LOCUS       Exported                9284 bp DNA     circular SYN 05-APR-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pLenti4-Puro-H19 (clone G)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9284)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 5, 2023 from SnapGene 6.0.5
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..9284
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      908..1496
                     /direction=RIGHT
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
                     /note="color: #ffff00"
     protein_bind    1784..1805
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
                     /note="color: #31849b"
     promoter        1820..1850
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff"
     protein_bind    1858..1874
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
                     /note="color: #31849b"
     misc_feature    1882..1898
                     /label=M13 rev
                     /note="color: #cc99ff; direction: RIGHT"
     promoter        1919..1937
                     /label=T3 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        1965..2191
                     /label=RSV promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: RIGHT"
     LTR             2192..2372
                     /label=5' LTR (truncated)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=LTR"
                     /note="color: #ffe4c4"
     misc_feature    2419..2544
                     /label=HIV-1 Psi
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
                     /note="color: #a6acb3"
     misc_feature    3037..3270
                     /label=RRE
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
                     /note="color: #a6acb3"
     enhancer        3779..4080
                     /label=CMV enhancer
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=99.3"
                     /note="/other=enhancer"
                     /note="color: #a6acb3"
     promoter        4081..4284
                     /label=CMV promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: RIGHT"
     protein_bind    4286..4304
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    4307..4325
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     misc_feature    4338..6625
                     /label=human lncRNA H19
                     /note="color: #3366ff; direction: RIGHT"
     CDS             6632..6673
                     /codon_start=1
                     /label=V5 tag
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6683..6700
                     /label=6xHis
                     /note="color: #993366; direction: RIGHT"
     promoter        6757..7086
                     /label=SV40 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    6937..7072
                     /label=SV40 ori
                     /note="color: #ffff00"
     CDS             7096..7695
                     /codon_start=1
                     /label=PuroR
                     /label=PuroR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.3"
                     /note="/match_length=100.2"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFPATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFPETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     LTR             7767..8000
                     /label=3' LTR (Delta-U3)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=LTR"
                     /note="color: #ffe4c4"
     polyA_signal    8072..8193
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
                     /note="color: #a6acb3"
     rep_origin      8233..8368
                     /label=SV40 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
                     /note="color: #ffff00"
     promoter        complement(8389..8407)
                     /label=T7 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: LEFT"
     misc_feature    complement(8417..8433)
                     /label=M13 fwd
                     /note="color: #cc99ff; direction: LEFT"
     rep_origin      8575..9003
                     /direction=RIGHT
                     /label=f1 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
                     /note="color: #ffff00"
     promoter        9056..9160
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             join(9161..9284,1..737)
                     /codon_start=1
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #ccffcc"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
       61 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
      121 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
      181 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
      241 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
      301 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
      361 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
      421 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
      481 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
      541 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
      601 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
      661 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
      721 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
      781 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
      841 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
      901 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
      961 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     1021 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     1081 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     1141 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     1201 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     1261 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     1321 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     1381 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     1441 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     1501 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     1561 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     1621 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     1681 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     1741 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     1801 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     1861 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
     1921 ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat
     1981 actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa
     2041 aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag
     2101 gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat
     2161 tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc
     2221 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     2281 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     2341 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
     2401 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
     2461 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
     2521 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
     2581 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
     2641 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
     2701 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     2761 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     2821 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
     2881 gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     2941 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     3001 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     3061 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     3121 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     3181 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     3241 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     3301 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     3361 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     3421 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     3481 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     3541 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     3601 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     3661 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
     3721 cagatccatt cgattagtga acggatctcg acggtatcga taagcttggg agttccgctt
     3781 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     3841 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     3901 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     3961 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     4021 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     4081 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
     4141 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
     4201 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
     4261 tgggaggtct atataagcag agctctccct atcagtgata gagatctccc tatcagtgat
     4321 agagatcgtc gactagtggg gtaacggggg aaactgggga agtggggaac cgaggggcaa
     4381 ccaggggaag atggggtgct ggaggagagc ttgtgggagc caaggagcac cttggacatc
     4441 tggagtctgg caggagtgat gacgggtgga ggggctagct cgaggcaggg ctggtggggc
     4501 ctgaggccag tgaggagtgt ggagtaggtg cccaggcatc gtgcagacag ggcgacatca
     4561 gctggggacg atgggcctga gctagggctg gaaagaaggg ggagccaggc attcatcccg
     4621 gtcacttttg gttacaggac gtggcagctg gttggacgag gggagctggt gggcagggtt
     4681 tgatcccagg gcctgggcaa cggaggtgta gctggcagca gcgggcaggt gaggacccca
     4741 tctgccgggc aggtgagtcc cttccctccc caggcctcgc ttccccagcc ttctgaaaga
     4801 aggaggttta ggggatcgag ggctggcggg gagaagcaga caccctccca gcagaggggc
     4861 aggatggggg caggagagtt agcaaaggtg acatcttctc ggggggagcc gagactgcgc
     4921 aaggctgggg ggctatgggc ccgttccggg cagaaagagc aagagggcag ggagggagca
     4981 caggggtggc cagcgtaggg tccagcacgt ggggtggtac cccaggcctg ggtcagacag
     5041 ggacaaggca ggggacacag gacagagggg tccccagctg ccacctcacc caccgcaatt
     5101 catttagtag caggcacagg ggcagctccg gcacggcttt ctcaggccta tgccggagcc
     5161 tcgagggctg gagagcggga agacaggcag tgctcgggga gttgcagcag gacatcacca
     5221 ggagggcgaa gcggccacgg gaggggggcc ccgggacatt gcgcagcaag gaggctgcag
     5281 gggctcggcc tgcgggcgcc ggtcccacga ggcactgcgg cccagggtct ggtgcggaga
     5341 gggcccacag tggacttggt gacgctgtat gccctcaccg ctcagcccct ggggctggct
     5401 tggcagacag tacagcatcc gggggagtca agggcatggg gcgagaccag actaggcgag
     5461 gcgggcgggg cggagtgaat gagctctcag gagggaggat ggtgcaggca ggggtgagga
     5521 gcgcagcggg cggcgagcgg gaggcactgg cctccagagc ccgtggccaa ggcgggcctc
     5581 gcgggcggcg acggagccgg gatcggtgcc tcagcgttcg ggctggagac gaggccaggt
     5641 ctccagctgg ggtggacgtg cccaccagct gccgaaggcc aagacgccag gtccggtgga
     5701 cgtgacaagc aggacatgac atggtccggt gtgacggcga ggacagagga ggcgcgtccg
     5761 gccttcctga acaccttagg ctggtggggc tgcggcaaga agcgggtctg tttctttact
     5821 tcctccacgg agtcggcaca ctatggctgc cctctgggct cccagaaccc acaacatgaa
     5881 agaaatggtg ctacccagct caagcctggg cctttgaatc cggacacaaa accctctagc
     5941 ttggaaatga atatgctgca ctttacaacc actgcactac ctgactcagg aatcggctct
     6001 cgaaggtgaa gcgagaggaa ccagacctca tcagcccaac atcaaagaca ccatcggaac
     6061 agcagcgccc gcagcaccca ccccgcaccg gcgactccat cttcacggcc accccctgcg
     6121 gcggacggtt gaccaccagc caccacatca tcccagagct gagctcctcc agcgggatga
     6181 cgccgtcccc accacctccc tcttcttctt tttcatcctt ctgtctcttt gtttctgagc
     6241 tttcctgtct ttcctttttt tctgagagat tcaaagcctc cacgactctg tttcccccgt
     6301 cccttctgaa tttaatttgc actaagtcat ttgcactggt tggagttgtg gagacggcct
     6361 tgagtctcgg tgcgagtgtg cgtgagtgtg agccaccttg gcaagtgcct gcgcagggcc
     6421 cggccgccct ccatctgggc cgggtgactg ggcgccggct gtgtgcccga ggcctcaccc
     6481 tgccctcgcc tagtctggaa gctccgaccg acatcacgga gcagccttca agcattccat
     6541 tacgccccat ctcgctctgt gcccctcccc accagggctt cagcaggagc cctggactca
     6601 tcatcaataa acactgttac agcaattcga aggtaagcct atccctaacc ctctcctcgg
     6661 tctcgattct acgcgtaccg gtcatcatca ccatcaccat tgagtttaaa cctgctgatc
     6721 agcctcgact gtgccttcta gtcgaccctg tggaatgtgt gtcagttagg gtgtggaaag
     6781 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     6841 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     6901 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     6961 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     7021 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     7081 tgcaaaaagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     7141 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     7201 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     7261 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     7321 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     7381 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     7441 caccggccca aggagcccgc gtggttcccg gccaccgtcg gcgtctcgcc cgaccaccag
     7501 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     7561 gtgcccgcct tcccggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     7621 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     7681 aagcccggtg cctgaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     7741 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     7801 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     7861 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     7921 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     7981 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     8041 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     8101 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     8161 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
     8221 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     8281 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     8341 aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt
     8401 cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     8461 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     8521 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc
     8581 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     8641 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     8701 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     8761 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     8821 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     8881 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     8941 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     9001 attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg
     9061 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     9121 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     9181 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     9241 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgag
//
