LOCUS       Exported               11808 bp ds-DNA     circular SYN 07-APR-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 7, 2023 from SnapGene 4.0.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11808
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          5514..5891
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=source:synthetic DNA construct
                     /note="/ApEinfo_fwdcolor=#00DF87
                     /ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0
                     /ApEinfo_hidden=
                     /ApEinfo_label=source:synthetic DNA construct
                     /ApEinfo_revcolor=#00DF87
                     /locus_tag=source:synthetic DNA construct"
     misc_feature    985..1618
                     /locus_tag="LTR"
                     /label=LTR
                     /note="/ApEinfo_fwdcolor=#bf7e69
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_label=LTR
                     /ApEinfo_revcolor=green"
     misc_feature    1670..1807
                     /locus_tag="PSI"
                     /label=PSI
                     /note="/ApEinfo_fwdcolor=cyan
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_label=PSI
                     /ApEinfo_revcolor=green"
     misc_feature    2294..2498
                     /locus_tag="RRE"
                     /label=RRE
                     /note="/ApEinfo_fwdcolor=cyan
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_label=RRE
                     /ApEinfo_revcolor=green"
     misc_feature    2890..2892
                     /locus_tag="3' spice site"
                     /label=3' spice site
                     /note="/ApEinfo_fwdcolor=cyan
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_label=3' spice site
                     /ApEinfo_revcolor=green"
     misc_feature    2971..2993
                     /locus_tag="end of env from NL4-3"
                     /label=end of env from NL4-3
                     /note="/ApEinfo_fwdcolor=#ba25ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_label=end of env from NL4-3
                     /ApEinfo_revcolor=green"
     promoter        3203..3713
                     /locus_tag="hPGK promoter"
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter
                     /ApEinfo_fwdcolor=#346ee0
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_label=hPGK promoter
                     /ApEinfo_revcolor=#346ee0"
     CDS             3732..4754
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /locus_tag="HygR"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin
                     /ApEinfo_fwdcolor=#e9d024
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_label=HygR
                     /ApEinfo_revcolor=#e9d024"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     misc_feature    4761..4826
                     /locus_tag="P2A"
                     /label=P2A
                     /note="/ApEinfo_fwdcolor=#ff89d8
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_label=P2A
                     /ApEinfo_revcolor=#ff1381"
     CDS             4833..5507
                     /codon_start=1
                     /locus_tag="mRFP1"
                     /product="monomeric derivative of DsRed (Campbell et al.,
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized
                     /ApEinfo_fwdcolor=#ff7d78
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_label=mRFP1
                     /ApEinfo_revcolor=#e9d024"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(4963..4981)
                     /locus_tag="mCherry-R"
                     /label=mCherry-R
                     /note="mCherry, reverse primer
                     /ApEinfo_fwdcolor=#14c0bd
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_label=mCherry-R
                     /ApEinfo_revcolor=#4ec02b"
     primer_bind     5231..5250
                     /locus_tag="mCherry-F"
                     /label=mCherry-F
                     /note="mCherry, forward primer
                     /ApEinfo_fwdcolor=#14c0bd
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_label=mCherry-F
                     /ApEinfo_revcolor=#4ec02b"
     primer_bind     5425..5448
                     /locus_tag="DsRed1-C"
                     /label=DsRed1-C
                     /note="DsRed1, forward primer
                     /ApEinfo_fwdcolor=#14c0bd
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_label=DsRed1-C
                     /ApEinfo_revcolor=#4ec02b"
     CDS             5514..5888
                     /codon_start=1
                     /gene="MAP1LC3B"
                     /locus_tag="LC3B"
                     /product="human LC3 protein"
                     /label=LC3B
                     /note="Atg8 family member found on autophagosomes
                     /ApEinfo_fwdcolor=#72fcd5
                     /ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0
                     /ApEinfo_label=LC3B
                     /ApEinfo_revcolor=#e9d024"
                     /translation="MPSEKTFKQRRTFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLP
                     VLDKTKFLVPDHVNMSELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYESEKD
                     EDGFLYMVYASQETFGMKLSV"
     misc_feature    5892..5897
                     /locus_tag="natural XhoI site from NL4-3"
                     /label=natural XhoI site from NL4-3
                     /note="/ApEinfo_fwdcolor=#80ff12
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_label=natural XhoI site from NL4-3
                     /ApEinfo_revcolor=green"
     misc_feature    6079..6340
                     /locus_tag="SIN LTR"
                     /label=SIN LTR
                     /note="/ApEinfo_fwdcolor=#bf7e69
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_label=SIN LTR
                     /ApEinfo_revcolor=green"
ORIGIN
        1 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
       61 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
      121 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
      181 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
      241 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
      301 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
      361 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
      421 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
      481 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
      541 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
      601 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
      661 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
      721 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
      781 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
      841 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
      901 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
      961 aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata
     1021 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
     1081 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     1141 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
     1201 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
     1261 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
     1321 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
     1381 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
     1441 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     1501 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     1561 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     1621 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     1681 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     1741 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     1801 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1861 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1921 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1981 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     2041 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     2101 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     2161 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     2221 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     2281 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     2341 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     2401 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     2461 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     2521 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     2581 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     2641 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     2701 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     2761 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     2821 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2881 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2941 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
     3001 tatcgccgaa ttcacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     3061 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     3121 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     3181 atccactttg gctgatacgc gtggggttgg ggttgcgcct tttccaaggc agccctgggt
     3241 ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa acgcagcggc gccgaccctg
     3301 ggtctcgcac attcttcacg tccgttcgca gcgtcacccg gatcttcgcc gctacccttg
     3361 tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc ttgcggttcg
     3421 cggcgtgccg gacgtgacaa acggaagccg cacgtctcac tagtaccctc gcagacggac
     3481 agcgccaggg agcaatggca gcgcgccgac cgcgatgggc tgtggccaat agcggctgct
     3541 cagcagggcg cgccgagagc agcggccggg aaggggcggt gcgggaggcg gggtgtgggg
     3601 cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag cctccggagc
     3661 gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga cctctctccc cagccgcggg
     3721 ctagcgccac catgaaaaag cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg
     3781 aaaagttcga cagcgtctcc gacctgatgc agctctcgga gggcgaagaa tctcgtgctt
     3841 tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc gccgatggtt
     3901 tctacaaaga tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg attccggaag
     3961 tgcttgacat tggggaattt agcgagagcc tgacctattg catctcccgc cgtgcacagg
     4021 gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg
     4081 aggccatgga tgcgatcgct gcggccgatc ttagccagac gagcgggttc ggcccattcg
     4141 gaccgcaagg aatcggtcaa tacactacat ggcgtgattt catatgcgcg attgctgatc
     4201 cccatgtgta tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg
     4261 ctctcgatga gctgatgctt tgggccgagg actgccccga agtccggcac ctcgtgcacg
     4321 cggatttcgg ctccaacaat gtcctgacgg acaatggccg cataacagcg gtcattgact
     4381 ggagcgaggc gatgttcggg gattcccaat acgaggtcgc caacatcttc ttctggaggc
     4441 cgtggttggc ttgtatggag cagcagacgc gctacttcga gcggaggcat ccggagcttg
     4501 caggatcgcc gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga
     4561 gcttggttga cggcaatttc gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg
     4621 tccgatccgg agccgggact gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct
     4681 ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc agcactcgtc
     4741 cgagggcaaa ggaacgtacg ggaagcggag ctactaactt cagcctgctg aagcaggctg
     4801 gagacgtgga ggagaaccct ggacctggat ccatggcctc ctccgaggac gtcatcaagg
     4861 agttcatgcg cttcaaggtg cgcatggagg gctccgtgaa cggccacgag ttcgagatcg
     4921 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
     4981 agggcggccc cctgcccttc gcctgggaca tcctgtcccc tcagttccag tacggctcca
     5041 aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg
     5101 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg
     5161 actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc
     5221 cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc accgagcgga
     5281 tgtaccccga ggacggcgcc ctgaagggcg agatcaagat gaggctgaag ctgaaggacg
     5341 gcggccacta cgacgccgag gtcaagacca cctacatggc caagaagccc gtgcagctgc
     5401 ccggcgccta caagaccgac atcaagctgg acatcacctc ccacaacgag gactacacca
     5461 tcgtggaaca gtacgagcgc gccgagggcc gccactccac cggcgcctct agaatgccgt
     5521 cggagaagac cttcaagcag cgccgcacct tcgaacaaag agtagaagat gtccgactta
     5581 ttcgagagca gcatccaacc aaaatcccgg tgataataga acgatacaag ggtgagaagc
     5641 agcttcctgt tctggataaa acaaagttcc ttgtacctga ccatgtcaac atgagtgagc
     5701 tcatcaagat aattagaagg cgcttacagc tcaatgctaa tcaggccttc ttcctgttgg
     5761 tgaacggaca cagcatggtc agcgtctcca caccaatctc agaggtgtat gagagtgaga
     5821 aagatgaaga tggattcctg tacatggtct atgcctccca ggagacgttc gggatgaaat
     5881 tgtcagtgta actcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc
     5941 taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt
     6001 cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     6061 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aaaatcgtcg
     6121 agagatgctg catataagca gctgcttttt gcttgtactg ggtctctctg gttagaccag
     6181 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     6241 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     6301 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt
     6361 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gccttgacat
     6421 tataatagat ttagcaggaa ttgaactagg agtggagcac acaggcaaag ctgcagaagt
     6481 acttggaaga agccaccaga gatactcacg attctgcaca tacctggcta atcccagatc
     6541 ctaaggatta cattaagttt actaacattt atataatgat ttatagttta aagtataaac
     6601 ttatctaatt tactattctg acagatatta attaatcctc aaatatcata agagatgatt
     6661 actattatcc ccatttaaca caagaggaaa ctgagaggga aagatgttga agtaattttc
     6721 ccacaattac agcatccgtt agttacgact ctatgatctt ctgacacaaa ttccatttac
     6781 tcctcaccct atgactcagt cgaatatatc aaagttatgg acattatgct aagtaacaaa
     6841 ttaccctttt atatagtaaa tactgagtag attgagagaa gaaattgttt gcaaacctga
     6901 atagcttcaa gaagaagaga agtgaggata agaataacag ttgtcattta acaagtttta
     6961 acaagtaact tggttagaaa gggattcaaa tgcataaagc aagggataaa tttttctggc
     7021 aacaagacta tacaatataa ccttaaatat gacttcaaat aattgttgga acttgataaa
     7081 actaattaaa tattattgaa gattatcaat attataaatg taatttactt ttaaaaaggg
     7141 aacatagaaa tgtgtatcat tagagtagaa aacaatcctt attatcacaa tttgtcaaaa
     7201 caagtttgtt attaacacaa gtagaatact gcattcaatt aagttgactg cagattttgt
     7261 gttttgttaa aattagaaag agataacaac aatttgaatt attgaaagta acatgtaaat
     7321 agttctacat acgttctttt gacatcttgt tcaatcattg atcgaagttc tttatcttgg
     7381 aagaatttgt tccaaagact ctgaaataag gaaaacaatc tattatatag tctcacacct
     7441 ttgttttact tttagtgatt tcaatttaat aatgtaaatg gttaaaattt attcttctct
     7501 gagatcattt cacattgcag atagaaaacc tgagactggg gtaattttta ttaaaatcta
     7561 atttaatctc agaaacacat ctttattcta acatcaattt ttccagtttg atattatcat
     7621 ataaagtcag ccttcctcat ctgcaggttc cacaacaaaa atccaaccaa ctgtggatca
     7681 aaaatattgg gaaaaaatta aaaatagcaa tacaacaata aaaaaataca aatcagaaaa
     7741 acagcacagt ataacaactt tatttagcat ttacaatcta ttaggtatta taagtaatct
     7801 agccagatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt
     7861 gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc
     7921 atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc
     7981 gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta
     8041 ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaatggt gcactctcag
     8101 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     8161 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     8221 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
     8281 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     8341 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     8401 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     8461 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     8521 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     8581 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     8641 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     8701 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     8761 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     8821 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     8881 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     8941 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     9001 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     9061 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     9121 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     9181 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     9241 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     9301 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     9361 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     9421 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     9481 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     9541 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     9601 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     9661 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     9721 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     9781 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     9841 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     9901 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     9961 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
    10021 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
    10081 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
    10141 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
    10201 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
    10261 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
    10321 atgcagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
    10381 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
    10441 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
    10501 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
    10561 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
    10621 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct tggacacaag
    10681 acaggcttgc gagatatgtt tgagaatacc actttatccc gcgtcaggga gaggcagtgc
    10741 gtaaaaagac gcggactcat gtgaaatact ggtttttagt gcgccagatc tctataatct
    10801 cgcgcaacct attttcccct cgaacacttt ttaagccgta gataaacagg ctgggacact
    10861 tcacatgagc gaaaaataca tcgtcacctg ggacatgttg cagatccatg cacgtaaact
    10921 cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt attgccgtaa gccgtggcgg
    10981 tctgtaccgg gtgcgttact ggcgcgtgaa ctgggtattc gtcatgtcga taccgtttgt
    11041 atttccagct acgatcacga caaccagcgc gagcttaaag tgctgaaacg cgcagaaggc
    11101 gatggcgaag gcttcatcgt tattgatgac ctggtggata ccggtggtac tgcggttgcg
    11161 attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc ggctggtcgt
    11221 ccgctggttg atgactatgt tgttgatatc ccgcaagata cctggattga acagccgtgg
    11281 gatatgggcg tcgtattcgt cccgccaatc tccggtcgct aatcttttca acgcctggca
    11341 ctgccgggcg ttgttctttt taacttcagg cgggttacaa tagtttccag taagtattct
    11401 ggaggctgca tccatgacac aggcaaacct gagcgaaacc ctgttcaaac cccgctttaa
    11461 acatcctgaa acctcgacgc tagtccgccg ctttaatcac ggcgcacaac cgcctgtgca
    11521 gtcggccctt gatggtaaaa ccatccctca ctggtatcgc atgattaacc gtctgatgtg
    11581 gatctggcgc ggcattgacc cacgcgaaat cctcgacgtc caggcacgta ttgtgatgag
    11641 cgatgccgaa cgtaccgacg atgatttata cgatacggtg attggctacc gtggcggcaa
    11701 ctggatttat gagtgggccc cggatctttg tgaaggaacc ttacttctgt ggtgtgacat
    11761 aattggacaa actacctaca gagatttaaa gctctaaggt aatataaa
//
