LOCUS       pCA1.269_EL2_(Te        3747 bp DNA     circular SYN 28-MAR-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   SynthSys Center
  TITLE     Direct Submission
  JOURNAL   Exported Mar 29, 2023 from SnapGene 6.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3747
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     misc_feature    15..18
                     /label=GCTT
     misc_feature    47..583
                     /label=lacZ
     misc_feature    611..614
                     /label=CGCT
     misc_feature    615..618
                     /label=GGGA
     promoter        complement(1123..1246)
                     /label=P lac prom
                     /label=P\lac\prom
                     /note="/vntifkey=30"
     promoter        complement(1154)
                     /label=P(LAC)
                     /note="lac promoter"
                     /note="/vntifkey=30"
     rep_origin      1514
                     /label=ORI
                     /note="RNaseH cleavage point"
                     /note="/vntifkey=33"
     CDS             complement(2276..3133)
                     /codon_start=1
                     /label=AP(R)
                     /note="bla gene- Ap(r) determinant"
                     /note="/vntifkey=4"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     variation       2417
                     /label=G to A (BsaI removed)
                     /label=G\to\A\(BsaI\removed)
                     /label=nonstandard type:  mutation
                     /note="/vntifkey=62"
     variation       3332
                     /label=A to T (Esp3I removed)
                     /label=A\to\T\(Esp3I\removed)
                     /label=nonstandard type:  mutation
                     /note="/vntifkey=62"
     variation       3388
                     /label=G to A (Esp3I removed)
                     /label=G\to\A\(Esp3I\removed)
                     /label=nonstandard type:  mutation
                     /note="/vntifkey=62"
ORIGIN
        1 ttgaagacaa actagctttg agaccgcagc tggcacgaca ggtttgccga ctggaaagcg
       61 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
      121 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
      181 ggaaacagct atgaccatga ttacgccaag cttgcatgcc tgcaggtcga ctctagagga
      241 tccccgggta ccgagctcga attcactggc cgtcgtttta caacgtcgtg actgggaaaa
      301 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
      361 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
      421 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg
      481 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
      541 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
      601 gacggtctca cgctgggatt gtcttcgtgg aaacttggga cttcagattc caccccttgc
      661 aagcgtggaa agccttccga ccaagggaag tgtcggatgt agaggaaatg gaaagtttgt
      721 tctcagatgg ggacttgctt gattgcttca caagaatgcc agcttatgcg gtaaacgcag
      781 aggaagattt agctgcaatc aggaaaacgc ccgagatgga tgtcggtcaa gaagttaaag
      841 aacctgcagg agacagaaat caatactcaa accctgcaga aactttcctc aacaagctcc
      901 acaggaaaca cagtagggag gtgaaacacc aggccgcaaa gaaagctaaa cgcctagctg
      961 aaatccagga gtcaatgaga gctgaaggtg atgccgaacc aaatgaaata agcgggggca
     1021 tgggggcaat acccagcaac gccgaacttc ctgggcccaa aggggatcct ctagagtcga
     1081 cctgcaggca tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     1141 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     1201 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     1261 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     1321 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     1381 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     1441 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     1501 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     1561 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     1621 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     1681 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     1741 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     1801 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     1861 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     1921 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     1981 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     2041 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     2101 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     2161 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     2221 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     2281 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     2341 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     2401 caatgatacc gcgagaacca cgctcaccgg ctccagattt atcagcaata aaccagccag
     2461 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     2521 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     2581 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     2641 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     2701 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     2761 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     2821 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     2881 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     2941 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     3001 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     3061 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     3121 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     3181 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     3241 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     3301 ataaaaatag gcgtatcacg aggccctttc gactcgcgcg tttcggtgat gacggtgaaa
     3361 acctctgaca catgcagctc ccggagatgg tcacagcttg tctgtaagcg gatgccggga
     3421 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
     3481 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     3541 gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt
     3601 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
     3661 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
     3721 cggccagtga attcgagctc ggtaccc
//
