LOCUS       pEG302-TRB2-ZF     14229 bp    DNA     circular UNA 10-FEB-2021
DEFINITION  an expression clone created from
            pEarleyGate302_GW_3FLAG_ZF108_BirA_BASTA and pENTR-RING1 CDS no
            stop codon.
ACCESSION   urn.local...22-czziff3
VERSION     urn.local...22-czziff3
KEYWORDS    .
FEATURES             Location/Qualifiers
     misc_feature    3..27
                     /note="left border repeat from nopaline C58 T-DNA"
                     /label="LB T-DNA repeat"
     terminator      113..365
                     /note="mannopine synthase terminator"
                     /label="MAS terminator"
     CDS             complement(375..926)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /note="confers resistance to bialophos or
                     phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQ
                     EWTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGL
                     GSTLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHD
                     VGFWQLDFSLPVPPRPVLPVTEI"
                     /label="Streptomyces hygroscopicus bar CDS"
     promoter        complement(932..1312)
                     /note="mannopine synthase promoter (Velten et al., 1984)"
                     /label="MAS promoter"
     TRB2gDNA        1409..3547
     CDS             3647..3718
                     /created_by="User"
                     /label="3xFlag"
     CDS             3749..4254
                     /created_by="User"
                     /label="ZF108 (not sure about exact boundary)"
     CDS             4256..4342
                     /created_by="User"
                     /label="BLRP (not sure about exact boundary)"
     terminator      4398..5105
                     /note="octopine synthase terminator"
                     /label="OCS terminator"
     TRB1gDNA        5117..6565
                     /label="Actin promoter"
     CDS             6566..7531
                     /created_by="User"
                     /label="BirA gene"
     primer_bind     7423..7443
                     /created_by="primer3"
                     /%GC="47.62"
                     /Tm="54.58"
                     /Hairpin="6.0"
                     /Primer-Dimer="2.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Sequence="TGGCATTTCACGCGGAATAGA"
                     /annotation_group="PrimerPluginGroup25_urn:local:cat:15478
                     73576597.47"
                     /label="18991"
     misc_feature    7950..7974
                     /note="right border repeat from nopaline C58 T-DNA"
                     /label="RB T-DNA repeat"
     CDS             9275..9904
                     /codon_start=1
                     /product="stability protein from Pseudomonas plasmid pVS1"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSA
                     RDWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVL
                     IPVQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYE
                     LPILESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
                     /label="stability protein from Pseudomonas plasmid
                     pVS1CDS"
     CDS             10333..11406
                     /codon_start=1
                     /product="replication protein from Pseudomonas plasmid
                     pVS1"
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGES
                     WQAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFR
                     LSKRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKG
                     DKPGRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTV
                     SEGEALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIG
                     RFLYRLARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEA
                     GQAGPILVMRYRNLIEGEASAGS"
                     /label="replication protein from Pseudomonas plasmid
                     pVS1CDS"
     rep_origin      11472..11666
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /label="pVS1 oriV"
     misc_feature    12010..12150
                     /note="basis of mobility region from pBR322"
                     /label="bom"
     rep_origin      complement(12336..12924)
                     /direction="LEFT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(13011..13805)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
                     /label="aphA-3 CDS"
ORIGIN
        1 GCTGGCAGGA TATATTGTGG TGTAAACAAA TTGACGCTTA GACAACTTAA TAACACATTG
       61 CGGACGTTTT TAATGTACTG AATTAACGCC GAATTAATTC GAGCTCGGAT CTGATAATTT
      121 ATTTGAAAAT TCATAAGAAA AGCAAACGTT ACATGAATTG ATGAAACAAT ACAAAGACAG
      181 ATAAAGCCAC GCACATTTAG GATATTGGCC GAGATTACTG AATATTGAGT AAGATCACGG
      241 AATTTCTGAC AGGAGCATGT CTTCAATTCA GCCCAAATGG CAGTTGAAAT ACTCAAACCG
      301 CCCCATATGC AGGAGCGGAT CATTCATTGT TTGTTTGGTT GCCTTTGCCA ACATGGGAGT
      361 CCAAGATTCT GCAGTCAAAT CTCGGTGACG GGCAGGACCG GACGGGGCGG TACCGGCAGG
      421 CTGAAGTCCA GCTGCCAGAA ACCCACGTCA TGCCAGTTCC CGTGCTTGAA GCCGGCCGCC
      481 CGCAGCATGC CGCGGGGGGC ATATCCGAGC GCCTCGTGCA TGCGCACGCT CGGGTCGTTG
      541 GGCAGCCCGA TGACAGCGAC CACGCTCTTG AAGCCCTGTG CCTCCAGGGA CTTCAGCAGG
      601 TGGGTGTAGA GCGTGGAGCC CAGTCCCGTC CGCTGGTGGC GGGGGGAGAC GTACACGGTC
      661 GACTCGGCCG TCCAGTCGTA GGCGTTGCGT GCCTTCCAGG GGCCCGCGTA GGCGATGCCG
      721 GCGACCTCGC CGTCCACCTC GGCGACGAGC CAGGGATAGC GCTCCCGCAG ACGGACGAGG
      781 TCGTCCGTCC ACTCCTGCGG TTCCTGCGGC TCGGTACGGA AGTTGACCGT GCTTGTCTCG
      841 ATGTAGTGGT TGACGATGGT GCAGACCGCC GGCATGTCCG CCTCGGTGGC ACGGCGGATG
      901 TCGGCCGGGC GTCGTTCTGG GCTCATCGAT TCGATTTGGT GTATCGAGAT TGGTTATGAA
      961 ATTCAGATGC TAGTGTAATG TATTGGTAAT TTGGGAAGAT ATAATAGGAA GCAAGGCTAT
     1021 TTATCCATTT CTGAAAAGGC GAAATGGCGT CACCGCGAGC GTCACGCGCA TTCCGTTCTT
     1081 GCTGTAAAGC GTTGTTTGGT ACACTTTTGA CTAGCGAGGC TTGGCGTGTC AGCGTATCTA
     1141 TTCAAAAGTC GTTAATGGCT GCGGATCAAG AAAAAGTTGG AATAGAAACA GAATACCCGC
     1201 GAAATTCAGG CCCGGTTGCC ATGTCCTACA CGCCGAAATA AACGACCAAA TTAGTAGAAA
     1261 AATAAAAACT GACTCGGATA CTTACGTCAC GTCTTGCGCA CTGATTTGAA AAATCTCAGA
     1321 ATTGAATTCT GCAGTCGACG GTACCGCGGG CCCGGGATCA TCAACAAGTT TGTACAAAAA
     1381 AGCAGGCTCC GCGGCCGCCC CCTTCACCaa cacatatttc aatctagcca tcaaaatttt
     1441 ataaattacg ctttcggtct tactattata tgaaaaatct ttaatagccc ctaaactatc
     1501 tgtgagattt aacaacgata aattttatag atacattatt tttaaaaaat agtactagta
     1561 tattttattc agcttgatcc tattcattta gttggtaggt tttattgtgt aatttgataa
     1621 acttgaggca aatttgtgaa tgtggagaaa tatagggtta catcttcctt agcttgaata
     1681 caaaagaaaa gctcaagttt gtttctcgtt tctatggata ttcttcttcc ttatgctagc
     1741 ctctctggtc ttcttcttct tcgttcgttt cagcgaatca catttgatct atgacacatc
     1801 tatacaagga attagctttc atcatcaagt ctctctctcg aaaactctcc tttggtttcg
     1861 tttgatctgg catgcttctt ctcctccttc ctcctttctg cttccttctc tgaattttcc
     1921 atttttgttt ttaattttct acgatcgccc catttgaatt tggttgataa tttagctatc
     1981 gctttagatt gatggctgta tttccaaatt cctgttgatt tgcgacgttg ttaatgggct
     2041 ttgatcctct ttttttggtt cgagattgct cgaatcactc atttttttgt tgaattgagc
     2101 tttggggcgt atctaatttc tttggatttg tgtagttaca tttttatgtt gtggctttgt
     2161 gtatctgctg gatttagatg accaaaattt tgtattggat aagaaatgtt caacaatggc
     2221 tttgatgtgg ttaagtttcg tacgatttaa cagattctaa tgttagcgtt cacatctatt
     2281 ggcagagaaa agaagaaatt caaatattga taactgtggA TGGGTGCACC AAAGCAGAAG
     2341 TGGACACCGG AAGAAGAAGC AGCTCTTAAA GCTGGAGTTC TTAAGCATGG GACTGGGAAG
     2401 TGGCGTACCA TACTCTCGGA TACTGAGTTT AGTTTAATCC TTAAGTCTCG CTCTAATGTT
     2461 GATCTTAAGg taatacaata taatatgctt tttggaggtt gtgatcgcgt gtttcggact
     2521 aatcttgata gttcaaggca taacagGACA AATGGAGAAA TATAAGCGTG ACAGCTTTAT
     2581 GGGGTTCAAG GAAGAAGGCT AAACTTGCGC TTAAGAGGAC TCCACCAGGT ACTAAACAGG
     2641 ATGATAACAA CACAGCTCTT ACCATTGTGG CTTTGACTAA TGATGATGAA CGTGCAAAAC
     2701 CAACTTCCCC CGGAGGTTCT GGTGGTGGAT CACCACGCAC TTGTGCTTCT AAGAGATCAA
     2761 TTACAAGgtt tggttccctg acttatgcac cacataacga ttttcttgag ttcataaaga
     2821 tttgatcttg cgctattgtc tgtttttgta gTTTGGATAA GATCATCTTT GAGGCAATTA
     2881 CCAACTTGAG GGAACTACGC GGTTCTGACA GAACATCAAT CTTTCTGTAT ATAGAGgtat
     2941 ttctaccatt gggattacat gtttggttgg ggtttgttgc atacttactt agtagctgtt
     3001 aaatttcagG AGAATTTCAA GACTCCACCG AATATGAAAA GGCATGTCGC AGTACGATTA
     3061 AAGCATTTAT CATCGAATGG AACATTAGTT AAGgtaaaca cacaactttt gattcaagat
     3121 tcatgatcac taacacttca ttactctcag aattttcaaa aatacttact tggtgatttc
     3181 tcactttcag ATAAAGCACA AGTACAGGTT TTCTTCTAAT TTTATTCCCG CAGGTGCAAG
     3241 ACAGAAGGCT CCTCAACTCT TTCTGGAAGG AAACAACAAG AAAGACCCAA CAAAACCCGA
     3301 GGAGAACGGT GCCAACAGTC TCACTAAATT CCGAGTAGAC GGTGAATTAT ATATGATAAA
     3361 AGGCATGACA GCTCAAGAAG CTGCAGAGGC TGCAGCAAGA GCAGTTGCAG AGGCAGAATT
     3421 TGCTATCACA GAGGCTGAAC AAGCAGCAAA AGAGGCAGAA AGAGCAGAAG CAGAAGCTGA
     3481 AGCTGCTCAG ATATTTGCAA AGGCAGCCAT GAAAGCTTTG AAGTTCAGGA TCCGTAATCA
     3541 TCCTTGGAAG GGTGGGCGCG CCGACCCAGC TTTCTTGTAC AAAGTGGTGC TAGGTGAGTC
     3601 TAGAGAGTTA ATTAAGACCC GGGACGGGCG CGCCAAGCTT GGTACCGACT ACAAAGACGA
     3661 TGACGACAAA GACTACAAAG ACGATGACGA CAAAGACTAC AAAGACGATG ACGACAAAGG
     3721 ATCCCTTGAG GTTCTTTTCC AAGGACCTCT CGAGCCTTAT AAATGCCCTG AGTGTGGAAA
     3781 ATCTTTCTCT CAATCTGGAG ATCTTAGGAG GCACCAAAGG ACCCATACCG GTGAAAAACC
     3841 TTATAAATGC CCTGAGTGTG GAAAATCATT CAGTAGGAGT GATCATCTTA CCACCCATCA
     3901 GAGGACCCAT ACTGGTGAAA AACCTTATAA ATGCCCTGAG TGTGGAAAAT CATTCTCACA
     3961 ATCTTCATCT CTTGTTAGGC ATCAGAGGAC TCATACCGGA GAAAAACCTT ATAAATGCCC
     4021 TGAGTGTGGA AAATCATTCT CAACATCTGG AAACCTTGTT AGGCACCAAA GGACCCATAC
     4081 TGGTGAAAAA CCTTATAAAT GCCCTGAGTG TGGAAAATCA TTCTCTCAGA GAGCAAACCT
     4141 TAGGGCTCAT CAAAGGACAC ACACTGGAGA AAAACCTTAT AAATGCCCTG AGTGTGGAAA
     4201 ATCTTTCTCT AGGAGTGATA AGCTCACCGA GCATCAAAGG ACTCACACCG GACTCGAGGG
     4261 ATCTATGGCT GGTGGACTTA ACGATATCTT CGAAGCTCAG AAGATTGAAT GGCATGAGGA
     4321 TACTGGTGGA TCTTAGTAGT AGAAGCTTGG CGCGCCTCTA GAGAGTTAAT TAAGACCCGG
     4381 GACTAGTCCC TAGAGTCCTG CTTTAATGAG ATATGCGAGA CGCCTATGAT CGCATGATAT
     4441 TTGCTTTCAA TTCTGTTGTG CACGTTGTAA AAAACCTGAG CATGTGTAGC TCAGATCCTT
     4501 ACCGCCGGTT TCGGTTCATT CTAATGAATA TATCACCCGT TACTATCGTA TTTTTATGAA
     4561 TAATATTCTC CGTTCAATTT ACTGATTGTA CCCTACTACT TATATGTACA ATATTAAAAT
     4621 GAAAACAATA TATTGTGCTG AATAGGTTTA TAGCGACATC TATGATAGAG CGCCACAATA
     4681 ACAAACAATT GCGTTTTATT ATTACAAATC CAATTTTAAA AAAAGCGGCA GAACCGGTCA
     4741 AACCTAAAAG ACTGATTACA TAAATCTTAT TCAAATTTCA AAAGTGCCCC AGGGGCTAGT
     4801 ATCTACGACA CACCGAGCGG CGAACTAATA ACGCTCACTG AAGGGAACTC CGGTTCCCCG
     4861 CCGGCGCGCA TGGGTGAGAT TCCTTGAAGT TGAGTATTGG CCGTCCGCTC TACCGAAAGT
     4921 TACGGGCACC ATTCAACCCG GTCCAGCACG GCGGCCGGGT AACCGACTTG CTGCCCCGAG
     4981 AATTATGCAG CATTTTTTTG GTGTATGTGG GCCCCAAATG AAGTGCAGGT CAAACCTTGA
     5041 CAGTGACGAC AAATCGTTGG GCGGGTCCAG GGCGAATTTT GCGACAACAT GTCGAGGCTC
     5101 AGCAGGACCT GCAGGGACAA AATTTAGAAC GAACTTAATT ATGATCTCAA ATACATTGAT
     5161 ACATATCTCA TCTAGATCTA GGTTATCATT ATGTAAGAAA GTTTTGACGA ATATGGCACG
     5221 ACAAAATGGC TAGACTCGAT GTAATTGGTA TCTCAACTCA ACATTATACT TATACCAAAC
     5281 ATTAGTTAGA CAAAATTTAA ACAACTATTT TTTATGTATG CAAGAGTCAG CATATGTATA
     5341 ATTGATTCAG AATCGTTTTG ACGAGTTCGG ATGTAGTAGT AGCCATTATT TAATGTACAT
     5401 ACTAATCGTG AATAGTGAAT ATGATGAAAC ATTGTATCTT ATTGTATAAA TATCCATAAA
     5461 CACATCATGA AAGACACTTT CTTTCACGGT CTGAATTAAT TATGATACAA TTCTAATAGA
     5521 AAACGAATTA AATTACGTTG AATTGTATGA AATCTAATTG AACAAGCCAA CCACGACGAC
     5581 GACTAACGTT GCCTGGATTG ACTCGGTTTA AGTTAACCAC TAAAAAAACG GAGCTGTCAT
     5641 GTAACACGCG GATCGAGCAG GTCACAGTCA TGAAGCCATC AAAGCAAAAG AACTAATCCA
     5701 AGGGCTGAGA TGATTAATTA GTTTAAAAAT TAGTTAACAC GAGGGAAAAG GCTGTCTGAC
     5761 AGCCAGGTCA CGTTATCTTT ACCTGTGGTC GAAATGATTC GTGTCTGTCG ATTTTAATTA
     5821 TTTTTTTGAA AGGCCGAAAA TAAAGTTGTA AGAGATAAAC CCGCCTATAT AAATTCATAT
     5881 ATTTTCCTCT CCGCTTTGAA TTGTCTCGTT GTCCTCCTCA CTTTCATCAG CCGTTTTGAA
     5941 TCTCCGGCGA CTTGACAGAG AAGAACAAGG AAGAAGACTA AGAGAGAAAG TAAGAGATAA
     6001 TCCAGGAGAT TCATTCTCCG TTTTGAATCT TCCTCAATCT CATCTTCTTC CGCTCTTTCT
     6061 TTCCAAGGTA ATAGGAACTT TCTGGATCTA CTTTATTTGC TGGATCTCGA TCTTGTTTTC
     6121 TCAATTTCCT TGAGATCTGG ATATCGTTTA ATTTGGATCT GTGAACCTCC ACTAAATCTT
     6181 TTGGTTTTAC TAGAATCGAT CTAAGTTGAC CGATCAGTTA GCTCGATTAT AGCTACCAGA
     6241 ATTTGGCTTG ACCTTGATGG AGAGATCCAT GTTCATGTTA CCTGGGAAAT GATTTGTATA
     6301 TGTGAATTGA AATCTGAACT GTTGAAGTTA GATTGAATCT GAACACTGTC AATGTTAGAT
     6361 TGAATCTGAA CACTGTTTAA GGTTAGATGA AGTTTGTGTA TAGATTCTTC GAAACTTTAG
     6421 GATTTGTAGT GTCGTACGTT GAACAGAAAG CTATTTCTGA TTCAATCAGG GTTTATTTGA
     6481 CTGTATTGAA CTCTTTTTGT GTGTTTGCAG CTCATAAAAA ATGGCTGAGG CTGATGATAT
     6541 TCAACCAATC GTGTGTGACC TCGAGATGAA GGATAACACC GTGCCACTGA AATTGATTGC
     6601 CCTGTTAGCG AACGGTGAAT TTCACTCTGG CGAGCAGTTG GGTGAAACGC TGGGAATGAG
     6661 CCGGGCGGCT ATTAATAAAC ACATTCAGAC ACTGCGTGAC TGGGGCGTTG ATGTCTTTAC
     6721 CGTTCCGGGT AAAGGATACA GCCTGCCTGA GCCTATCCAG TTACTTAATG CTAAACAGAT
     6781 ATTGGGTCAG CTGGATGGCG GTAGTGTAGC CGTGCTGCCA GTGATTGACT CCACGAATCA
     6841 GTACCTTCTT GATCGTATCG GAGAGCTTAA ATCGGGCGAT GCTTGCATTG CAGAATACCA
     6901 GCAGGCTGGC CGTGGTCGCC GGGGTCGGAA ATGGTTTTCG CCTTTTGGCG CAAACTTATA
     6961 TTTGTCGATG TTCTGGCGTC TGGAACAAGG CCCGGCGGCG GCGATTGGTT TAAGTCTGGT
     7021 TATCGGTATC GTGATGGCGG AAGTATTACG CAAGCTGGGT GCAGATAAAG TTCGTGTTAA
     7081 ATGGCCTAAT GACCTCTATC TGCAGGATCG CAAGCTGGCA GGCATTCTGG TGGAGCTGAC
     7141 TGGCAAAACT GGCGATGCGG CGCAAATAGT CATTGGAGCC GGGATCAACA TGGCAATGCG
     7201 CCGTGTTGAA GAGAGTGTCG TTAATCAGGG GTGGATCACG CTGCAGGAAG CGGGGATCAA
     7261 TCTCGATCGT AATACGTTGG CGGCCATGCT AATACGTGAA TTACGTGCTG CGTTGGAACT
     7321 CTTCGAACAA GAAGGATTGG CACCTTATCT GTCGCGCTGG GAAAAGCTGG ATAATTTTAT
     7381 TAATCGCCCA GTGAAACTTA TCATTGGTGA TAAAGAAATA TTTGGCATTT CACGCGGAAT
     7441 AGACAAACAG GGGGCTTTAT TACTTGAGCA GGATGGAATA ATAAAACCCT GGATGGGCGG
     7501 TGAAATATCC CTGCGTAGTG CAGAAAAATA AGCTTTCTCC ATAATAATGT GTGAGTAGTT
     7561 CCCAGATAAG GGAATTAGGG TTCCTATAGG GTTTCGCTCA TGTGTTGAGC ATATAAGAAA
     7621 CCCTTAGTAT GTATTTGTAT TTGTAAAATA CTTCTATCAA TAAAATTTCT AATTCCTAAA
     7681 ACCAAAATCC AGTACTAAAA TCCAGATCCC TGCAGGCATG CAAGCTTGGC ACTGGCCGTC
     7741 GTTTTACAAC GTCGTGACTG GGAAAACCCT GGCGTTACCC AACTTAATCG CCTTGCAGCA
     7801 CATCCCCCTT TCGCCAGCTG GCGTAATAGC GAAGAGGCCC GCACCGATCG CCCTTCCCAA
     7861 CAGTTGCGCA GCCTGAATGG CGAATGCTAG AGCAGCTTGA GCTTGGATCA GATTGTCGTT
     7921 TCCCGCCTTC AGTTTAAACT ATCAGTGTTT GACAGGATAT ATTGGCGGGT AAACCTAAGA
     7981 GAAAAGAGCG TTTATTAGAA TAATCGGATA TTTAAAAGGG CGTGAAAAGG TTTATCCGTT
     8041 CGTCCATTTG TATGTGCATG CCAACCACAG GGTTCCCCTC GGGATCAAAG TACTTTGATC
     8101 CAACCCCTCC GCTGCTATAG TGCAGTCGGC TTCTGACGTT CAGTGCAGCC GTCTTCTGAA
     8161 AACGACATGT CGCACAAGTC CTAAGTTACG CGACAGGCTG CCGCCCTGCC CTTTTCCTGG
     8221 CGTTTTCTTG TCGCGTGTTT TAGTCGCATA AAGTAGAATA CTTGCGACTA GAACCGGAGA
     8281 CATTACGCCA TGAACAAGAG CGCCGCCGCT GGCCTGCTGG GCTATGCCCG CGTCAGCACC
     8341 GACGACCAGG ACTTGACCAA CCAACGGGCC GAACTGCACG CGGCCGGCTG CACCAAGCTG
     8401 TTTTCCGAGA AGATCACCGG CACCAGGCGC GACCGCCCGG AGCTGGCCAG GATGCTTGAC
     8461 CACCTACGCC CTGGCGACGT TGTGACAGTG ACCAGGCTAG ACCGCCTGGC CCGCAGCACC
     8521 CGCGACCTAC TGGACATTGC CGAGCGCATC CAGGAGGCCG GCGCGGGCCT GCGTAGCCTG
     8581 GCAGAGCCGT GGGCCGACAC CACCACGCCG GCCGGCCGCA TGGTGTTGAC CGTGTTCGCC
     8641 GGCATTGCCG AGTTCGAGCG TTCCCTAATC ATCGACCGCA CCCGGAGCGG GCGCGAGGCC
     8701 GCCAAGGCCC GAGGCGTGAA GTTTGGCCCC CGCCCTACCC TCACCCCGGC ACAGATCGCG
     8761 CACGCCCGCG AGCTGATCGA CCAGGAAGGC CGCACCGTGA AAGAGGCGGC TGCACTGCTT
     8821 GGCGTGCATC GCTCGACCCT GTACCGCGCA CTTGAGCGCA GCGAGGAAGT GACGCCCACC
     8881 GAGGCCAGGC GGCGCGGTGC CTTCCGTGAG GACGCATTGA CCGAGGCCGA CGCCCTGGCG
     8941 GCCGCCGAGA ATGAACGCCA AGAGGAACAA GCATGAAACC GCACCAGGAC GGCCAGGACG
     9001 AACCGTTTTT CATTACCGAA GAGATCGAGG CGGAGATGAT CGCGGCCGGG TACGTGTTCG
     9061 AGCCGCCCGC GCACGTCTCA ACCGTGCGGC TGCATGAAAT CCTGGCCGGT TTGTCTGATG
     9121 CCAAGCTGGC GGCCTGGCCG GCCAGCTTGG CCGCTGAAGA AACCGAGCGC CGCCGTCTAA
     9181 AAAGGTGATG TGTATTTGAG TAAAACAGCT TGCGTCATGC GGTCGCTGCG TATATGATGC
     9241 GATGAGTAAA TAAACAAATA CGCAAGGGGA ACGCATGAAG GTTATCGCTG TACTTAACCA
     9301 GAAAGGCGGG TCAGGCAAGA CGACCATCGC AACCCATCTA GCCCGCGCCC TGCAACTCGC
     9361 CGGGGCCGAT GTTCTGTTAG TCGATTCCGA TCCCCAGGGC AGTGCCCGCG ATTGGGCGGC
     9421 CGTGCGGGAA GATCAACCGC TAACCGTTGT CGGCATCGAC CGCCCGACGA TTGACCGCGA
     9481 CGTGAAGGCC ATCGGCCGGC GCGACTTCGT AGTGATCGAC GGAGCGCCCC AGGCGGCGGA
     9541 CTTGGCTGTG TCCGCGATCA AGGCAGCCGA CTTCGTGCTG ATTCCGGTGC AGCCAAGCCC
     9601 TTACGACATA TGGGCCACCG CCGACCTGGT GGAGCTGGTT AAGCAGCGCA TTGAGGTCAC
     9661 GGATGGAAGG CTACAAGCGG CCTTTGTCGT GTCGCGGGCG ATCAAAGGCA CGCGCATCGG
     9721 CGGTGAGGTT GCCGAGGCGC TGGCCGGGTA CGAGCTGCCC ATTCTTGAGT CCCGTATCAC
     9781 GCAGCGCGTG AGCTACCCAG GCACTGCCGC CGCCGGCACA ACCGTTCTTG AATCAGAACC
     9841 CGAGGGCGAC GCTGCCCGCG AGGTCCAGGC GCTGGCCGCT GAAATTAAAT CAAAACTCAT
     9901 TTGAGTTAAT GAGGTAAAGA GAAAATGAGC AAAAGCACAA ACACGCTAAG TGCCGGCCGT
     9961 CCGAGCGCAC GCAGCAGCAA GGCTGCAACG TTGGCCAGCC TGGCAGACAC GCCAGCCATG
    10021 AAGCGGGTCA ACTTTCAGTT GCCGGCGGAG GATCACACCA AGCTGAAGAT GTACGCGGTA
    10081 CGCCAAGGCA AGACCATTAC CGAGCTGCTA TCTGAATACA TCGCGCAGCT ACCAGAGTAA
    10141 ATGAGCAAAT GAATAAATGA GTAGATGAAT TTTAGCGGCT AAAGGAGGCG GCATGGAAAA
    10201 TCAAGAACAA CCAGGCACCG ACGCCGTGGA ATGCCCCATG TGTGGAGGAA CGGGCGGTTG
    10261 GCCAGGCGTA AGCGGCTGGG TTGCCTGCCG GCCCTGCAAT GGCACTGGAA CCCCCAAGCC
    10321 CGAGGAATCG GCGTGAGCGG TCGCAAACCA TCCGGCCCGG TACAAATCGG CGCGGCGCTG
    10381 GGTGATGACC TGGTGGAGAA GTTGAAGGCC GCGCAGGCCG CCCAGCGGCA ACGCATCGAG
    10441 GCAGAAGCAC GCCCCGGTGA ATCGTGGCAA GCGGCCGCTG ATCGAATCCG CAAAGAATCC
    10501 CGGCAACCGC CGGCAGCCGG TGCGCCGTCG ATTAGGAAGC CGCCCAAGGG CGACGAGCAA
    10561 CCAGATTTTT TCGTTCCGAT GCTCTATGAC GTGGGCACCC GCGATAGTCG CAGCATCATG
    10621 GACGTGGCCG TTTTCCGTCT GTCGAAGCGT GACCGACGAG CTGGCGAGGT GATCCGCTAC
    10681 GAGCTTCCAG ACGGGCACGT AGAGGTTTCC GCAGGGCCGG CCGGCATGGC CAGTGTGTGG
    10741 GATTACGACC TGGTACTGAT GGCGGTTTCC CATCTAACCG AATCCATGAA CCGATACCGG
    10801 GAAGGGAAGG GAGACAAGCC CGGCCGCGTG TTCCGTCCAC ACGTTGCGGA CGTACTCAAG
    10861 TTCTGCCGGC GAGCCGATGG CGGAAAGCAG AAAGACGACC TGGTAGAAAC CTGCATTCGG
    10921 TTAAACACCA CGCACGTTGC CATGCAGCGT ACGAAGAAGG CCAAGAACGG CCGCCTGGTG
    10981 ACGGTATCCG AGGGTGAAGC CTTGATTAGC CGCTACAAGA TCGTAAAGAG CGAAACCGGG
    11041 CGGCCGGAGT ACATCGAGAT CGAGCTAGCT GATTGGATGT ACCGCGAGAT CACAGAAGGC
    11101 AAGAACCCGG ACGTGCTGAC GGTTCACCCC GATTACTTTT TGATCGATCC CGGCATCGGC
    11161 CGTTTTCTCT ACCGCCTGGC ACGCCGCGCC GCAGGCAAGG CAGAAGCCAG ATGGTTGTTC
    11221 AAGACGATCT ACGAACGCAG TGGCAGCGCC GGAGAGTTCA AGAAGTTCTG TTTCACCGTG
    11281 CGCAAGCTGA TCGGGTCAAA TGACCTGCCG GAGTACGATT TGAAGGAGGA GGCGGGGCAG
    11341 GCTGGCCCGA TCCTAGTCAT GCGCTACCGC AACCTGATCG AGGGCGAAGC ATCCGCCGGT
    11401 TCCTAATGTA CGGAGCAGAT GCTAGGGCAA ATTGCCCTAG CAGGGGAAAA AGGTCGAAAA
    11461 GGTCTCTTTC CTGTGGATAG CACGTACATT GGGAACCCAA AGCCGTACAT TGGGAACCGG
    11521 AACCCGTACA TTGGGAACCC AAAGCCGTAC ATTGGGAACC GGTCACACAT GTAAGTGACT
    11581 GATATAAAAG AGAAAAAAGG CGATTTTTCC GCCTAAAACT CTTTAAAACT TATTAAAACT
    11641 CTTAAAACCC GCCTGGCCTG TGCATAACTG TCTGGCCAGC GCACAGCCGA AGAGCTGCAA
    11701 AAAGCGCCTA CCCTTCGGTC GCTGCGCTCC CTACGCCCCG CCGCTTCGCG TCGGCCTATC
    11761 GCGGCCGCTG GCCGCTCAAA AATGGCTGGC CTACGGCCAG GCAATCTACC AGGGCGCGGA
    11821 CAAGCCGCGC CGTCGCCACT CGACCGCCGG CGCCCACATC AAGGCACCCT GCCTCGCGCG
    11881 TTTCGGTGAT GACGGTGAAA ACCTCTGACA CATGCAGCTC CCGGAGACGG TCACAGCTTG
    11941 TCTGTAAGCG GATGCCGGGA GCAGACAAGC CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG
    12001 GTGTCGGGGC GCAGCCATGA CCCAGTCACG TAGCGATAGC GGAGTGTATA CTGGCTTAAC
    12061 TATGCGGCAT CAGAGCAGAT TGTACTGAGA GTGCACCATA TGCGGTGTGA AATACCGCAC
    12121 AGATGCGTAA GGAGAAAATA CCGCATCAGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG
    12181 CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG
    12241 TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG
    12301 GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC
    12361 GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA
    12421 TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT
    12481 ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC
    12541 TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC
    12601 CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA
    12661 AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT
    12721 GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA
    12781 GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT
    12841 TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT
    12901 ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT
    12961 CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGC ATTCTAGGTA CTAAAACAAT
    13021 TCATCCAGTA AAATATAATA TTTTATTTTC TCCCAATCAG GCTTGATCCC CAGTAAGTCA
    13081 AAAAATAGCT CGACATACTG TTCTTCCCCG ATATCCTCCC TGATCGACCG GACGCAGAAG
    13141 GCAATGTCAT ACCACTTGTC CGCCCTGCCG CTTCTCCCAA GATCAATAAA GCCACTTACT
    13201 TTGCCATCTT TCACAAAGAT GTTGCTGTCT CCCAGGTCGC CGTGGGAAAA GACAAGTTCC
    13261 TCTTCGGGCT TTTCCGTCTT TAAAAAATCA TACAGCTCGC GCGGATCTTT AAATGGAGTG
    13321 TCTTCTTCCC AGTTTTCGCA ATCCACATCG GCCAGATCGT TATTCAGTAA GTAATCCAAT
    13381 TCGGCTAAGC GGCTGTCTAA GCTATTCGTA TAGGGACAAT CCGATATGTC GATGGAGTGA
    13441 AAGAGCCTGA TGCACTCCGC ATACAGCTCG ATAATCTTTT CAGGGCTTTG TTCATCTTCA
    13501 TACTCTTCCG AGCAAAGGAC GCCATCGGCC TCACTCATGA GCAGATTGCT CCAGCCATCA
    13561 TGCCGTTCAA AGTGCAGGAC CTTTGGAACA GGCAGCTTTC CTTCCAGCCA TAGCATCATG
    13621 TCCTTTTCCC GTTCCACATC ATAGGTGGTC CCTTTATACC GGCTGTCCGT CATTTTTAAA
    13681 TATAGGTTTT CATTTTCTCC CACCAGCTTA TATACCTTAG CAGGAGACAT TCCTTCCGTA
    13741 TCTTTTACGC AGCGGTATTT TTCGATCAGT TTTTTCAATT CCGGTGATAT TCTCATTTTA
    13801 GCCATTTATT ATTTCCTTCC TCTTTTCTAC AGTATTTAAA GATACCCCAA GAAGCTAATT
    13861 ATAACAAGAC GAACTCCAAT TCACTGTTCC TTGCATTCTA AAACCTTAAA TACCAGAAAA
    13921 CAGCTTTTTC AAAGTTGTTT TCAAAGTTGG CGTATAACAT AGTATCGACG GAGCCGATTT
    13981 TGAAACCGCG GTGATCACAG GCAGCAACGC TCTGTCATCG TTACAATCAA CATGCTACCC
    14041 TCCGCGAGAT CATCCGTGTT TCAAACCCGG CAGCTTAGTT GCCGTTCTTC CGAATAGCAT
    14101 CGGTAACATG AGCAAAGTCT GCCGCCTTAC AACGGCTCTC CCGCTGACGC CGTCCCGGAC
    14161 TGATGGGCTG CCTGTATCGA GTGGTGATTT TGTGCCGAGC TGCCGGTCGG GGAGCTGTTG
    14221 GCTGGCTGG
//
