LOCUS       pC0.338_Trc10_TS        2281 bp DNA     circular SYN 11-JAN-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Alejandra Schiavon
  TITLE     Direct Submission
  JOURNAL   Exported Mar 29, 2023 from SnapGene 6.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2281
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     CDS             203..1213
                     /codon_start=1
                     /label=Sm/Sp no DraIII
                     /label=Sm/Sp\no\DraIII
                     /note="/vntifkey=4"
                     /translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQAR
                     YAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLLAV
                     HLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTI
                     VVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPA
                     AEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVA
                     ADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     variation       1180
                     /label=G to A
                     /label=G\to\A
                     /label=nonstandard type:  mutation
                     /note="/vntifkey=62"
     misc_feature    1373..1987
                     /label=rep (pMB1)
                     /label=rep\(pMB1)
                     /note="/vntifkey=21"
     rep_origin      1972
                     /label=ORI
                     /note="RNaseH cleavage point"
                     /note="/vntifkey=33"
     primer_bind     complement(2210..2226)
                     /label=Primer 2
     misc_feature    2220..2223
                     /label=GGAG
     misc_feature    2224..2253
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     primer_bind     join(2254..2281,1..4)
                     /label=Primer 1
ORIGIN
        1 tggacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
       61 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
      121 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
      181 ataatattga aaaaggaaga gtatgcgctc acgcaactgg tccagaacct tgaccgaacg
      241 cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttggggt
      301 acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat
      361 gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat
      421 catgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat
      481 cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg
      541 cggcctgaag ccacacagcg atattgattt gctggttacg gtgaccgtaa ggcttgatga
      601 aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag
      661 cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg
      721 ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg
      781 tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga
      841 acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca
      901 ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc
      961 tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg
     1021 caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta
     1081 tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc
     1141 ctcgcgcgca gatcagttgg aagaatttgt ccattacgta aaaggcgaga tcaccaaggt
     1201 agtcggcaaa taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     1261 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     1321 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     1381 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     1441 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     1501 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     1561 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     1621 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     1681 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     1741 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     1801 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     1861 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     1921 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     1981 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     2041 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     2101 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     2161 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatcactct gtggtctcag
     2221 gagttgacaa ttaatcatcc ggctcgtata atgtactaga gaccacgaag tggctcttca
     2281 g
//
