LOCUS       Exported                7228 bp DNA     circular SYN 28-MAR-2023
DEFINITION  .
ACCESSION   <unknown
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7228)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 28, 2023 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7228
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(171..1031)
                     /codon_start=1
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #ccffcc"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        1032..1136
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff"
     misc_feature    1195..1247
                     /label=bom
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=37.5886524822695"
                     /note="/other=misc feature"
                     /note="color: #a6acb3; direction: RIGHT"
     enhancer        1402..1781
                     /label=CMV enhancer
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=enhancer"
                     /note="color: #a6acb3; direction: RIGHT"
     promoter        1782..1985
                     /label=CMV promoter
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: RIGHT"
     LTR             1981..2014
                     /label=5' LTR (truncated)
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=97.059"
                     /note="/match_length=18.88888888888889"
                     /note="/other=LTR"
                     /note="color: #ffe4c4"
     promoter        2030..2048
                     /label=T7 promoter
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: RIGHT"
     protein_bind    2078..2102
                     /label=attB1
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein bind"
                     /note="color: #31849b; direction: RIGHT"
     CDS             2129..3823
                     /codon_start=1
                     
                     /note="color: #ff0000"
                     /translation="MPLARLPGWLCVVACGLLLLLQHVHGDYKDDDDKGGGQDSASPIR
                     NTHRGQVRGSFVHVKDTKSGVHAFLGIPFAKPPVGLLRFAPPEDPEPWSGVRDGTSQPA
                     MCLQPDIMNLEDAKEMNLILPPISMSEDCLYLNIYTPTHAQEGSNLPVMVWIHGGGLVV
                     GSASMNDVSKLAATEEIVIVAIQYRLGVLGFFSTGDQHARGNWGYLDQVAALRWVQKNI
                     AYFGGNRDRVTIFGVAAGGTSVSSHILSPMSKGLFHGAIMQSGVALLPDLISDTSEVVY
                     KTVANLSGCEATDSEALIHCLRAKSKQEILAINQVFKMIPAVVDGEFLPKHPQELLTSM
                     DFHPVPSIIGVNTDECGWGVPMFMGLDHIIKNITRETLPAVLKNTAARMMLPPECSHLL
                     VEEYMGDTEDPETLQAQFREMLGDFMFVIPALQVAHFQRSQAPVYFYEFQHLSSFIKHV
                     RPSHVKADHGDDVAFVFGSYLWDMNLDLTEEEELLKRMMMKYWANFARNGNPNSEGLPS
                     WPVLDHDEQYLQLDTQPAVGRALKARRLQFWTKTLPQKIQELKGSQDK"
     misc_feature    2129..2206
                     /label=Signal peptide
                     /note="color: #00ffff"
     misc_feature    2207..2239
                     /label=FLAG-linker
                     /note="color: #85dae9"
     misc_feature    2840..2842
                     /label=S227A
                     /note="color: #a6acb3"
     CDS             3755..3760
                     /codon_start=1
                     /label=RR cleavage site
                     /note="color: #3366ff"
                     /translation="RR"
     primer_bind     complement(3796..3823)
                     /label=Primer 1
                     /note="color: black; sequence: 
                     aaactacttgtcctgagaacccttgagctcctgg; added: 2021-08-24"
     protein_bind    3843..3867
                     /label=attB2
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein bind"
                     /note="color: #31849b"
     CDS             3893..3934
                     /codon_start=1
                     /label=V5 tag
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="GKPIPNPLLGLDST"
     CDS             3943..3961
                     /codon_start=1
                     /label=8xHis
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=79.16666666666666"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="SSSPSP"
     polyA_signal    3990..4214
                     /label=bGH poly(A) signal
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA signal"
                     /note="color: #a6acb3; direction: RIGHT"
     promoter        4218..4239
                     /label=SV40 promoter
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=6.197183098591549"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      4260..4688
                     /direction=RIGHT
                     /label=f1 ori
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
                     /note="color: #ffff00"
     promoter        4702..5031
                     /label=SV40 promoter
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             5098..5892
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    6068..6198
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=98.496"
                     /note="/match_length=97.08029197080292"
                     /note="/other=polyA signal"
                     /note="color: #a6acb3"
     protein_bind    6260..6282
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=95.83333333333334"
                     /note="/other=protein bind"
                     /note="color: #31849b; direction: RIGHT"
     promoter        6286..6316
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=96.875"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff"
     protein_bind    6331..6352
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein bind"
                     /note="color: #31849b; direction: RIGHT"
     CDS             complement(6365..6458)
                     /codon_start=1
                     /label=lacI
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.679593721144968"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="SAQYANRLSPRVGRFINAAGTTGFPTGKRAV"
     rep_origin      6640..7228
                     /label=ori
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
                     /note="color: #ffff00"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt
     1201 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
     1261 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
     1321 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
     1381 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
     1441 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
     1501 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
     1561 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
     1621 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
     1681 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
     1741 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
     1801 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
     1861 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
     1921 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
     1981 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
     2041 actataggga gacccaagct ggctagttaa gctatcaaca agtttgtaca aaaaagcagg
     2101 ctccgcggcc gcccccttca ccgccaccat gccattggct agacttcctg gttggctgtg
     2161 tgttgttgcc tgtgggctcc tgcttctcct ccagcatgtg catggcgact acaaggatga
     2221 cgacgataag gggggcggtc aggactcagc cagccccatc aggaacaccc acaggggtca
     2281 ggtgagaggc agctttgtcc acgtgaagga cactaaatct ggtgtccatg ccttcctggg
     2341 aatccccttt gccaagcctc ctgtaggact actgcgcttt gcacctcctg aggaccctga
     2401 gccatggagt ggtgtgagag atgggacctc acagccggcc atgtgtctgc agcctgatat
     2461 aatgaattta gaggatgcga aggaaatgaa tctgatcctg cctcccatct ctatgtccga
     2521 ggactgcctg tatctcaaca tctacacacc aacccacgcc caggagggct ctaacctgcc
     2581 tgtgatggta tggatccacg gtggtggact ggttgtagga tcagcttcca tgaatgatgt
     2641 atctaaactg gcagccactg aggaaatagt aattgtcgcc atccagtatc gcttgggggt
     2701 ccttggcttc ttcagcactg gagaccagca tgccagaggc aactggggat acctggacca
     2761 agtggctgcc ttacgctggg tgcagaagaa tattgcttac tttggaggca accgtgacag
     2821 ggtcactata tttggagtcg cagcaggagg tacaagtgtg tcttctcata ttctgtcccc
     2881 aatgtccaaa ggactctttc atggagccat tatgcagagt ggagtggccc tgctgcctga
     2941 cctcatctct gacacttctg aggtggtcta caagacagta gccaacctat ctggttgtga
     3001 agccacagac tcagaagccc tgatccactg cctgagagca aagagtaaac aagagatcct
     3061 ggctattaac caggtcttca agatgatccc tgctgtggtg gatggggagt tcctacccaa
     3121 gcatcctcag gagctgctga cctctatgga ttttcaccct gtccccagca tcattggtgt
     3181 caacacagat gaatgtggct ggggagtccc catgttcatg ggccttgacc atatcataaa
     3241 gaacataacc agagagaccc tgccagctgt tctgaagaac acagcagcac ggatgatgtt
     3301 gcctccagaa tgtagtcacc tgctagtgga ggagtatatg ggggacactg aggacccaga
     3361 gaccctgcaa gcacagttca gagagatgct gggggacttc atgtttgtga tccctgctct
     3421 ccaagtggca catttccagc gttcccaggc tcctgtctac ttctatgagt tccagcatct
     3481 gtccagcttc atcaagcatg tcaggccatc acatgtgaag gcagaccatg gtgatgacgt
     3541 tgcctttgtc tttggctcct atttgtggga catgaatttg gacctcactg aggaggagga
     3601 gctgctaaag aggatgatga tgaagtactg ggccaatttc gcaagaaatg ggaaccccaa
     3661 cagtgagggt ctaccctcct ggcctgtgtt ggaccatgat gagcagtacc tgcagctgga
     3721 cacccagcct gctgtgggtc gagccctgaa ggccagaagg ctgcagttct ggaccaagac
     3781 tctgccccag aagatccagg agctcaaggg ttctcaggac aagaaaaagg gtgggcgcgc
     3841 cgacccagct ttcttgtaca aagtggttga tctagagggc ccgcggttcg aaggtaagcc
     3901 tatccctaac cctctcctcg gtctcgattc tacgcgtacc ggtcatcatc accatcacca
     3961 ttgagtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     4021 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     4081 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     4141 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     4201 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
     4261 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     4321 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     4381 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     4441 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     4501 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     4561 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     4621 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     4681 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     4741 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     4801 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     4861 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     4921 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
     4981 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
     5041 ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg
     5101 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     5161 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     5221 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag
     5281 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     5341 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     5401 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     5461 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     5521 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     5581 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc
     5641 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     5701 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     5761 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     5821 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     5881 gagttcttct gagcgggact ctggggttcg cgaaatgacc gaccaagcga cgcccaacct
     5941 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     6001 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     6061 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     6121 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     6181 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
     6241 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     6301 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     6361 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     6421 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     6481 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     6541 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     6601 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     6661 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     6721 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     6781 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     6841 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     6901 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     6961 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     7021 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     7081 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     7141 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     7201 gattacgcgc agaaaaaaag gatctcaa
//
