LOCUS       Exported                6423 bp ds-DNA     circular SYN 16-FEB-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6423)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 4, 2023 from SnapGene 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6423
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      2..88
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
                     /note="color: #ffffff"
     primer_bind     22..41
                     /label=A423 rrnb T1 ter -f
                     /note="color: black; sequence: cgttcaccgacaaacaacag; added:
                     2022-07-25"
     primer_bind     128..147
                     /label=C133 pME 5' con -f
                     /note="color: black; sequence: ggatgttttcccagtcacga; added:
                     2022-07-25"
     primer_bind     137..159
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
                     /note="color: black; sequence: CCCAGTCACGACGTTGTAAAACG"
     primer_bind     complement(148..168)
                     /label=A1173 2a-CreER-vector-r
                     /note="color: black; sequence: actggccgtcgttttacaacg; 
                     added: 2023-02-16"
     primer_bind     151..168
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
                     /note="color: black; sequence: TGTAAAACGACGGCCAGT"
     primer_bind     152..168
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     183..217
                     /label=P2A-BsaI-AGTC-ORF-3-f
                     /note="color: black; sequence: 
                     atcggtctctAGTCccgctacaaacttctctctgctg; added: 2022-08-02"
     primer_bind     183..217
                     /label=P2A-BsaI-AGTC-ORF-2-f
                     /note="color: black; sequence: 
                     atcggtctctAGTCcgctacaaacttctctctgctg; added: 2022-08-02"
     primer_bind     183..217
                     /label=P2A-BsaI-AGTC-ORF-1-f
                     /note="color: black; sequence: 
                     atcggtctctAGTCgctacaaacttctctctgctg; added: 2022-08-02"
     primer_bind     complement(183..194)
                     /label=BsaI-CreER-vec-r
                     /note="color: black; sequence: 
                     gtacggtcagtaaattggacatggtggcGACTagagaccg; added: 
                     2022-08-09"
     CDS             197..253
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /note="color: #cc99b2
                     Cleavage site after base 251"
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(231..270)
                     /label=A1174 2a-CreER-2a-r
                     /note="color: black; sequence: 
                     acggtcagtaaattggaaggtccagggttctcctccacgt; added: 
                     2023-02-16"
     primer_bind     234..276
                     /label=A1172 2a-CreER-vector-f
                     /note="color: black; sequence: 
                     tggaggagaaccctggaccttccaatttactgaccgtacacca; added: 
                     2023-02-16"
     CDS             254..1279
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /note="color: #993366"
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLL
                     SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
                     GD"
     primer_bind     254..276
                     /label=Cre KpnI -f
                     /note="color: black; sequence: 
                     ccggtaccatgtccaatttactgaccgtacacca"
     primer_bind     254..276
                     /label=CreER-BsaI-ins-f
                     /note="color: black; sequence: 
                     cggtctctAGTCgccaccatgtccaatttactgaccgtacacca; added: 
                     2022-08-09"
     misc_feature    809..828
                     /label=Cre scr -f
                     /note="color: #a6acb3"
     primer_bind     complement(908..917)
                     /label=A228 EGFP-N
                     /note="color: black; sequence: cgtcgccgtccagctcgaccag; 
                     added: 2022-02-04"
     misc_feature    complement(988..1008)
                     /label=Cre scr -r
                     /note="color: #a6acb3; direction: LEFT"
     primer_bind     complement(988..1008)
                     /label=C38 Cre Scr -r
                     /note="color: black; sequence: gtggcagatggcgcggcaaca"
     primer_bind     complement(1260..1279)
                     /label=Cre-stop-r
                     /note="color: black; sequence: ctaatcgccatcttccagcaggc"
     CDS             1292..2227
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /note="color: #993366"
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     primer_bind     complement(1590..1625)
                     /label=A1051ERT2 BsaI mut in fusion -r
                     /note="color: black; sequence: 
                     gccagacgagGccaatcatcaggatctctagccagg; added: 2022-08-09"
     primer_bind     1606..1635
                     /label=A1050 ERT2 BsaI mut in fusion -f
                     /note="color: black; sequence: 
                     gatgattggCctcgtctggcgctccatgga; added: 2022-08-09"
     primer_bind     2213..2258
                     /label=BsaI-CreER-vec-f
                     /note="color: black; sequence: 
                     ggtttccctgccacagcttaatctagaactatagtgagtcgtatta; added: 
                     2022-08-09"
     primer_bind     complement(2213..2258)
                     /label=CreER-BsaI-ins-r
                     /note="color: black; sequence: 
                     taatacgactcactatagttctagattaagctgtggcagggaaacc; added: 
                     2022-08-09"
     primer_bind     complement(2213..2233)
                     /label=ERT2 SacI -r
                     /note="color: black; sequence: 
                     cgagagctcttaagctgtggcagggaaacc"
     promoter        complement(2240..2258)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     polyA_signal    2263..2397
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     2407..2459
                     /label=FRT EcoRI-f
                     /note="color: black; sequence: 
                     gaggaattcgaagttcctattctctagaaagtataggaacttctaagatacattgat; 
                     added: 2022-08-09"
     primer_bind     2409..2430
                     /label=C154 pKHR -f NheI
                     /note="color: black; sequence: 
                     gccgctagcttcgaagttcctattctctaga"
     primer_bind     2412..2461
                     /label=C252 FRT-a-cry ClaI -f
                     /note="color: black; sequence: 
                     cgatatcgatgaagttcctattctctagaaagtataggaacttctaagatacattgatg
                     a"
     protein_bind    2412..2445
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
                     /note="color: #31849b; direction: RIGHT"
     polyA_signal    2446..2567
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(2611..2630)
                     /label=SV40-pA-BsaI-CTCA-r
                     /note="color: black; sequence: 
                     gatggtctctTGAGgaattaaaaaacctcccacacct; added: 2022-08-02"
     CDS             complement(2690..3400)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /note="color: #ff0000"
                     /translation="MVSKGEDDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFARDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGHHSTGGMDELYK"
     primer_bind     2691..2710
                     /label=C17 mCherry stop XhoI -r
                     /note="color: black; sequence: 
                     cctcgagctacttgtacagctcgtccat; added: 2022-07-27"
     primer_bind     2691..2709
                     /label=C16 gfp stop XhoI -r
                     /note="color: black; sequence: cctcgagctacttgtacagctcgtcca;
                     added: 2022-07-27"
     primer_bind     complement(2770..2793)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
                     /note="color: black; sequence: AGCTGGACATCACCTCCCACAACG"
     primer_bind     complement(2968..2987)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
                     /note="color: black; sequence: CCCCGTAATGCAGAAGAAGA"
     primer_bind     3237..3255
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
                     /note="color: black; sequence: TTGGTCACCTTCAGCTTGG"
     primer_bind     3336..3355
                     /label=C200 mCherry con -r
                     /note="color: black; sequence: tgcaccttgaagcgcatgaa"
     primer_bind     complement(3381..3407)
                     /label=A836 mCherry-kozak-ATG KpnI -f
                     /note="color: black; sequence: 
                     ccggtaccgccaccatggtgagcaagggcgagga; added: 2022-07-27"
     primer_bind     complement(3381..3401)
                     /label=C8 GFP SacI -f
                     /note="color: black; sequence: cgagctcATGgtgagcaagggcgagga;
                     added: 2022-07-27"
     regulatory      3397..3406
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
     misc_feature    complement(3413..4041)
                     /label=a-cry-promoter
                     /note="color: #a6acb3; direction: LEFT"
     primer_bind     3508..3533
                     /label=C85 a-cry EcoRI -r
                     /note="color: black; sequence: 
                     cccgaattcacagtacaaagatccccaaatgatgt"
     primer_bind     complement(4026..4080)
                     /label=FRT EcoRI -r
                     /note="color: black; sequence: 
                     gaggaattcgaagttcctatactttctagagaataggaacttcaaaattgtgaaatgca
                     ; added: 2022-08-09"
     primer_bind     complement(4026..4076)
                     /label=C253 FRT-a-cry BamHI -r
                     /note="color: black; sequence: 
                     ggcggatccgaagttcctatactttctagagaataggaacttcaaaattgtgaaatgca
                     "
     protein_bind    4042..4075
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
                     /note="color: #31849b; direction: RIGHT"
     primer_bind     4042..4060
                     /label=C154 pKHR -f NheI
                     /note="color: black; sequence: 
                     gccgctagcttcgaagttcctattctctaga"
     primer_bind     complement(4111..4144)
                     /label=SV40-pA-BsaI-CTCA-r
                     /note="color: black; sequence: 
                     gatggtctctTGAGgaattaaaaaacctcccacacct; added: 2022-08-02"
     CDS             4504..5295
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /note="color: #ccffcc"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5388..5976
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     primer_bind     5877..5896
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
                     /note="color: black; sequence: GGGAAACGCCTGGTATCTTT"
     primer_bind     6130..6147
                     /label=A757 L4440
                     /note="L4440 vector, forward primer"
                     /note="color: black; sequence: AGCGAGTCAGTGAGCGAG"
     terminator      6306..6333
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
                     /note="color: #ffffff"
ORIGIN
        1 gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt
       61 cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct cgcgttaacg
      121 ctagcatgga tgttttccca gtcacgacgt tgtaaaacga cggccagtct taagctcggg
      181 cccggtctct agtcccgcta caaacttctc tctgctgaag caggctggag acgtggagga
      241 gaaccctgga ccttccaatt tactgaccgt acaccaaaat ttgcctgcat taccggtcga
      301 tgcaacgagt gatgaggttc gcaagaacct gatggacatg ttcagggatc gccaggcgtt
      361 ttctgagcat acctggaaaa tgcttctgtc cgtttgccgg tcgtgggcgg catggtgcaa
      421 gttgaataac cggaaatggt ttcccgcaga acctgaagat gttcgcgatt atcttctata
      481 tcttcaggcg cgcggtctgg cagtaaaaac tatccagcaa catttgggcc agctaaacat
      541 gcttcatcgt cggtccgggc tgccacgacc aagtgacagc aatgctgttt cactggttat
      601 gcggcggatc cgaaaagaaa acgttgatgc cggtgaacgt gcaaaacagg ctctagcgtt
      661 cgaacgcact gatttcgacc aggttcgttc actcatggaa aatagcgatc gctgccagga
      721 tatacgtaat ctggcatttc tggggattgc ttataacacc ctgttacgta tagccgaaat
      781 tgccaggatc agggttaaag atatctcacg tactgacggt gggagaatgt taatccatat
      841 tggcagaacg aaaacgctgg ttagcaccgc aggtgtagag aaggcactta gcctgggggt
      901 aactaaactg gtcgagcgat ggatttccgt ctctggtgta gctgatgatc cgaataacta
      961 cctgttttgc cgggtcagaa aaaatggtgt tgccgcgcca tctgccacca gccagctatc
     1021 aactcgcgcc ctggaaggga tttttgaagc aactcatcga ttgatttacg gcgctaagga
     1081 tgactctggt cagagatacc tggcctggtc tggacacagt gcccgtgtcg gagccgcgcg
     1141 agatatggcc cgcgctggag tttcaatacc ggagatcatg caagctggtg gctggaccaa
     1201 tgtaaatatt gtcatgaact atatccgtaa cctggatagt gaaacagggg caatggtgcg
     1261 cctgctggaa gatggcgatc tcgagccatc tgctggagac atgagagctg ccaacctttg
     1321 gccaagcccg ctcatgatca aacgctctaa gaagaacagc ctggccttgt ccctgacggc
     1381 cgaccagatg gtcagtgcct tgttggatgc tgagcccccc atactctatt ccgagtatga
     1441 tcctaccaga cccttcagtg aagcttcgat gatgggctta ctgaccaacc tggcagacag
     1501 ggagctggtt cacatgatca actgggcgaa gagggtgcca ggctttgtgg atttgaccct
     1561 ccatgatcag gtccaccttc tagaatgtgc ctggctagag atcctgatga ttggcctcgt
     1621 ctggcgctcc atggagcacc cagtgaagct actgtttgct cctaacttgc tcttggacag
     1681 gaaccaggga aaatgtgtag agggcatggt ggagatcttc gacatgctgc tggctacatc
     1741 atctcggttc cgcatgatga atctgcaggg agaggagttt gtgtgcctca aatctattat
     1801 tttgcttaat tctggagtgt acacatttct gtccagcacc ctgaagtctc tggaagagaa
     1861 ggaccatatc caccgagtcc tggacaagat cacagacact ttgatccacc tgatggccaa
     1921 ggcaggcctg accctgcagc agcagcacca gcggctggcc cagctcctcc tcatcctctc
     1981 ccacatcagg cacatgagta acaaaggcat ggagcatctg tacagcatga agtgcaagaa
     2041 cgtggtgccc ctctatgacc tgctgctgga ggcggcggac gcccaccgcc tacatgcgcc
     2101 cactagccgt ggaggggcat ccgtggagga gacggaccaa agccacttgg ccactgcggg
     2161 ctctacttca tcgcattcct tgcaaaagta ttacatcacg ggggaggcag agggtttccc
     2221 tgccacagct taatctagaa ctatagtgag tcgtattacg tagatccaga catgataaga
     2281 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     2341 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     2401 aacaacaatt cgaagttcct attctctaga aagtatagga acttctaaga tacattgatg
     2461 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     2521 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     2581 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa
     2641 acctctacaa atgtggtatg gctgattatg atctagagtc gcggccgctt tacttgtaca
     2701 gctcgtccat gccgccggtg gagtggtggc cctcggcgcg ctcgtactgt tccacgatgg
     2761 tgtagtcctc gttgtgggag gtgatgtcca gcttgatgtt gacgttgtag gcgccgggca
     2821 gctgcacggg cttcttggcc ttgtaggtgg tcttgacctc ggcgtcgtag tggccgccgt
     2881 ccttcagctt cagcctctgc ttgatctcgc ccttcagggc gccgtcctcg gggtacatcc
     2941 gctcggagga ggcctcccag cccatggtct tcttctgcat tacggggccg tcggagggga
     3001 agttggtgcc gcgcagcttc accttgtaga tgaactcgcc gtcctgcagg gaggagtcct
     3061 gggtcacggt caccacgccg ccgtcctcga agttcatcac gcgctcccac ttgaagccct
     3121 cggggaagga cagcttcaag tagtcgggga tgtcggcggg gtgcttcacg taggccttgg
     3181 agccgtacat gaactgaggg gacaggatgt cccgggcgaa gggcaggggg ccgcccttgg
     3241 tcaccttcag cttggcggtc tgggtgccct cgtaggggcg gccctcgccc tcgccctcga
     3301 tctcgaactc gtggccgttc acggagccct ccatgtgcac cttgaagcgc atgaactcct
     3361 tgatgatggc catgttgtca tcctcgccct tgctcaccat ggtggcgacc ggtaatgtca
     3421 gacctggtaa ctccttactg taacttattg cacctgatga acaaaagatg tgaactgaac
     3481 gcaagtagga ggctaataga ggctagtaca gtacaaagat ccccaaatga tgtgtgccgg
     3541 ccttagtgga ggcagacccc tggttatata cctgtgctgc agaaagaggg gcttacaaat
     3601 ggatctctct ggaatggcat tatctcatgg tgggaacagt gttgtcagca gaatcagcgg
     3661 ggtggactct gacctgagaa tgacagtcta gcactgaatg gctcagacaa tactcttgtg
     3721 gccaactatc accggaaaat aagtgcctgc caaaacaaac cagaccagat gaagctacat
     3781 ggctgtagaa cgtcagtatt taatgtgttg tggccacttg gttcaccaga tttaagagct
     3841 aattttttta aatatcacag ataatcaatc gctttttttt ttcttgactg aaaagcttag
     3901 actggaaaat tctttgaaaa agtttgcact acagaaaata aatacataac atcctgcact
     3961 gaatgcacac tattaataga aaagagagca ttattgtagg aaatgtattg tatatgtaca
     4021 ttctgtgcat ttcacaattt tgaagttcct attctctaga aagtatagga acttcgaatt
     4081 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa ttcctcaaga
     4141 gacctctaga ccagccagga cagaaatgcc tcgacttcgc tgctacccaa ggttgccggg
     4201 tgacgcacac cgtggaaacg gatgaaggca cgaacccagt ggacataagc ctgttcggtt
     4261 cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa
     4321 cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg
     4381 gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg
     4441 atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac
     4501 attatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc
     4561 atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat
     4621 ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat
     4681 gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag
     4741 agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg
     4801 cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca
     4861 ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga
     4921 gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa
     4981 caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg
     5041 gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc
     5101 ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag
     5161 tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg
     5221 gcctcgcgcg cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag
     5281 gtagtcggca aataaccctc gagccaccca tgaccaaaat cccttaacgt gagttacgcg
     5341 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     5401 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     5461 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     5521 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     5581 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     5641 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     5701 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     5761 agatacctac agcgtgagca ttgagaaagc gccacgcttc ccgaagggag aaaggcggac
     5821 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     5881 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     5941 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     6001 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     6061 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     6121 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     6181 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     6241 gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa
     6301 acgcaaaaag gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg
     6361 gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg
     6421 gcg
//
