LOCUS       Exported                7237 bp DNA     circular SYN 28-MAR-2023
DEFINITION  .
ACCESSION   <unknown
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7237)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 28, 2023 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7237
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(171..1031)
                     /codon_start=1
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #ccffcc"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        1032..1136
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff"
     misc_feature    1195..1247
                     /label=bom
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=37.5886524822695"
                     /note="/other=misc feature"
                     /note="color: #a6acb3; direction: RIGHT"
     enhancer        1402..1781
                     /label=CMV enhancer
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=enhancer"
                     /note="color: #a6acb3; direction: RIGHT"
     promoter        1782..1985
                     /label=CMV promoter
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: RIGHT"
     LTR             1981..2014
                     /label=5' LTR (truncated)
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=97.059"
                     /note="/match_length=18.88888888888889"
                     /note="/other=LTR"
                     /note="color: #ffe4c4"
     promoter        2030..2048
                     /label=T7 promoter
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: RIGHT"
     protein_bind    2078..2102
                     /label=attB1
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein bind"
                     /note="color: #31849b; direction: RIGHT"
     CDS             2129..3832
                     /codon_start=1
                     /label=CES2C-delta C
                     /note="color: #ff0000"
                     /translation="MTRNQLHNWLNAGFFGLLLLLIHVQGDYKDDDDKGGGQDSPEANP
                     IRNTHTGQIQGSLIHVKDTKAGVHTFLGIPFAKPPVGPLRFAPPEAPEPWSGVRDGTAH
                     PAMCLQNLDMLNEAGLPDMKMMLSSFPMSEDCLYLNIYTPAHAHEGSNLPVMVWIHGGA
                     LVIGMASMFDGSLLTVNEDLVVVTIQYRLGVLGFFSTGDQHARGNWGYLDQAAALRWVQ
                     QNIAHFGGNPDRVTIFGESAGGTSVSSHVVSPMSQGLFHGAIMESGVALLPDLISETSE
                     MVSTTVAKLSGCEAMDSQALVRCLRGKSEAEILAINKVFKMIPAVVDGEFFPRHPKELL
                     ASEDFHPVPSIIGVNNDEFGWSIPVVMGSAQMIKGITRENLQAVLKDTAVQMMLPPECS
                     DLLMEEYMGDTEDAQTLQIQFTEMMGDFMFVIPALQVAHFQRSHAPVYFYEFQHPPSYF
                     KDVRPPHVKADHADEIPFVFASFFWGMKLDFTEEEELLSRRMMKYWANFARHGNPNSEG
                     LPYWPVMDHDEQYLQLDIQPAVGRALKAGRLQFWTKTLPQKIQELKASQDK"
     misc_feature    2129..2206
                     /label=Signal peptide
                     /note="color: #00ffff"
     misc_feature    2207..2239
                     /label=FLAG-linker
                     /note="color: #85dae9"
     misc_feature    3620..3625
                     /label=RR cleavage site
                     /note="color: #00ffff"
     primer_bind     complement(3802..3832)
                     /label=Primer 1a
                     /note="color: black; sequence: 
                     aaactaCTTGTCCTGAGAAGCCTTTAGCTCCTGGATC; added: 2021-08-24"
     primer_bind     complement(3805..3832)
                     /label=Primer 1
                     /note="color: black; sequence: 
                     aaactacttgtcctgagaacccttgagctcctgg; added: 2021-08-24"
     protein_bind    3852..3876
                     /label=attB2
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein bind"
                     /note="color: #31849b"
     CDS             3902..3943
                     /codon_start=1
                     /label=V5 tag
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="GKPIPNPLLGLDST"
     CDS             3952..3970
                     /codon_start=1
                     /label=8xHis
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=79.16666666666666"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="SSSPSP"
     polyA_signal    3999..4223
                     /label=bGH poly(A) signal
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA signal"
                     /note="color: #a6acb3; direction: RIGHT"
     promoter        4227..4248
                     /label=SV40 promoter
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=6.197183098591549"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      4269..4697
                     /direction=RIGHT
                     /label=f1 ori
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
                     /note="color: #ffff00"
     promoter        4711..5040
                     /label=SV40 promoter
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             5107..5901
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    6077..6207
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=98.496"
                     /note="/match_length=97.08029197080292"
                     /note="/other=polyA signal"
                     /note="color: #a6acb3"
     protein_bind    6269..6291
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=95.83333333333334"
                     /note="/other=protein bind"
                     /note="color: #31849b; direction: RIGHT"
     promoter        6295..6325
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=96.875"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff"
     protein_bind    6340..6361
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein bind"
                     /note="color: #31849b; direction: RIGHT"
     CDS             complement(6374..6467)
                     /codon_start=1
                     /label=lacI
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.679593721144968"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="SAQYANRLSPRVGRFINAAGTTGFPTGKRAV"
     rep_origin      6649..7237
                     /label=ori
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
                     /note="color: #ffff00"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt
     1201 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
     1261 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
     1321 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
     1381 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
     1441 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
     1501 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
     1561 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
     1621 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
     1681 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
     1741 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
     1801 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
     1861 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
     1921 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
     1981 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
     2041 actataggga gacccaagct ggctagttaa gctatcaaca agtttgtaca aaaaagcagg
     2101 ctccgcggcc gcccccttca ccgccaccat gacacggaac caactacata actggctgaa
     2161 tgctgggttc ttcgggctgc tgcttcttct tatccatgtg cagggtgact acaaggatga
     2221 cgacgataag gggggcggtc aggactcacc agaggccaac cccatcagaa acacacatac
     2281 aggacagatc caaggcagcc ttatccacgt gaaggacact aaagctggtg tccacacctt
     2341 cctgggaatc ccctttgcca aacctcctgt aggaccactg cgatttgcac ctcctgaggc
     2401 ccctgaacca tggagtggtg tgagagatgg aactgcacat ccggccatgt gtctgcaaaa
     2461 tcttgacatg ctgaatgaag caggcctgcc agatatgaaa atgatgctgt cttccttccc
     2521 tatgtctgag gactgcctgt atctcaacat ctacacccca gcccatgccc atgagggctc
     2581 taacctgcct gtgatggtgt ggatccatgg aggtgcactg gttataggaa tggcttccat
     2641 gtttgatgga tctctattga cagtcaatga ggacttggtg gttgttacta tccagtatcg
     2701 tctgggtgtc ctgggctttt tcagcactgg agaccagcat gccagaggca actggggata
     2761 cctggaccaa gcagctgccc tacgctgggt ccagcagaac atcgctcact ttggaggcaa
     2821 ccctgaccgg gtcactattt ttggcgagtc tgcaggtggc acaagtgtgt cttcacatgt
     2881 tgtgtccccc atgtcccaag ggctcttcca tggtgccatc atggagagtg gggtggccct
     2941 gcttcctgac cttatctctg aaacctctga gatggtttcc actacggtgg ccaagctttc
     3001 tggatgtgag gctatggatt cccaggccct ggtgcgctgt ctgagaggca aaagtgaagc
     3061 agagattctg gctattaaca aggtcttcaa gatgatccct gctgtggtgg atggggagtt
     3121 cttccccagg catcccaaag agttgttggc atctgaggat tttcaccctg tccccagcat
     3181 cattggtgtc aacaatgatg agtttggctg gtccattcct gtggtcatgg gctctgctca
     3241 gatgataaaa ggaataacca gagagaacct gcaggctgtt ctgaaggata cagcagtaca
     3301 aatgatgctg cctcctgagt gtagtgacct gctaatggaa gagtacatgg gggacactga
     3361 ggatgcccag accctccaaa tacagttcac agagatgatg ggagatttca tgtttgtgat
     3421 ccctgcactc caagtagcac attttcaacg ctcccatgcc cctgtctact tctatgagtt
     3481 ccaacatcca cccagctact tcaaggatgt cagaccaccc cacgtgaagg ctgaccatgc
     3541 tgatgagatt ccttttgtct ttgcgtcctt cttctggggc atgaaacttg acttcactga
     3601 ggaggaggag ctgctgagca ggaggatgat gaagtactgg gccaactttg cacgacatgg
     3661 gaaccccaac agtgagggtc taccctactg gcctgtgatg gaccatgatg agcagtacct
     3721 gcaactggac atccagcctg ctgtgggtcg agccctgaag gctggaaggc tgcagttctg
     3781 gaccaagact ctgccccaga agatccagga gctaaaggct tctcaggaca agaaaaaggg
     3841 tgggcgcgcc gacccagctt tcttgtacaa agtggttgat ctagagggcc cgcggttcga
     3901 aggtaagcct atccctaacc ctctcctcgg tctcgattct acgcgtaccg gtcatcatca
     3961 ccatcaccat tgagtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
     4021 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     4081 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     4141 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     4201 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
     4261 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     4321 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     4381 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     4441 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     4501 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     4561 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     4621 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     4681 aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     4741 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     4801 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     4861 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     4921 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct
     4981 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     5041 aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg
     5101 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
     5161 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
     5221 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
     5281 gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
     5341 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
     5401 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
     5461 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
     5521 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
     5581 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg
     5641 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
     5701 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
     5761 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
     5821 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
     5881 cttcttgacg agttcttctg agcgggactc tggggttcgc gaaatgaccg accaagcgac
     5941 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     6001 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     6061 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     6121 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     6181 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta
     6241 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     6301 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     6361 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     6421 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     6481 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     6541 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     6601 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     6661 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     6721 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     6781 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     6841 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     6901 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     6961 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     7021 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     7081 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     7141 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     7201 caagcagcag attacgcgca gaaaaaaagg atctcaa
//
