolor="#b4abac"
                               /note="NCBI Feature Key: Editing History Insertion"
     Concatenated sequence     4783..5890
                               /label="new-GAL4-VHL (confirmed seq) extraction (concatenated sequence 2)"
                               /ApEinfo_revcolor="#f58a5e"
                               /ApEinfo_fwdcolor="#f58a5e"
                               /note="standard_name: new-GAL4-VHL (confirmed seq) extraction (concatenated sequence 2) NCBI Feature Key: Concatenated sequence"
     misc_RNA                  4787..5227
                               /label="GAL4"
                               /ApEinfo_revcolor="#b1ff67"
                               /ApEinfo_fwdcolor="#b1ff67"
                               /note="standard_name: GAL4 NCBI Feature Key: misc_RNA"
     Editing History Insertion 5212..5231
                               /label="misc_feature"
                               /ApEinfo_revcolor="#ff9ccd"
                               /ApEinfo_fwdcolor="#ff9ccd"
                               /note="NCBI Feature Key: Editing History Insertion"
     misc_RNA                  5228..5239
                               /label="linker"
                               /ApEinfo_revcolor="#c7b0e3"
                               /ApEinfo_fwdcolor="#c7b0e3"
                               /note="standard_name: linker NCBI Feature Key: misc_RNA"
     misc_RNA                  5240..5878
                               /label="VHL"
                               /ApEinfo_revcolor="#84b0dc"
                               /ApEinfo_fwdcolor="#84b0dc"
                               /note="standard_name: VHL NCBI Feature Key: misc_RNA"
     Editing History Insertion 5863..5890
                               /label="misc_feature"
                               /ApEinfo_revcolor="#c6c9d1"
                               /ApEinfo_fwdcolor="#c6c9d1"
                               /note="NCBI Feature Key: Editing History Insertion"
     Concatenated sequence     5887..6830
                               /label="221.1.1(2022reviewed) (digested by BsaI) Fragment 1 extraction (concatenated sequence 3)"
                               /ApEinfo_revcolor="#faac61"
                               /ApEinfo_fwdcolor="#faac61"
                               /note="standard_name: 221.1.1(2022reviewed) (digested by BsaI) Fragment 1 extraction (concatenated sequence 3) NCBI Feature Key: Concatenated sequence"
     ligation                  5887..5890
                               /label="Ligation"
                               /ApEinfo_revcolor="#ff9ccd"
                               /ApEinfo_fwdcolor="#ff9ccd"
                               /note="standard_name: Ligation NCBI Feature Key: ligation"
     Concatenated sequence     5891..4782
                               /label="Digestion of pA017 - Fragment 2 extraction(concatenated sequence 2)"
                               /ApEinfo_revcolor="#faac61"
                               /ApEinfo_fwdcolor="#faac61"
                               /note="standard_name: Digestion of pA017 - Fragment 2 extraction(concatenated sequence 2) NCBI Feature Key: Concatenated sequence"
     motif                     5893..5904
                               /label="GGGS linker"
                               /ApEinfo_revcolor="#faac61"
                               /ApEinfo_fwdcolor="#faac61"
                               /note="standard_name: GGGS linker NCBI Feature Key: motif"
     misc_feature              5905..5952
                               /label="NucleoplasminNLS"
                               /ApEinfo_revcolor="#d59687"
                               /ApEinfo_fwdcolor="#d59687"
                               /note="standard_name: NucleoplasminNLS NCBI Feature Key: misc_feature"
     terminator                6021..6547
                               /label="rb_glob_PA_terminator"
                               /ApEinfo_revcolor="#faac61"
                               /ApEinfo_fwdcolor="#faac61"
                               /note="standard_name: rb_glob_PA_terminator NCBI Feature Key: terminator"
ORIGIN
        1 CAGGGTAATT CGGTCAAGCC TTGCCTTGTT GTAGCTTAAA TTTTGCTCGC GCACTACTCA
       61 GCGACCTCCA ACACACAAGC AGGGAGCAGA TACTGGCTTA ACTATGCGGC ATCAGAGCAG
      121 ATTGTACTGA GAGTGCACCA TAGGGGATCG GGAGATCTCC CGATCCGTCG ACGTCAGGTG
      181 GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA
      241 ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA
      301 AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC
      361 TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG
      421 GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC
      481 GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT
      541 TATCCCGTAT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG
      601 ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG
      661 AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA
      721 CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC
      781 GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA
      841 CGATGCCTGT AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC
      901 TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC
      961 TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG
     1021 GGTCACGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA
     1081 TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG
     1141 GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA
     1201 TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC
     1261 TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA
     1321 AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA
     1381 AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC
     1441 CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTTCTTCTA GTGTAGCCGT
     1501 AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC
     1561 TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC
     1621 GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA
     1681 GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG
     1741 CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG
     1801 GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT
     1861 TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT
     1921 GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC
     1981 ACATGTTCTT TATTACCCTG TTATCCCTAA CCGGTTTACC GAGCTCTTAT TGGTTTTCAA
     2041 ACTTCATTGA CTGTGCCAAG CTTCTGACCT CTTCTCTTCC TCCCACAGGG CCTCGAGACA
     2101 ATTGCATCGA TGGTACCAGT GAAAGTCTGC AATGAATTCG AGTTGGCGCG CCTGTCATTC
     2161 TAAATCTCTC TTTCAGCCTA AAGCTTTTTC CCCGTATCCC CCCAGGTGTC TGCAGGCTCA
     2221 AAGAGCAGCG AGAAGCGTTC AGAGGAAAGC GATCCCGTGC CACCTTCCCC GTGCCCGGGC
     2281 TGTCCCCGCA CGCTGCCGGC TCGGGGATGC GGGGGGAGCG CCGGACCGGA GCGGAGCCCC
     2341 GGGCGGCTCG CTGCTGCCCC CTAGCGGGGG AGGGACGTAA TTACATCCCT GGGGGCTTTG
     2401 GGAGGGGGCT GTCCCCGTGA GCTCCCAGGC GCGCCTGTCA TTCTAAATCT CTCTTTCAGC
     2461 CTAAAGCTTT TTCCCCGTAT CCCCCCAGGT GTCTGCAGGC TCAAAGAGCA GCGAGAAGCG
     2521 TTCAGAGGAA AGCGATCCCG TGCCACCTTC CCCGTGCCCG GGCTGTCCCC GCACGCTGCC
     2581 GGCTCGGGGA TGCGGGGGGA GCGCCGGACC GGAGCGGAGC CCCGGGCGGC TCGCTGCTGC
     2641 CCCCTAGCGG GGGAGGGACG TAATTACATC CCTGGGGGCT TTGGGGGGGG GCTGTCCCCG
     2701 TGAGCTCCCA GGCGCGCCAA CTCGGTACCG TATCGATGTC GACGTTAACG CTAGTGATAT
     2761 CAACTTTGTA TAGAAAAGTT GGCTCCGAAT TTCTCGAGTT AATTAAATGT GCTGCAAGGC
     2821 GATTAAGTTG GGTAACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTG
     2881 AATTGTAATA CGACTCACTA TAGGGCGAAT TGGAGCTAGG CCTACGTAGC GCGCGAGCTC
     2941 CACCGCGGTG GACTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT
     3001 ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA CCGCCCAACG
     3061 ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT AGTAACGCCA ATAGGGACTT
     3121 TCCATTGACG TCAATGGGTG GAGTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG
     3181 TGTATCATAT GCCAAGTACG CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC
     3241 ATTATGCCCA GTACATGACC TTATGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG
     3301 TCATCGCTAT TACCATGGTC GAGGTGAGCC CCACGTTCTG CTTCACTCTC CCCATCTCCC
     3361 CCCCCTCCCC ACCCCCAATT TTGTATTTAT TTATTTTTTA ATTATTTTGT GCAGCGATGG
     3421 GGGCGGGGGG GGGGGGGGCG CGCGCCAGGC GGGGCGGGGC GGGGCGAGGG GCGGGGCGGG
     3481 GCGAGGCGGA GAGGTGCGGC GGCAGCCAAT CAGAGCGGCG CGCTCCGAAA GTTTCCTTTT
     3541 ATGGCGAGGC GGCGGCGGCG GCGGCCCTAT AAAAAGCGAA GCGCGCGGCG GGCGGGAGTC
     3601 GAGTCGCTGC GTTGCCTTCG CCCCGTGCCC CGCTCCGCCG CCGCCTCGCG CCGCCCGCCC
     3661 CGGCTCTGAC TGACCGCGTT ACTCCCACAG GTGAGCGGGC GGGACGGCCC TTCTCCTCCG
     3721 GGCTGTAATT AGCGCTTGGT TTAATGACGG CTTGTTTCTT TTCTGTGGCT GCGTGAAAGC
     3781 CTTGAGGGGC TCCGGGAGGG CCCTTTGTGC GGGGGGAGCG GCTCGGGGGG TGCGTGCGTG
     3841 TGTGTGTGCG TGGGGAGCGC CGCGTGCGGC TCCGCGCTGC CCGGCGGCTG TGAGCGCTGC
     3901 GGGCGCGGCG CGGGGCTTTG TGCGCTCCGC AGTGTGCGCG AGGGGAGCGC GGCCGGGGGC
     3961 GGTGCCCCGC GGTGCGGGGG GGGCTGCGAG GGGAACAAAG GCTGCGGGCG GGGTGTGTGC
     4021 GTGGGGGGGT GAGCAGGGGG TGTGGGCGCG TCGGTCGGGC TGCAACCCCC CCTGCACCCC
     4081 CCTCCCCGAG TTGCTGAGCA CGGCCCGGCT TCGGGTGCGG GGCTCCGTAC GGGGCGTGGC
     4141 GCGGGGCTCG CCGTGCCGGG CGGGGGGTGG CGGCAGGTGG GGGTGCCGGG CGGGGCGGGG
     4201 CCGCCTCGGG CCGGGGAGGG CTCGGGGGAG GGGCGCGGCG GCCCCCGGAG CGCCGGCGGC
     4261 TGTCGAGGCG CGGCGAGCCG CAGCCATTGC CTTTTATGGT AATCGTGCGA GAGGGCGCAG
     4321 GGACTTCCTT TGTCCCAAAT CTGTGCGGAG CCGAAATCTG GGAGGCGCCG CCGCACCCCC
     4381 TCTAGCGGGC GCGGGGCGAA GCGGTGCGGC GCCGGCAGGA AGGAAATGGG CGGGGAGGGC
     4441 CTTCGTGCGT CGCCGCGCCG CCGTCCCCTT CTCCCTCTCC AGCCTCGGGG CTGTCCGCGG
     4501 GGGGACGGCT GCCTTCGGGG GGGACGGGGC AGGGCGGGGT TCGGCTTCTG GCGTGTGACC
     4561 GGCGGCTCTA GAGCCTCTGC TAACCATGTT CATGCCTTCT TCTTTTTCCT ACAGCTCCTG
     4621 GGCAACGTGC TGGTTATTGT GCTGTCTCAT CATTTTGGCA AAGAATTCGA CCCAAGTTTG
     4681 TACAAAAAAG CAGGCTGATT ACCGGAGAAT TGGCGGCCGC ACCGGTGCCA CCATGCCAAA
     4741 GAAGAAGCGG AAGGTCGGTG GCGGCTCACC CGGGGGTGGC GGCTCAATGA AGCTACTGTC
     4801 TTCTATCGAA CAAGCATGCG ATATTTGCCG ACTTAAAAAG CTCAAGTGCT CCAAAGAAAA
     4861 ACCGAAGTGC GCCAAGTGTC TGAAGAACAA CTGGGAGTGT CGCTACTCTC CCAAAACCAA
     4921 AAGGTCGCCG CTGACTAGGG CACATCTGAC AGAAGTGGAA TCAAGGCTAG AAAGACTGGA
     4981 ACAGCTATTT CTACTGATTT TTCCTCGAGA AGACCTTGAC ATGATTTTGA AAATGGATTC
     5041 TTTACAGGAT ATAAAAGCAT TGTTAACAGG ATTATTTGTA CAAGATAATG TGAATAAAGA
     5101 TGCCGTCACA GATAGATTGG CTTCAGTGGA GACTGATATG CCTCTAACAT TGAGACAGCA
     5161 TAGAATAAGT GCGACATCAT CATCGGAAGA GAGTAGTAAC AAAGGTCAAA GACAGTTGAC
     5221 TGTATCGGGT GGCGGCTCAA TGCCCCGGAG GGCGGAGAAC TGGGACGAGG CCGAGGTAGG
     5281 CGCGGAGGAG GCAGGCGTCG AAGAGTACGG CCCTGAAGAA GACGGCGGGG AGGAGTCGGG
     5341 CGCCGAGGAG TCCGGCCCGG AAGAGTCCGG CCCGGAGGAA CTGGGCGCCG AGGAGGAGAT
     5401 GGAGGCCGGG CGGCCGCGGC CCGTGCTGCG CTCGGTGAAC TCGCGCGAGC CCTCCCAGGT
     5461 CATCTTCTGC AATCGCAGTC CGCGCGTCGT GCTGCCCGTA TGGCTCAACT TCGACGGCGA
     5521 GCCGCAGCCC TACCCAACGC TGCCGCCTGG CACGGGCCGC CGCATCCACA GCTACCGAGG
     5581 TCACCTTTGG CTCTTCAGAG ATGCAGGGAC ACACGATGGG CTTCTGGTTA ACCAAACTGA
     5641 ATTATTTGTG CCATCTCTCA ATGTTGACGG ACAGCCTATT TTTGCCAATA TCACACTGCC
     5701 AGTGTATACT CTGAAAGAGC GATGCCTCCA GGTTGTCCGG AGCCTAGTCA AGCCTGAGAA
     5761 TTACAGGAGA CTGGACATCG TCAGGTCGCT CTACGAAGAT CTGGAAGACC ACCCAAATGT
     5821 GCAGAAAGAC CTGGAGCGGC TGACACAGGA GCGCATTGCA CATCAACGGA TGGGAGATTG
     5881 ATTGCCACTA GTGGTGGCGG CTCAAAGCGT CCTGCTGCTA CTAAGAAAGC TGGTCAAGCT
     5941 AAGAAAAAGA AATCTAGATA GTGACTCGAG ACTAGATAAC TGATCTACCC AGCTTTCTTG
     6001 TACAAAGTGG TACGCGTGAA TTCACTCCTC AGGTGCAGGC TGCCTATCAG AAGGTGGTGG
     6061 CTGGTGTGGC CAATGCCCTG GCTCACAAAT ACCACTGAGA TCTTTTTCCC TCTGCCAAAA
     6121 ATTATGGGGA CATCATGAAG CCCCTTGAGC ATCTGACTTC TGGCTAATAA AGGAAATTTA
     6181 TTTTCATTGC AATAGTGTGT TGGAATTTTT TGTGTCTCTC ACTCGGAAGG ACATATGGGA
     6241 GGGCAAATCA TTTAAAACAT CAGAATGAGT ATTTGGTTTA GAGTTTGGCA ACATATGCCC
     6301 ATATGCTGGC TGCCATGAAC AAAGGTTGGC TATAAAGAGG TCATCAGTAT ATGAAACAGC
     6361 CCCCTGCTGT CCATTCCTTA TTCCATAGAA AAGCCTTGAC TTGAGGTTAG ATTTTTTTTA
     6421 TATTTTGTTT TGTGTTATTT TTTTCTTTAA CATCCCTAAA ATTTTCCTTA CATGTTTTAC
     6481 TAGCCAGATT TTTCCTCCTC TCCTGACTAC TCCCAGTCAT AGCTGTCCCT CTTCTCTTAT
     6541 GGAGATCCCT CGACCTGCAG CCCAAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG
     6601 TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA
     6661 GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT
     6721 TTCCAGTCGG GAAACCTGTC GTGCCAGCGG ATCGACAGTA CTAAGCTTGG TGCGTTTTTA
     6781 TGCTTGTAGT ATTGTATAAT GTTTTTAAGA TCCTTAATTA ATAGGGATAA
//